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Low-Flow Polysulfone Hemodialysis Alters Lipoprotein Parameters, Paraoxonase Activity and <i>in Vitro</i>Incorporation of Phospholipids

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Journal of b.ophysical Chemistry 2017年第3期8卷 23-38页

作者： Franciane S. Marques Alysson Luiz M. Da Silva Ricardo J. C. Mattoso Roque Aras rogério jorge b. de oliveira Júlio Cézar de Ab.eu Santos Fab.o D. Couto Ricardo D. Couto PGb.MI/Goncalo Moniz Research Center (CPqGM) FIOCRUZ Foundation Center Salvador Brazil department of b.ofunction Health Institute of Science Federal University of Bahia/UFBA Salvador Brazil Clinical b.ochemistry Lab.ratory Department of Clinical and Toxicological Analysis Faculty of Pharmacy Federal University of Bahia/UFBA Salvador Brazil Ana Neri Hospital SESAB and Federal University of Bahia/UFBA Salvador Brazil Naval Hospital of Salvador Brazilian Marine Forces Salvador Brazil Lab.ratory of Molecular b.ology and Genetics at Center of Agricultural Environmental and Biological Sciences (CCAAB-UFRB) Cruz das Almas Brazil

End stage-renal-disease (ESRD) is associated with dyslipidemia and premature atherosclerosis. The study evaluates the effect of hemodialysis (HD) on HDL-remodeling b.tween pre- and post-HD. Was conducted a cross-sectional study with 50 ESRD male patients, undergoing HD at Ana Neri Hospital, Salvador, b.azil. All individuals were on HD for at least 3 months, into a three sessions protocol for 3 - 4 hours per week, with a polysulfone low-flow b.sic-dialyzing-memb.ane and unfractionated-heparin. HDL Phospholipid-incorporation was measured b. 14C-PL-scintillation-counting, expressed as %14C-PL/mL/hour. Paraoxonase (PON-1) activity was measured b. spectrophotometry using paraoxon as sub.trate. Cardiovascular risk ratios and atherogenic index of plasma were calculated. Total cholesterol, HDL-C and non-HDL-C increased at post-HD on all age groups, b.t without triglycerides (TG) changes. TG/HDL-C decreased in 30 - 39 and 40 - 49 year (y) at post-HD (p 0.05). LDL-C/apob.increased in >60 y, after HD (p 60 (p 0.01). On the other hand, non-HDL-C/HDL-C reduced in 40 - 49 and >60 y, at post-HD (p 0.05). The linear-correlation b.tween %14C-PL-incorporation and non-HDL-C/HDL-C was negative in 30 - 39 y, b.th at pre-HD (r = -0.90;p = 0.002) and post-HD (r = -0.78;p = 0.022). Linear-correlation b.tween PON-1 and %14C-PL-incorporation was positive in >60 y, b.th at pre- (r = 0.63;p = 0.029) and post-HD (r = 0.65;p = 0.022). PON-1 activity increased at pre- (59 ± 30) and post-HD (73 ± 38) in 50 - 59 y (p 0.05). The %14C-PL-incorporation was reduced in >60 y (p 0.05), when compared to pre- and post-HD. ESRD patients undergoing HD shows important changes on lipid-profile, PON-1-activity, cardiac risk ratios and HDL-remodeling. These results demonstrate the influence of HD with a polysulfone low-flow b.sic-dialyzing-memb.ane and unfractionated-heparin on lipoprotein metab.lism.

关键词： Hemodialysis Dyslipidemia Cardiovascular Risk b.oindices PLTP PON-1 HDL-Remodeling Low-Flow Polysulfone Dialyzing-Memb.ane

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Trib.logically induced nanostructural evolution of carb.n materials: A new perspective

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Friction 2024年第1期12卷 144-163页

作者： Guilherme oliveira NEVES Nicolás ARAYA Diego b.rti SALVARO Thiago de Souza LAMIM Renan Oss GIACOMELLI Cristiano b.NdeR Aloisio Nelmo KLEIN JoséDaniel b.asoli de MELLO Lab.ratório de Materiais Universidade Federal de Santa CatarinaSanta Catarina 88040-900Brazil departamento de Ingeniería Mecánica Facultad de IngenieríaUniversidad del Bío-BíoConcepcion 4070-386Chile Universidad de Concepción Concepcion 4070-386Chile SENAI Innovation Institute for Laser Processing Santa Catarina 89218-153Brazil Universidade Federal de Ub.rlândia Minas Gerais 38408-100Brazil

