Contaminated open fracture and crush injury: a murine model

作     者：Shawn R Gilbert Justin Camara Richard Camara Lynn Duffy Ken Waites Hyunki Kim Kurt Zinn 

作者机构：Department of SurgeryUniversity of Alabama at Birmingham AL USA Loma Linda School of MedicineLoma LindaCAUSA Departments of PathologyUniversity of Alabama at BirminghamAL USA Department of RadiologyUniversity of Alabama at BirminghamAL USA 

出 版 物：《Bone Research》 (骨研究（英文版）)

年 卷 期：2015年第3卷第1期

页      面：31-39页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：Department of Defense Congressionally Directed Medical Research Program OR 090206 to SG.The Small Animal Phenotyping Core provided faxiotron and micro CT imaging (P30DK056336 and P30DK079626) 

主　　题：MRSA Contaminated open fracture and crush injury a murine model 

摘      要：Modern warfare has caused a large number of severe extremity injuries, many of which become infected. In more recent conflicts, a pattern of co-infection with Acinetobacter baumannii and methicillin-resistant Staphylococcus aureus has emerged. We attempted to recreate this pattern in an animal model to evaluate the role of vascularity in contaminated open fractures. Historically, it has been observed that infected bones frequently appear hypovascular, but vascularity in association with bone infection has not been examined in animal models. Adult rats underwent femur fracture and muscle crush injury followed by stabilization and bacterial contamination with A. baumannii complex and methicillin-resistant Staphylococcus *** and perfusion were assessed by micro CT angiography and SPECT scanning, respectively, at 1, 2 and 4 weeks after injury. Quantitative bacterial cultures were also obtained. Multi-bacterial infections were successfully created, with methicillin-resistant S. aureus predominating. There was overall increase in blood flow to injured limbs that was markedly greater in bacteria-inoculated limbs. Vessel volume was greater in the infected group. Quadriceps atrophy was seen in both groups, but was greater in the infected group. In this animal model, infected open fractures had greater perfusion and vascularity than non-infected limbs.