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Expression and activity of heterologous hydroxyisocaproate dehydrogenases in Synechocystis *** 6803ΔhoxYH

Engineering Microbiology 2022年第1期2卷 1-7页

作者： Valentina Jurka Christoph K.Winkler Silvan Poschenrieder Paulo Oliveira Catarina C.Pacheco Eunice A.Ferreira Florian Weissensteiner Piera De Santis Selin Kara robert Kourist Paula Tamagnini Wolfgang Kroutil a Institute of Chemistry University of GrazNAWI GrazHeinrichstrasse 288010 GrazAustria i3S–Instituto de Investigação e Inovação em Saúde Universidade do Porto4200-135 PortoPortugal IBMC–Instituto de Biologia Molecular e Celular Universidade do Porto4200-135 PortoPortugal Departamento de Biologia Faculdade de CiênciasUniversidade do Porto4169-007 PortoPortugal ICBAS–Instituto de Ciências Biomédicas Abel Salazar Universidade do Porto4050-313 PortoPortugal Aarhus University Department of EngineeringBiological and Chemical Engineering SectionBiocatalysis and Bioprocessing GroupGustav Wieds Vej 10DK 8000 AarhusDenmark Institute of Molecular Biotechnology Graz University of Technology8010 GrazAustria Field of Excellence BioHealth University of Graz8010 GrazAustria BioTechMed Graz 8010 GrazAustria.

Exploiting light to drive redox reactions is currently a hot topic since light is considered as an environmentally friendly source of ***,cyanobacteria,which can use light e.g.,for generating NADPH,are in the focus of ***,it has been shown that various heterologous redox enzymes could be expressed in these *** we demonstrated the successful inducer-free expression of𝛼-keto-acid dehydroge-nases(L-HicDH and D-HicDH)from Lactobacillus confusus DSM 20196 and Lactobacillus paracasei DSM 20008 in Synechocystis *** 6803ΔhoxYH mutant using replicative *** the L-HicDH showed poor activity limited by the amount of expressed enzyme,the D-HicDH was applied both in vivo and in vitro,transforming the selected𝛼-keto acids to the corresponding optically pure(R)-𝛼-hydroxy acids(ee>99%)in up to 53%and 90%conversion,respectively.

关键词： Cyanobacteria Biocatalysis Photobiotransformation Synechocystis sp.PCC 6803 Hydroxyisocaproate dehydrogenase Ketoacid dehydrogenase

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空间站的实时仿真

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系统仿真学报 1990年第1期2卷 24-33页

作者： robert H.John Gerard J.Moorman Blaine W.Brown

实时、载人回路的仿真在NASA载人空间飞行计划中起着非常重要的作用,它可以在地面通过模拟的飞行环境对系统设计和整个系统性能作出评价.在航天飞机的各个开发阶段,直至投入运行很大程度要依靠仿真技术.经过16年计划的实施,在NASA Ly nd... 详细信息

实时、载人回路的仿真在NASA载人空间飞行计划中起着非常重要的作用,它可以在地面通过模拟的飞行环境对系统设计和整个系统性能作出评价.在航天飞机的各个开发阶段,直至投入运行很大程度要依靠仿真技术.经过16年计划的实施,在NASA Ly ndon B Johnson空间中心(JSC)工程部门中,用于支持航天飞机开发和运行的仿真设施已得到改进,它反映了计算机系统和仿真软件设计方面的最新技术.在1985年,JSC工程仿真设施系统工程仿真器得到了扩充,以支持与轨道飞行器有关的空间站设计活动,例如:轨道飞行器的对接方案,利用轨道飞行器远程操纵系统停靠空间站的方案.为了长期支持空间站计划,新的空间站的仿真开发工作目前正在顺利进行.本文介绍了工程部门中的两个仿真设施,即系统工程仿真器和航天飞机电子系统总体实验室;讨论了它们在航天飞机计划中的作用,特别是能够应用到空间站方面的那些功能;同时阐述了系统工程仿真器在空间站开发计划中的作用.最后,详尽地叙述了系统工程仿真器上正在开发的新的空间站仿真.