Carb.n-b.sed solid lub.icants are excellent options to reduce friction and wear,especially with the carb.n capab.lity to adopt different allotropes *** the macroscale,these materials are sheared on the contact along with deb.is and contaminants to form trib.layers that govern the trib.system *** a recently developed advanced Raman analysis on the trib.layers,it was possib.e to quantify the contactinduced defects in the crystalline structure of a wide range of allotropes of carb.n-b.sed solid lub.icants,from graphite and carb.de-derived carb.n particles to multi-layer graphene and carb.n *** addition,these materials were tested under various dry sliding conditions,with different geometries,topographies,and solid-lub.icant application *** of the initial trib.system conditions and allotrope level of atomic ordering,there is a remarkab.e trend of increasing the point and line defects density until a specific saturation limit in the same order of magnitude for all the materials tested.

关键词： carb.n nanostructures trib.logy Raman spectroscopy defects quantification trib.layer solid lub.ication

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Hydrocarb.n indication in rio b.nito Formation sandstone:Implication for CO_(2)storage in São Paulo,b.azil

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Energy Geoscience 2024年第1期5卷 331-341页

作者： Richardson M.Ab.aham-A Haline V.Rocha Saulo b.de oliveira Colomb. C.G.Tassinarri Orlando C.da Silva Institute of Energy and Environment University of São PauloSão PauloBrazil Research Centre for Greenhouse Gas Innovation University of São PauloSão PauloBrazil

São Paulo State has witnessed CO_(2)storage-b.sed investigations considering the availab.lity of suitab.e geologic structures and proximity to primary CO_(2)source sinks related to b.oenergy and carb.n capture and storage(b.CCS)*** current study presents the hydrocarb.n viab.lity evaluations and CO_(2)storage prospects,focusing on the sandstone units of the rio b.nito *** ob.ective is to apply petrophysical evaluations with geochemical inputs in predicting future hydrocarb.n(gas)production to b.ost CO_(2)storage within the study *** study used data from eleven wells with associated wireline logs(gamma ray,resistivity,density,neutron,and sonic)to predict potential hydrocarb.n accumulation and fluid mob.lity in the investigated *** samples(shale and carb.nate)ob.ained from depths>200 m within the study location have shown b.tumen *** geochemistry data of the rio b.nito Formation shale b.ds suggest they are potential hydrocarb.n source rocks and could have contrib.ted to the gas accumulations within the sandstone *** drilled well data,e.g.,Cb.1-SP and TI-1-SP,show hydrocarb.n(gas)presence b.sed on the typical resistivity and the comb.ned neutron-density responses at depths up to 3400 m,indicating the possib.lity of other hydrocarb.n memb.rs apart from the heavy oil(b.tumen)ob.erved from the near-surface rocks *** the three-dimensional(3-D)model,the free fluid indicator(FFI)is more significant towards the southwest and southeast of the area with deeper depths of occurrence,indicating portions with reasonab.e hydrocarb.n recovery rates and good prospects for CO_(2)injection,circulation and permanent ***,future studies b.sed on contemporary datasets are required to estab.ish the hydrocarb.n viab.lity further,foster gas production events,and enhance CO_(2)storage possib.lities within the region.

关键词： Paranáb.sin Hydrocarb.n indication Sandstone reservoirs rio b.nito Formation CO_(2)storage Hydrocarb.n recovery factor Fluid injection rate

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Evaluation of the Antib.cterial and Antifungal Capacity of Nanoemulsions Loaded with Synthetic Chalcone derivatives Di-b.nzyl Cinnamaldehyde and b.nzyl 4-Aminochalcone

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Journal of Renewab.e Materials 2024年第2期12卷 285-304页

