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Interplays of liver fibrosis-associated microRNAs:Molecular mechanisms and implications in diagnosis and therapy

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Genes & Diseases 2023年第4期10卷 1457-1469页

作者： Hong li Tingli liu Yongchun Yang William C.Cho robin J.Flynn Majid Fasihi Harandi Houhui Song Xuenong luo Yadong Zheng State Key laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhouGansu 730046China Key laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province Zhejiang Provincial Engineering Laboratory for Animal Health Inspection&Internet TechnologyZhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health ManagementChina-Australia Joint Laboratory for Animal Health Big Data AnalyticsCollege of Animal Science and Technology&College of Veterinary Medicine of Zhejiang A&F UniversityHangzhouZhejiang 311300China Department of Clinical Oncology Queen Elizabeth HospitalHong Kong SAR 999077China Department of Infection Biology Institute of Infection and Global HealthUniversity of LiverpoolLiverpool L35RFUK Graduate Studies Office Department of ResearchInnovation and Graduate StudiesWaterford Institute of TechnologyX91 K0EKIreland Research Center for Hydatid Disease in Iran Kerman University of Medical SciencesKerman ***Iran

microRNAs(miRNAs)are a class of non-coding functional small RNA composed of 21e23 nucleotides,having multiple associations with liver ***-associated miRNAs are roughly classified into pro-fibrosis or anti-fibrosis *** former is capable of activating hepatic stellate cells(HSCs)by modulating pro-fibrotic signaling pathways,mainly including TGF-b/SMAD,WNT/b-catenin,and Hedgehog;the latter is responsible for maintenance of the quiescent phenotype of normal HSCs,phenotypic reversion of activated HSCs(aHSCs),inhibition of HSCs proliferation and suppression of the extracellular matrix-associated gene ***,several miRNAs are involved in regulation of liver fibrosis via alternative mechanisms,such as interacting between hepatocytes and other liver cells via exosomes and increasing autophagy of ***,understanding the role of these miRNAs may provide new avenues for the development of novel interventions against hepatic fibrosis.

关键词： Diagnosis Hepatic stellate cell liver fibrosis miRNA Therapy

Association of Varroa destructor females in multiply infested cells of the honeybee Apis mellifera

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Insect Science 2019年第1期26卷 128-134页

作者： Alexis l.Beaurepaire James D.Ellis Klemens J.Krieger robin F.A.Moritz Institut for Biologie Martin-Luther-University Halle-WittenbergHalle a.d.SaaleGermany UR 406 Abeilles et Environnement INRACentre de Recherche Provence-Alpes-Cote d'AzurAvignonFrance Entomology &Nematology Department University Florida GainesvilleFloridaUSA Bayer Animal Health GmbH LeverkusenGermany Department of Zoology and Entomology University of PretoriaPretoriaSouth Africa

The genetic diversity of Varroa destructor (Anderson &Trueman)is limited outside its natural range due to population bottlenecks and its propensity to *** light of the arms race between *** and its honeybee (Apis mellifera l.)host, any mechanism enhancing population admixture of the mite may be *** way that admixture can occur is when two genetically dissimilar mites coinvade a brood cell, with the progeny of the foundresses *** determined the relatedness of 393 pairs of *** foundresses,each pair collected from a single bee brood cell (n =five colonies).We used six microsatellites to identify the genotypes of mites coinvading a cell and calculated the frequency of pairs with different or the same *** found no deviation from random coinvasion,but the frequency of cells infested by mites with different genotypes was *** rate of recombination,coupled with a high transmission rate of mites,homogenized the allelic pool of mites within the apiary.