关键词：空间站实时仿真航天站

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Perspectives on perioperative management of children’s surgical conditions during the COVID-19 pandemic in low-income and middle-income countries:a global survey

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World Journal of Pediatric Surgery 2020年第3期3卷 29-36页

作者： Paul Truche Alexis Bowder Amber Trujillo Lalla robert Crum Fabio Botelho Henry Elliot Rice Bellisa Caldas Lopes Sarah Greenberg Faye Evans John Gerard Meara Emmanuel Adoyi Ameh David Patrick Mooney Program in Global Surgery and Social Change Harvard Medical SchoolBostonMassachusettsUSA Department of Surgery Boston Children's HospitalBostonMassachusettsUSA University of New Mexico School of Medicine AlbuquerqueNew MexicoUSA Hospital das Clinicas da Universidade Federal de Minas Gerais Belo HorizonteMinas GeraisBrazil Division of Pediatric General Surgery Duke Children's Hospital and Health CenterDurhamNorth CarolinaUSA Department of Pediatric Surgery Instituto de Medicina Integral Prof.Fernando FigueiraRecifeBrazil Department of Surgery University of WashingtonSeattleWashingtonUSA Division of Pediatric General&Thoracic Surgery Seattle Children’s HospitalSeattleWashingtonUSA Department of Anesthesiology Critical Care and Pain MedicineBoston Children's HospitalBostonMassachusettsUSA Department of Plastic and Oral Surgery Boston Children's HospitalBostonMassachusettsUSA Division of Paediatric Surgery Department of SurgeryNational HospitalAbujaNigeria

Background Many organizations have issued recommendations to limit elective surgery during the coronavirus disease 2019(COVID-19)*** surveyed providers of children’s surgical care working in low-income and middle-income countries(LMICs)to understand their perspectives on surgical management in the wake of the COVID-19 pandemic and how they were subsequently modifying their surgical care *** A survey of children’s surgery providers in LMICs was *** reported how their perioperative practice had changed in response to *** were also presented with 26 specific procedures and asked which of these procedures they were allowed to perform and which they felt they should be allowed to *** in surgical practice reported by respondents were analyzed *** A total of 132 responses were obtained from 120 unique institutions across 30 LMICs.117/120 institutions(97.5%)had issued formal guidance on delaying or limiting elective children’s surgical *** in LICs were less likely to have issued guidance on elective surgery compared with middle-income facilities(82%in LICs vs 99%in lower middle-income countries and 100%in upper middle-income countries,p=0.036).Although 122(97%)providers believed cases should be limited during a global pandemic,there was no procedure where more than 61%of providers agreed cases should be delayed or *** There is little consensus on which procedures should be limited or delayed among LMIC *** of testing capacity and local,context-specific guidelines may be a better strategy than international consensus,given the disparities in availability of preoperative testing and the lack of consensus towards which procedures should be delayed.

关键词： income survey testing

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中国木业吸引了第一流的国外公司——眼中的“2003中国木制品进出口贸易洽谈会”

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国外林产工业文摘 2003年第12期33卷 1-4页

作者： By robert Flynn, Wood Resources International 美国木材资源国际公司(WRI)

我非常高兴参加了今年的"CWEIC",所有到会者一致认为这是一个成功的会议.因为我介绍了很多国外公司(他们在会上简要介绍了公司的情况),我对于国外到会公司的高质量印象深刻.总的来说,这些公司在成为产品的潜在客户方面,在原材料来源方面... 详细信息

我非常高兴参加了今年的"CWEIC",所有到会者一致认为这是一个成功的会议.因为我介绍了很多国外公司(他们在会上简要介绍了公司的情况),我对于国外到会公司的高质量印象深刻.总的来说,这些公司在成为产品的潜在客户方面,在原材料来源方面,在成为合资伙伴方面,对于中国的企业意味着巨大潜力.对于那些没能来参加今年会议的公司,我想介绍一些前来参会的非常重要的公司,从而使你们明了这次会议(包括明年的会议)对于中国木制品工业的潜在影响力.