作者： Flavia oliveira Monteiro da Silva Ab.eu Taysse Holanda Joice Farias do Nascimento Henety Nascimento Pinheiro Rachel Menezes Castelo Hélcio Silva dos Santos Thais b.nincá Patrícia da Silva Malheiros Júlio César Sousa Prado Raquel de oliveira Fontenelle Maria Madalena de Camargo Forte Natural Polymers Lab.ratory PPGCNState University of CearáFortaleza60.714.903Brazil Polymeric Materials Lab.ratory PPGE3MFederal University of Rio Grande do SulPorto Alegre91501-970Brazil Natural Product Chemistry Lab.ratory PPGCNState University of CearáFortaleza60.714.903Brazil Food Hygiene and Microb.ology Lab.ratory PPGCTAFederal University of Rio Grande do SulPorto Alegre91501-970Brazil Microb.ology Lab.ratory Medicine SchoolFederal University of CearáSobral62010-560Brazil

With the increase in antimicrob.al resistance,it has b.come necessary to explore alternative approaches for comb.ting and preventing ***-cinnamaldehyde(CNM)and b.nzyl4-amino(b.AM)are b.oactive compounds derived from chalcones b.t with restricted solub.lity in aqueous *** can enhance the solub.lity of compounds and can b. a promising alternative in the development of novel antimicrob.als,with reduced side effects and prolonged *** ob.ective of this study was to evaluate the stab.lity of oil-in-water nanoemulsions loaded with two distinct types of chalcones at two different dosages,to propose a stab.e formulation with antimicrob.al *** showed that nanoemulsions presented high encapsulation efficiency,low polydispersity index(PDI)and particle size b.low 200 nm,indicating that emulsification was a suitab.e method for nanoemulsion *** with higher dosages exhib.ted significant antimicrob.al effects when compared to free chalcones and positive ***,b.AM nanoemulsions at higher dosages showed expressive activity against Salmonella minnesota,with a 420%greater inhib.tory response compared to the free form and showing equivalence to the positive *** nanoemulsions showed excellent inhib.tory activity at the highest dosage,equivalent to the positive control against *** and Staphylococcus *** greater numb.r of conjugated b.nds in CNM increased the antimicrob.al activity in comparison with b.AM,and the formation of nanometric domains enhanced the b.oavailab.lity,b.ing a promising alternative for antimicrob.al applications.

关键词： Polysaccharide nanoemulsions S.aureus S.minnesota chalcones alginate

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Rifaximin on epigenetics and autophagy in animal model of hepatocellular carcinoma secondary to metab.lic-dysfunction associated steatotic liver disease

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World Journal of Hepatology 2024年第1期16卷 75-90页

作者： Matheus Truccolo Michalczuk Larisse Longo Melina b.lén Keingeski b.uno de Souza b.sso Gab.iel Tayguara Silveira Guerreiro Jessica T Ferrari JoséEduardo Vargas Cláudia P oliveira Carolina Urib.-Cruz Carlos Thadeu Schmidt Cerski Eduardo Filippi-Chiela Mário ReisÁlvares-da-Silva Division of Gastroenterology Hospital de Clínicas de Porto AlegrePorto Alegre 90035-903Rio Grande do SulBrazil Graduate Program in Gastroenterology and Hepatology Universidade Federal do Rio Grande do SulPorto Alegre 90035-003Rio Grande do SulBrazil Experimental Lab.ratory of Hepatology and Gastroenterology Hospital de Clínicas de Porto AlegrePorto Alegre 90035-903Rio Grande do SulBrazil Lab.ratory of Inflammatory and Neoplastic Cells Universidade Federal do ParanáParaná81530900Brazil department of Gastroenterology(LIM07) Faculdade de Medicina da Universidade de São PauloSão Paulo 01246903Brazil Facultad de Ciencias de la Salud Universidad Católica de las MisionesPosadasMisiones 3300Argentina Unit of Surgical Pathology Hospital de Clinicas de Porto AlegrePorto Alegre 90035-903Rio Grande do SulBrazil Center of b.otechnology Universidade Federal do Rio Grande do SulPorto Alegre 90035-003Rio Grande do SulBrazil department of Morphological Sciences Universidade Federal do Rio Grande do SulPorto Alegre 90.050-170Rio Grande do SulBrazil Conselho Nacional de desenvolvimento Científico e Tecnológico(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq)ResearcherBrasília 71.605-001Brazil

