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Portable Diagnostic Platform for Detection of microorganisms Coliforms and ***

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Advances in microbiology 2020年第5期10卷 224-237页

作者： Natália Faria romao Nathalia Aparecida Costa Pereira maribel Elizabeth Funes-Huacca Luciana Gatto Brito Rede de Biodiversidade e Biotecnologia da Amazonia Legal(BIONORTE) Fundacao Universidade Federal de Rondonia(UNIR)Porto VelhoBrazil Department of Chemistry Fundacao Universidade Federal de Rondônia(UNIR)Porto VelhoBrazil Empresa Brasileira de Pesquisa Agropecuária(EmBRAPA) BelémBrazil

Portable diagnostic devices are a viable and low-cost alternative for the detection of pathogens, since they reduce the time of analysis of results availability. Ease of sample collection and quick diagnosis allow this new input to be applied in the diagnosis of the main contaminating microorganisms present in the water. Laboratory tests evaluated the technical viability of the diagnostic device, using commercial strains which were inoculated and optimized in the devices and their growth compared to the conventional method in Petri dishes. Samples of 100 μL bacterial suspension were tested and compared with the traditional sample inoculation method. The device viability was determined by detecting characteristic bacterial colonies in a specific culture medium through the colorimetric development of the obtained colonies. The feasibility assessments allow us to affirm that the device enables both qualitative and quantitative detection of the target bacteria present in liquid samples, and is promising to be applied to assess the quality of water, food and environmental surfaces.

关键词： Portable Device Bacterial Contamination Environment

Towards a Comprehensive Search of Putative Chitinases Sequences in Environmental metagenomic Databases

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Natural Science 2014年第5期6卷 323-337页

作者： Aline S.romao-Dumaresq Adriana m.Fróes Rafael R.C.Cuadrat Floriano P.Silva Alberto m.R.Dávila Laboratório de Biologia Computacional e Sistemas Instituto Oswaldo Cruz(IOC)FIOCRUZRio de JaneiroBrazil Laboratório de Bioquímica de Proteínas e Peptídeos Instituto Oswaldo Cruz(IOC)FIOCRUZRio de JaneiroBrazil Pólo de Biologia Computacional e Sistemas Instituto Oswaldo Cruz(IOC)FIOCRUZRio de JaneiroBrazil

Chitinases catalyze the hydrolysis of chitin, a linear homopolymer of β-(1,4)-linked N-acetylglucosamine. The broad range of applications of chitinolytic enzymes makes their identification and study very promising. metagenomic approaches offer access to functional genes in uncultured representatives of the microbiota and hold great potential in the discovery of novel enzymes, but tools to extensively explore these data are still scarce. In this study, we develop a chitinase mining pipeline to facilitate the comprehensive search of these enzymes in environmental metagenomic databases and also to explore phylogenetic relationships among the retrieved sequences. In order to perform the analyses, UniprotKB fungal and bacterial chitinases sequences belonging to the glycoside hydrolases (GH) family-18, 19 and 20 were used to generate 15 reference datasets, which were then used to generate high quality seed alignments with the mAFFT program. Profile Hidden markov models (pHmms) were built from each seed alignment using the hmmbuild program of HmmER v3.0 package. The best-hit sequences returned by hmmsearch against two environmental metagenomic databases (Community Cyberinfrastructure for Advanced microbial Ecology Research and Analysis—CAmERA and Integrated microbial Genomes—ImG/m) were retrieved and further analyzed. The NJ trees generated for each chitinase dataset showed some variability in the catalytic domain region of the metagenomic sequences and revealed common sequence patterns among all the trees. The scanning of the retrieved metagenomic sequences for chitinase conserved domains/signatures using both the InterPro and the RPS-BLAST tools confirmed the efficacy and sensitivity of our pHmm-based approach in detecting putative chitinases sequences. These analyses provide insight into the potential reservoir of novel molecules in metagenomic databases while supporting the chitinase mining pipeline developed in this work. By using our chitinase mining pipeline, a larg