关键词： Apis mellifera host-parasite coevolution inbreeding avoidance mating behavior population genetics Varroa destructor

Restoration of Mitochondrial Structure and Function within Helicobacter pylori VacA Intoxicated Cells

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Advances in Microbiology 2023年第8期13卷 399-419页

作者： robin l. Holland Kristopher D. Bosi Ami Y. Seeger Steven R. Blanke Department of Pathobiology College of Veterinary Medicine University of Illinois Urbana-Champaign Urbana IL USA Department of Microbiology School of Molecular and Cellular Biology University of Illinois Urbana-Champaign Urbana IL USA Biomedical and Translational Sciences Department Carle Illinois College of Medicine University of Illinois Urbana IL USA

The Helicobacter pylori vacuolating cytotoxin (VacA) is an intracellular, mitochondrial-targeting exotoxin that rapidly causes mitochondrial dysfunction and fragmentation. Although VacA targeting of mitochondria has been reported to alter overall cellular metabolism, there is little known about the consequences of extended exposure to the toxin. Here, we describe studies to address this gap in knowledge, which have revealed that mitochondrial dysfunction and fragmentation are followed by a time-dependent recovery of mitochondrial structure, mitochondrial transmembrane potential, and cellular ATP levels. Cells exposed to VacA also initially demonstrated a reduction in oxidative phosphorylation, as well as increase in compensatory aerobic glycolysis. These metabolic alterations were reversed in cells with limited toxin exposure, congruent with the recovery of mitochondrial transmembrane potential and the absence of cytochrome c release from the mitochondria. Taken together, these results are consistent with a model that mitochondrial structure and function are restored in VacA-intoxicated cells.

关键词： Helicobacter pylori VacA Vacuolating Cytotoxin Mitochondria Mitochondrial Dysfunction Mitochondrial Fission Mitochondrial Transmembrane Potential ATP Mitochondrial Dynamics Oxidative Phosphorylation

New Breakdown Electric Field Calculation for SF6 High Voltage Circuit Breaker Applications

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Plasma Science and Technology 2007年第6期9卷 690-694页

作者： Ph.robin-JOUAN M.YOUSFI AREVA T & D High Voltage Switchgear Research Centre 130 rue L.BLUM 69611 VilleurbanneFrance University of Toulouse LAPLACE-UMR 5213 CNRSUPS118 Route de Narbonne31062 Toulouse Cedex 9France

The critical electric fields of hot SF6 are calculated including both electron and ion kinetics in wide ranges of temperature and pressure, namely from 300 K up to 4000 K and 2 atmospheres up to 32 atmospheres respectively. Based on solving a multi-term electron Boltz- mann equation the calculations use improved electron-gas collision cross sections for twelve SF6 dissociation products with a particular emphasis on the electron-vibrating molecule interactions. The ion kinetics is also considered and its role on the critical field becomes non negligible as the temperature is above 2000 K. These critical fields are then used in hydrodynamics simulations which correctly predict the circuit breaker behaviours observed in the case of breaking tests.

关键词： breakdown electric field hot dissociated SF6 high voltage gas-circuit-breaker

Spatial separation of phosphatase and kinase activity within the Bub complex is required for proper mitosis

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Journal of Molecular Cell Biology 2022年第11期14卷 24-35页

作者： lei Wang Thomas kruse Blanca lópez-Méndez Yuqing Zhang Chunlin Song lei Zhu Bing li Jing Fang Zhimin lu Jakob Nilsson Gang Zhang The Cancer Institute The Affiliated Hospital of Qingdao UniversityQingdao UniversityQingdao 266061China Novo Nordisk Foundation Center for Protein Research Faculty of Health and Medical SciencesUniversity of CopenhagenCopenhagen DK-2200Denmark The Department of Genetics and Cell Biology Basic Medical CollegeQingdao UniversityQingdao 266061China Institute of Translational Medicine Zhejiang University School of MedicineHangzhou 310029China

The Bub1 and BubR1 kinetochore proteins support proper chromosome segregation and mitotic checkpoint activity. Bub1 and BubR1 are paralogs with Bub1 being a kinase, while BubR1 localizes the PP2A-B56 protein phosphatase to kinetochores in humans. Whether this spatial separation of kinase and phosphatase activity is important is unclear as some organisms integrate both activities into one Bub protein. Here, we engineer human Bub1 and BubR1 proteins integrating kinase and phosphatase activities into one protein and show that these do not support normal mitotic progression. A Bub1–PP2A-B56 complex can support chromosome alignment but results in impairment of the checkpoint due to dephosphorylation of the Mad1 binding site in Bub1. Furthermore, a chimeric BubR1 protein containing the Bub1 kinase domain induces delocalized H2ApT120 phosphorylation, resulting in the reduction of centromeric hSgo2 and chromosome segregation errors. Collectively, these results argue that the spatial separation of kinase and phosphatase activities within the Bub complex is required for balancing its functions in the checkpoint and chromosome alignment.

