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Landscape genetics reveals inbreeding and genetic bottlenecks in the extremely rare short-globose cacti Mammillaria pectinifera (Cactaceae) as a result of habitat fragmentation

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Plant Diversity 2017年第1期39卷 13-19页

作者： reyna maya-garcia Santiago Arizaga Pablo Cuevas-Reyes Juan Manuel Penaloza-Ramirez Victor Rocha Ramirez Ken Oyama Facultad de Biologia Universidad Michoacana de San Nicolas de Hidalgo Mexico Escuela Nacional-de Estudios Superiores Unidad Morelia UNAM Antigua Carretera a Pdtzcuaro 8701 Colonia Ex-Hacienda de SanJose de la Huerta58190 Morelia Michoacan Mexico

Mammillaria pectinifera is an endemic, short-globose cactus species, included in the IUCN list as a threatened species with only 18 remaining populations in the Tehuacfin-Cuicatlan Valley in central Mexico. We evaluated the population genetic diversity and structure, connectivity, recent bottlenecks and population size, using nuclear microsatellites. M. pectinifera showed high genetic diversity but some evidence of heterozygote deficiency （F15）, recent bottlenecks in some populations and reductions in population size. Also, we found low population genetic differentiation and high values of connectivity for M. pectinifera, as the result of historical events of gene flow through pollen and seed dispersal. M. pectinifera occurs in sites with some degree of disturbance leading to the isolation of its populations and decreasing the levels of gene flow among them. Excessive deforestation also changes the original vegetation damaging the natural habitats. This species will become extinct if it is not properly preserved. Furthermore, this species has some ecological features that make them more vulnerable to disturbance such as a very low growth rates and long life cycles. We suggest in situ conservation to prevent the decrease of population sizes and loss of genetic diversity in the natural protected areas such as the Tehuacan-Cuicatlan Biosphere Reserve. In addition, a long-term ex situ conservation program is need to construct seed banks, and optimize seed germination and plant establishment protocols that restore disturbed habitats. Furthermore, creating a supply of living plants for trade is critical to avoid further extraction of plants from nature.

关键词： MammiUaria pectiniferaNuclear SSRGenetic diversityRecent bottlenecksEffective population size

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New Rotative Radiofrequency Technology: A Multicenter Retrospective Study on Efficacy and Safety

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Journal of Cosmetics, Dermatological Sciences and Applications 2024年第1期14卷 69-83页

作者： José Antonio Ferrández Martínez Gregorio Viera Mármol Fabrizio Vignoli Joanet Perera Agustin Blanch Mara Suárez Alicia Robledo Jorge reyna Vargas Lamas Sinclair Barcelona Spain Studio Vignoli Bologna Italy Clinica Perla Medic Alicante Spain Dr. Agustin Blanch Clinica Barcelona Spain Intek Medical Technology S.L.U. Barcelona Spain

Background: Non-ablative radiofrequency (RF) technology is widely used for the treatment of signs of aging, skin laxity, localized fat and cellulite. However, many RF devices suffer from technical limitations that make the results highly dependent on the user’s experience or clinical limitations that compromise safety, efficacy and comfort. Objective: To evaluate the efficacy and safety of a novel semi-automatic non-ablative RF device for skin tightening, body shaping and cellulite reduction. Materials and methods: A retrospective multicenter study was performed using a novel rotative RF device with temperature and impedance control (Sculpt&Shape®, Sinclair, Spain). 58 subjects underwent a minimum of 4 treatments every 1-2 weeks. A total of 120 treatments (56.7% body and 43.3% facial) and 1034 sessions were performed. Efficacy was evaluated by 3 researchers using the Global Aesthetic Improvement Scale (GAIS) and anthropometric measurements were collected. Subject and practitioner satisfaction were recorded. Safety was evaluated by analyzing the perception of pain during the treatment and the side and adverse effects immediately after treatment and over the next 48 hours. Results: Between 25% and 50% improvement was achieved according to the GAIS. Statistically significant (p < 0.01) abdominal fold reduction of 5.1 mm and circumference reduction of 32.0 mm, 16.4 mm and 13.9 mm were recorded for the abdomen, legs and arms, respectively. 77.5% of subjects and 94.9% of practitioners were either satisfied or very satisfied. Minor adverse effects were found in 0.97% of the sessions. Conclusion: This novel semi-automatic rotative RF device has been found to provide treatments that are effective, safe and comfortable for both practitioners and subjects. .