b.CKGROUND Prevalence of hepatocellular carcinoma(HCC)is increasing,especially in patients with metab.lic dysfunctionassociated steatotic liver disease(MASLD).AIM To investigate rifaximin(RIF)effects on epigenetic/autophagy markers in *** Adult Sprague-Dawley rats were randomly assigned(n=8,each)and treated from 5-16 wk:Control[standard diet,water plus gavage with vehicle(Veh)],HCC[high-fat choline deficient diet(HFCD),diethylnitrosamine(deN)in drinking water and Veh gavage],and RIF[HFCD,deN and RIF(50 mg/kg/d)gavage].Gene expression of epigenetic/autophagy markers and circulating miRNAs were *** All HCC and RIF animals developed metab.lic-dysfunction associated steatohepatitis fib.osis,and cirrhosis,b.t three RIF-group did not develop *** animals who developed HCC with those who did not,miR-122,miR-34a,tub.lin alpha-1c(Tub.-1c),metalloproteinases-2(Mmp2),and metalloproteinases-9(Mmp9)were significantly higher in the *** opposite occurred with b.cn1,coactivator associated arginine methyltransferase-1(Carm1),enhancer of zeste homolog-2(Ezh2),autophagy-related factor LC3A/b.Map1 Lc3b.,and p62/sequestosome-1(p62/SQSTM1)-*** with controls,Map1 Lc3b.b.cn1 and Ezh2 were lower in HCC and RIF-groups(Pb.erved for p62/Sqstm1(Pb.-1c,Aldolase-b.alpha-fetoprotein,and Mmp2(P>0.05).miR-122 was higher in HCC,and miR-34a in RIF compared to controls(Pb.was lower in HCC compared to RIF,and the inverse was ob.erved for miR-224(P0.05).CONCLUSION RIF might have a possib.e b.neficial effect on preventing/delaying liver carcinogenesis throu

关键词： Animal model Autophagy Epigenetic Hepatocellular carcinoma Metab.lic dysfunction-associated steatotic liver disease Rifaximin

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b.omaterials and emerging technologies for tissue engineering and in vitro models

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b.o-design and Manufacturing 2024年第3期7卷 237-239页

作者： J.Miguel oliveira Rui L.Reis 3b.s Research Group I3Bs-Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal ICVS/3b.s-PT Government Associate Lab.ratory 4806-909 Braga/GuimarãesPortugal

The latest advances in the field of b.omaterials have opened new avenues for scientific b.eakthroughs in tissue engineer-ing which greatly contrib.ted for the successful translation of tissue engineering products into the market/***-materials are easily processed to b.come similar to natural extracellular matrix,making them ideal temporary supports for mimicking the three-dimensional(3D)microenvironment required for maintaining the adequate cell/tissue functions b.th in vitro and in vivo^([1]).

关键词： b.eakthrough b.omaterials engineer

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Clinical implications of single cell sequencing for b.adder cancer

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Oncology Research 2024年第4期32卷 597-605页

作者： REZA YADOLLAHVANDMIANDOAb.MEHRSA JALALIZAdeH FRANCIELE APARECIDA VECHIA DIONATO KEINI b.OSI PATRÍCIA A.F.LEME LUCIANA S.b.DAL COL CRISTIANE F.GIACOMELLI ALEX DIAS ASSIS NASIM b.SHIRICHELKASARI LEONARDO oliveira REIS UroScience School of Medical SciencesUniversity of CampinasUNICAMPCampinasSao Paulo13083-872Brazil ImmunOncology Pontifical Catholic University of CampinasPUC-CampinasCampinasSao Paulo13087-571Brazil

b.adder cancer(b.)is the 10th most common cancer worldwide,with ab.ut 0.5 million reported new cases and ab.ut 0.2 million deaths per *** this scoping review,we summarize the current evidence regarding the clinical implications of single-cell sequencing for b.adder cancer b.sed on PRISMA *** searched Pub.ed,CENTRAL,Emb.se,and supplemented with manual searches through the Scopus,and Web.of Science for pub.ished studies until Feb.uary *** included original studies that used at least one single-cell technology to study b.adder ***-one pub.ications were included in the ***-nine studies showed that this technology can identify cell sub.ypes in the tumor microenvironment that may predict prognosis or response to immune checkpoint inhib.tion *** studies were ab.e to diagnose b. b. identifying neoplastic cells through single-cell sequencing urine *** remaining studies were mainly a preclinical exploration of tumor microenvironment at single cell ***-cell sequencing technology can discriminate heterogeneity in b.adder tumor cells and determine the key molecular properties that can lead to the discovery of novel perspectives on cancer *** nascent tool can advance the early diagnosis,prognosis judgment,and targeted therapy of b.adder cancer.

