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Atomic force microscopy correlates antimetastatic potentials of HepG2 cell line with its redox/energy status: effects of curcumin and Khaya senegalensis

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Journal of Integrative Medicine 2017年第3期15卷 214-230页

作者： Jeremiah Olorunjuwon Olugbami robert damoiseaux Bryan France Michael A. Gbadegesin Adam Z. Stieg Shivani Sharma Oyeronke A. Odunola James K. Gimzewski Cancer Research and Molecular Biology Laboratories Department of Biochemistry College of Medicine University of Ibadan Ibadan Oyo 200005 Nigeria Nano and Pico Characterization Laboratories California NanoSystems Institute University of California LosAngeles California 90095 USA Department of Chemistry and Biochemistry University of California Los Angeles California 90095 USA Molecular Screening and Shared Resources Laboratories California NanoSystems Institute University of California Los Angeles California 90095 USA Department of Molecular and Medicinal Pharmacology University of California Los Angeles California 90095 USA International Center for Materials Nanoarchitectonics Satellite National Institute for Materials Science Tsukuba 3690114 Japan

OBJECTIVE： The fatality of cancer is mostly dependent on the possibility of occurrence of metastasis. Thus, if the development of metastasis can be prevented through novel therapeutic strategies targeted against this process, then the success of cancer treatment will drastically increase. In this study, therefore, we evaluated the antimetastatic potentials of an extract of Khaya senegalensis and curcumin on the metastatic liver cell line HepG2, and also assessed the anticancer property of the extract. METHODS： Cells were cultured and treated with graded concentrations of test substances for 24, 48, or 72 h with provisions made for negative controls. Treated cells were assessed as follows： nanotechnologically--atomic force microscopy （AFM） was used to determine cell stiffness; biochemically--cell cytotoxicity, glutathione level and adenosine triphosphate status, caspase activation and mitochondrial toxicity were considered; and microbiologically--a carrot disk assay was used to assess the anticancer property of the extract of K. senegalensis. RESULTS： Curcumin and K. senegalensis increased the cell stiffness by 2.6- and 4.0-fold respectively, indicating their antimetastatic effects. Corresponding changes in redox （glutathione level） and energy （adenosine triphosphate） status of the cells were also demonstrated. Further mechanistic studies indicated that curcumin was not mitotoxic in HepG2 cells unlike the K. senegalensis extract. In addition the extract potently inhibited the Agrobacterium tumefaciens-induced genetic transformation based on carrot disk assay. CONCLUSION： Cell elasticity measurement data, using AFM, strongly suggested, for the first time, that both curcumin and the extract of K. senegalensis exhibited antimetastatic properties on HepG2 cells.

关键词： Curcumin Khaya senegalensis atomic force microscopy glutathione adenosine triphosphate metasis carcinoma, hepatocellular

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Interferon-mediated reprogramming of membrane cholesterol to evade bacterial toxins

Interferon-mediated reprogramming of membrane cholesterol to...

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第四届中国生物物理学会代谢生物学分会学术研讨会

作者： Quan D.Zhou Xun Chi Min Sub Lee Wei Yuan Hsieh Jonathan J.Mkrtchyan An-Chieh Feng Cuiwen He Autumn G York Viet L.Bui Eliza B.Kronenberger Alessandra Ferrari Xu Xiao Allison E.Daly Elizabeth J.Tarling robert damoiseaux Philip O.Scumpia Stephen T.Smale Kevin J.Williams Peter Tontonoz Steven J.Bensinger Zhejiang University School of Medicine

＜正＞Cholesterol is an important determinant of the biochemical and biophysical properties of animal cell membranes. Given the requirement for cholesterol in membranes, it is not surprising that microorganisms and viruses have evolved strategies that take advantage of cholesterol availability in membranes to facilitate pathogenesis. Cholesterol-dependent cytolysins （CDCs） are a group of secreted toxins produced mainly by Gram-positive bacteria that depend on membrane cholesterol for their function.

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