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Protein & Cell 2010年第6期1卷 563-572页

作者： Anne jäkel Howard Clark Kenneth B.M.Reid robert B.Sim Department of Pharmacology University of OxfordMansfield RoadOxford OX13QTUK University of Southampton School of MedicineDivision of Infection Inflammation&ImmunitySouthampton General HospitalSouthampton SO166YDUK MRC Immunochemistry Unit Department of BiochemistryUniversity of OxfordSouth Parks RoadOxford OX13QUUK

Surfactant proteins A(SP-A)and D(SP-D),both members of the collectin family,play a well established role in apoptotic cell recognition and *** in vitro data show that SP-A and SP-D interact with apoptotic neutrophils in a distinct ***-A and SP-D bind in a Ca^(2+)-dependent manner to viable and early apoptotic neutrophils whereas the much greater interaction with late apoptotic neutrophils is Ca^(2+)-*** surface molecules on the apoptotic target cells responsible for these interactions had not been identified and this study was done to find candidate target ***(MPO),a specific intracellular defense molecule of neutrophils that becomes exposed on the outside of the cell upon apoptosis,was identified by affinity purification,mass-spectrometry and western blotting as a novel binding molecule for SP-A and *** confirm its role in recognition,it was shown that purified immobilised MPO binds SP-A and SP-D,and that MPO is surface-exposed on late apoptotic ***-A and SP-D inhibit binding of an anti-MPO monoclonal Ab to late apoptotic *** microscopy confirmed that anti-MPO mAb and SP-A/SP-D colocalise on late apoptotic *** was identified as a further potential ligand for SP-A,and neutrophil defensin as a target for both proteins.

关键词： SP-A SP-D myeloperoxidase neutrophils flow cytometry mass spectrometry

On-line non-intrusive particle size measurement of pulverised fuel through digital imaging

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仪器仪表学报 2007年第11期28卷 1942-1946页

作者： Carter robert M Department of Electronics University of Kent

This paper presents the basic principles of particle size measurement and latest industrial results recorded using an innovative optical instrumentation system designed to measure the size distribution of particles in a pneumatic *** system is non-intrusive and cost-effective.A low-cost CCD camera is used to capture images of the particulate flow field,which is illuminated by a low-cost pulsed laser sheet *** particle size distribution is then determined by processing the particle images through the use of novel processing *** results obtained in the past on a small scale particle flow test rig have demonstrated that the system is capable of measuring the size distribution of pneumatically conveyed particles with an accuracy of a few *** the present paper results obtained when testing the system at a 4 MW industrial test facility are *** are made with both off-line reference data achieved through sieving and on-line laser diffraction data recorded using an intrusive,extractive,Malvern Instruments *** general there is good agreement between results when considering the characteristics and limitations of the individual *** novel imaging system shows itself to be rugged,practical and useful under genuine industrial conditions.

关键词：粒子测量方法数字成像数码

Development of a multivariate empirical model for predicting weak rock mass modulus

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International journal of Mining Science and Technology 2015年第4期25卷 545-552页

作者： Kallu Raj R. Keffeler Evan R. Watters robert j. Agharazi Alireza Department of Mining Engineering University of Nevada RESPEC Consulting and Services University of Nevada Itasca Houston Inc.

Estimating weak rock mass modulus has historically proven difficult although this mechanical property is an important input to many types of geotechnical analyses. An empirical database of weak rock mass modulus with associated detailed geotechnical parameters was assembled from plate loading tests per- formed at underground mines in Nevada, the Bakhtiary Dam project, and Portugues Dam project. The database was used to assess the accuracy of published single-variate models and to develop a multivari- ate model for predicting in-situ weak rock mass modulus when limited geoteehnical data are available. Only two of the published models were adequate for predicting modulus of weak rock masses over lim- ited ranges of alteration intensities, and none of the models provided good estimates of modulus over a range of geotechnical properties. In light of this shortcoming, a multivariate model was developed from the weak rock mass modulus dataset, and the new model is exponential in form and has the following independent variables：（1） average block size or joint spacing, （2） field estimated rock strength, （3） dis- continuity roughness, and （4） discontinuity infilling hardness. The multivariate model provided better estimates of modulus for both hard-blocky rock masses and intensely-altered rock masses.