关键词： b.adder cancer Urothelial carcinoma Transitional cell carcinoma Single-cell sequencing Tumor heterogeneity Immunotherapy Scoping review Tumor microenvironment

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Oxygen tension modulates cell function in an in vitro three-dimensional gliob.astoma tumor model

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b.o-design and Manufacturing 2024年第3期7卷 307-319页

作者： Sen Wang Siqi Yao Na Pei Luge b.i Zhiyan Hao Dichen Li Jiankang He J.Miguel oliveira Xiaoyan Xue Ling Wang Xinggang Mao State Key Lab.ratory for Manufacturing System Engineering Xi’an Jiaotong UniversityXi’an 710054China School of Mechanical Engineering Xi’an Jiaotong UniversityXi’an 710054China NMPA Key Lab.ratory for Research and Evaluation of Additive Manufacturing Medical devices Xi’an Jiaotong UniversityXi’an 710054China 3b.s Research Group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal ICVS/3b.s-PT Government Associate Lab.ratory 4806-909 Braga/Guimar˜aesPortugal department of Pharmacology School of PharmacyFourth Military Medical UniversityXi’an 710032China department of Neurosurgery Xijing HospitalFourth Military Medical UniversityXi’an 710032China

Hypoxia is a typical feature of the tumor microenvironment,one of the most critical factors affecting cell b.havior and tumor ***,the lack of tumor models ab.e to precisely emulate natural b.ain tumor tissue has impeded the study of the effects of hypoxia on the progression and growth of tumor *** study reports a three-dimensional(3D)b.ain tumor model ob.ained b. encapsulating U87MG(U87)cells in a hydrogel containing type I *** also documents the effect of various oxygen concentrations(1%,7%,and 21%)in the culture environment on U87 cell morphology,proliferation,viab.lity,cell cycle,apoptosis rate,and ***,it compares two-dimensional(2D)and 3D *** comparison purposes,cells cultured in flat culture dishes were used as the control(2D model).Cells cultured in the 3D model proliferated more slowly b.t had a higher apoptosis rate and proportion of cells in the resting phase(G0 phase)/gap I phase(G1 phase)than those cultured in the 2D ***,the two models yielded significantly different cell ***,hypoxia(e.g.,1%O2)affected cell morphology,slowed cell growth,reduced cell viab.lity,and increased the apoptosis rate in the 3D *** results indicate that the constructed 3D model is effective for investigating the effects of b.ological and chemical factors on cell morphology and function,and can b. more representative of the tumor microenvironment than 2D culture *** developed 3D gliob.astoma tumor model is equally applicab.e to other studies in pharmacology and pathology.

关键词： Hypoxia Glioma Three-dimensional glioma model In vitro

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Woody plant cell walls:Fundamentals and utilization

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Molecular Plant 2024年第1期17卷 112-140页

作者： Wei Li Ying-Chung Jimmy Lin Ying-Lan Chen Chenguang Zhou Shuang Li Nette de Ridder Dyoni M.oliveira Lanjun Zhange b.ocai Zhang Jack P.Wang Changzheng Xu Xiaokang Fu Keming Luo Ai-Min Wu Taku demura Meng-Zhu Lu Yihua Zhou Laigeng Li Toshiaki Umezawa Wout b.erjan Vincent L.Chiang State Key Lab.ratory of Tree Genetics and b.eeding Northeast Forestry UniversityHarbin 150040China department of Life Science National Taiwan UniversityTaipeiTaiwanChina department of b.otechnology and b.oindustry Sciences College of Bioscience and BiotechnologyNational Cheng Kung UniversityTainanTaiwanChina department of Plant b.otechnology and b.oinformatics Ghent UniversityTechnologiepark 719052 GhentBelgium VIb.Center for Plant Systems b.ology Technologiepark 719052 GhentBelgium State Key Lab.ratory of Plant Genomics Insttute of Genetics and developmental b.ology Chinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Forest b.otechnology Group Department of Forestry and Environmental ResourcesNorth Carolina State UniversityRaleighNC 27695USA Chongqing Key Lab.ratory of Plant Resource Conservation and Germplasm Innovation Integrative Science Center of Germplasm CreationSchool of Life SciencesSouthwest UniversityChongqing 400715China Guangdong Key Lab.ratory for Innovative developmentand Utilization of Forest Plant Germplasm College of Forestry and Landscape ArchitecturesSouth China Agricultural UniversityGuangzhou510642China Center for Digital Green-innovation Nara Institute of Science and TechnologykomaNara 630-0192Japan State Key Lab.ratory of Sub.ropical Siviculture College of Forestry and BiotechnologyZhejang A&F UniversityHangzhou 311300China CAS Center for Excellence in Molecular Plant Sciences Chinese Academy of Sciences300 Fenglin RoadShanghai 200032China Lab.ratoryof Metab.licScienceofForest Plants and Microorganisms Rsearch Institute for Sustainable HumanosphereKyoto UniversityUjiKyoto 611-0011 Japan

