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Effects of Cryofrequency in the Different Swine Skin Tissue Layers: <i>In Vitro</i>and <i>in Vivo</i>Study

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Journal of Biosciences and Medicines 2021年第3期9卷 94-104页

作者： Rafaella Rêgo Maia Patrícia Froes Meyer Josebel da Costa Emerenciano Joseela Joyce Barbosa da silva Júlio davi Costa e silva Liliane Santos de Vasconcellos rodrigo marcel valentim da silva Tarciso Bruno Montenegro Sampaio Ciro dantas Soares Federal Universityof Rio Grande do Norte (UFRN) Natal Brazil University Centre of Rio Grande do Norte (UNI-RN) Natal Brazil Potiguar University (UNP) Natal Brazil Maurício de Nassau College Natal Brazil Universityof Campinas (UNICAMP) S&atilde o Paulo Brazil

Introduction: Cryofrequency is a novel therapy which was developed with the combination of cold (cryotherapy) and heat (radiofrequency) application, causing a thermal shock effect on skin tissue. The thermal shock is believed to alter local metabolism. Objective: To investigate the effect of cryofrequency on the skin tissue of a mini pig in an in vitro and in vivo experimental model. Methodology: A small swine of the S. domesticus species, a male mini-pig (30 kg weight) was elected for the in vivo procedure subject. The in vitro step was performed on the extracted abdominal part of another subject of the same species and breed. Then, after the application of cryofrequency in the monopolar and bipolar form in vivo, a histological analysis was performed. Results: A higher temperature variation was observed comparing the pilot study in vitro with respect to the experimental one in vivo. Microscopically, an increase of collagen fibers and blood supply and decreased adipose tissue was observed after cryofrequency application. Conclusion: With the bipolar cryofrequency, no reduction of adipose tissue was observed, but regarding the connective tissue, extensive areas of collagen deposition were observed, as well as a great amount of new-formed collagen fibers.

关键词： Cryotherapy Radio Frequency Adiposity Collagen Temperature

Effects of Cryofrequence on Localized Adiposity: Case Study

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Journal of Biosciences and Medicines 2022年第1期10卷 16-28页

作者： Patrícia Froes Meyer Laura Vieira Pires Diniz Ruth Gaioso Cartaxo Sousa Júlio davi Costa silva Eneida de Morais Carreiro Rafaella Rêgo Maia Ciro dantas Soares Fernando Cesar Câmara rodrigo marcel valentim da silva University Center of Rio Grande do Norte UNI-RN Natal Brazil Universida de Potiguar (UNP) Natal Brazil Federal University of Rio Grande do Norte (UFRN) Natal Brazil

Cryofrequence is the combination of cryotherapy with radiofrequency, which promotes a “thermal shock” to the tissue, working simultaneously to skin flaccidity and localized adiposity. The efficacy of dermatological and hypodermic cryofrequency treatment was evaluated. This study reports the case of a 45-year-old woman with complaints of flaccidity and fat located in the infra-umbilical region in the preoperative period of abdominoplasty. The evaluation was performed before and after treatment through evaluation protocols, ultrasound examinations and histological findings through optic microscopy, microfocal and immunohistochemical analysis. The treatment was performed in 4 sessions, with a weekly application, using the BHS 156 FULL®—BODY HEALTH BRASIL®apparatus, the parameters were temperature of -10°C, power in 70%, duration of 8 minutes. After the surgical procedure, parts of treated and untreated abdominal tissues were removed for evaluation. In the tests performed, significant destruction of adipocytes was detected, with consequent reduction of fat and greater expression of caspase-3 in the treated tissue, being constated the macrophage increase by CD68 expression. There was improvement in flaccidity, evidenced by the significant increase in the number of fibroblasts and inflammatory cells positive for Ki67, bringing more quantity and better quality to the collagen.

