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Inhibiting ceramide synthase 5 expression in microglia decreases neuroinflammation after spinal cord injury

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Neural Regeneration Research 2025年第10期20卷 2955-2968页

作者： wei Zhang Yubao Lu Ruoqi Shen yingjie Wu chenrui Liu Xingxing Fang Liangming Zhang Bin Liu Limin rong Department of Spine Surgery The Third Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina National Medical Products Administration(NMPA)Key Laboratory for Quality Research and Evaluation of Cell Products GuangzhouGuangdong ProvinceChina Guangdong Provincial Center for Quality Control of Minimally Invasive Spine Surgery GuangzhouGuangdong ProvinceChina Guangdong Provincial Center for Engineering and Technology Research of Minimally Invasive Spine Surgery GuangzhouGuangdong ProvinceChina

Microglia,the resident monocyte of the central nervous system,play a crucial role in the response to spinal cord ***,the precise mechanism remains *** investigate the molecular mechanisms by which microglia regulate the neuroinflammatory response to spinal cord injury,we performed single-cell RNA sequencing dataset analysis,focusing on changes in microglial *** found that the MG1 subpopulation emerged in the acute/subacute phase of spinal cord injury and expressed genes related to cell pyroptosis,sphingomyelin metabolism,and neuroinflammation at high ***,we established a mouse model of contusive injury and performed intrathecal injection of siRNA and molecular inhibitors to validate the role of ceramide synthase 5 in the neuroinflammatory responses and pyroptosis after spinal cord ***,we established a PC12-BV2 cell co-culture system and found that ceramide synthase 5 and pyroptosis-associated proteins were highly expressed to induce the apoptosis of neuron *** ceramide synthase 5 expression in a mouse model of spinal cord injury effectively reduced ***,ceramide synthase 5-induced pyroptosis was dependent on activation of the NLRP3 signaling *** ceramide synthase 5 expression in microglia in vivo reduced neuronal apoptosis and promoted recovery of neurological ***2g7 formed a“bridge”between sphingolipid metabolism and ceramide synthase 5-mediated cell death by inhibiting the NLRP3 signaling ***,these findings suggest that inhibiting ceramide synthase 5 expression in microglia after spinal cord injury effectively suppressed microglial pyroptosis mediated by NLRP3,thereby exerting neuroprotective effects.

关键词： ceramide synthase 5 gasdermin D microglia neuroinflammation NLRP3 nuclear factor kappa B Pla2g7 pyroptosis sphingomyelin metabolism spinal cord injury

Preparation and Enantioseparation Property of Chiral Stationary Phases Based on Cellulose Tris(3,5-dimethylbenzoate)——A New Way to Prepare Polysaccharide-coating Type Chiral Stationary Phases

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Chinese Journal of Polymer Science 2014年第4期32卷 458-466页

作者： wei chen rong Duan Qing-chun Fan 柏正武黄少华 Key Laboratory of Green Chemical Process of Ministry of Education Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical TechnologyWuhan Institute of Technology Key Laboratory of Biofuels of the Chinese Academy of Sciences Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of Sciences

A new method to prepare polysaccharide-coating type chiral stationary phases （CSPs） was developed in this work. As a typical example, naked silica gel was coated by cellulose, which was then derivatized with 3,5-dimethylbenzoyl chloride to afford cellulose tris（3,5-dimethylbenzoate）-silica gel （CTDBS） complex. The silanols on CTDBS were end-capped with 3- aminopropyltriethoxysilane to obtain CSP 1. The amino groups on CSP 1 were further end-capped with 3,5-dimethylbenzoyl chloride to give CSP 2. The silanols on CTDBS were end-capped with methyltrimethoxysilane to yield CSP 3. CSPs 1-3 were characterized by FTIR, solid-state 13C-NMR and elemental analysis. The enantioseparation abilities of CSPs 1-3 were evaluated with structurally various chiral analytes. The enantioseparation results demonstrated that the end-capping moieties on CSPs 1 and 2 significantly affected enantioseparation. In addition, the effect of the structures of chiral analytes and end- capping moieties on the retention factors and the resolutions was discussed.

关键词： Cellulose tris(3,5-dimethylbenzoate) Chiral recognition Chiral stationary phase High-performance liquidchromatography.

