Atomic force microscopy correlates antimetastatic potentials of HepG2 cell line with its redox/energy status: effects of curcumin and Khaya senegalensis

作     者：Jeremiah Olorunjuwon Olugbami Robert Damoiseaux Bryan France Michael A. Gbadegesin Adam Z. Stieg Shivani Sharma Oyeronke A. Odunola James K. Gimzewski 

作者机构：Cancer Research and Molecular Biology Laboratories Department of Biochemistry College of Medicine University of Ibadan Ibadan Oyo 200005 Nigeria Nano and Pico Characterization Laboratories California NanoSystems Institute University of California LosAngeles California 90095 USA Department of Chemistry and Biochemistry University of California Los Angeles California 90095 USA Molecular Screening and Shared Resources Laboratories California NanoSystems Institute University of California Los Angeles California 90095 USA Department of Molecular and Medicinal Pharmacology University of California Los Angeles California 90095 USA International Center for Materials Nanoarchitectonics Satellite National Institute for Materials Science Tsukuba 3690114 Japan 

出 版 物：《Journal of Integrative Medicine》 (结合医学学报（英文版）)

年 卷 期：2017年第15卷第3期

页      面：214-230页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：National Cancer Institute  NCI  (P30CA016042) 

主　　题：Curcumin Khaya senegalensis atomic force microscopy glutathione adenosine triphosphate metasis carcinoma, hepatocellular 

摘      要：OBJECTIVE： The fatality of cancer is mostly dependent on the possibility of occurrence of metastasis. Thus, if the development of metastasis can be prevented through novel therapeutic strategies targeted against this process, then the success of cancer treatment will drastically increase. In this study, therefore, we evaluated the antimetastatic potentials of an extract of Khaya senegalensis and curcumin on the metastatic liver cell line HepG2, and also assessed the anticancer property of the extract. METHODS： Cells were cultured and treated with graded concentrations of test substances for 24, 48, or 72 h with provisions made for negative controls. Treated cells were assessed as follows： nanotechnologically--atomic force microscopy （AFM） was used to determine cell stiffness; biochemically--cell cytotoxicity, glutathione level and adenosine triphosphate status, caspase activation and mitochondrial toxicity were considered; and microbiologically--a carrot disk assay was used to assess the anticancer property of the extract of K. senegalensis. RESULTS： Curcumin and K. senegalensis increased the cell stiffness by 2.6- and 4.0-fold respectively, indicating their antimetastatic effects. Corresponding changes in redox （glutathione level） and energy （adenosine triphosphate） status of the cells were also demonstrated. Further mechanistic studies indicated that curcumin was not mitotoxic in HepG2 cells unlike the K. senegalensis extract. In addition the extract potently inhibited the Agrobacterium tumefaciens-induced genetic transformation based on carrot disk assay. CONCLUSION： Cell elasticity measurement data, using AFM, strongly suggested, for the first time, that both curcumin and the extract of K. senegalensis exhibited antimetastatic properties on HepG2 cells.