检索结果-南通市图书馆

Rapid development of double-hit mRNA antibody cocktail against orthopoxviruses

Signal Transduction and Targeted Therapy 2024年第4期9卷 1768-1776页

作者： Hang Chi Suo-Qun Zhao Ru-Yi Chen Xing-Xing Suo rong-rong zhang Wen-Hui Yang Dong-Sheng Zhou Min Fang Bo Ying Yong-Qiang Deng Cheng-Feng Qin State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyAMMS100071BeijingChina College of Life Sciences Inner Mongolia Agricultural UniversityHohhot010018Inner MongoliaChina School of Life Sciences Henan UniversityKaifeng475004HenanChina Suzhou Abogen Biosciences Co. LtdSuzhou215123JiangsuChina Research Unit of Discovery and Tracing of Natural Focus Diseases Chinese Academy of Medical Sciences100071BeijingChina

The Orthopoxvirus genus,especially variola virus(VARV),monkeypox virus(MPXV),remains a significant public health threat worldwide.The development of therapeutic antibodies against orthopoxviruses is largely hampered by the high cost of antibody engineering and manufacturing processes.mRNA-encoded antibodies have emerged as a powerful and universal platform for rapid antibody production.Herein,by using the established lipid nanoparticle(LNP)-encapsulated mRNA platform,we constructed four mRNA combinations that encode monoclonal antibodies with broad neutralization activities against orthopoxviruses.In vivo characterization demonstrated that a single intravenous injection of each LNP-encapsulated mRNA antibody in mice resulted in the rapid production of neutralizing antibodies.More importantly,mRNA antibody treatments showed significant protection from weight loss and mortality in the vaccinia virus(VACV)lethal challenge mouse model,and a unique mRNA antibody cocktail,Mix2a,exhibited superior in vivo protection by targeting both intracellular mature virus(IMV)-form and extracellular enveloped virus(EEV)-form viruses.In summary,our results demonstrate the proof-of-concept production of orthopoxvirus antibodies via the LNP-mRNA platform,highlighting the great potential of tailored mRNA antibody combinations as a universal strategy to combat orthopoxvirus as well as otheremerging viruses.

关键词： proof universal neutral

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A new species of Bostrychus (Gobiiformes: Eleotridae) from the East China Sea

引用

Zoological Research(Diversity and Conservation) 2024年第1期1卷 82-85页

作者： rong-rong zhang Kuan Yang Dian Luo Shao-Xiong Ding College of Ocean and Earth Sciences Xiamen UniversityXiamenFujian 361102China

DEAR EDITOR,Bostrychus,a genus in the family Eleotridae of the order Gobiiformes,was first established by Lacepède in 1801(Buffon,1801).Bostrychus currently contains seven recognized species,including two recent additions(B.microphthalmos and B.scalaris)described in 2005(Hoese&Kottelat,2005)and 2008(Larson,2008),respectively.The natural range of Bostrychus species extends from East Asia to Australia,with the exception of B.africanus,Steindachner,1879,which is distributed in West Africa(Herre,1946).Among the recognized species,B.sinensis,B.zonatus,and B.africanus are relatively widespread,inhabiting diverse areas from estuaries to freshwater streams,while B.scalaris is only found at a single mangrove site in the Selangor State of Malaysia(Larson,2008).The remaining three species exhibit a high degree of habitat specialization and are highly localized(Hoese&Kottelat,2005):B.microphthalmos inhabits a cave stream in the Maros karst of southern Sulawesi,B.aruensis is confined to freshwater environments in the Aru Islands of Indonesia,and B.strigogenys is found only in freshwater areas in southern Papua New Guinea and Irian Jaya.

关键词： East karst environments

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A new seamoth species of Pegasus(Syngnathiformes:Pegasidae)from the East China Sea

引用

Zoological Research 2022年第4期43卷 675-678页

作者： Ying-Yi zhang rong-rong zhang Shao-Bo Ma Shuai-Shuai Liu Qiang Lin Xin Wang CAS Key Laboratory of Tropical Marine Bio-resources and Ecology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhouGuangdong 510301China Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) GuangzhouGuangdong 511458China State Key Laboratory of Marine Environmental Science/College of the Environment and Ecology Xiamen UniversityXiamenFujian 361102China University of the Chinese Academy of Sciences Beijing 100049China Laboratory for Marine Fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)QingdaoShandong 266237China

DEAR EDITOR,The Pegasidae family,which includes seven species of seamoths,is widely distributed in the temperate and tropical waters of the Indo-Pacific,ranging from South Africa to Hawaii.In this study,we describe a new species of seamoth,Pegasus sinensis sp.nov.,based on morphological and molecular characterization of 14 specimens collected from the surrounding waters of Xiamen City,Fujian Province,China.The new species differs from closely related P.volitans in both the density and color of spots present on the dorsal and lateral body surfaces.