关键词： Cryofrequence Localized Adiposity Skin Flaccidity Abdominoplasty

Comparative Study of Collagen Stimulation Using Semi-Ablative and Non-Ablative Radiofrequency

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Journal of Biosciences and Medicines 2022年第6期10卷 79-89页

作者： Patrícia Froes Meyer rodrigo marcel valentim da silva Eneida De Morais Carreiro Rafaella Rêgo Maia Stephany Luanna Queiroga Farias Ciro dantas Soares Yuri Alexander Afonso Gustavo Felipe Pasqual Silene Bazi Ribeiro Roberto Tiago Alves Pinheiro University Centre of Rio Grande do Norte (UNIRN) Natal Brazil Estácio de Sá University Natal Brazil Federal University of Rio Grande do Norte Natal Brazil Potiguar University (UNP) Natal Brazil Campinas State University (UNICAMP) Campinas Brazil Brazilian Society of Plastic Surgery Brazil FSG University Centre of Serra Gaúcha Caxias do Sul Brazil MARC Institute Miami USA

Objective: Comparison between semi-ablative and non-ablative radiofrequency for collagen stimulation. Method: Three volunteers with scheduled surgical procedures of abdominoplasty were selected. After the evaluation, the abdominal area was divided into three quadrants, with the right region receiving monopolar non-ablative radiofrequency (RF) application. In contrast, the left region received fractionated radiofrequency (FRF) application, using three different needle sizes (0.5, 1.5, and 3.0 mm), and the intermediate area was kept as a control. After 24 hours, surgery was performed, and the skin flaps were collected for histological and immunohistochemical analyses. Results: An increase in total collagen and type I collagen was observed in the treated groups. There was also an increase in the expression of COX-2, CD68 positive cells (macrophages), and lymphocyte markers (CD3, CD20, and NK-CD57). Furthermore, it was seen that only the use of 3.0-mm needles kept the channels open in the superficial tissue after 24 hours. There were no significant differences between the modalities. Conclusion: Non-ablative and sub-ablative radiofrequency have positive and effective results for flaccidity, demonstrating the success in collagen production. Furthermore, this study is the first to present channel opening and permanence time, which are important to optimize the action of drug delivery.

关键词： Collagen Flaccidity Radiofrequency Drug Delivery

Effect of Microfocused Ultrasound on Facial Rejuvenation: Clinical and Histological Evaluation

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Journal of Biosciences and Medicines 2021年第7期9卷 112-125页

作者： Patricia Froes Meyer Michelle Meleck Fabio dos Santos Borges Esteban Fortuny Stephany Luanna Queiroga Farias Felipe Alexander Caldas Afonso Ciro dantas Soares Eneida de Morais Carreiro rodrigo marcel valentim da silva André Luiz Miranda Barbosa Federal University of Rio Grande do Norte (UFRN) Natal Brazil University Centre of Rio Grande do Norte (UNI-RN) Natal Brazil Michelle Meleck Advanced Aesthetics S&atilde o Paulo Brazil Estácio de Sá University Rio de Janeiro Brazil Universidad Finis Terrae Santiago Chile Potiguar University (UNP) Natal Brazil Campinas State University (UNICAMP) Campinas Brazil Fellow da West Virginia University Morgantown USA

Introduction: Micro focused ultrasound (MFU) is formed by mechanical waves emitted in a focused manner, concentrating thermal energy on a certain point or region, promoting a clinical improvement in wrinkles. Objective: evaluate the effects of micro focused ultrasound on facial rejuvenation. Methodology: This is an experimental study. The sample consisted of 30 volunteers, who were evaluated before treatment, 45 and 90 days after its beginning. A single application of the MFU was performed across the facial region. The parameters used were: 10 MHz cartridge with depth of 1.5 mm, 7 MHz with depth of 3 mm, and 4 MHz with focal depth of 4.5 mm;doses ranged from 0.1 to 2.0 J, according to the sensitivity of the volunteers treated, and the application time was of approximately 90 minutes. Clinical photographic analysis and facial symmetry analysis using Dolphin Imaging software were performed, and validated questionnaires. Two volunteers were submitted to blepharoplasty surgery, and one to rhytidoplasty surgery 45 days after the application of the MFU. Samples of the treated skin were taken after the surgical procedures and submitted to histological and immunohistochemical analyses. The volunteers answered a questionnaire to assess adverse reactions and satisfaction. Results: We observed a visible clinical improvement on the images by photography, and an improvement of facial symmetry in the measurements of the paralateronasal projection (p = 0.007), left lateral mandibular (p = 0.01), and right lateral mandibular (p = 0.008). The histological analysis showed an increase in the amount of fibroblasts (p = 0.02), blood vessels (p = 0.0062) and inflammatory cells (p = 0.0036), in addition to the production of type 1 collagen. In the immunohistochemical analysis, we observed the presence of the macrophage marker IHQ: CD68, indicating phagocytosis of adipose tissue and the presence of fibrosis. In the analysis of adverse reactions, only transient hyperemia was observed with t

关键词： Skin Aging Mechanical Waves Sagging Wrinkles

Effects of Hydrolysed Collagen Associated or Not with Physical Agents on Tissue and Muscle Flaccidity—Firmez Method: Case Study

