检索结果-南通市图书馆

Acta Pharmaceutica Sinica B 2024年第1期14卷 421-432页

作者： Dexiu Yan Jemma Arakelyan Teng Wan Ritvik Raina Tsz Ki Chan Dohyun Ahn Vladimir Kushnarev Tsz Kiu Cheung Ho Ching Chan Inseo Choi Pui Yi Ho Feijun Hu Yujeong kim Hill Lam Lau Ying Lo Law Chi Seng Leung Chun Yin Tong Kai Kap Wong Wing Lam Yim Nikolay S.Karnaukhov richard Y.C.Kong Maria V.Babak Yudai Matsuda Department of Chemistry City University of Hong KongHong Kong SARChina iGEM Team“VarieCure” City University of Hong KongHong Kong SARChina Department of Biomedical Engineering City University of Hong KongHong Kong SARChina Moscow Clinical Research Center Named After A.S.Loginov Moscow 111123Russian Federation

A biosynthetic gene cluster for the bioactive fungal sesterterpenoids variecolin(1)and variecolactone(2)was identified in Aspergillus aculeatus ATCC *** production of 1 and 2 was achieved in Aspergillus oryzae by expressing the sesterterpene synthase VrcA and the cytochrome P450 ***,the replacement of VrcB with homologous P450s from other fungal terpenoid pathways yielded three new variecolin analogues(5-7).Analysis of the compounds’anticancer activity in vitro and in vivo revealed that although 5 and 1 had comparable activities,5 was associated with significantly reduced toxic side effects in cancer-bearing mice,indicating its potentially broader therapeutic *** study describes the first tests of variecolin and its analogues in animals and demonstrates the utility of synthetic biology for creating molecules with improved biological activities.

关键词： Animal studies Anticancer properties Biosynthesis Natural products Sesterterpenoids Synthetic biology Terpene synthases Variecolin

Higher radiation dose with a shorter treatment duration improves outcome for locally advanced carcinoma of anal canal

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2007年第6期13卷 895-900页

作者： kim Huang Daphne Haas-Kogan Vivian Weinberg richard Krieg Department of Radiation Oncology University of California San Francisco CA United States Comprehensive Cancer Center Biostatistics Core University of California San Francisco CA United States

AIM： To assess whether radiation dose and duration of treatment influence local control and survival of patients with locally advanced anal cancer treated with definitive chemoradiation. METHODS： Twenty-eight consecutive patients who were treated with definitive radiation therapy for bulky anal cancers （〉 5 cm in size） were reviewed. Nineteen patients had T3 lesions, 8 patients had T4 lesions, and 15 patients had lymph node involvement. The median tumor size was 7.5 cm. All but one patient received concurrent chemoradiation. The median radiation dose was 54 Gy. The median duration of treatment was 58 d. RESULTS： With a median follow-up of 2.5 years in all patients and 7.8 years in living patients, the 2-year local recurrence-free probability was 570 and overall survival rate was 67%. Neither radiation dose nor duration of treatment alone was predictive of either time to local failure or overall survival. However, longer treatment breaks can potentially mask an advantage over higher radiation doses. Therefore, we examined those patients who received ≥ 54 Gy within 60 d, comparing them to the rest of the patients. Of patients who received ≥54 Gy within 60 d, local progression-free probability was 890 versus 420 for the rest of the group （P = 0.01）. CONCLUSION： Local failure is a significant problem in locally advanced carcinomas of-the anal canal. Higher radiation doses with limited treatment breaks may offer an increase in local control and survival.

关键词： Anal carcinoma Locally advanced Chemoradiation

A Simple Method of Electrospun Tungsten Trioxide Nanofibers with Enhanced Visible-Light Photocatalytic Activity

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano-Micro Letters 2015年第3期7卷 291-297页

作者： Frank Agyemang Ofori Faheem A.Sheikh richard Appiah-Ntiamoah Xinsheng Yang Hern kim Department of Energy Science and Technology Energy and Environment Fusion Technology Center Myongji University Key Laboratory of Advanced Technology of Materials Superconductor and New Energy R&D Center Southwest Jiaotong University

The present study involves the fabrication of tungsten trioxide(WO3) nanofibers by an electrospinning technique using polyvinyl pyrrolidone(PVP)/citric acid/tungstic acid as precursor solution. It was found that the PVP concentration was one of the most crucial processing parameters determining the final properties of WO3 nanofibers. The optimum concentration of PVP was from 75 to 94 g L-1. The average diameter of the nanofibers increases with increasing the PVP concentration, whereas it is decreased after sintering and orthorhombic structure were formed at 500 °C. The photocatalytic properties of the as-synthesized nanofibers were also investigated by degrading methylene blue and twofold efficiency was obtained compared with that of commercial WO3 microparticles.