关键词： Xiamen asi temperate

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A highly immunogenic live-attenuated vaccine candidate prevents SARS-CoV-2 infection and transmission in hamsters

引用

The Innovation 2022年第2期3卷 126-135页

作者： Xiao-Feng Li Zhen Cui Hang Fan Qi Chen Lei Cao Hong-Ying Qiu Na-Na zhang Yan-Peng Xu rong-rong zhang Chao Zhou Qing Ye Yong-Qiang Deng Yan Guo Si Qin Kaiyue Fan Lei Wang Zijing Jia Yujun Cui Xiangxi Wang Cheng-Feng Qin Department of Virology State Key Laboratory of Pathogen and BiosecurityBeijing Institute of Microbiology and EpidemiologyAcademy of Military Medical SciencesBeijing 100071China CAS Key Laboratory of Infection and Immunity National Laboratory of MacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China School of Medicine Tsinghua UniversityBeijing 100084China Research Unit of Discovery and Tracing of Natural Focus Diseases Chinese Academy of Medical SciencesBeijing 100071China

The highly pathogenic and readily transmissible SARS-CoV-2 has caused a global coronavirus pandemic,urgently requiring effective countermeasures against its rapid expansion.All available vaccine platforms are being used to generate safe and effective COVID-19 vaccines.Here,we generated a live-attenuated candidate vaccine strain by serial passaging of a SARSCoV-2 clinical isolate in Vero cells.

关键词： vaccine attenuated infection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Generating colorful carrot germplasm through metabolic engineering of betalains pigments

引用

Horticulture Research 2023年第4期10卷 63-70页

作者： Yuan-Jie Deng Ao-Qi Duan Hui Liu Ya-Hui Wang rong-rong zhang Zhi-Sheng Xu Ai-Sheng Xiong National Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East ChinaCollege of HorticultureNanjing Agricultural University1 WeigangNanjing 210095China

Betalains are tyrosine-derived plant pigments exclusively found in the Caryophyllales order and some higher fungi and generally classified into two groups:red-violet betacyanins and yellow-orange betaxanthins.Betalains attract great scientific and economic interest because of their relatively simple biosynthesis pathway,attractive colors and health-promoting properties.Co-expressing two core genes BvCYP76AD1 and BvDODA1 with or without a glycosyltransferase gene MjcDOPA5GT allowed the engineering of carrot(an important taproot vegetable)to produce a palette of unique colors.The highest total betalains content,943.2μg·g^(−1) DW,was obtained in carrot taproot transformed with p35S:RUBY which produces all of the necessary enzymes for betalains synthesis.Root-specific production of betalains slightly relieved tyrosine consumption revealing the possible bottleneck in betalains production.Furthermore,a unique volcano-like phenotype in carrot taproot cross-section was created by vascular cambium-specific production of betalains.The betalains-fortified carrot in this study is thus anticipated to be used as functional vegetable and colorful carrot germplasm in breeding to promote health.

关键词： germplasm breeding orange

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Isolation and chemotaxonomic significance of stenine- and stemoninine-type alkaloids from the roots of Stemona tuberosa

引用

Chinese Chemical Letters 2014年第9期25卷 1252-1255页

作者： rong-rong zhang Hai-Yan Tian Yi Wu Xiao-Hui Sun Jian-Long zhang Zhi-Guo Ma Ren-Wang Jiang Institute of Traditional Chinese Medicine and Natural Products College of PharmacyJinan University Zhongshan Torch Polytechnic Shenzhen Engineering Laboratory of Lingnan Herbal Resource Development and Application Shenzhen Institute for Drug Control

One new stenine-type alkaloid, tuberostemonine D （1）, together with five known stenine-type alkaloids （2-6） and two known stemoninine-type alkaloids （7 and 8）, were isolated from the roots of Stemona tuberosa. Their structures were elucidated by extensive spectroscopic methods （IR, UV, MS, 1D and 2D NMR）, and the structure of I was further confirmed by X-ray diffraction analysis. First simultaneous isolation ofstenine- and stemoninine-type alkaloids not only added a new chemical type and increased the chemical diversity of Stemona tuberosa, but also provided chemotaxonomic clues for the close relationship between genera Stemona and Stichoneuron, and favored retaining them in the same family.