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Open Journal of Applied Sciences 2022年第1期12卷 82-98页

作者： Rafaella Rêgo Maia Patrícia Froes Meyer rodrigo marcel valentim da silva Eneida De Morais Carreiro Thaynar Gonzaga da silva Lígia De Oliveira Revorêdo Stephany Luanna Queiroga Farias Ciro dantas Soares Michelle Cordeiro Sanz Mariana Adélia Girotto Bussade José Dos Santos Bussade Mônica Etrusco Pedrosa Federal University of Rio Grande do Norte Natal Brazil University Centre of Rio Grande do Norte (UNIRN) Natal Brazil Estácio de Sá University Natal Brazil Potiguar University—UNP Natal Brazil Campinas State University (UNICAMP) Campinas Brazil Higher School of Sciences of Santa Casa de Misericórdia Vitória Brazil University of Säo Judas Tadeu S&#228o Paulo Brazil Federal University of Rio de Janeiro Rio de Janeiro Brazil University of Medical Sciences of Minas Gerais Minas Gerais Brazil

The combination of therapies may be the promising future for the treatment of esthetic alterations more efficiently and in less time, in order to obtain an excellent result. Our objective was to evaluate the effectiveness of hydrolysed collagen associated with radiofrequency and electrostimulation in the treatment of flaccidity in the abdominal and gluteal regions. The sample consisted of 6 women, evaluated through evaluation protocols, questionnaires, dynamometry, ultrasound exams, and histological analysis. The volunteers were distributed into three subgroups: hydrolysed collagen group (G-1), radiofrequency + hydrolysed collagen group (G-2) and radiofrequency + hydrolysed collagen + electrostimulation group (G-3). The ultrasound analysis of the abdominal muscles showed an increase in muscle thickness in all groups, but not significant (p > 0.05). The histological analysis showed an evident increase in type I collagen in groups G-2 and G-3, with proliferating cells (Ki67+). The results of dynamometry showed significant values in different times (p days (p = 0.03) and with 60 days (p = 0.04). All participants rated the treatment positively. The use of combined therapies has shown superior results for the treatment of abdominal and gluteal flaccidity when compared with its isolated use, being the combination of therapies important in the treatment of flaccidity.

关键词： Sagging Collagen Radiofrequency Electro-Stimulation

A Parameter-Space Analysis of the Rikitake System

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Chinese Physics Letters 2013年第12期30卷 13-16页

作者： rodrigo A.da silva Paulo C.Rech Departamento de Física Universidade do Estado de Santa Catarina89219-710 JoinvilleBrazil

We investigate analytically and numerically the dynamics of the Rikitake *** Routh–Hurwitz criterion is used to study the stability of the equilibrium points of the differential equation system model,as functions of two *** dynamics of the model are numerically studied using diagrams that associate colors to the largest Lyapunov exponent value,in two-dimensional parameter ***,phase-space plots and bifurcation diagrams are used to distinguish periodic and chaotic attractors.

关键词： bifurcation exponent chaotic

Pathogenesis and clinical management of arboviral diseases

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World Journal of Virology 2025年第1期14卷 9-50页

作者： Victoria Cenci Dietrich Juan Marcos Caram Costa Marina Maria Gomes Leite Oliveira Carlos Eduardo Oliveira Aguiar Luis Guilherme de Oliveira silva marcel silva Luz Fabian Fellipe Bueno Lemos Fabrício Freire de Melo Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil

Arboviral diseases are viral infections transmitted to humans through the bites of arthropods,such as mosquitoes,often causing a variety of pathologies associated with high levels of morbidity and *** the past decades,these infections have proven to be a significant challenge to health systems worldwide,particularly following the considerable geographic expansion of the dengue virus(DENV)and its most recent outbreak in Latin America as well as the difficult-tocontrol outbreaks of yellow fever virus(YFV),chikungunya virus(CHIKV),and Zika virus(ZIKV),leaving behind a substantial portion of the population with complications related to these ***,the world is experiencing a period of intense globalization,which,combined with global warming,directly contributes to wider dissemination of arbovirus vectors across the ***,all continents remain on high alert for potential new ***,this review aims to provide a comprehensive understanding of the pathogenesis of the four main arboviruses today(DENV,ZIKV,YFV,and CHIKV)discussing their viral characteristics,immune responses,and mechanisms of viral evasion,as well as important clinical aspects for patient *** includes associated symptoms,laboratory tests,treatments,existing or developing vaccines and the main associated complications,thus integrating a broad historical,scientific and clinical approach.