关键词： In-situ modulus Weak rock mass Preliminary design Elastic deformation

Targeted principle component analysis:A new motion artifact correction approach for near-infrared spectroscopy

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journal of Innovative Optical Health Sciences 2014年第2期7卷 98-105页

作者： Meryen A.Yücel julitte Selb robert j.C.ooper David A.Boas Athinoula A.Martinos Center for Biomedical Imaging Department of Radiology Massachusetts General Hospital Haruard Medical SchoolCharlestownMAUSA Department of Medical Physics and Bioengineering University College London LondonUK

As near-infrared spectroscopy(NIRS)broadens its application area to diferent age and diseasegroups,motion artifacts in the NIRS signal due to subject movement is becoming an *** artifacts generally produce signal fiuctuations that are larger than physio-logical NIRS signals,thus it is cruciai to corect for them before obtaining an cstimate ofstimulusevoked hemodynamic responses.,There are various methods for correction such as principlecomponent analy sis(P CA),wavelet-based filt ering and spline int ***,we introduce anew approach to motion artifact correction,targeted principle component analysis(PCA),which incorporates a PCA filter only on the segments of data identified as motion *** expected that this will overcome the issues of filtering desired signals that plagues standardPCA fitering of entire data *** compared the new approach with the most efiective motionartifact correction algorithms on a set of data acquired simultaneously with a collodion-fixedprobe(low motion artifact content)and a standard Velcro probe(high motion artifact content).Our results show that tPCA gives statistically better results in recovering hemodynamic responsefunction(HRF)as compared to wavelet-based fltering and spline interpolation for the *** resulis in a significant reduction in mean-squauared'error(MSE)and significant en-hancement in Pearson's correlation coeficient to the true HRF,The collodion-fixed fiber probewith no motion correction performed better than the Velcro probe corrected for motion artifactsin terms of MSE and Pearson's correlation ***,if the experimental study permits,the use of a collodion-fixed fiber probe may be desirable.I the use of a collodion-fixed probe is notfeasible,then we suggest the use of tP CA in the processing of motion artifact contaminated data.

关键词： Wavelet spline collodion-fixed fiber.

Plant Carbonic Anhydrases： Structures, Locations, Evolution, and Physiological Roles

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Molecular Plant 2017年第1期10卷 30-46页

作者： robert j. DiMario Harmony Clayton Ananya Mukhedee Martha Ludwig james V. Moroney Department of Biological Sciences Louisiana State University Baton Rouge LA 70803 USA School of Chemistry and Biochemistry University of Western Australia Perth WA 6009 Australia

Carbonic anhydrases （CAs） are zinc metalloenzymes that catalyze the interconversion of CO2 and HCO3- and are ubiquitous in nature. Higher plants contain three evolutionarily distinct CA families, αCAs, 13CAs, and γCAs, where each family is represented by multiple isoforms in all species. Alternative splicing of CA transcripts appears common; consequently, the number of functional CA isoforms in a species may exceed the number of genes. CAs are expressed in numerous plant tissues and in different cellular locations. The most prevalent CAs are those in the chloroplast, cytosol, and mitochondria. This diversity in location is paralleled in the many physiological and biochemical roles that CAs play in plants. In this review, the number and types of CAs in C3, C4, and crassulacean acid metabolism （CAM） plants are considered, and the roles of the α and yCAs are briefly discussed. The remainder of the review focuses on plant βCAs and includes the identification of homologs between species using phylogenetic approaches, a consideration of the inter- and intracellular localization of the proteins, along with the evidence for alternative splice forms. Current understanding of βCA tissue-specific expression patterns and what controls them are reviewed, and the physiological roles for which βCAs have been implicated are presented.