关键词： Tungsten trioxide Electrospinning Nanofiber Photocatalysis

Observational Diagnosis of Cloud Phase in the Winter Antarctic Atmosphere for Parameterizations in Climate Models

在线全文

学校读者我要写书评

暂无评论

Advances in Atmospheric Sciences 2010年第6期27卷 1233-1245页

作者： Yong-Sang CHOI Chang-Hoi HO Sang-Woo kim richard S.LINDZEN Department of Environmental Science and Engineering Ewha Womans University Department of Earth Atmospheric and Planetary SciencesMassachusetts Institute of Technology School of Earth and Environmental Sciences Seoul National University

The cloud phase composition of cold clouds in the Antarctic atmosphere is explored using data from the Moderate Resolution Imaging Spectroradiometer （MODIS） and Cloud-Aerosol Lidar with Orthogonal Polarization （CALIOP） instruments for the period 2000-2006. We used the averaged fraction of liquid-phase clouds out of the total cloud amount at the cloud tops since the value is comparable in the two measurements. MODIS data for the winter months （June, July, and August） reveal liquid cloud fraction out of the total cloud amount significantly decreases with decreasing cloud-top temperature below 0°C. In addition, the CALIOP vertical profiles show that below the ice clouds, low-lying liquid clouds are distributed over ～20% of the area. With increasing latitude, the liquid cloud fraction decreases as a function of the local temperature. The MODIS-observed relation between the cloud-top liquid fraction and cloud-top temperature is then applied to evaluate the cloud phase parameterization in climate models, in which condensed cloud water is repartitioned between liquid water and ice on the basis of the grid point temperature. It is found that models assuming overly high cut-offs （ -40°C） for the separation of ice clouds from mixed-phase clouds may significantly underestimate the liquid cloud fraction in the winter Antarctic atmosphere. Correction of the bias in the liquid cloud fraction would serve to reduce the large uncertainty in cloud radiative effects.

关键词： cloud phase mixed-phase clouds polar cloud cloud radiative effect cloud parameterization

Contaminated open fracture and crush injury: a murine model

在线全文

学校读者我要写书评

暂无评论

Bone Research 2015年第1期3卷 31-39页

作者： Shawn R Gilbert Justin Camara richard Camara Lynn Duffy Ken Waites Hyunki kim Kurt Zinn Department of Surgery University of Alabama at Birmingham AL USA Loma Linda School of Medicine Loma LindaCAUSA Departments of Pathology University of Alabama at BirminghamAL USA Department of Radiology University of Alabama at BirminghamAL USA

Modern warfare has caused a large number of severe extremity injuries, many of which become infected. In more recent conflicts, a pattern of co-infection with Acinetobacter baumannii and methicillin-resistant Staphylococcus aureus has emerged. We attempted to recreate this pattern in an animal model to evaluate the role of vascularity in contaminated open fractures. Historically, it has been observed that infected bones frequently appear hypovascular, but vascularity in association with bone infection has not been examined in animal models. Adult rats underwent femur fracture and muscle crush injury followed by stabilization and bacterial contamination with A. baumannii complex and methicillin-resistant Staphylococcus *** and perfusion were assessed by micro CT angiography and SPECT scanning, respectively, at 1, 2 and 4 weeks after injury. Quantitative bacterial cultures were also obtained. Multi-bacterial infections were successfully created, with methicillin-resistant S. aureus predominating. There was overall increase in blood flow to injured limbs that was markedly greater in bacteria-inoculated limbs. Vessel volume was greater in the infected group. Quadriceps atrophy was seen in both groups, but was greater in the infected group. In this animal model, infected open fractures had greater perfusion and vascularity than non-infected limbs.

关键词： MRSA Contaminated open fracture and crush injury a murine model

Probiotic effects on intestinal fermentation patterns in patients with irritable bowel syndrome

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2008年第32期14卷 5020-5024页

作者： Jacqueline S Barrett kim EK Canale richard B Gearry Peter M Irving Peter R Gibson Monash University Department of Medicine and Department of Gastroenterology Box Hill Hospital

