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Rice Protein Tagging Project:A Call for International Collaborations on Genome-wide In-Locus Tagging of Rice Proteins

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Molecular Plant 2020年第12期13卷 1663-1665页

作者： Yuming Lu Pamela C.ronald Bin Han jiayang Li jian-Kang Zhu Shanghai Center for Plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghaiChina Department of Plant Pathology and the Genome Center University of CaliforniaDavisCAUSA National Center for Gene Research CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghaiChina State Key Laboratory of Plant Genomics and National Center for Plant Gene Research(Beijing)Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijingChina Department of Horticulture and Landscape Architecture Purdue UniversityWest LafayetteINUSA

In September 1997,the International Rice Genome Sequencing Project was *** initiative pooled the resources of ten nations to obtain the first complete rice genome sequence,and promoted rice research and breeding into the post-genomics era(Li et al.,2018).In 2008,an internationally coordinated project named "RICE2020" was proposed to systematically and fully characterize all rice genes,transcripts,and proteins(Zhang et al.,2008).While genes and their transcripts can be readily characterized by sequencing-and PCR-based methods,the characterization of protein dynamics including protein levels,subcellular localizations,post-transla-tional modifications,and interactions with macromolecules(e.g.,proteins,DNA,RNA,carbohydrates,and lipids)and small molecules(e.g.,metabolites and ligands)is much more challenging and usually requires antibodies that specifically recognize the protein of *** it is very difficult to systematically produce reliable antibodies for the specific recognition of individual plant proteins,a common practice is to transform a tag-fused open reading frame(ORF)of a gene to the corresponding loss-of-function mutant ***,such an ectopically expressed tagged protein may not fully reca-pitulate the properties of the endogenous protein due to the random insertion of the transgene,even when the transgene is expressed under the endogenous gene promoter.A preferred so-lution is to genetically label the coding sequence of the gene of interest,at its in vivo locus,with a sequence encoding a fluores-cent protein tag or an affinity tag such as FLAG or *** "in-locus" protein tagging,as we are naming it here,has been carried out genome-wide in yeast,Caenorhabditis elegans,fly,and mammalian cultured cells,greatly facilitating the characterization of proteins in these organisms(jiang et al.,2018).In 2017,a Genome Tagging Project was launched in mice,aiming to label every protein using a CRISPR/Cas9-based "artificial spermatids"method(

关键词： breeding Genome endogenous

Digital systems : 9th ed.

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Abnormal psychology / 3rd ed.

2004年

作者： Tocci ronald j.

Includes index.

ISBN: (纸本)0131111205

Includes index.

关键词： Digital electronics

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1998年

作者： Comer ronald j.

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热带气旋的自动预报系统(ATCF)

气象科技 1992年第1期20卷 48-54页

作者： ronald j.Miller 赵秀英

美国海军的热带气旋自动预报系统(ATCF)是为关岛联合台风警报中心(jTWC)研制出的一种IBM-AT兼容软件包,它是为协助预报员做出热带气旋预报而设计的.该系统可用图形方式显示出热带气旋的路径、位置坐标和预报资料以及天气场和无线电探空... 详细信息

美国海军的热带气旋自动预报系统(ATCF)是为关岛联合台风警报中心(jTWC)研制出的一种IBM-AT兼容软件包,它是为协助预报员做出热带气旋预报而设计的.该系统可用图形方式显示出热带气旋的路径、位置坐标和预报资料以及天气场和无线电探空仪的观测结果.利用这些基本资料,ATCF还可做出如热带气旋警报这类的报文.个人计算机的计算能力使ATCF得出某些产品,如北太平洋西部的气候平均情况和持续性资料及最佳客观路径指导预报.ATCF可以自动存储所有的热带气旋资料,计算出实时的和过去的风暴预报误差的统计特征,还可使预报人员存取1945年以来的任意一次路径资料.ATCF使热带气旋的预报方法标准化,从而确保预报人员不会忽略掉所应考虑的重要的决定性步骤,ATCF使预报员的人工绘图和在记录板上记录资料这类耗时性工作自动化.该系统虽然是专为jTWC设计的,但其适应性很广,已为美国海军之外的部门选用.

关键词：热带气旋自动预报系统

An implementation strategy to quantify the marine microbial carbon pump and its sensitivity to global change

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National Science Review 2018年第4期5卷 474-480页

作者： Carol Robinson Douglas Wallace jung-Ho Hyun Luca Polimene ronald Benner Yao Zhang Ruanhong Cai Rui Zhang Nianzhi jiao University of East Anglia School of Environmental Sciences Oceanography Department Dalhousie University Hanyang University Department of Marine Science and Convergence Engineering Plymouth Marine Laboratory University of South Carolina Department of Biological Sciences and School of the EarthOcean and Environment State Key Laboratory of Marine Environmental Science and Institute of Marine Microbes and Ecospheres

INTRODUCTION The persistence of the vast pool of recalcitrant dissolved organic carbon(RDOC)in the deep ocean is fundamental to both global carbon cycling and global ***,quantitative understanding of the mechanisms that produce and utilize RDOC is still in its *** from a conceptual framework to quantitative understanding in marine carbon cycling can take the international research community several decades—precious time

关键词： MCP An implementation strategy to quantify the marine microbial carbon pump and its sensitivity to global change

