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Utilization of RNA sequencing to investigate olfactory dysfunction in chronic rhinosinusitis without nasal polyps:A pilot study

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World journal of Otorhinolaryngology-Head and Neck Surgery 2024年第1期10卷 29-36页

作者： jorge A.Gutierrez III jeremy L.Barth rodney j.schlosser Thomas S.Edwards Timothy L.Smith Zachary M.Soler Department of Otolaryngology-Head and Neck Surgery Medical University of South CarolinaCharlestonSouth CarolinaUSA Department of Regenerative Medicine&Cell Biology Medical University of South CarolinaCharlestonSouth CarolinaUSA Department of Otolaryngology-Head and Neck Surgery Oregon Health&Science UniversityPortlandOregonUSA

Objectives:Prior research on olfactory dysfunction in chronic rhinosinusitis(CRS)has focused on patients with polyps and suggests that direct inflammation of the olfactory cleft mucosa plays a contributory *** purpose of this study was to evaluate gene expression in superior turbinate mucosal specimens,comparing normosmic and dysosmic CRS patients without polyps(CRSsNP).Methods:Tissue samples were obtained from the superior turbinates of patients with CRSsNP at the time of endoscopic sinus *** subsequently underwent RNA sequencing and functional analysis to investigate biological pathways associated with differentially expressed genes between dysosmic(n=7)and normosmic(n=4)***:Differential gene expression analysis comparing dysosmic and normosmic CRSsNP patients showed upregulation of 563 genes and downregulation of 327 *** stringent criteria for multiple comparisons,one upregulated gene(Immediate Early Response 3[IER3])had an false discovery rate(FDR)correction adjustedP value considered statistically significant(P<0.001,fold change 2.69).Reactome functional analysis revealed eight biological pathways significantly different between dysosmic and normosmic patients(P<0.05,FDR correction)including IL-4 and IL-13 signaling,IL-10 signaling,and rhodopsin-like ***:RNA sequencing of the superior turbinates in patients with CRSsNP can provide valuable information regarding biological pathways and genes involved in olfactory *** study supports literature suggesting that Type 2 inflammation may play a role in olfactory dysfunction in at least some patients with *** study also prompts questions regarding the role of IL-10,rhodopsin-like receptors,andIER3 in the pathogenesis of olfactory dysfunction.

关键词： chronic rhinosinusitis without nasal polyps olfactory dysfunction RNA sequencing

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Comparison of trigeminal lateralization with differing stimulants

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World journal of Otorhinolaryngology-Head and Neck Surgery 2024年第1期10卷 66-69页

作者： Tiffany Chen Nicolas S.Poupore Michael C.Shih Thomas S.Edwards Shaun A.Nguyen Zachary M.Soler rodney j.schlosser Department of Otolaryngology‐Head and Neck Surgery Medical University of South CarolinaCharlestonSouth CarolinaUSA

Trigeminal-specific stimulants have been shown to activate different receptors preferentially and this likely accounts for variation in sensory *** is unclear whether trigeminal sensitivity is similar across different transient receptor potential(TRP)receptors or if dysfunction of different receptors results in differing patient ***,a prospective cohort study was conducted,consisting of trigeminal lateralization testing with three different stimulants(eucalyptol,isothiocyanate,acetic acid),olfaction testing with Sniffin'Sticks,and measurement of various patient‐reported outcome measures(PROMs).A total of 50 participants were enrolled across the olfactory *** TDI score was 27.1±8.3(range 7.0–39.5)with 38%normosmic and 62%*** trigeminal lateralization scores out of 20 in the overall cohort were 16.18(2.78)for eucalyptol,14.94(3.49)for mustard oil,and 15.28(3.68)for *** showed a significant correlation with threshold scores of Sniffin'Sticks.A significant correlation was found between acetic acid and various *** of the lateralization scores of the trigeminal stimulants correlated to each other significantly and there was no correlation to *** lack of correlation suggests that the measured sensitivity of one type of TRP receptor may not translate to similar sensitivity of the other *** investigations with TRPV1 and TRPA1 agonists are needed to corroborate our findings.