AIM： To determine whether Lactobacillus casei strain Shirota （Yakult） can alter small intestinal bacterial overgrowth （SIBO）, as tested by the lactulose breath test, and whether this is associated with changes in symptoms in irritable bowel syndrome （IBS）. METHODS： 18 patients with IBS （Rome Ⅱ criteria）, who showed an early rise in breath hydrogen with lactulose （ERBHAL）, consumed 65 mL of Yakult daily for 6 wk. Lactulose breath test was repeated at the end of the treatment period. Symptoms were recorded daily using a 10 cm visual analogue scale. RESULTS： 14 patients completed the study, 9 （64%） had reversal of ERBHAL, with the median time of first rise in breath hydrogen increasing from 45 to 75 min （P = 0.03）. There was no significant improvement in the symptom score with probiotic therapy, except for wind （P = 0.04）. Patients commencing with at least moderate symptoms and who no longer had ERBHAL at the end of treatment, showed improvement in the overall symptoms scores [median final score 5.3 （IQR 3.9-5.9）, 55% reduction; n = 6] to a greater extent than those who had had persisting ERBHAL [final score 6.9 （5.0-7.0）, 12% reduction; n = 5; P = 0.18]. CONCLUSION： Yakult is effective in altering fermentation patterns in the small bowel, consistent with reducing SIBO. The loss of ERBHAL was associated with reduced symptoms. The true interpretation of these findings awaits a randornised, controlled trial.

关键词： Probiotics Small intestinal bacterial overgrowth Breath hydrogen testing Functional gut symptoms

Time-Lapse 3-D Seismic Wave Simulation via the Generalized Multiscale Finite Element Method

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Communications in Computational Physics 2020年第6期28卷 401-423页

作者： Yongchae Cho richard L.Gibson,Jr. Hyunmin kim Mikhail Artemyev Yalchin Efendiev Shell International Exploration&Production Inc. 150 N Dairy Ashford RoadHoustonTX 77079USA Department of Geology&Geophysics Texas A&M UniversityCollege StationTexas 77843-3115USA Shell Exploration&Production Company 701 Poydras StreetNew OrleansLA 70139USA Arista Networks DublinIreland Department of Mathematics Texas A&M UniversityCollege StationTexas 77843-3368USA Multiscale Model Reduction Laboratory North-Eastern Federal UniversityYakutskRepublic of Sakha 677980Russia

Numerical solution of time-lapse seismic monitoring problems can be challenging due to the presence of finely layered *** wave modeling using fine layered meshes also adds more computational *** approaches such as finite difference and finite element methods may be prohibitively expensive if the whole domain is discretized with the cells corresponding to the grid in the reservoir subdomain.A common approach in this case is to use homogenization techniques to upscale properties of subsurface media and assign the background properties to coarser grid;however,inappropriate application of upscaling might result in a distortion of the model,which hinders accurate monitoring of the fluid change in *** this work,we instead investigate capabilities of a multiscale method that can deal with fine scale heterogeneities of the reservoir layer and more coarsely meshed rock properties in the surrounding domains in the same *** address the 3-D wave problems,we also demonstrate how the multiscale wave modeling technique can detect the changes caused by fluid movement while the hydrocarbon production activity proceeds.

关键词： Fluid simulation time lapse Generalized multiscale finite element elastic wave

Laboratory-scale model of carbon dioxide deposition for soilstabilisation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Rock Mechanics and Geotechnical Engineering 2016年第2期8卷 178-186页

作者： Mohammad Hamed Fasihnikoutalab Afshin Asadi Bujang kim Huat PaulWestgate richard JBall Shahram Pourakbar Department of Civil Engineering University Putra Malaysia43400 UPMSerdangSelangorMalaysia Housing Research Centre Department of Civil EngineeringUniversity Putra Malaysia43400 UPMSerdangSelangorMalaysia BRE Centre for Innovative Construction Materials Department of Architecture and Civil EngineeringUniversity of BathBathBA2 7AYUK

Olivine sand is a natural mineral,which,when added to soil,can improve the soil’s mechanical properties while also sequester carbon dioxide(CO2)from the surrounding *** originality of this paper stems from the novel two-stage *** the first stage,natural carbonation of olivine and carbonation of olivine treated soil under different CO2pressures and times were *** this stage,the unconfined compression test was used as a tool to evaluate the strength *** the second stage,details of the installation and performance of carbonated olivine columns using a laboratory-scale model were *** this respect,olivine was mixed with the natural soil using the auger and the columns were then carbonated with gaseous *** unconfined compressive strengths of soil in the first stage increased by up to 120% compared to those of the natural untreated *** strength development was found to be proportional to the CO2pressure and carbonation *** analyses indicated the presence of magnesite on the surface of carbonated olivinetreated soil,demonstrating that modified physical properties provided a stronger and stiffer *** performance of the carbonated olivine-soil columns,in terms of ultimate bearing capacity,showed that the carbonation procedure occurred rapidly and yielded a bearing capacity value of 120 k *** of this study are of significance to the construction industry as the feasibility of carbonated olivine for strengthening and stabilizing soil is *** applicability lies in a range of different geotechnical applications whilst also mitigates the global warming through the sequestration of CO2.

