Acute effects of rotavirus and malnutrition on intestinal barrier function in neonatal piglets

作     者：Sheila K Jacobi Adam J Moeser Anthony T Blikslager J Marc Rhoads Benjamin A Corl Robert J Harrell Jack Odle 

作者机构：Laboratory of Developmental Nutrition College of Agriculture and Life Sciences North Carolina State University Raleigh NC 27695United States College of Veterinary Medicine North Carolina State University Raleigh NC 27695United States Department of Pediatrics University of Texas Health Science Center at Houston Medical School Houston TX 77030 United States Department of Dairy Science Virginia Poly-technic Institute and State University Blacksburg VA 24061United States 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2013年第19卷第31期

页      面：5094-5102页

核心收录：

学科分类：090603[农学-临床兽医学] 1002[医学-临床医学] 09[农学] 0906[农学-兽医学] 100202[医学-儿科学] 10[医学] 

基　　金：Supported by Cooperative State Research,Education and Extension Service,USDA-National Research Initiative,No.2005-35200-16174 the North Carolina Agriculture Research Service 

主　　题：Rotavirus gastroenteritis Kwashiorkor Occludin Ussing chamber Villus 

摘      要：AIM: To investigate the effect of protein-energy malnutrition on intestinal barrier function during rotavirus enteritis in a piglet ***: Newborn piglets were allotted at day 4 of age to the following treatments:(1) full-strength formula(FSF)/noninfected;(2) FSF/rotavirus infected;(3) half-strength formula(HSF)/noninfected;or(4) HSF/rotavirus *** one day of adjustment to the feeding rates,pigs were infected with rotavirus and acute effects on growth and diarrhea were monitored for 3 d and jejunal samples were collected for Ussingchamber ***: Piglets that were malnourished or infected had lower body weights on days 2 and 3 post-infection(P 0.05).Three days post-infection,marked diarrhea and weight loss were accompanied by sharp reductions in villus height(59%) and lactase activity(91%) and increased crypt depth(21%) in infected compared with non-infected pigs(P 0.05).Malnutrition also increased crypt depth(21%) compared to full-fed ***:crypt ratio was reduced(67%) with viral *** was a trend for reduction in transepithelial electrical resistance with rotavirus infection and malnutrition(P = 0.1).3H-mannitol flux was significantly increased(50%;P 0.001) in rotavirus-infected piglets compared to non-infected piglets,but there was no effect of nutritional ***,rotavirus infection reduced localization of the tight junction protein,occludin,in the cell membrane and increased localization in the ***: Overall,malnutrition had no additive effects to rotavirus infection on intestinal barrier function at day 3 post-infection in a neonatal piglet model.