美国黑石河有机污染物输送入海的一维水质模式研究

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海洋与湖沼 1991年第1期22卷 44-49页

作者：郑金树 j.G.quinn C.G.Carey j.T.E1lis 国家海洋局第三海洋研究所厦门361005 美国罗得岛大学海洋研究生院

沿着黑石河沿岸城镇和废物处理场设九个取样站,于1985年夏秋取样三次。在分析和统计的基础上提出黑石河有机污染物一维水质模式,指出黑石河有机污染物的来源、输送和归宿。

关键词：墨石河有机污染物入海水质模式

Reducing Hospital Lengths of Stay: A Five-Year Study

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Case Reports in Clinical Medicine 2021年第6期10卷 160-167页

作者： ronald j. Lagoe james H. Abbott Shelly A. Littau Hospital Executive Council Syracuse NY USA St. joseph’s Hospital Health Center Syracuse NY USA

Hospital length of stay reduction is an important mechanism for improving the outcomes and efficiency of care. This study evaluated the impact of length of stay reduction in the hospitals of Syracuse, New York. Between 2015 and 2020, length of stay reduction saved 7106 inpatient days for adult medicine, and 10,605 patient days for adult surgery in the hospitals. At a conservative late stay rate of $600 per day, $4,263,600 was eliminated for adult medicine and $6,363,000 was eliminated for adult surgery between 2015 and 2020. The study data suggested that the numbers of days saved were the greatest for patients with Major and Extreme severity of illness. At the community level, length of stay reduction can support the efforts of health care providers to free inpatient capacity for patients with Coronavirus and other conditions. It can also generate the efficiency needed to reimburse the costs of care.

关键词： Hospitals Hospital Lengths of Stay Health Care Costs

Dangerous dietary supplements: Garcinia cambogia-associated hepatic failure requiring transplantation

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World journal of Gastroenterology 2016年第45期22卷 10071-10076页

作者： Keri E Lunsford Adam S Bodzin Diego C Reino Hanlin L Wang ronald W Busuttil The Pfleger Liver Institute The Dumont-UCLA Liver Transplant Center Division of Liver and Pancreas Transplantation Department of Surgery David Geffen School of Medicine at UCLA University of California Los Angeles j.C Walter jr. Center for Transplantation Sherrie and Alan Conover Center for Liver Disease and Transplantation Center for Transplantation Immunobiology Houston Methodist Hospital and Research Institute Department of Pathology and Laboratory Medicine David Geffen School of Medicine at UCLA University of California Los Angeles

Commercial dietary supplements are marketed as a panacea for the morbidly obese seeking sustainable weight-loss. Unfortunately, many claims cited by supplements are unsupported and inadequately regulated. Most concerning, how ever, are the associated harmful side effects, often unrecognized by consumers. Garcinia cambogia extract and Garcinia cambogia containing products are some of the most popular dietary supplements currently marketed for weight loss. Here, we report the first known case of fulminant hepatic failure associated with this dietary supplement. One active ingredient in this supplement is hydroxycitric acid, an active ingredient also found in weight-loss supplements banned by the Food and Drug Administration in 2009 for hepatotoxicity. Heightened awareness of the dangers of dietary supplements such as Garcinia cambogia is imperative to prevent hepatoxicity and potential fulminant hepatic failure in additional patients.

关键词： Dietary supplements Fulminant hepatic failure Drug-induced liver injury Liver transplantation Hyroxycitric acid Weight-loss supplements

An XA21-Associated Kinase （OsSERK2） Regulates Immunity Mediated by the XA21 and XA3 Immune Receptors

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Molecular Plant 2014年第5期7卷 874-892页

作者： Xuewei Chena Shimin Zuo Benjamin Schwessinger Mawsheng Chern Patrick E. Canlas Deling Ruan Xiaogang Zhou jing Wang Arsalan Daudi Christopher j. Petzold joshua L. Heazlewood Pamela C. ronald Department of Plant Pathology and the Genome Center University of California Davis CA 95616 USA Rice Research Institute Sichuan Agricultural University Chengdu Sichuan 611130 China joint Bioenergy Institute Emeryville CA 94710 USA Key Laboratory of Crop Genetics and Physiology of jiangsu Province Key Laboratory of Plant Functional Genomics of the Ministry of Education College of Agriculture Yangzhou University Yangzhou 225009 China

The rice XA21 immune receptor kinase and the structurally related XA3 receptor confer immunity to Xanthomonas oryzae pv. oryzae （Xoo）, the causal agent of bacterial leaf blight. Here we report the isolation of OsSERK2 （rice somatic embryogenesis receptor kinase 2） and demonstrate that OsSERK2 positively regulates immunity mediated by XA21 and XA3 as well as the rice immune receptor FLS2 （OsFLS2）. Rice plants silenced for OsSerk2 display altered morphology and reduced sensitivity to the hormone brassinolide. OsSERK2 interacts with the intracellular domains of each immune receptor in the yeast two-hybrid system in a kinase activity-dependent manner. OsSERK2 undergoes bidi- rectional transphosphorylation with XA21 in vitro and forms a constitutive complex with XA21 in vivo. These results demonstrate an essential role for OsSERK2 in the function of three rice immune receptors and suggest that direct interaction with the rice immune receptors is critical for their function. Taken together, our findings suggest that the mechanism of OsSERK2-meditated regulation of rice XA21, XA3, and FLS2 differs from that of AtSERK3/BAK1-mediated regulation of Arabidopsis FLS2 and EFR.

关键词： immune receptor kinases somatic embryogenesis receptor kinase (SERK) immunity Xanthomonas oryzae pv. oryzae rice.