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Coxiella burnetii:living inside the host cell

BIOCELL 2019年第1期43卷 1-6页

作者： Jesus Sebastian DISTEL Walter BERON rodolfo matias ortiz flores Instituto de Histologia y Embriologia de Mendoza(IHEM) ONICETUniversidad Nacional de CuyoMendozaArgentina Facultad de Odontologia Universidad Nacional de CuyoMendozaArgentina

Coxiella burnetii is an obligate intracellular pathogen and the causative agent of Q *** this brief review,we describe how recently described mechanisms help our understanding of *** invasion and its survival in the host cell by the formation of a replicative niche:the Coxiella-containing *** describe the actin-associated proteins involved in the internalization of ***,and we discuss the contribution of diverse degradation pathways of the cell during the formation and stabilization of the Coxiella-containing vacuole.

关键词： Actin Coxiellosis Phagocytic pathway Q fever

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Biotechnological Alternatives for the Utilization of Dairy Industry Waste Products

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Advances in Bioscience and Biotechnology 2015年第3期6卷 223-235页

作者： Cortés-Sánchez Alejandro De Jesus Valle-González Elba Ruth Salazar-flores rodolfo Daniel Ashutosh Sharma Comisión Federal para la Protección contra Riesgos Sanitarios Departamento de Microbiología Tlalpan Mexico Escuela de ingeniería en alimentos biotecnología y agronomía Instituto Tecnológico y de Estudios Superiores de Monterrey San Pablo Mexico

The industrial obtainment and manufacturing of food cause high levels of pollution because of the generation of waste byproducts. Over the past few years, there has been a significantly increased interest in preserving, restoring and establishing an ecological balance during food production. Many investigators propose biotechnological solutions to the treatment of industrial wastes, especially waste from the dairy industry. The aim of this review is to present biotechnological approaches to the treatment and utilization of wastes from the dairy industry, specifically for milk whey and also discussed biotechnological methods to reduce environmental pollution and obtain chemical compounds with potential applications in the industry.

关键词： Bioactive Peptides Milk Whey Organic Acids Biofuel Biosurfactants

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Detection of <i>Para</i>-Chloroaniline Resulting from the Interaction between Sodium Hypochlorite and Chlorhexidine Analyzed by Mass Spectrometry

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American Journal of Analytical Chemistry 2019年第12期10卷 629-640页

作者： Miriam Muñ oz Arratia Marí a de Lourdes Lanzagorta Rebollo Francisco Javier Pé rez flores rodolfo Garcí a Contreras José Luis Molinari Soriano Ana Marí a Ferná ndez Presas Instituto de Estudios Avanzados en Odontología “Yury Kutler” Mexico City Mexico Instituto de Química Universidad Nacional Autónoma de México Mexico City Mexico Departamento de Microbiología y Parasitología Facultad de Medicina Universidad Nacional Autónoma de México Mexico City Mexico Departamento de Bioquímica y Biología Estructural Instituto de Fisiología Celular Universidad Nacional Autónoma de México México City Mexico

5.1% of sodium hypochlorite (NaOCl) mixed with 2% of chlorhexidine (CHX) forms a brown precipitate that corresponds to para-chloroaniline (PCA), whether PCA is formed after the combination of NaOCl, CHX, and ethylenediaminetetraacetic acid (EDTA) by means of electron impact (high resolution and interlaced scanning) and ionization by fast atom bombardment (FAB), was analyzed. The brown precipitate, showed signals 127 and 153 Da, corresponding to p-chloroaniline and p-chlorophenyl isocyanate, respectively. These results were analyzed and compared with signals from the interlaced scanning program and confirmed with high resolution mass spectrometry analysis and compared with the NIST database. The mass spectra of this precipitated after different days confirmed the evolution of byproducts with the presence of a peak m/z = 127, due to the decrease of the fragment m/z = 153, which disappeared after 180 days. A blue and a white precipitate were observed by the addition of CHX (2%) with or without polyethylene glycol, respectively, EDTA (17%) and NaOCl (5.1%) precipitates contain chlorhexidine (m/z = 505), but no PCA was detected. We confirmed that PCA is not formed directly as a byproduct of CHX oxidation, but through the formation of the para-chlorophenyl isocyanate intermediate, which degrades slowly to PCA.

关键词： Chlorhexidine Para-Chloroaniline Para-Chlorophenyl Isocyanate EDTA Sodium Hypochlorite

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Evaluation of Changes in Actin Filaments of RK13 Cells Infected with <i>Malassezia pachydermatis</i>

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Open Journal of Veterinary Medicine 2018年第2期8卷 15-24页

作者： Iris del Socorro flores Rodríguez Tonatiuh Alejandro Cruz Sánchez José Luis Nieto Bordes Francisco rodolfo González Díaz Carlos Ignacio Soto Zárate Carlos Gerardo García Tovar Laboratorio 6 de Bioprospección Microbiológica Unidad de Investigación Multidisciplinaria (UIM) Facultad de Estudios Superiores Cuautitlán UNAM Cuautitlán Izcallí México Laboratorio 4 de Morfología Veterinaria y Biología Celular Unidad de Investigación Multidisciplinaria (UIM) Facultad de Estudios Superiores Cuautitlán UNAM Cuautitlán Izcallí México

Background: Malassezia pachydermatis is the main causative agent of canine otitis and also of a myriad of dermatological problems in companion animals;its interaction mechanisms with host cells are still unclear. Objectives: To establish an in vitro infection model of M. pachydermatis-exposed RK13 cells in order to evaluate cell morphological changes as well as changes in the structure of actin filaments. Methods: Cultures of RK13 cells were infected with M. pachydermatis, alterations caused by the yeast were evaluated by optical and fluorescence microscopy. Results: M. pachydermatis adheres itself to the cell and produces the formation of multiple agglomerates that cause changes in cell morphology, formation of cell aggregates in overlays, presence of syncytia and destruction of cell culture structure. The damaged cells presented changes in the actin filaments consisting of thickening of the cell cortex and loss of stress fibers. On the other hand, the formation of perinuclear actin rings in the yeasts was observed. Conclusions: An in vitro infection model was established with M. pachydermatis and alterations in cell morphology were observed consisting of changes in the structure of the actin filaments, overgrowth of the cells and the presence of syncytia.

关键词： Malassezia pachydermatis RK13 Cells Actin Filaments

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