关键词： Georgia-Pacific公司 Woodgrain Millwork公司惠好-西北硬木公司 “2003中国木制品进出口贸易洽谈会”

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猪的非常规饲料原料(一)

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中国畜牧杂志 2009年第6期45卷 36-40页

作者：武书庚 robert O.Myer Joel H.Brendemhl 中国农业科学院饲料研究所北京100081

猪饲料一般由谷物和油籽粕组成，但猪能利用多种饲料原料，包括食品加工、配菜和服务业的副（共）产品或可食废弃物，如谷物加工、酿造和蒸馏、烘焙、水果和蔬菜加工、肉蛋奶加工、海产品加工、精细食品制造业、食品零售等。猪饲料不常... 详细信息

猪饲料一般由谷物和油籽粕组成，但猪能利用多种饲料原料，包括食品加工、配菜和服务业的副（共）产品或可食废弃物，如谷物加工、酿造和蒸馏、烘焙、水果和蔬菜加工、肉蛋奶加工、海产品加工、精细食品制造业、食品零售等。猪饲料不常用的原料还包括在低价格、过剩或传统原料不足时使用的原料，及传统饲料不足而能经济替代的当地丰富的原料。

关键词：非常规饲料原料猪饲料海产品加工食品加工谷物加工食品制造业蔬菜加工废弃物

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International Medical Geology Association （IMGA） formed

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Episodes 2006年第1期29卷 67-67页

作者： Dr. Olle Selinus Dr. Jose Centeno Dr. robert Finkelman IMGA Chair IMGA Co-chair for Medical Science IMGA Co-Chair for Geoscience

＂Medical Geology＂ is defined as the science dealing with the relationship between natural geological factors and health problems in man and animals and understanding the influence of ordinary environmental factors on the geographical distribution of such health problems. Medical Geology is therefore a broad and complicated subject and if the problems to be addressed are to be understood, mitigated, or resolved, it wil l require interdisciplinary contributions from different scientific fields （involving geo-scientists, medical scientists, health professionals, veterinarians, and biologists, etc.）. Medical Geology involves the whole geo-sphere and can be considered as dealing with ecosystem health.

关键词：医学地质学地理环境学科建设生态系统

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数字记忆广度对策略应用模式的影响──中美儿童计算能力差异的比较研究

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心理科学 1994年第1期17卷 21-27,63-64页

作者：刘凡中央教育科学研究所

本研究旨在了解中美儿童在策略运用模式上的差异是否与数字记忆广度有关。为此，分别测验了中美幼儿园儿童的加法技能，数字记忆广度，并通过一组计算机呈现的加法作业评估儿童使用的解题策略。结果表明，中国儿童的基本算术技能和数学... 详细信息

本研究旨在了解中美儿童在策略运用模式上的差异是否与数字记忆广度有关。为此，分别测验了中美幼儿园儿童的加法技能，数字记忆广度，并通过一组计算机呈现的加法作业评估儿童使用的解题策略。结果表明，中国儿童的基本算术技能和数学记忆广度均超过同龄的美国儿童。在解题时，中国儿童多使用效率较高的言语计算策略，美国儿童则多采用计数手指策略，在言语计数策略和检索策略的加工速度上中国儿童也超过美国儿童。相关分析表明．策略应用模式的文化差异与数字记忆广度有关。比较大的工作记忆容量增加了数字线索可利用的资源，为儿童早期言语计数能力的发展提供了有利条件。

关键词：数字记忆广度策略应用模式计算能力

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非体外循环下冠状动脉搭桥的临床探讨

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西安医科大学学报 2001年第3期22卷 272-273页

作者：耿希刚 Jorge Carcia robert Gilbert 许锁春郑建杰董安平西安交通大学第一医院心脏外科西安710061 HospitalCenter Washington DCU.S.A

目的　总结非体外循环下冠状动脉搭桥的临床经验。方法　在非体外循环下行冠状动脉搭桥术 1 0例 ,2例左前外侧小切口 ,1例胸骨下段正中小切口 ,7例胸骨正中切口。搭桥支数 1～ 4支 ,平均 ( 2 3± 1 3)支 ,均应用左侧乳内动脉搭于左前... 详细信息

目的　总结非体外循环下冠状动脉搭桥的临床经验。方法　在非体外循环下行冠状动脉搭桥术 1 0例 ,2例左前外侧小切口 ,1例胸骨下段正中小切口 ,7例胸骨正中切口。搭桥支数 1～ 4支 ,平均 ( 2 3± 1 3)支 ,均应用左侧乳内动脉搭于左前降支。结果　 1 0例患者术后均恢复良好 ,痊愈出院 ;平均住院费用下降。 2例出现并发症 ,经治疗痊愈。结论　非体外循环冠状动脉搭桥术创伤小、安全 ,但对手术操作技术要求高 ,需要特殊设备 ,且应具有一定的手术适应症。