关键词： carbonic anhydrase regulation alternative splicing physiological role

Hypocrellin A-based photodynamic action induces apoptosis in A549 cells through ROS-mediated mitochondrial signaling pathway

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Acta Pharmaceutica Sinica B 2019年第2期9卷 279-293页

作者： Shanshan Qi Lingyuan Guo Shuzhen Yan robert j.Lee Shuqin Yu Shuanglin Chen jiangsu Province Key Laboratory for Microbes and Functional Genomics College of Life SciencesNanjing Normal University College of Pharmacy the Ohio State University Cancer Pharmacology Crown Bioscience Inc. jiangsu Province Key Laboratory for Molecular and Medical Biotechnology College of Life SciencesNanjing Normal University

Over recent decades, many studies have reported that hypocrellin A(HA) can eliminate cancer cells with proper irradiation in several cancer cell lines. However, the precise molecular mechanism underlying its anticancer effect has not been fully defined. HA-mediated cytotoxicity and apoptosis in human lung adenocarcinoma A549 cells were evaluated after photodynamic therapy(PDT). A temporal quantitative proteomics approach by isobaric tag for relative and absolute quantitation(iTRAQ) 2 D liquid chromatography with tandem mass spectrometric(LC–MS/MS) was introduced to help clarify molecular cytotoxic mechanisms and identify candidate targets of HA-induced apoptotic cell death. Specific caspaseinhibitors were used to further elucidate the molecular pathway underlying apoptosis in PDT-treated A549 cells. Finally, down-stream apoptosis-related protein was evaluated. Apoptosis induced by HA was associated with cell shrinkage, externalization of cell membrane phosphatidylserine, DNA fragmentation,and mitochondrial disruption, which were preceded by increased intracellular reactive oxygen species(ROS) generations. Further studies showed that PDT treatment with 0.08 mmol/L HA resulted in mitochondrial disruption, pronounced release of cytochrome c, and activation of caspase-3,-9, ***, HA may be a possible therapeutic agent directed toward mitochondria and a promising photodynamic anticancer candidate for further evaluation.

关键词： Hypocrellin A Photodynamic therapy Reactive oxygen species Proteomic LC–MS/MS iTRAQ

Pyramiding disease resistance genes in elite winter wheat germplasm for Western Canada

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The Crop journal 2019年第6期7卷 739-749页

作者： AndréLaroche Michele Frick robert j.Graf jamie Larsen john D.Laurie Agriculture and Agri-Food Canada Lethbridge Research and Development CentreLethbridgeAlberta T1J 4B1Canada Agriculture and Agri-Food Canada Harrow Research and Development Centre2585 County Road 20HarrowOntario N0R 1G0Canada

We report on pyramiding different disease resistance genes against fungal pathogens in Canadian winter wheat germplasm based on available DNA markers and gene *** resistance represents a safe, economical and ecological method for protecting plants, growers and the health of consumers. Major diseases of wheat on the Canadian Prairies are common bunt, rusts(leaf, stem and stripe) and Fusarium head blight. Over the years markers for resistance genes against these diseases have been identified and used by the international wheat community. We describe markers that we have used to pyramid different resistance genes and indicate their presence in Canadian winter wheat cultivars issued from the winter wheat breeding program at the Agriculture and Agri-Food Canada,Lethbridge Research and Development Centre, the only winter wheat breeding program in Western Canada actively delivering new varieties for all regions of the Canadian *** sources of resistance and identities of PCR primers and amplification conditions are indicated to enable the transfer and pyramiding of different resistance(R) genes to breeding lines. We conclude by reviewing new tools for identifying R genes in wheat and indicate how mutagenesis and gene editing can help future efforts to extend the protection offered by known R genes.

关键词： Bunt Gene pyramiding Wheat rusts Marker-assisted selection Triticum aestivum

Parameterized Algorithmics for Computational Social Choice:Nine Research Challenges

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Tsinghua Science and Technology 2014年第4期19卷 358-373页

作者： robert Bredereck jiehua Chen Piotr Faliszewski jiong Guo Rolf Niedermeier Gerhard j.Woeginger the Institut fr Softwaretechnik und Theoretische Informatik TU Berlin Berlin Germany AGH University of Science and Technology Kraków Poland the Cluster of Excellence Multimodal Computing and Interaction Universitt des SaarlandesSaarbrcken Germany the Department of Mathematics and Computer Science TU Eindhoven Eindhoven The Netherlands

Computational Social Choice is an interdisciplinary research area involving Economics, Political Science,and Social Science on the one side, and Mathematics and Computer Science（including Artificial Intelligence and Multiagent Systems） on the other side. Typical computational problems studied in this field include the vulnerability of voting procedures against attacks, or preference aggregation in multi-agent systems. Parameterized Algorithmics is a subfield of Theoretical Computer Science seeking to exploit meaningful problem-specific parameters in order to identify tractable special cases of in general computationally hard problems. In this paper, we propose nine of our favorite research challenges concerning the parameterized complexity of problems appearing in this context. This work is dedicated to jianer Chen, one of the strongest problem solvers in the history of parameterized algorithmics,on the occasion of his 60 th birthday.

