Whole-blood sorting,enrichment and in situ immunolabeling of cellular subsets using acoustic microstreaming
作者机构:Henry Samueli School of EngineeringDepartment of Biomedical EngineeringUniversity of California IrvineIrvineCAUSA School of MedicineDepartment of MedicineDivision of Hematology/OncologyUniversity of CaliforniaIrvineIrvineCAUSA Massachusetts Institute of TechnologyCambridgeMAUSA Hochuen Medical Technology Co.LtdArcadiaCAUSA Chao Family Comprehensive Cancer CenterCancer Research InstituteUniversity of CaliforniaIrvineIrvineCAUSA Ayala School of Biological SciencesDepartment of Molecular Biology&BiochemistryUniversity of CaliforniaIrvineIrvineCAUSA Department of Mechanical and Aerospace EngineeringUniversity of CaliforniaIrvineIrvineCAUSA Center for Advanced Design and Manufacturing of Integrated MicrofluidicsUniversity of CaliforniaIrvineIrvineCAUSA
出 版 物:《Microsystems & Nanoengineering》 (微系统与纳米工程(英文))
年 卷 期:2018年第4卷第1期
页 面:457-465页
核心收录:
学科分类:1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金:This work was supported by the NSF Center for Advanced Design and Manufacturing of Integrated Microfluidics(CADMIM)(Award Nos.IIP-1362165 and IIP-1362048) Schlumberger Faculty for the Future Award(Award No.SF-202940) the National Cancer Institute of the National Institutes of Health under award no.P30CA062203
主 题:cell sorting cell enrichment in situ labeling lateral cavity acoustic transducers microstreaming whole blood
摘 要:Analyzing undiluted whole human blood is a challenge due to its complex composition of hematopoietic cellular populations,nucleic acids,metabolites,and *** present a novel multi-functional microfluidic acoustic streaming platform that enables sorting,enrichment and in situ identification of cellular subsets from whole *** single device platform,based on lateral cavity acoustic transducers(LCAT),enables(1)the sorting of undiluted donor whole blood into its cellular subsets(platelets,RBCs,and WBCs),(2)the enrichment and retrieval of breast cancer cells(MCF-7)spiked in donor whole blood at rare cell relevant concentrations(10 mL^(−1)),and(3)on-chip immunofluorescent labeling for the detection of specific target cellular populations by their known marker expression *** approach thus demonstrates a compact system that integrates upstream sample processing with downstream separation/enrichment,to carry out multi-parametric cell analysis for blood-based diagnosis and liquid biopsy blood sampling.