关键词： chemosensory olfaction trigeminal TRP receptor

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Identification of microbial contaminants in sinus rinse squeeze bottles used by allergic rhinitis patients

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World journal of Otorhinolaryngology-Head and Neck Surgery 2019年第1期5卷 26-29页

作者： Shaun A.Nguyen Marc P.Camilon rodney j.schlosser Medical University of South Carolina Department of OtolaryngologyHead&Neck SurgeryCharlestonSCUSA Medical University of South Carolina Department of OtolaryngologyHead&Neck SurgeryCharlestonSCUSARalph H.Johnson VA Medical CenterCharlestonSCUSA

Objective:To identify whether irrigation devices become contaminated when used by patients with allergic rhinitis (AR).Methods:Ten AR patients with no clinical or endoscopic evidence of active sinonasal infection were given a sinus rinse system and instructed on its proper use,cleaning,and *** squeeze bottles (bottle A and bottle B) were given to each patient for twice-a-day *** A was used in the morning and analyzed after four *** B was used in the evening and analyzed after 8 weeks of *** contaminants were cultured from the nose pieces and the inner surface of the bottles obtained from ***:Seventeen sinus rinse devices (17/20) from all individuals in this study grew bacteria commonly in the ***-four bacterial isolates consisting of 14 different species were cultured and identified with most common organisms being bacilli and *** addition,no correlation was apparent between the length of bottle use and the degree of contamination (r =0.13,p =0.76).During the study period,no patient developed acute sinus ***:Microbial contamination of the sinus rinse system occurs commonly,even in uninfected AR patients;however no evidence exists linking this to clinically relevant sinus infections.

关键词： Allergic rhinitis Microbial contaminants Sinus irrigation Sinus rinse Squeeze bottles

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Is SARS-CoV-2 (COVID-19) postviral olfactory dysfunction (PVOD) different from other PVOD?

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World journal of Otorhinolaryngology-Head and Neck Surgery 2020年第S1期6卷 S26-S32页

作者： Sarah A.Imam Wilson P.Lao Priyanka Reddy Shaun A.Nguyen rodney j.schlosser Department of Health and Human Performance The CitadelCharlestonSCUSA Loma Linda University School of Medicine Loma LindaCAUSA Department of Otolaryngology-Head and Neck Surgery Medical University of South CarolinaCharlestonSCUSA

Background:The SARS-CoV-2 virus continues to spread rapidly across the globe af-flicting many with Coronavirus Disease 2019(COVID-19).As the infection rates rise,a growing number of SARS-CoV-2 positive individuals have been reported to complain of olfactory distur-bances at an alarming *** olfactory dysfunction(PVOD)is a well-known phenome-non that may explain the olfactory dysfunction reported by SARS-CoV-2 infected ***:A scoping literature review was performed to identify studies that investigated the mechanisms of postviral olfactory *** demonstrating pathophysiological,his-tological,immunochemical,and epidemiological outcomes of PVOD were ***:Fourteen studies were included in addition to one international news *** studies reported destruction of the olfactory epithelium following intranasal inoculation of various viral strains in *** studies isolated pathogenic,anosmia inciting viruses(Para-influenza virus,Human Coronavirus,Rhinovirus)through nucleic acid *** studies demonstrated female predilection in patients with PVOD and COVID-19 associated ol-factory dysfunction,of which the majority were over 50 years ***:PVOD and COVID-19 associated olfactory dysfunction demonstrates considerable similarities in epidemiological trends and disease sequela of other viruses to suggest identical pathophysiological *** studies such as intranasal inoculation and histological biopsies are needed to support our hypothesis.

关键词： Olfaction Olfactory disorders UPSIT SARS-CoV-2(COVID-19) Postviral olfactory dysfunction

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Cytokines from the pig conceptus:roles in conceptus development in pigs

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journal of Animal Science and Biotechnology 2015年第2期6卷 130-141页

作者： rodney D Geisert Matthew C Lucy jeffrey j Whyte jason W Ross Daniel j Mathew Animal Sciences Research Center University of Missouri Department of Animal Science Iowa State University

Establishment of pregnancy in pigs involves maintaining progesterone secretion from the corpora lutea in addition to regulating a sensitive interplay between the maternal immune system and attachment of the rapidly expanding trophoblast for nutrient absorption. The peri-implantation period of rapid trophoblastic elongation followed by attachment to the maternal uterine endometrium is critical for establishing a sufficient placental-uterine interface for subsequent nutrient transport for fetal survival to term, but is also marked by the required conceptus release of factors involved with stimulating uterine secretion of histotroph and modulation of the maternal immune system. Many endometrial genes activated by the conceptus secretory factors stimulate a tightly controlled proinflammatory response within the uterus. A number of the cytokines released by the elongating conceptuses stimulate inducible transcription factors such as nuclear factor kappa B （NFKB） potentially regulating the maternal uterine proinflammatory and immune response. This review will establish the current knowledge for the role of conceptus cytokine production and release in early development and establishment of pregnancy in the pig.