关键词： NP-hard problems parameterized complexity fixed-parameter tractability kernelization exact algorithms voting decision making cake cutting

Acute effects of rotavirus and malnutrition on intestinal barrier function in neonatal piglets

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World journal of Gastroenterology 2013年第31期19卷 5094-5102页

作者： Sheila K jacobi Adam j Moeser Anthony T Blikslager j Marc Rhoads Benjamin A Corl robert j Harrell jack Odle Laboratory of Developmental Nutrition College of Agriculture and Life Sciences North Carolina State University Raleigh NC 27695United States College of Veterinary Medicine North Carolina State University Raleigh NC 27695United States Department of Pediatrics University of Texas Health Science Center at Houston Medical School Houston TX 77030 United States Department of Dairy Science Virginia Poly-technic Institute and State University Blacksburg VA 24061United States

AIM: To investigate the effect of protein-energy malnutrition on intestinal barrier function during rotavirus enteritis in a piglet ***: Newborn piglets were allotted at day 4 of age to the following treatments:(1) full-strength formula(FSF)/noninfected;(2) FSF/rotavirus infected;(3) half-strength formula(HSF)/noninfected;or(4) HSF/rotavirus *** one day of adjustment to the feeding rates,pigs were infected with rotavirus and acute effects on growth and diarrhea were monitored for 3 d and jejunal samples were collected for Ussingchamber ***: Piglets that were malnourished or infected had lower body weights on days 2 and 3 post-infection(P junction protein,occludin,in the cell membrane and increased localization in the ***: Overall,malnutrition had no additive effects to rotavirus infection on intestinal barrier function at day 3 post-infection in a neonatal piglet model.

关键词： Rotavirus gastroenteritis Kwashiorkor Occludin Ussing chamber Villus

Advances in alcoholic liver disease:An update on alcoholic hepatitis

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World journal of Gastroenterology 2015年第42期21卷 11893-11903页

作者： Randy Liang Andy Liu Ryan B Perumpail robert j Wong Aijaz Ahmed Department of Medicine Santa Clara Valley Medical Center Albert Einstein College of Medicine Division of Gastroenterology and Hepatology Stanford University School of Medicine Division of Gastroenterology and Hepatology Alameda Health System-Highland Hospital Campus

Alcoholic hepatitis is a pro-inflammatory chronic liver disease that is associated with high short-term morbidity and mortality(25%-35% in one month) in the setting of chronic alcohol use. Histopathology is notable for micro- and macrovesicular steatosis, acute inflammation with neutrophil infiltration, hepatocellular necrosis, perivenular and perisinusoidal fibrosis, and Mallory hyaline bodies found in ballooned hepatocytes. Other findings include the characteristic eosinophilic fibrillar material(Mallory's hyaline bodies) found in ballooned hepatocytes. The presence of focal intense lobular infiltration of neutrophils is what typically distinguishes alcoholic hepatitis from other forms of hepatitis, in which the inflammatory infiltrate is primarily composed of mononuclear cells. Management consists of a multidisciplinary approach including alcohol cessation, fluid and electrolyte correction, treatment of alcohol withdrawal, and pharmacological therapy based on the severity of the disease. Pharmacological treatment for severe alcoholic hepatitis, as defined by Maddrey's discriminant factor ≥ 32, consists of either prednisolone or pentoxifylline for a period of four weeks. The body of evidence for corticosteroids has been greater than pentoxifylline, although there are higher risks of complications. Recently head-to-head trials between corticosteroids and pentoxifylline have been performed, which again suggests that corticosteroids should strongly be considered over pentoxifylline.

关键词： Alcoholic Hepatitis Maddrey discriminant function