关键词： Sternona tuberosa Alkaloid Tuberostemonine-type

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Transient acquisition of cross-species infectivity during the evolution of SARS-CoV-2

引用

National Science Review 2021年第11期8卷 85-87页

作者： Qi Chen Xing-Yao Huang Meng-Xu Sun Rui-Ting Li Hongjing Gu Ying Tian rong-rong zhang Dan Luo Chao Zhou Yifei zhang Tianshu Cao Na-Na zhang Yong-Qiang Deng Xiao-Feng Li Cheng-Feng Qin State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyAMMS Research Unit of Discovery and Tracing of Natural Focus Diseases Chinese Academy of Medical Sciences

Since the coronavirus disease 2019(COVID-19) pandemic began,its causative agent,severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2),has spread worldwide.During the global transmission of SARS-CoV-2,mutations in the viral genome have gradually accumulated and have led to the emergence of variants.These emerging variants,

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Construction and characterization of UAA-controlled recombinant Zika virus by genetic code expansion

引用

Science China(Life Sciences) 2021年第1期64卷 171-173页

作者： rong-rong zhang Qing Ye Xiao-Feng Li Yong-Qiang Deng Yan-Peng Xu Xing-Yao Huang Qing Xia Cheng-Feng Qin Graduate School Anhui Medical UniversityHefei230032China Department of Virology State Key Laboratory of Pathogen and BiosecurityBeijing Institute of Microbiology and EpidemiologyBeijing100071China Department of Chemical Biology State Key Laboratory of Natural and Biomimetic DrugsSchool of Pharmaceutical SciencesPeking UniversityBeijing100191China

Dear Editor,Genetic code expansion based on orthogonal tRNA-aminoacyl tRNA synthetase is an emerging technology to sitespecifically incorporate unnatural amino acids(UAAs)into viral proteins to produce UAA-controlled ... 详细信息

关键词： expansion characterization incorporate

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Infection and Pathogenicity of SARS-CoV-2 Variant B.1.351 in hACE2 Mice

引用

Virologica Sinica 2021年第5期36卷 1232-1235页

作者： Qi Chen Xing-Yao Huang Ying Tian Changfa Fan Mengxu Sun Chao Zhou Ruiting Li rong-rong zhang Guizhen Wu Cheng-Feng Qin State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyAMMSBeijing 100071China School of Basic Medical Sciences Anhui Medical UniversityHefei 230032China Division of Animal Model Research Institute for Laboratory Animal ResourcesNational Institutes for Food and Drug ControlBeijing 102629China National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China

Dear Editor,Since the outbreak of COVID-19 at the end of 2019,its causative agent SARS-Co V-2 has been spreading around the world for one and half a year.During the long global circulation of SARS-Co V-2,mutations in ... 详细信息

关键词： emerged accumulated SARS

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Telomere-to-telomere carrot (Daucus carota) genome assembly reveals carotenoid characteristics

引用

Horticulture Research 2023年第7期10卷 112-124页

作者： Ya-Hui Wang Pei-Zhuo Liu Hui Liu rong-rong zhang Yi Liang Zhi-Sheng Xu Xiao-Jie Li Qing Luo Guo-Fei Tan Guang-Long Wang Ai-Sheng Xiong State Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East ChinaCollege of HorticultureNanjing Agricultural UniversityNanjingJiangsu 210095China Institute of Horticulture Guizhou Academy of Agricultural SciencesGuiyangGuizhou 550025China School of Life Science and Food Engineering Huaiyin Institute of TechnologyHuaianJiangsu 223003China Beijing Vegetable Research Center Beijing Academy of Agriculture and Forestry SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural Crops in North ChinaBeijing 100097China

Carrot(Daucus carota)is an Apiaceae plant with multi-colored fleshy roots that provides a model system for carotenoid research.In this study,we assembled a 430.40 Mb high-quality gapless genome to the telomere-to-telomere(T2T)level of“Kurodagosun”carrot.In total,36268 genes were identified and 34961 of them were functionally annotated.The proportion of repeat sequences in the genome was 55.3%,mainly long terminal repeats.Depending on the coverage of the repeats,14 telomeres and 9 centromeric regions on the chromosomes were predicted.A phylogenetic analysis showed that carrots evolved early in the family Apiaceae.Based on the T2T genome,we reconstructed the carotenoid metabolic pathway and identified the structural genes that regulate carotenoid biosynthesis.Among the 65 genes that were screened,9 were newly identified.Additionally,some gene sequences overlapped with transposons,suggesting replication and functional differentiation of carotenoid-related genes during carrot evolution.Given that some gene copies were barely expressed during development,they might be functionally redundant.Comparison of 24 cytochrome P450 genes associated with carotenoid biosynthesis revealed the tandem or proximal duplication resulting in expansion of CYP gene family.These results provided molecular information for carrot carotenoid accumulation and contributed to a new genetic resource.

关键词： carrot sequences characteristics

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：