关键词： Olivine Soil stabilisation CO_2 deposition Climate change Unconfined compressive strength Microstructure analysis

The dynamics of the piglet gut microbiome during the weaning transition in association with health and nutrition

在线全文

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2018年第4期9卷 971-979页

作者： Robin B.Guevarra Sang Hyun Hong Jin Ho Cho Bo-Ra kim Jiwon Shin Jun Hyung Lee Bit Na Kang Young Hwa kim Suphot Wattanaphansak richard E.Isaacson Minho Song Hyeun Bum kim Department of Animal Resources Science Dankook UniversityCheonanSouth Korea Division of Food and Animal Sciences Chungbuk National UniversityCheongjuSouth Korea Abbvie Bioresearch Center AbbvieWorcesterMAUSA National Institute of Animal Science Rural Development AdministrationWanjuSouth Korea Department of Veterinary Medicine Faculty of Veterinary ScienceChulalongkorn UniversityPathum WanBangkok 10330Thailand Department of Veterinary and Biomedical Sciences University of MinnesotaSt.PaulMN 55108USA Division of Animal and Dairy Science Chungnam National UniversityDaejeonSouth Korea

Background: Understanding the composition of the microbial community and its functional capacity during weaning is important for pig production as bacteria play important roles in the pig’s health and growth performance. However,limited information is available regarding the composition and function of the gut microbiome of piglets in ***, we performed 16 S rRNA gene and whole metagenome shotgun sequencing of DNA from fecal samples from healthy piglets during weaning to measure microbiome shifts, and to identify the potential contribution of the early-life microbiota in shaping piglet health with a focus on microbial stress responses, carbohydrate and amino acid ***: The analysis of 16 S rR NA genes and whole metagenome shotgun sequencing revealed significant compositional and functional differences between the fecal microbiome in nursing and weaned piglets. The fecal microbiome of the nursing piglets showed higher relative abundance of bacteria in the genus Bacteroides with abundant gene families related to the utilization of lactose and galactose. Prevotel a and Lactobacil us were enriched in weaned piglets with an enrichment for the gene families associated with carbohydrate and amino acid metabolism. In addition, an analysis of the functional capacity of the fecal microbiome showed higher abundances of genes associated with heat shock and oxidative stress in the metagenome of weaned piglets compared to nursing ***: Overal, our data show that microbial shifts and changes in functional capacities of the piglet fecal microbiome resulted in potential reductions in the effects of stress, including dietary changes that occur during *** results provide us with new insights into the piglet gut microbiome that contributes to the growth of the animal.

关键词： Metagenomics Microbiome Piglets 16S rRNA Weaning

Mass Spectrometric Analyses Reveal a Central Role for Ubiquitylation in Remodeling the Arabidopsis Proteome during Photomorphogenesis

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2017年第6期10卷 846-865页

作者： Victor Aguilar-Hernandez Do-Young kim Robert J. Stankey Mark Scalf Lloyd M. Smith richard D. Vierstra Department of Biology Washington University in St. Louis Campus Box 1137 One Brookings Drive St. Louis MO 63130 USA Department of Genetics 425-G Henry Mall Department of Chemistry 1101 University Avenue University of Wisconsin-Madison Madison WI 53706 USA Advanced Bio Convergence Center Pohang Technopark Gyeong-Buk South Korea 37668

The switch from skotomorphogenesis to photomorphogenesis is a key developmental transition in the life of seed plants. While much of the underpinning proteome remodeling is driven by light-induced changes in gene expression, the proteolytic removal of specific proteins by the ubiquitin-26S proteasome system is also likely paramount. Through mass spectrometric analysis of ubiquitylated proteins affinity-purified from etiolated Arabidopsis seedlings before and after red-light irradiation, we identified a number of influential proteins whose ubiquitylation status is modified during this switch. We observed a substantial enrichment for proteins involved in auxin, abscisic acid, ethylene, and brassinosteroid signaling, peroxisome function, disease resistance, protein phosphorylation and light perception, including the phytochrome （Phy） A and phototropin photoreceptors. Soon after red-light treatment, PhyA becomes the dominant ubiquitylated species, with ubiquitin attachment sites mapped to six lysines. A PhyA mutant protected from ubiquitin addition at these sites is substantially more stable in planta upon photoconversion to Pfr and is hyperactive in driving photomorphogenesis. However, light still stimulates ubiquitylation and degradation of this mutant, implying that other attachment sites and/or proteolytic pathways exist. Collectively, we expand the catalog of ubiquitylation targets in Arabidopsis and show that this post- translational modification is central to the rewiring of plants for photoautotrophic growth.

关键词： Arabidopsis ubiquitin mass spectrometry photomorphogenesis phytochrome degradation