关键词： Arboviruses Arbovirus infections Dengue Zika virus Yellow fever Chikungunya virus Clinical diagnosis Pathogenesis Flavivirus Togaviridae infections

A remote sensing approach to estimate the load bearing capacity of soil

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Information Processing in Agriculture 2024年第1期11卷 109-116页

作者： Italo Rômulo Mendes de Souza Edson Eyji Sano Renato Paiva de Lima Anderson rodrigo da silva Statistics and Geoprocessing Laboratory Instituto Federal GoianoGeraldo Silva Nascimento RoadKm 2.575790-000 UrutaíGoiásBrazil Embrapa Cerrados BR-020 RoadKm 18Mailbox 0822373310-970 PlanaltinaDFBrazil Department of Soil Science ‘Luiz de Queiroz’College of AgricultureUniversity of São PauloAv.Pádua Dias235-AgronomiaPiracicabaSP 13418-900Brazil

Preconsolidation pressure(σp)of soil can be considered as an indicator of the Load Bearing Capacity(LBC),which is the tolerated surface pressure before compaction,often caused by the traffic of agricultural *** this pioneering study,a remote sensing approach was introduced to estimate LBC throughσp from soils of the“Rio Preto”Hydrographic Basin,Bahia State,Brazil,in a monthly time lapse from 2016 to ***,σp is measured by a laborious and time demanding laboratory analysis,making it unfeasible to map large *** innovative methodology of this work consists of combining active–passive satellite data on soil moisture and pedotransfer functions of clay content and water matric potential to obtain geo-located estimates ofσ*** were analysed under different classes of soil use,land cover and slope;95%confidence intervals were built for the time series of mean values of LBC for each *** overall seasonal variation in LBC estimates is similar in areas with annual crops,grasslands and natural vegetation,and flat areas are less affected by soil moisture variations over the year(between seasons).LBC decreased,in general,at about 0.5%a year in flat ***,these areas demand attention,since they occupy 86%of the Basin and are mostly subjected to agricultural soil management and surface pressure by heavy machinery.

关键词： Soil compaction SMAP Preconsolidation stress Brazilian Cerrado

Efficiency of soil-applied ^(67)Zn-enriched fertiliser across three consecutive crops

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Pedosphere 2021年第4期31卷 531-537页

作者： Edson M.MATTIELLO Eduardo L.CANCELLIER rodrigo C.da silva Fien DEGRYSE Roslyn BAIRD Mike J.MCLAUGHLIN Department of Soil Science Universidade Federal de ViçosaViçosaMinas Gerais(Brazil) Department of Soil Science Universidade Federal de LavrasMinas Gerais(Brazil) The University of Adelaide School of AgricultureFood and WineFertiliser Technology Research CentrePMB 1Waite CampusGlen Osmond SA 5064(Australia)

A very small amount of applied zinc(Zn) is taken up by crops, resulting in low recovery by plants. Adding elemental sulphur to zinc oxide(ZnO) fertiliser could improve Zn solubilisation and exert a higher residual effect on crops than soluble Zn sources. We produced an isotopically labelled Zn-elemental sulphur fertiliser and evaluated its performance in comparison to traditional Zn sources during sequential crop cultivation. Three ^(67)Zn-labelled fertilisers,ZnO, zinc sulphate(ZnSO_(4)), and ZnO co-granulated with elemental sulphur(ZnOS^(0)), were soil applied, and their contributions to the uptake of Zn by three consecutive crops, wheat, ryegrass, and corn, were assessed in a 294-d pot experiment. The contributions of Zn fertilisers followed the order: ZnSO_(4) > ZnO= ZnOS^(0). The relative contributions of Zn fertilisers were lower in the first crop than in the subsequent crops. The overall recovery of applied Zn by the three crops was higher for ZnSO_(4) than for ZnO and ZnOS^(0), reaching 1.56%, 0.45%, and 0.33% of the applied Zn, respectively. Zinc recovery by plants was very low, regardless of the source of Zn. Adding elemental sulphur to ZnO did not increase its effectiveness up to 294 d after application. Fertiliser contribution was higher for the subsequent crops than for the initial crop, indicating the importance of assessing the residual effects of Zn fertilisers.

关键词： agronomic biofortification elemental sulphur fertilizer residual effect plant uptake Zn bioavailability Zn fertilization Zn source