Generating colorful carrot germplasm through metabolic engineering of betalains pigments
作者机构：National Key Laboratory of Crop Genetics and Germplasm Enhancement and UtilizationMinistry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East ChinaCollege of HorticultureNanjing Agricultural University1 WeigangNanjing 210095China
出 版 物：《Horticulture Research》 (园艺研究（英文）)
年 卷 期：2023年第10卷第4期
页 面：63-70页
核心收录：
学科分类：09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学]
基 金：supported by the National Natural Science Foundation of China(32072563) Key Research and Development Projects of Ningxia Hui Autonomous Region(No.2022BBF02008) Priority Academic Program Development of Jiangsu Higher Education Institutions(Grant No.PAPD)
摘 要：Betalains are tyrosine-derived plant pigments exclusively found in the Caryophyllales order and some higher fungi and generally classified into two groups:red-violet betacyanins and yellow-orange *** attract great scientific and economic interest because of their relatively simple biosynthesis pathway,attractive colors and health-promoting ***-expressing two core genes BvCYP76AD1 and BvDODA1 with or without a glycosyltransferase gene MjcDOPA5GT allowed the engineering of carrot(an important taproot vegetable)to produce a palette of unique *** highest total betalains content,943.2μg·g^(−1) DW,was obtained in carrot taproot transformed with p35S:RUBY which produces all of the necessary enzymes for betalains ***-specific production of betalains slightly relieved tyrosine consumption revealing the possible bottleneck in betalains ***,a unique volcano-like phenotype in carrot taproot cross-section was created by vascular cambium-specific production of *** betalains-fortified carrot in this study is thus anticipated to be used as functional vegetable and colorful carrot germplasm in breeding to promote health.
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