关键词： mitosis spindle assembly checkpoint kinetochores Bub1 BubR1 PP2A/B56

Sonicating bees demonstrate flexible pollen extraction without instrumental learning

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Current Zoology 2019年第4期65卷 425-436页

作者： Callin M. Switzer Avery l. Russell Daniel R. Papaj Stacey A. Combes robin Hopkins Department of Organismic and Evolutionary Biology Harvard University Cambridge MA USA Arnold Arboretum Harvard University Boston MA USA Department of Ecology and Evolutionary Biology University of Arizona Tucson AZ USA Department of Neurobiology Physiology and Behavior University of California at Davis Davis CA USA Department of Biology and eScience Institute University of Washington Seattle WA USA Department of Biological Sciences University of Pittsburgh Pittsburgh PA USA

Pollen collection is necessary for bee survival and important for flowering plant reproduction, yet if and how pollen extraction motor routines are modified with experience is largely unknown. Here, we used an automated reward and monitoring system to evaluate modification in a common pollen-extraction routine, floral sonication. Through a series of laboratory experiments with the bumblebee, Bombus impatiens, we examined whether variation in sonication frequency and acceleration is due to instrumental learning based on rewards, a fixed behavioral response to rewards, and/or a mechanical constraint. We first investigated whether bees could learn to adjust their sonication frequency in response to pollen rewards given only for specified frequency ranges and found no evidenee of instrumental learning. However, we found that absenee versus receipt of a pollen reward did lead to a predictable behavioral resp on se, which depe nded on bee size. Fin ally, we found some evide nee of mechanical con straints, in that flower mass affected sonication acceleration (but not frequency) through an interaction with bee size. In generalz larger bees showed more flexibility in sonication frequency and acceleration, potentially reflecting a size-based constraint on the range over which smaller bees can modify frequency and accelerati on. Overall our results show that although bees did not display instrumental learning of sonication frequency, their sonication motor routine is nevertheless flexible.

关键词： Bombus impatiens buzz pollination foraging innate behavior learned behavior Solanum

Switchable carbamate coagulants to improve recycling ionic liquid from biomass solutions

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Green Chemical Engineering 2021年第4期2卷 384-391页

作者： Julia l.Shamshina Ying Qin Ken Belmore Daniel T.Daly robin D.Rogers Fiber and Biopolymer Research Institute Department of Plant and Soil ScienceTexas Tech UniversityLubbockTX79409USA Alabama Institute for Manufacturing Excellence The University of AlabamaTuscaloosaAL35487USA Department of Chemistry and Biochemistry The University of AlabamaTuscaloosaAL35487USA College of Arts&Sciences The University of AlabamaTuscaloosaAL35487USA Solutions Inc.P.O.Box 2206TuscaloosaAL35403USA

A reversible amine-carbamate approach has been developed to reduce the use of antisolvents such as water in the coagulation of biopolymers from ionic liquid(Il)solution and thus improve the economy of Il *** and chitin were recovered from 1-ethyl-3-methylimidazolium acetate([C_(2)mim][OAc])solution by introducing the miscible amines triethanolamine(TEA),ethylenediamine(EDA),or butylamine(BA)and bubbling CO_(2) at 40℃ and atmospheric pressure through the solutions to form carbamate salts in situ which resulted in biopolymer *** gave the best results because of its low boiling point and low viscosity,which benefited both biopolymer recovery and Il *** films and fibers could be formed by extrusion of an MCC/[C_(2)mim]-[OAc]solution into a coagulating bath comprised of a 1:1 M mixture of[C_(2)mim][OAc]and butylammonium butylcarbamate(BA-carbamate).The cellulose,Il,amine,and CO_(2) were easily separated,although the cellulose recovered required some water washings to remove traces of *** to 96.4%of the[C_(2)mim][OAc]could be recovered,76.2%from the coagulation bath and 20.2%from the water *** recycled Il was suitable for another cycle of cellulose dissolution and extrusion and 84.6%of the Il used for the second cycle was *** further work is needed,not the least of which will be reducing the amount of water needed for washing steps,some promising features of this process point the way for new directions for more economically viable Il recycling processes in biomass treatment with Ils.