Microstructure and properties of AgCu/2 wt%Ag-added Sn-Pb solder/CuBe joints fabricated by vapor phase soldering

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Rare Metals 2022年第6期41卷 1983-1988页

作者： wei Liu rong An ying Ding chun-Qing Wang Yan-Hong Tian Kun Shen State Key Laboratory of Welding and Joining Harbin Institute of TechnologyHarbin 150001China School of Engineering and Physical Sciences Heriot-Watt UniversityEdinburgh EH144ASUK Key Laboratory of Miero-systems and Micro-structures Manufacturing Ministry of Education.Harbin Institute of TechnologyHarbin 150001China Beijing Institute of Control Engineering Beijing 100190China

The purpose of this paper is to investigate the effect of 2 wt%Ag addition in Sn-Pb eutectic solder on microstructure and mechanical properties of AgCu/solder/CuBe joint fabricated by vapor phase soldering.63Sn37Pb and 62Sn36Pb2Ag solder pastes were used to join Cu(1.7 wt%-2.5 wt%)Be and Ag(2 wt%-5 wt%)Cu *** fracture modes are observed in 62Sn36Pb2Ag and 63Sn37Pb joints after lateral shear tests at room temperature or 120℃,and shear forces of 62Sn36Pb2Ag joints are far higher than those of 63Sn37Pb *** obvious difference is observed in morphology and thickness of intermetallic compounds(IMCs)at interfaces of the 63Sn37Pb and 62Sn36Pb2Ag *** the two kinds of joints,for-mation of big blocky or plate-like Ag_(3)Sn is ***,many Ag_(3)Sn IMCs particles(1-3 μm in width)in isolated and dispersed distribution are observed within 62Sn36Pb2Ag *** Ag3Sn particles are responsible for the better mechanical properties of 62Sn36Pb2 Ag joints than those of 63Sn37Pb *** results present in this paper may provide a guide for restraining formation of big blocky or plate-like Ag_(3) Sn in joints with Ag pads or thick Ag surface finish on pads by utilizing vapor phase soldering process,and improving mechanical properties of Cu/SnPb/Ag joints by adding Ag in SnPb eutectic solder.

关键词： Microstructure Mechanical property 62Sn36Pb2Ag solder Ag,Sn Vapor phase soldering

π-conjugated chromophore functionalized high-nuclearity titanium-oxo clusters containing structural unit of anatase for photocatalytic selective oxidation of sulfides

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Rare Metals 2024年第4期43卷 1736-1746页

作者： Ling-rong Liao Dong-chun Zheng pin-Xi Ou Qi-Xin Zhao wei-Min Xuan Qi Zheng State Key Laboratory for Modification of Chemical Fibers and Polymer Materials&College of Materials Science and Engineering Donghua UniversityShanghai201620China State Key Laboratory for Modification of Chemical Fibers and Polymer Materials and College of Chemistry and Chemical Engineering Donghua UniversityShanghai 201620China

N-doping has significant influence in manipulating the properties of TiO_(2),and this has stimulated the development of N-donor-functionalized titanium-oxo clusters(TOCs)as molecular models to study the structure-property ***,the structural type and photoresponsive application are still limited for such TOCs,especially regarding the high-nuclearity TOCs that contain structure unit of TiO_(2)for ***,we showed the synthesis of a series of high-nuclearity TOCs 1-3 compounds usingπ-conjugated 1,10-phenanthroline(phen)as chromophore and N-donor functional *** 1 features cocrystal structure composed of one[Ti_(26)]~(2+)and half[Ti_(22)]~(2+),which renders it as the first cocrystallized TOC containing two positively charged species and phen-functionalized TOC showing the highest nuclearity up to 37 Ti *** adjusting the synthetic conditions,the individual{Ti_(22)}and{Ti_(26)}clusters can also be isolated as Compounds 2 and 3,*** core structure of{Ti_(22)}is mainly constructed from four lacunary{Ti_(4)}derived from pentagonal{Ti(Ti)_5}unit,while{Ti_(26)}is built from four complete{Ti(Ti)_5}***,a{Ti_8O_(14)}structure unit of anatase TiO_(2)can be identified in{Ti_(26)}.Based on the unique structural features and proper photophysical and photochemical properties of Compounds 1-3,they are applied for photocatalytic *** to the presence of anatase structure unit in{Ti_(26)}and the synergistic effect from{Ti_(22)}and{Ti_(26)},the catalytic performance presents in the order of Compound 1>Compound 3>Compound *** work provides excellent models to understand the structureproperty relationship from the perspective of cocrystallization and Ti-O binding model and will further promote the application of TOCs as functional catalysts for organic transformation.