关键词： Chitinase metagenome pHmm Sequence Search

Analysis of Cocaine and Crack Cocaine via Thin Layer Chromatography Coupled to Easy Ambient Sonic-Spray Ionization mass Spectrometry

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American Journal of Analytical Chemistry 2011年第6期2卷 658-664页

作者： Bruno D.Sabino Wanderson romao morena L.Sodre Deleon N.Correa Denise B.Rocha Pinto Fabio O.m.Alonso marcos N.Eberlin Institute of Criminalistic Carlos Eboli Rio de JaneiroBrazil National Institute of metrology Standardization and Industrial QualityAv.N.Sra.das GracasDuque de CaxiasBrasil ThomSon mass Spectrometry Laboratory Institute of ChemistryUniversity of CampinasCampinasBrazil

Cocaine and crack cocaine are usually seized with a great diversity of adulterants, such as benzocaine, lidocaine, caffeine, and procaine. The forensic identification of cocaine in these drug mixtures is normally performed using colorimetric testing kits, but these tests may suffer from interferences providing false-positive or false-negatives. In this work, we describe the use of thin layer chromatography coupled to easy sonic-spray ambient ionization mass spectrometry (TLC/EASI-mS) for rapid and secure analysis of cocaine and crack cocaine. Fifteen cocaine samples were analyzed, and all of them revealed positive TLC/EASI-mS results for cocaine, but other drugs and adulterants were also detected such as lidocaine, caffeine, benzocaine, lactose, benzoylecgonine, and ecgonidine. False positives and false negatives, as judged by the TLC Rf values, were identified via on-spot characterization by EASI-mS. The TLC/EASI-mS combination seems therefore to provide an appropriate technique for secure forensic investigations of illicit drugs.

关键词： Cocaine Crack TLC Illicit Drug EASI-mS

Chemical Fingerprinting of Counterfeits of Viagra and Cialis Tablets and Analogues via Electrospray Ionization mass Spectrometry

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American Journal of Analytical Chemistry 2011年第8期2卷 919-928页

作者： Rafael S.Ortiz Kristiane de Cassia mariotti Wanderson romao marcos N.Eberlin Renata P.Limberger Paulo mayorga Rio Grande do Sul Technical and Scientifical Division Brazilian Federal PolicePorto AlegreBrazil Department of Pharmacy Federal University of Rio Grande do SulPorto AlegreBrazil ThomSon mass Spectrometry Laboratory Institute of ChemistryUniversity of Campinas-UNICAMPCampinasBrazil Department of Chemistry Federal University of Espirito SantoVitoriaBrazil

Direct infusion electrospray ionization mass spectrometry (ESI-mS) is shown to provide an efficient tech- nique for the investigation of polar composition of forensic tablets for male erectile dysfunction. ESI-mS fingerprinting of 41 commercial sildenafil samples (Viagra?, Cialis?, Lazar?, Libiden?, , maxfil?, , Plenovit?, Potent 75?, Rigix?, V-50?, Vimax?, Pramil 75? and Pramil?) and 56 counterfeit samples (Viagra and Cialis) were obtained. The spectra for the authentic Viagra? tablets showed abundant ions exclusively corresponding to the sildenafil (SLD) molecule: [SLD + H]+ of m/z 475;[SLD + Na]+ of m/z 497;and [2SLD + H]+ of m/z 949. The spectra for com-mercial sildenafil samples also showed predominat SLD ions. Tablets of authentic Cialis? showed mainly ions of m/z 343, 365 and 707 from the lactose molecule (the excipient);as well as a minor ion of m/z 390 corresponding to the active ingredient tadalafil (TAD) in its protonated form [TAD + H]+. For counterfeit Cialis samples, how-ever, normally TAD ions of much high abundances was observed, together with ions corresponding to sildenafil analogues such as those of m/z 489 (homosildenafil) and 505 (hydroxyhomosildenafil). Principal component analysis was applied to ESI-mS fingerprint data, placing samples according to their contents of active ingredients hence authentic and counterfeit samples are easily recognized.