关键词： Carbamate Ionic liquid Recovery Switchable Coagulation Biopolymers Carbamates Il recycle

Whole-blood sorting,enrichment and in situ immunolabeling of cellular subsets using acoustic microstreaming

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Microsystems & Nanoengineering 2018年第1期4卷 457-465页

作者： Neha Garg Trisha M.Westerhof Vick liu robin liu Edward l.Nelson Abraham P.lee Henry Samueli School of Engineering Department of Biomedical EngineeringUniversity of California IrvineIrvineCAUSA School of Medicine Department of MedicineDivision of Hematology/OncologyUniversity of CaliforniaIrvineIrvineCAUSA Massachusetts Institute of Technology CambridgeMAUSA Hochuen Medical Technology Co. LtdArcadiaCAUSA Chao Family Comprehensive Cancer Center Cancer Research InstituteUniversity of CaliforniaIrvineIrvineCAUSA Ayala School of Biological Sciences Department of Molecular Biology&BiochemistryUniversity of CaliforniaIrvineIrvineCAUSA Department of Mechanical and Aerospace Engineering University of CaliforniaIrvineIrvineCAUSA Center for Advanced Design and Manufacturing of Integrated Microfluidics University of CaliforniaIrvineIrvineCAUSA

Analyzing undiluted whole human blood is a challenge due to its complex composition of hematopoietic cellular populations,nucleic acids,metabolites,and *** present a novel multi-functional microfluidic acoustic streaming platform that enables sorting,enrichment and in situ identification of cellular subsets from whole *** single device platform,based on lateral cavity acoustic transducers(lCAT),enables(1)the sorting of undiluted donor whole blood into its cellular subsets(platelets,RBCs,and WBCs),(2)the enrichment and retrieval of breast cancer cells(MCF-7)spiked in donor whole blood at rare cell relevant concentrations(10 ml^(−1)),and(3)on-chip immunofluorescent labeling for the detection of specific target cellular populations by their known marker expression *** approach thus demonstrates a compact system that integrates upstream sample processing with downstream separation/enrichment,to carry out multi-parametric cell analysis for blood-based diagnosis and liquid biopsy blood sampling.

关键词： cell sorting cell enrichment in situ labeling lateral cavity acoustic transducers microstreaming whole blood

Muscle force and movement variability before and after total knee arthroplasty: A review

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World Journal of Orthopedics 2014年第2期5卷 69-79页

作者： Jessica W Smith Jesse C Christensen robin l Marcus Paul C laStayo Department of Bioengineering University of Utah Salt Lake City UT 84108-1290 United States Department of Physical Therapy University of Utah Salt Lake City UT 84108-1290 United States Department of Orthopedics University of Utah Salt Lake City UT 84108-1290 United States Department of Exercise and Sport Science University of Utah Salt Lake City UT 84108-1290 United States

Variability in muscle force output and movement variability are important aspects of identifying individuals with mobility deficits, central nervous system impairments, and future risk of falling. This has been investigated in elderly healthy and impaired adults, as well as in adults with osteoarthritis(OA), but the question of whether the same correlations also apply to those who have undergone a surgical intervention such as total knee arthroplasty(TKA) is still being investigated. While there is a growing body of literature identifying potential rehabilitation targets for individuals who have undergone TKA, it is important to first understand the underlying post-operative impairments to more efficiently target functional deficits that may lead to improvedlong-term outcomes. The purpose of this article is to review the potential role of muscle force output and movement variability in TKA recipients. The narrative review relies on existing literature in elderly healthy and impaired individuals, as well as in those with OA before and following TKA. The variables that may predict longterm functional abilities and deficits are discussed in the context of existing literature in healthy older adults and older adults with OA and following TKA, as well as the role future research in this field may play in providing evidence-based data for improved rehabilitation targets.

关键词： Osteoarthritis Elderly Total knee arthroplasty Movement variability