关键词：冠状动脉搭桥技术非体外循环胸骨正中切口胸左前外侧小切口胸骨下段正中小切口

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Evaluation of the adjuvanticity of artemisinin with soluble Leishmania major antigens in BALB/c mice

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Journal of Nanjing Medical University 2009年第6期23卷 359-372页

作者： Albert Kimutai Willy K. Tonui Michael M. Gicheru Peter Kamau Ngure Johnstone Ingonga Stella Kepha Laban Ireri Njeru Dorcas Wachira robert Karanja Muhia Milkah Mwangi Lydia B. Nyamwamu Department of Zoological Sciences Kenyatta University Centre for Biotechnology Research and Development Kenya Medical Research Institute Science Department Daystar University School of Biological Sciences University of Nairobi

Objective： To determine the adjuvant potential of artemisinin with a soluble leishmanial antigen in vaccinating BALB/c mice. Methods： Seventy two female BALB/c mice were randomly assigned into six groups. The mice were vaccinated with soluble Leishmania antigens （SLA） alone, artemisinin co-administered with SLA, SLA and Bacille Calmette Gu fin （BCG） vaccine, and artemisinin and BCG alone. Unvaccinated mice formed the control group. The induction of cell-mediated immunity following vaccination was determined by measuring in vitro lymphocyte proliferation and the production of interleukin （IL）-4, IL-5 and gamma interferon （IFN-γ） determined by flow cytometry. Protection against L. major was determined by quantifying parasite burdens in L. major infected footpads using a limiting dilution assay and by measuring lesion sizes of the infected footpad compared to the contralateral uninfected footpad. Results： Mice receiving SLA plus artemisinin produced significantly high levels of IL-4 and IL-5 （P 〈 0.05） and low levels of IFN-γ, resulting in exacerbated disease. In addition, subcutaneous administration of SLA ＋ artemisinin, artemisinin alone or SLA alone resulted in the development of large footpad swellings and high parasite loads that were comparable to those of the control unvaccinated mice （P 〉 0.05）, resulting in exacerbated disease. Conclusion： These data suggest that artemisinin is not a suitable adjuvant for Leishmania vaccines. However, since artemisinin has been shown to be effective against Leishmania parasites in vitro and in vivo, further studies ought to be conducted to determine its immunochemotherapeutic potential when co-administered with Leishmania antigens.

关键词： Artemisinin Leishmania Soluble Leishmania antigens BALB/c mice immunity cytokine adjuvant interleukin-4 (IL-4) IL-5 IFN-γ lesion size footpad lesion vaccine parasite burden proliferation stimulation index

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Identification of Conserved Regulatory Elements in Mammalian Promoter Regions:A Case Study Using the PCK1 Promoter

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Genomics, Proteomics & Bioinformatics 2008年第3期6卷 129-143页

作者： George E. Liui Matthew T. Weirauch Curtis P. Van Tassell robert W. Li Tad S. Sonstegard LakshmiK. Matukumal Erin E. Connor Richard W. Hanson Jianqi Yang Bovine Functional Genomics Laboratory Beltsville Agricultural Research Center Beltsville MD 20705 USA Department of Biomolecular Engineering University of California Santa Cruz Santa Cruz CA 95064 USA Department of Bioinformatics and Computational Biology George Mason University Manassas VA 20110 USA Department of Biochemistry Case Western Reserve University School of Medicine Cleveland OH 44106 USA.

A systematic phylogenetic footprinting approach was performed to identify conserved transcription factor binding sites （TFBSs） in mammalian promoter regions using human, mouse and rat sequence alignments. We found that the score distributions of most binding site models did not follow the Gaussian distribution required by many statistical methods. Therefore, we performed an empirical test to establish the optimal threshold for each model. We gauged our computational predictions by comparing with previously known TFBSs in the PCK1 gene promoter of the cytosolic isoform of phosphoenolpyruvate carboxykinase, and achieved a sensitivity of 75% and a specificity of approximately 32% Almost all known sites overlapped with predicted sites, and several new putative TFBSs were also identified. We validated a predicted SP1 binding site in the control of PCK1 transcription using gel shift and reporter assays. Finally, we applied our computational approach to the prediction of putative TFBSs within the promoter regions of all available RefSeq genes. Our full set of TFBS predictions is freely available at http：//***/tfbsConsSites.

关键词： phylogenetic footprinting phosphoenolpyruvate carboxykinase transcription factor binding sites mammalian gene promoters

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