关键词： Rotative Radiofrequency RF Cellulite Skin Tightening Body Shaping

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Bioorthogonal chemistry:a covalent strategy for the study of biological systems

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Science China Chemistry 2010年第1期53卷 61-70页

作者： LIM reyna K.V. Department of Chemistry State University of New York at Buffalo

The development of genetically encoded,wavelength-tunable fluorescent proteins has provided a powerful imaging tool to the study of protein dynamics and functions in cellular and organismal ***,many biological functions are not directly encoded in the protein primary sequence,e.g.,dynamic regulation afforded by protein posttranslational modifications such as *** meet this challenge,an emerging field of bioorthogonal chemistry has promised to offer a versatile strategy to selectively label a biomolecule of interest and track their dynamic regulations in its native *** strategy has been successfully applied to the studies of all classes of biomolecules in living systems,including proteins,nucleic acids,carbohydrates,and *** the incorporation of a bioorthogonal reporter site-selectively into a biomolecule through either genetic or metabolic approaches has been well established,the development of bioorthogonal reactions that allow fast ligation of exogenous chemical probes with the bioorthogonal reporter in living systems remains in its early ***,we review the recent development of bioorthogonal reactions and their applications in various biological systems,with a detailed discussion about our own work―the development of the tetrazole-based,photoinducible 1,3-dipolar cycloaddition reaction.

关键词： bioorthogonal reaction proteins biotechnology 1 3-dipolar cycloaddition click chemistry tetrazole

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Use of family structure information in interaction with environments for leveraging genomic prediction models

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The Crop Journal 2020年第5期8卷 843-854页

作者： reyna Persa Hiroyoshi Iwata Diego Jarquin Department of Agronomy and Horticulture University of Nebraska-LincolnLincolnNE 68583USA Graduate School of Agricultural and Life Sciences The University of TokyoBunkyoTokyo 113-8657Japan

The characterization of genomes with great detail offered by the modern genotyping platforms have opened a venue for accurately predicting the genotype-by-environment interaction(GE)effects of untested genotypes in different environmental *** developed statistical models have shown the advantages of including the GE interaction component in the prediction context using molecular markers,pedigree,or *** order to leverage the family information of highly structured populations when pedigree data is not available,we developed a model that uses the family membership *** proposed model extends the reaction norm model by including the interaction between families and environments(FE).A representative fraction of a soybean Nested Association Mapping population(16,187 grain yield records)comprising 38 bi-parental families(1358 genotypes)observed in 18 environments(2011,2012,and 2013)was used to contrast the proposed model with three conventional prediction *** crossvalidation scenarios(prediction of tested[CV2]and untested[CV1]genotypes)with a twofold design(50%for training and testing sets)were used for mimicking prediction situations that breeders face in *** showed that the family factor in interaction with environments explains a sizable amount of the phenotypic *** helped to improve the predictive ability with respect to the main effects model(GBLUP)around 41%(CV2)and 49%(CV1),and about 17%with respect to the conventional reaction norm *** inclusion of the FE term not only improved the global results but also significantly increased the prediction accuracy of those environments where the conventional models showed a very poor *** results show the importance of taking into consideration the family structure existing in breeding programs for improving the selection strategies in multi-parental populations.

关键词： interaction structure instead

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Tentative Identification of Phytochemicals and Antioxidant Activities during Fruit-Ripening on Chamaedorea radicalis Mart.

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Phyton-International Journal of Experimental Botany 2020年第2期89卷 361-373页

作者： Gerardo Montelongo-Ruiz Yolanda del Rocio Moreno-Ramirez Maria Cruz Juarez-Aragon NohemiNino-garcia reyna Ivonne Torres-Acosta Torres-Castillo Jorge Ariel Universidad Autonoma de Tamaulipas Instituto de Ecologia AplicadaCiudad Victoria87019Mexico Universidad Autonoma de Tamaulipas Unidad Academica Multidisciplinaria ManteCiudad Mante89840Mexico

This work aims to determine the phytochemical characterization of the pericarp of Chamaedorea radicalis *** as a non-timber product with potential to obtain phytochemicals with potential applications in the *** from *** were grouped in four ripening stages named as S1,S2,S3 and S4,according to maturity;S1 the most unripe stage and S4 the completely ripe *** of total phenolic compounds,free radical scavenging activities and total flavonoid contents using spectrophotometric methods were ***,the tentative identification of phytochemicals during fruit ripening was done using *** phenolic compound(TPC)content ranged from 7.24 to 12.53 mg gallic acid equivalents per gram of fresh weight(mg GAE/g FW).Total flavonoids(TF)contents ranged from 0.163 to 0.23 mg of quercetin equivalents per g FW(mg QE/g FW).Free radical scavenging activity against DPPH and ABTS radicals varied from 40.80 to 53.68 and from 22.29 to 37.76 mmol Trolox equivalents g FW(mmol TE/g FW),*** assay in vitro by FRAP(ferric reducing antioxidant power)method showed that S3 was the highest level with antioxidant power while S4 was the lowest with Red ripeness stage showed the lowest contents for all *** spectrometry allowed detection of 26 compounds,including phenolics,alkaloids and ***,Kaempferol 3-neohesperidoside and the four saponins identified were present in all ripeness *** phytochemical identification and the spectrophotometric determinations showed that the pericarp of *** presented antioxidants and compounds related to alkaloids,phenolics and *** presence and abundance of each phytochemical regarding each ripeness stage should be considered.