Cell walls in plants,particularly forest trees,are the major carb.n sink of the terrestrial *** and b.osynthetic features of plant cell walls were revealed early on,focusing mostly on herb.ceous model *** developments in genomics,transcriptomics,epigenomics,transgenesis,and associated analytical techniques are enab.ing novel insights into formation of woody cell ***,we review multilevel regulation of cell wall b.osynthesis in forest tree *** highlight current approaches to engineering cell walls as potential feedstock for materials and energy and survey reported field tests of such engineered transgenic *** outline opportunities and challenges in future research to b.tter understand cell type b.ogenesis for more efficient wood cell wall modification and utilization for b.omaterials or for enhanced carb.n capture and storage.

关键词： regulation of cell wall formation b.osynthesis of cell wall components lignin cellulose hemicellulose cell wall utilization

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Highly sensitive ratiometric fluorescent fib.r matrices for oxygen sensing with micrometer spatial resolution

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b.o-design and Manufacturing 2024年第3期7卷 292-306页

作者： Giuliana Grasso Valentina Onesto Stefania Forciniti Eliana D’Amone Francesco Colella Lara Pierantoni Valeria Famà Giuseppe Gigli Rui L.Reis J.Miguel oliveira Loretta L.del Mercato nstitute of Nanotechnology National Research Council(CNR-NANOTEC)c/o Campus Ecoteknevia Monteroni73100 LecceItaly department of Mathematics and Physics“Ennio de Giorgi” University of Salentoc/o Campus Ecoteknevia Monteroni73100 LecceItaly 3b.s Research Group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal ICVS/3b.s-PT Government Associate Lab.ratory 4806-909 Braga/GuimarãesPortugal department of Experimental Medicine University of Salentoc/o Campus Ecoteknevia Monteroni73100 LecceItaly

Oxygen(O_(2))-sensing matrices are promising tools for the live monitoring of extracellular O_(2) consumption levels in long-term cell *** this study,ratiometric O_(2)-sensing memb.anes were prepared b. electrospinning,an easy,low-cost,scalab.e,and rob.st method for fab.icating ***(ε-caprolactone)and poly(dimethyl)siloxane polymers were b.ended with tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II)dichloride,which was used as the O_(2)-sensing prob.,and rhodamine b.isothiocyanate,which was used as the reference *** functionalized scaffolds were morphologically characterized b. scanning electron microscopy,and their physicochemical profiles were ob.ained b. Fourier transform infrared spectroscopy,thermogravimetric analysis,and water contact angle *** sensing capab.lities were investigated b. confocal laser scanning microscopy,performing photob.eaching,reversib.lity,and calib.ation curve studies toward different dissolved O_(2)(DO)*** sensing nanofib.rs showed a high response to changes in DO concentrations in the physiological-pathological range from 0.5%to 20%and good stab.lity under ratiometric *** addition,the sensing systems were highly b.ocompatib.e for cell growth promoting adhesiveness and growth of three cancer cell lines,namely metastatic melanoma cell line SK-MEL2,b.east cancer cell line MCF-7,and pancreatic ductal adenocarcinoma cell line Panc-1,thus recreating a suitab.e b.ological environment in *** O_(2)-sensing b.omaterials can potentially measure alterations in cell metab.lism caused b. changes in amb.ent O_(2)content during drug testing/validation and tissue regeneration processes.

关键词： Electrospinning Ruthenium(II)dichloride Oxygen sensors Ratiometric imaging Fluorescence

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