关键词： Cytokines Embryo development Porcine conceptus Pregnancy Prostaglandins uterus

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Synthesis of Isotopically-Labeled Graphite Films by Cold-Wall Chemical Vapor Deposition and Electronic Properties of Graphene Obtained from Such Films

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Nano Research 2009年第11期2卷 851-856页

作者： Weiwei Cai Richard D.Piner Yanwu Zhu Xuesong Li Zhenbing Tan Herman Carlo Floresca Changli Yang Li Lu M.j.Kim rodney S.Ruoff Department of Mechanical Engineering and the Texas Materials Institute University of Texas at AustinAustinTX 78712USA Institute of Physics Chinese Academy of SciencesBeijing 100190China Department of Materials Science and Engineering University of Texas at DallasRichardsonTX 75083USA

We report the synthesis of isotopically-labeled graphite films on nickel substrates by using cold-wall chemical vapor deposition(CVD).During the synthesis,carbon from^(12)C-and^(13)C-methane was deposited on,and dissolved in,a nickel foil at high temperature,and a uniform graphite film was segregated from the nickel surface by cooling the sample to room *** and transmission electron microscopy,micro-Raman spectroscopy,and X-ray diffraction prove the presence of a graphite *** graphene films obtained from such isotopically-labeled graphite films by mechanical methods have electron mobility values greater than 5000 cm^(2)·V^(-1)·s^(-1)at low ***,such films exhibit the half-integer quantum Hall effect over a wide temperature range from 2 K to 200 K,implying that the graphite grown by this cold-wall CVD approach has a quality as high as highly oriented pyrolytic graphite(HOPG).The results from transport measurements indicate that^(13)C-labeling does not significantly affect the electrical transport properties of graphene.

关键词： Chemical vapor deposition(CVD) isotopically-labeled graphite graphene

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Ultrathin monolithic 3D printed optical coherence tomography endoscopy for preclinical and clinical use

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Light(Science & Applications) 2020年第1期9卷 753-762页

作者： jiawen Li Simon Thiele Bryden C.Quirk rodney W.Kirk johan W.Verjans Emma Akers Christina A.Bursill Stephen j.Nicholls Alois M.Herkommer Harald Giessen Robert A.McLaughlin Australian Research Council Centre of Excellence for Nanoscale BioPhotonics Adelaide Medical SchoolUniversity of AdelaideAdelaideSA 5005Australia Institute for Photonics and Advanced Sensing The University of AdelaideAdelaideSA 5005Australia Institute of Applied Optics(ITO)and Research Center SCoPE University of Stuttgart70569 StuttgartGermany South Australian Health and Medical Research Institute(SAHMRI) AdelaideSA 5000Australia Royal Adelaide Hospital AdelaideSA 5000Australia Monash Cardiovascular Research Centre Monash UniversityMelbourneVIC 3168Australia th Physics Institute and Research Center SCoPE University of Stuttgart70569 StuttgartGermany

Preclinical and clinical diagnostics increasingly rely on techniques to visualize internal organs at high resolution via *** endoscopic probes are necessary for imaging small luminal or delicate organs without causing trauma to ***,current fabrication methods limit the imaging performance of highly miniaturized probes,restricting their widespread *** overcome this limitation,we developed a novel ultrathin probe fabrication technique that utilizes 3D microprinting to reliably create side-facing freeform micro-optics(<130μm diameter)on single-mode *** this technique,we built a fully functional ultrathin aberration-corrected optical coherence tomography *** is the smallest freeform 3D imaging probe yet reported,with a diameter of 0.457 mm,including the catheter *** demonstrated image quality and mechanical flexibility by imaging atherosclerotic human and mouse *** ability to provide microstructural information with the smallest optical coherence tomography catheter opens a gateway for novel minimally invasive applications in disease.

关键词： smallest utilize overcome

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Gene Expression Profiling of Human Myeloid Leukemic MV4-11 Cells Treated with 5-Aza-2’-deoxycytidine

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journal of Cancer Therapy 2012年第3期3卷 177-182页

作者： Kyu-Tae Kim David Mossman Donald Small rodney j. Scott Division of Genetics Hunter Area Pathology Service John Hunter Hospital New Lambton Australia School of Biomedical Sciences and Pharmacy Faculty of Health University of Newcastle Callaghan Australia School of Medicine Johns Hopkins University Baltimore USA

The pyrimidine analog, 5-aza-2’-deoxycytidine (5-aza-dC) is a DNA methyltransferase inhibitor that triggers DNA demethylation leading to the reactivation of epigenetically silenced tumor suppressor genes. To understand the shift in gene expression which mediates the beneficial 5-aza-dC effects in leukemia, we have treated human myeloid derived leukemic cells with 5-aza-dC. Target genes were identified first in MV4-11 cells using a genome-wide gene expression profiling assay to detect differences in treated and untreated cells. From this analysis six genes were identified (HOXA4, HOXD4, HOXA8, HOXD12, CD9 and RGS2) as being significantly different expressed after treatment. To validate microarray data, we performed quantitative PCR on these genes from multiple leukemic cells. The results suggest that these genes are epigenetically regulated indicating that dysregulation of HOXA4, HOXD4, HOXA8, HOXD12, CD9 and RGS2 expression may play an important role in establishing the malignant phenotype in AML.