关键词： ESI-mS Fingerprinting Counterfeiting Viagra^(█) Cialis^(█)

Biomedical Perspectives About Women with Chronic Pelvic Pain:A Qualitative Analysis

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International Journal of Clinical medicine 2012年第5期3卷 411-418页

作者： Paula P.Souza Adriana P.m.S.romao Ana marcia S.Nakano Julio C.Rosa-e-Silva Francisco J.Candido-dos-Reis Antonio A.Nogueira Omero B.Poli-Neto Department of Gynaecology and Obstetrics School of Medicine University HospitalUniversity of Sao Paulo at Ribeirao PretoRibeirao PretoBrazil Department of Public Health School of NursingUniversity of Sao Paulo at Ribeirao PretoRibeirao PretoBrazil School of medicine University Hospital University of Sao Paulo at Ribeirao PretoRibeirao PretoBrazil

Because the aetiology of chronic pelvic pain is complex, studies of the condition involve extensive investigation but provide few conclusions. Numerous studies have addressed the experiences of women with chronic pelvic pain, as well as the interaction between those women and their health care providers. Our objective was to investigate how physicians at a specialised clinic perceive the medical care provided to such women. This was a qualitative study employing semi-structured interviews and content analysis. We interviewed seven physicians at the Chronic Pelvic Pain Outpatient Clinic of the University Hospital, Faculty of medicine of Ribeir?o Preto, University of S?o Paulo, Brazil. medical training and practice constituted the central theme of the study, which was subdivided into categories addressing the influence that the current medical training has on the type of medical care provided to women with chronic pelvic pain. medical practice has been characterised by a reductionist approach to health and illness, as well as by the fragmentation of health care. These characteristics are, to a certain extent, the result of the biomedical model of education, which has been predominant, ignoring social, cultural, psychological and emotional aspects. There is a need to shift the medical paradigms toward a humanistic model of health care. We hope that we have provided a critical view of current medical training and practice, as well as of their effects in various health care settings, particularly in the provision of care to women with chronic pelvic pain.

关键词： Chronic Pelvic Pain Biomedical model Qualitative Research

Coconut Oil Reduces Visceral Adipocyte Size and Improves the metabolic Profile of Rats Fed a High-Carbohydrate Diet

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Journal of Pharmacy and Pharmacology 2019年第3期7卷 98-109页

作者： Valéria Schoffen romao-Carrascoza Rosangela Fernandes Garcia Lorena Lima Garo maria montserrat Diaz Pedrosa Nilton de Almeida Brito Clairce Luzia Salgueiro-Pagadigorria márcia do Nascimento Brito Department of Physiological Sciences State University of MaringáMaringá87020900PRBrazil Department of medicine State University of MaringáMaringá87020900PRBrazil

This work aimed to evaluate the effects of the food supplementation with coconut oil(CO)on biometric and metabolic parameters in an animal model of *** rats fed a high-carbohydrate diet(HCD rats)develop metabolic disorders very similar to those found in human metabolic syndrome,including increased adiposity,insulin resistance(IR),non-alcoholic fatty liver disease(NAFLD)and *** HCD rats had normal fasting glycemia,they developed IR as revealed by Homa-IR index and,after glucose overload,exhibited higher peaks of insulinemia and *** alterations were accompanied by a higher formation of advanced glycated end products(AGEs).The supplementation with CO improved IR,reduced AGEs formation and reverted *** effects could be due to CO action of reducing visceral adipocyte size and take place without undesirable collateral effects on lipid profile,placing CO as an adjuvant in the treatment of the metabolic syndrome.

关键词： Adipocyte hypertrophy metabolic syndrome insulin resistance NAFLD coconut oil