关键词： Titanium-oxo cluster Molecular model of anatase TiO_(2) High nuclearity π-conjugated chromophore Photocatalytic sulfoxidation

The protoplast two-way fusions and fusant characteristics of Antrodia cinnamomea and Cordyceps militaris

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Food Science and Human Wellness 2022年第5期11卷 1240-1251页

作者： wei-Kuang Lai yang-Chang Wu Tai-Sheng Yeh chun-Ren Hsieh Yi-Hong Tsai Chien-Kei wei Chi-ying Li ying-chen Lu Fang-rong Chang Graduate Institute of Natural Products Kaohsiung Medical UniversityKaohsiung 807TaiwanChina Graduate Institute of Integrated Medicine China Medical UniversityTaichung 404TaiwanChina Chinese Medicine Research and Development Center China Medical University HospitalTaichung 404TaiwanChina Department of Food Science and Nutrition Meiho UniversityPingtung 912TaiwanChina Department of Food Science National Chiayi UniversityChiayi City 600TaiwanChina Drug Development and Value Creation Research Center Kaohsiung Medical UniversityKaohsiung 807TaiwanChina Department of Medical Research Kaohsiung Medical University HospitalKaohsiung Medical UniversityKaohsiung 807TaiwanChina Department of Marine Biotechnology and Resources National Sun Yat-sen UniversityKaohsiung 804TaiwanChina

This study generated two fused protoplasts of Antrodia cinnamomea and Cordyceps militaris in two *** protoplasts of *** were inactivated by heat to inactivate biochemical processes and enzymatic activities in the cytoplasm,and the protoplasts of *** were inactivated by UV radiation to invalidate their genome function,then they were fused under optimal conditions to get a fusion rate as(7.42±0.8)×10^(-6) fusants/mL;the new fusants were abbreviated as *** the other hand,when *** and *** were treated with heat and UV oppositely using similar experiments,the fusion rate was(9.70±0.68)×10^(-5) fusants/mL,and the new fusants were abbreviated as *** selected each of two best-growing fused colonies Ac-Cm-1,Ac-Cm-2,Cm-Ac-1,and Cm-Ac-2,together with parental *** and ***,and studied their morphology,growth antagonism tests,and genetic relationships by 18 S rRNA *** comparison with the initial cultures of 4 fusants,the yields of adenosine,biomass,cordycepic acid,cordycepin,total polysaccharide,and total triterpenoids were increased up 1.305-50.1563 times in the optimal medium *** gene stability tests,those of the four fusants and their outputs were stabilized within 10 generations.

关键词： Medicinal fungi Protoplast fusion Antrodia cinnamomea Cordyceps militaris Secondary metabolite

Anisotropic Force Ellipsoid Based Multi-axis Motion Optimization of Machine Tools

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Chinese Journal of Mechanical Engineering 2012年第5期25卷 960-967页

作者： PENG Fangyu YAN rong chen wei yang Jianzhong LI Bin State Key Laboratory of Digital Manufacturing Equipment and Technology Huazhong University of Science and Technology Wuhan 430074 China National NC System Engineering Research Center Huazhong University of Science and Technology Wuhan 430074 China

The existing research of the motion optimization of multi-axis machine tools is mainly based on geometric and kinematic constraints, which aim at obtaining minimum-time trajectories and finding obstacle-free paths. In motion optimization, the stiffness characteristics of the whole machining system, including machine tool and cutter, are not considered. The paper presents a new method to establish a general stiffness model of multi-axis machining system. An analytical stiffness model is established by Jacobi and point transformation matrix method. Based on the stiffness model, feed-direction stiffness index is calculated by the intersection of force ellipsoid and the cutting feed direction at the cutter tip. The stiffness index can help analyze the stiffness performance of the whole machining system in the available workspace. Based on the analysis of the stiffness performance, multi-axis motion optimization along tool paths is accomplished by mixed programming using Matlab and Visual C＋＋. The effectiveness of the motion optimization method is verified by the experimental research about the machining performance of a 7-axis 5-linkage machine tool. The proposed research showed that machining stability and production efficiency can be improved by multi-axis motion optimization based on the anisotropic force ellipsoid of the whole machining system.