关键词： Metabolite identification palm free radical scavenging phenolic compounds saponins

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Regeneration patterns in Mexican pine-oak forests

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Forest Ecosystems 2019年第4期6卷 384-395页

作者： Teresa Alfaro reyna Jordi Martínez-Vilalta Javier Retana CREAF E-08193 Cerdanyola del VallèsBarcelonaSpain Universitat Autònoma de Barcelona E-08193 Cerdanyola del VallèsBarcelonaSpain

Background: Global change is causing an increase in the incidence of natural and anthropogenic disturbances on forests, which frequently interact synergistically and promote changes in forest structure, composition and functioning. In this study we evaluate the regeneration of Pinaceae and Fagaceae species in pure and mixed stands in Mexico to determine if current regeneration patterns are indicative of changes in the relative dominance of these two tree families, as observed in other temperate regions. We also identify the environmental factors that determine the regeneration patterns of Pinaceae and Fagaceae species in these ***: We use data from two consecutive surveys of the National Inventory of Forests and Soils of Mexico(INFyS),obtained in 2004–2009 and 2009–2014. Our results show that the spatial patterns of regeneration are affected by forest structure, by climate, by the type and intensity of disturbances and by land tenure. Importantly, the presence and abundance of Fagaceae regeneration is generally higher than that of Pinaceae, and tends to be favoured(relative to Pinaceae) under warmer climates and by the presence of ***: The higher regeneration of Fagaceae relative to Pinaceae under warmer and fire-prone conditions could have important impacts on the composition and functioning of Mexican temperate forests under ongoing climate change, as well as affect their resilience to future disturbances.

关键词： Fagaceae Forest dynamics Forest inventory Mexico Pinaceae Regeneration Temperate forests

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First progeria monkey model generated using base editor

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Protein & Cell 2020年第12期11卷 862-865页

作者： Pradeep Reddy Yanjiao Shao reyna Hernandez-Benitez Estrella Nunez Delicado Juan Carlos Izpisua Belmonte Gene Expression Laboratory Salk Institute for Biological Studies10010 North Torrey Pines RoadLa JollaCA 92037USA Universidad Catolica San Antonio de Murcia(UCAM) Campus de los JerdnimosN°13512Guadalupe 30107Spain

In this issue,Wang et *** on the generation of a nonhuman primate model of Hutchinson-Gilford progeria syndrome(HGPS)using a base *** editing is an emerging novel genome editing technique for modifying a single base pair at specific sites in the *** editors(BEs)have two prin cipal comp orients,a catalytically in active or single strand cleaving Cas-variant,which binds to the guide RNA and a nucleobase deaminase domain to convert specific base pairs at the target loci(Komor et al.,2016;Nishida et al.,2016;Gaudelli et al.,2017).Cytosine base editor(CBE)and adenine base editor(ABE)are two baseeditors,which con vert Cytosi ne-Gua nine(C-G)to Thymine-Adenine(T-A)and A-T to G-C,respectively.

关键词： base convert editor

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An internal control applied to RT-PCR detection of HCV and HIV-1 in human pooled plasma and plasma-derived medicinal products

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BIOCELL 2015年第2期39卷 15-23页

作者： Gonzalo RODRÍGUEZ-LOMBARDI Luciana reyna María Susana VITALI Susana GENTI-RAIMONDI Laboratorio de Hemoderivados Universidad Nacional de Córdoba Centro de Investigaciones en Bioquímica Clínica e Inmunología Departamento de Bioquímica ClínicaFacultad de Ciencias QuímicasUniversidad Nacional de Córdoba-Consejo Nacional de Investigaciones Científicas y TécnicasX5000HUA CórdobaArgentina