关键词： 5-Aza-2’-deoxycytidine Gene Expression Profile HOX CD9 RGS2

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Draft Genome Sequence of Mentha Iongifolia and Development of Resources for Mint Cultivar Improvement

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Molecular Plant 2017年第2期10卷 323-339页

作者： Kelly j. Vining Sean R. johnson Amirhossein Ahkami Iris Lange Amber N. Parrish Susan C. Trapp rodney B. Croteau Shannon C.K. Straub Iovanna Pandelova B. Markus Lange Department of Horticulture Oregon State University Corvallis OR 97331 USA M. j. Murdock Metabolomics Laboratory Institute of Biological Chemistry Washington State University Pullman WA 99164-6340 USA Department of Biology Hobart and William Smith Colleges Geneva NY 14456 USA Present address: Pacific Northwest National Laboratory Environmental Molecular Sciences Laboratory (EMSL) Richiand WA 99354 USA Present address: Medifluidics Inc. Fort Collins. CO 80525 USA

The genus Mentha encompasses mint species cultivated for their essential oils, which are formulated into a vast array of consumer products. Desirable oil characteristics and resistance to the fungal disease Verti- cillium wilt are top priorities for the mint industry. However, cultivated mints have complex polyploid ge- homes and are sterile. Breeding efforts, therefore, require the development of genomic resources for fertile mint species. Here, we present draft de novo genome and plastome assemblies for a wilt-resistant South African accession of Mentha Iongifolia （L.） Huds., a diploid species ancestral to cultivated peppermint and spearmint. The 353 Mb genome contains 35 597 predicted protein-coding genes, including 292 disease resistance gene homologs, and nine genes determining essential oil characteristics. A genetic linkage map ordered 1397 genome scaffolds on 12 pseudochromosomes. More than two million simple sequence repeats were identified, which will facilitate molecular marker development. The M. Iongifolia genome is a valuable resource for both metabolic engineering and molecular breeding. This is exemplified by employing the genome sequence to clone and functionally characterize the promoters in a peppermint cultivar, and demonstrating the utility of a glandular trichome-specific promoter to increase expression of a biosynthetic gene, thereby modulating essential oil composition.

关键词： aromatic plant essential oil genome mint Verticillium wilt

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Overcoming Barriers to Uptake of Voluntary Medical Male Circumcision in a Traditionally Circumcising Community in Machinga District, Malawi

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World journal of AIDS 2017年第1期7卷 40-58页

作者： rodney j. Masese jane L. Chimango Noel D. Mbirimtengerenji Community and Mental Health Department Kamuzu College of Nursing University of Malawi Lilongwe Malawi Medical-Surgical Nursing Department Kamuzu College of Nursing University of Malawi Lilongwe Malawi

Introduction: Voluntary medical male circumcision (VMMC) reduces chances of contracting HIV during heterosexual intercourse in males, and risk of cervical cancer in their female sexual partners. However, its uptake among traditionally circumcising communities, where male circumcision carries a cultural significance, has not been studied. Previous research has focused on barriers to uptake of VMMC in non-circumcising communities. This study was conducted to determine socio-cultural barriers to VMMC uptake in order to identify culture-sensitive and evidence-based interventions to increase its uptake. Methodology: This exploratory mixed methods cross-sectional study generated quantitative data from 262 randomly sampled sexually active men;and qualitative data through four focus-group discussions with 58 purposely selected male participants and 10 key informants. Results: The study established that only 6.3% (n = 15) of those circumcised (90.8%, n = 238) were circumcised in clinical setting. Limited access to VMMC services, cost, pain, being attended to by female providers and cultural influences were the major hindrances to uptake of VMMC. Conclusion: Although there is a paradigm shift of preference from traditional male circumcision to VMMC in this community, its uptake is low. The study recommends that besides introducing mobile VMMC services, the Ministry of Health should collaborate with traditional circumcisers and local leaders to provide gender-and-culture-sensitive safer medical male circumcision services.

关键词： VMMC Medical Circumcision Traditional Circumcision Malawi Uptake

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