关键词： stiffness force ellipsoid multi-axis motion optimization

CO_(2) conversion and decarburization kinetics of CO_(2) gas and liquid Fe-C alloy at 1873 K

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Journal of Iron and Steel Research International 2022年第3期29卷 425-433页

作者： Wen-he Wu rong Zhu Zhi-zheng Li chun-yang Wang Guang-sheng wei School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing100083China Research Center of Carbon Dioxide Science University of Science and Technology BeijingBeijing100083China

The reactions between CO_(2) gas and liquid Fe-C alloy with different initial carbon concentrations at 1873 K were investigated using experimental results,thermodynamic equilibrium,and kinetic *** average CO_(2) conversion is greater than 80%when the carbon content ranges from 4.0 to 1.0 wt.%.When the carbon content decreases from 0.5 to 0.1 wt.%,the average CO_(2) conversion diminishes from 83.50%to 40.84%.This proves that CO_(2) gas and liquid Fe-C alloy reaction does not reach equilibrium under experimental conditions compared with the calculated thermodynamic *** the kinetic analysis,it is shown that in the medium-to high-carbon liquid Fe-C alloys,the rate-controlling step involves CO_(2) gas mass transfer or mixed rate-controlling of CO_(2) gas mass transfer with adsorption and dissociation of CO_(2) *** contrast,in the low-carbon liquid Fe-C alloy,carbon mass transfer occurs in the molten *** critical carbon content of the rate-controlling step transformation is 0.7937 wt.%.

关键词： CO_(2)utilization CO_(2)conversion Decarburization kinetics Fe-C alloy Rate-controlling step

Optimality conditions and duality for a class of nondifferentiable multiobjective generalized fractional programming problems

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Applied Mathematics(A Journal of Chinese Universities) 2008年第3期23卷 331-344页

作者： GAO ying rong wei-dong School of Mathematical Sciences Inner Mongolia University Hohhot 010021 China

This paper studies a class of multiobjective generalized fractional programming problems, where the numerators of objective functions are the sum of differentiable function and convex function, while the denominators are the difference of differentiable function and convex function. Under the assumption of Calmness Constraint Qualification the Kuhn-Tucker type necessary conditions for efficient solution are given, and the Kuhn-Tucker type sufficient conditions for efficient solution are presented under the assumptions of （F, α, ρ, d）-V-convexity. Subsequently, the optimality conditions for two kinds of duality models are formulated and duality theorems are proved.

关键词： operations research multiobjective generalized fractional programming optimality condition duality theorem generalized convexity

Cathepsin L plays a key role in SARS-CoV-2 infection in humans and humanized mice and is a promising target for new drug development

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Signal Transduction and Targeted Therapy 2021年第4期6卷 1357-1368页

作者： Miao-Miao Zhao wei-Li yang Fang-Yuan yang Li Zhang wei-Jin Huang wei Hou Chang-Fa Fan rong-Hua Jin ying-Mei Feng You-chun Wang Jin-Kui yang Department of Endocrinology Beijing Tongren HospitalCapital Medical UniversityBeijingChina Division of HIV/AIDS and Sex-Transmitted Virus Vaccines Institute for Biological Product ControlNational Institutes for Food and Drug Control(NIFDC)BeijingChina Department of Science and Technology Beijing Youan HospitalCapital Medical UniversityBeijingChina Division of Animal Model Research Institute for Laboratory Animal ResourcesNational Institutes for Food and Drug ControlBeijingChina

To discover new drugs to combat COVID-19,an understanding of the molecular basis of SARS-CoV-2 infection is urgently ***,for the first time,we report the crucial role of cathepsin L(CTSL)in patients with *** circulating level of CTSL was elevated after SARS-CoV-2 infection and was positively correlated with disease course and ***,SARS-CoV-2 pseudovirus infection increased CTSL expression in human cells in vitro and human ACE2 transgenic mice in vivo,while CTSL overexpression,in turn,enhanced pseudovirus infection in human *** functionally cleaved the SARS-CoV-2 spike protein and enhanced virus entry,as evidenced by CTSL overexpression and knockdown in vitro and application of CTSL inhibitor drugs in ***,amantadine,a licensed anti-influenza drug,significantly inhibited CTSL activity after SARS-CoV-2 pseudovirus infection and prevented infection both in vitro and in ***,CTSL is a promising target for new anti-COVID-19 drug development.

关键词： infection drugs ACE2