A competitive internal control(IC)adapted to RT-PCR in-house assay was developed for HCV RNA detection in human pooled ***,it was applied in a multiplex RT-PCR for the HIV-1 and HCV RNA screening in human pooled plasma and plasma-derived products.A 258-bp PCR product from the 5´non-coding region of HCV genome was obtained.A competitive IC template was constructed by inserting a 52-bp double strand sequence into the NheI site of the 258-bp *** sequence was cloned and the obtained plasmid was used to generate a synthetic *** IC/RNA was incorporated in in-house HCV and/or HIV PCR technique to monitor the efficiency of extraction,reverse transcription,and PCR amplification *** was also used to detect all major genotypes of HCV and HIV-1 strains with similar *** detection limit of the assay for HCV and HIV-1 was 52.7 IU/mL and 164.2 IU/mL,*** techniques have been evaluated in international programs of external quality assurance with highly satisfactory *** IC is an essential reagent in PCR techniques to detect and identify HCV and HIV-1 in pooled plasma samples involved in the manufacture of plasma-derived products as well as in the field of clinical microbiology with limited resources.

关键词： HCV HIV internal control RT-PCR

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Rheumatic manifestations of inflammatory bowel disease

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World Journal of Gastroenterology 2009年第44期15卷 5517-5524页

作者： Tatiana Sofía Rodríguez-reyna Cynthia Martínez-Reyes Jesús Kazúo Yamamoto-Furusho Department of Immunology and Rheumatology National Institute of Medical Sciences and Nutrition Salvador ZubiránVasco de Quiroga 15Col Sección XVI. DelTlalpanMexico City 14000Mexico Department of Gastro-enterology National Institute of Medical Sciences and Nutrition Salvador ZubiránVasco de Quiroga 15Col Sección XVI. DelTlalpanMexico City 14000Mexico

This article reviews the literature concerning rheu-matic manifestations of inflammatory bowel disease (IBD),including common immune-mediated pathways,frequency,clinical course and therapy. Musculoskel-etal complications are frequent and well-recognized manifestations in IBD,and affect up to 33% of pa-tients with IBD. The strong link between the bowel and the osteo-articular system is suggested by many clinical and experimental observations,notably in HLA-B27 transgenic rats. The autoimmune pathogenic mechanisms shared by IBD and spondyloarthropathies include genetic susceptibility to abnormal antigen pre-sentation,aberrant recognition of self,the presence of autoantibodies against specific antigens shared by the colon and other extra-colonic tissues,and increased intestinal permeability. The response against microor-ganisms may have an important role through molecular mimicry and other mechanisms. Rheumatic mani-festations of IBD have been divided into peripheral arthritis,and axial involvement,including sacroiliitis,with or without spondylitis,similar to idiopathic anky-losing spondylitis. Other periarticular features can oc-cur,including enthesopathy,tendonitis,clubbing,peri-ostitis,and granulomatous lesions of joints and *** and osteomalacia secondary to IBD and iatrogenic complications can also occur. The manage-ment of the rheumatic manifestations of IBD consists of physical therapy in combination with local injection of corticosteroids and nonsteroidal anti-inflammatory drugs; caution is in order however,because of their possible harmful effects on intestinal integrity,perme-ability,and even on gut inflammation. Sulfasalazine,methotrexate,azathioprine,cyclosporine and lefluno-mide should be used for selected indications. In some cases,tumor necrosis factor-α blocking agents should be considered as first-line therapy.

关键词： Inflammatory bowel disease Spondylitis Rheumatic diseases

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Long-term trends in a forest ungulate community: park establishment increases numbers, but poaching is a constant threat

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Zoological Research 2021年第2期42卷 207-211页

作者： Rong Hou Rafael reyna-Hurtado Patrick Omeja Charles Tumwesigye Dipto Sarkar Jan F.Gogarten Colin A.Chapman Department of Anthropology McGill UniversityMontrealQC H3A 2A7Canada Shaanxi Key Laboratory for Animal Conservation Northwest UniversityXi’anShaanxi 710069China El Colegio de la Frontera Sur ECOSURCampecheMéxico Makerere University Biological Field Station Fort PortalUganda Uganda Wildlife Authority PO Box 3530Kampala 24500Uganda Department of Geography and Environmental Studies Carleton UniversityOttawa M5B 2K3Canada Viral Evolution and Epidemiology of Highly Pathogenic Microorganisms Robert Koch InstituteSeestraße 1013353BerlinGermany School of Life Sciences University of KwaZulu-NatalPietermaritzburgSouth Africa Department of Anthropology Center for the Advanced Study of Human PaleobiologyGeorge Washington UniversityWashington DC 20052USA

DEAR EDITOR Deforestation represents one of the greatest threats to tropical forest mammals, and the situation is greatly exacerbated by bushmeat hunting. To construct informed conservation plans,information must be gathered about responses to habitat degradation, regeneration, and hunting over a sufficiently long period to allow demographic responses.

关键词： forest habitat threat

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