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Mortalin imaging in normal and cancer cells with quantum dot immuno conjugates

Cell Research 2003年第6期13卷 503-507页

作者： ZEENIA KAUL,TOMOKO YAGUCHI,SUNIL C KAUL,TAKASHI HIRANO,renu wadhwa,KAZUNARI TAIRA National Institute of Advanced Industrial Science and Technology (AIST) 1-1-1 Higashi Tsukuba Ibaraki3058562 Japan National Institute of Advanced Industrial Science and Technology (AIST) 1-1-1 Higashi Tsukuba Ibaraki3058562 Japan National Institute of Advanced Industrial Science and Technology (AIST) 1-1-1 Higashi Tsukuba Ibaraki3058562 Japan National Institute of Advanced Industrial Science and Technology (AIST) 1-1-1 Higashi Tsukuba Ibaraki3058562 Japan National Institute of Advanced Industrial Science and Technology (AIST) 1-1-1 Higashi Tsukuba Ibaraki3058562 Japan National Institute of Advanced Industrial Science and Technology (AIST) 1-1-1 Higashi Tsukuba Ibaraki3058562 Japan

Quantum dots are the nanoparticles that are recently emerging as an alternative to organic fluorescence probes in cell biology and biomedicine, and have several predictive advantages. These include their i) broad absorption spectra allowing visualization with single light source, ii) exceptional photo-stability allowing long term studies and iii) narrow and symmetrical emission spectrum that is controlled by their size and material composition. These unique properties allow simultaneous excitation of different size of quantum dots with a single excitation light source, their simultaneous resolution and visualization as different colors. At present there are only a few studies that have tested quantum dots in cellular imaging. We describe here the use of quantum dots in mortalin imaging of normal and cancer cells. Mortalin staining pattern with quantum dots in both normal and cancer cells mimicked those obtained with organic florescence probes and were considerably stable.

关键词： quantum dots immunofluorescence mortalin visualization.

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Designed fabrication of active tumor targeting covalent organic framework nanotherapeutics via a simple post-synthetic strategy

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Nano Research 2023年第5期16卷 7085-7094页

作者： Yue Yu Guoxin Zhang Zhongping Li Jia Wang Yang Liu Rahul Bhardwaj renu wadhwa Yuki Nagao Mototada Shichiri Ran Gao Biomedical Research Institute National Institute of Advanced Industrial Science and Technology(AIST)Osaka 563-8577Japan AIST-INDIA DAILAB DBT-AIST International Center for Translational&Environmental Research(DAICENTER)AISTTsukubaIbaraki 305-8565Japan Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases NHC Key Laboratory of Human Disease Comparative MedicineNational Human Diseases Animal Model Resource CenterInstitute of Laboratory Animal ScienceChinese Academy of Medical Sciences(CAMS)&Peking Union Medical CollageBeijing 100021China School of Materials Science Japan Advanced Institute of Science and TechnologyIshikawa 923-1211Japan Department of Chemical and Biomolecular Engineering Yonsei University50 Yonsei-roSeodaemun-guSeoul 120-749Republic of Korea Flexible 3D System Integration Laboratory SANKENOsaka UniversityOsaka 567-0047Japan

Developing agents that can accurately differentiate tumors from normal healthy tissues is of utmost importance for safe cancer *** targeting has been considered as an effective technique for tumor *** this work,we demonstrate a folate-functionalized nanoscale covalent organic framework(FATD nCOF)highly specific to cancer cells through active targeting of their enriched folate receptors(FRs).The FATD nCOF prepared by simple post-synthetic modification of the COF surface defeats disperses well in water and exhibits a high loading capacity for various anticancer *** biocompatible FATD nCOF is selectively internalized by FR-harboring cancer cells and consequently augments the efficacy of the loaded drug,Withaferin A(Wi-A),for targeted cancer cell *** biomolecular mechanism studies,Wi-A-loaded FATD(FATD@Wi-A)nanocomposites show remarkably a higher rate of apoptosis in FR-enriched cancer *** analyses of FR-positive and FR-negative tumor xenografts reveal enhanced selective antitumor activity of FATD@Wi-A *** together,the study findings suggest that FATD nCOF holds great promise for active targeting of tumors in *** simple yet effective technology might be valuable for creating new state-of-the-art COFs for chemical and biomedical applications.

关键词： covalent organic framework active targeting folate receptor drug delivery cancer therapy

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p53-independent upregulation of p21WAF1 in NIH 3T3 cells malignantly transformed by mot-2

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Cell Research 2001年第1期11卷 55-60页

作者： TAKANO SYUICHI, renu wadhwa, YOUJI MITSUI, SUNIL C KAUL (National Institute of Bioscience and Human-Technology, AIST, 1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan) (Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japa 2Institute of Applied Biochemistry University of Tsukuba Tsukuba Ibaraki 305-8577 Japan 3Chugai Research Institute for Molecular Medicine 153-2 Nagai Niihari-mura Ibaraki 300-41 Japan 2Institute of Applied Biochemistry University of Tsukuba Tsukuba Ibaraki 305-8577 Japan 1National Institute of Bioscience and Human-Technology AIST 1-1 Higashi Tsukuba Ibaraki 305-8566 Japan

Mot-2 protein is shown to interact with p53 and inhibit its transcriptional activation function. Mot-2 overexpressing stable clones of NIH 3T3 cells were malignantly transformed, however, they had a high level of expression of a p53 downstream gene, p21WAF1. The present study was undertaken to elucidate possible molecular mechanism(s) of such upregulation. An inCreased level of p21WAF1, expression was detected in sta- ble transfectants although an exogenous reporter gene driven by p21WAF1, promoter exhibited lower activity in these cells suggesting that some post-transcriptional mechanism contributes to upregulation. Western analyses of transient and stable clones revealed that upregulation of p21WAF1, in stable NIH 3T3/mot-2 cells may be mediated by cyclin D1 and cdk-2.

关键词： Mouse fibroblasts malignant transformation mot-2 p53 p21WAF1 p16INK4a cyclin D1 cdk-2.

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NIH 3T3 cells malignantly transformed by mot-2 showinactivation and cytoplasmic sequestration of the p53protein

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Cell Research 1999年第4期9卷 261-269页

作者： WADHIVA renu SYIJICHI TAKANO YOUJIMITSUI SUNIL C KAUL( Chugai Research Institute for Molecular Medicine,153-2 Nagai, Niihari-Mura, Ibaraki 300-41, Japan)( National Institute of Bioscience and Hunan-Technology, AIST, 1-1 Higashi, Tsukusa, Ibaraki 305-85 National Institute of Bioscience and Human-Techno logy AIST 1-1 Higashi Tsukuba Ibaraki 305-8566 Japan

INTRODUCTIONInvolvementandinactivationofp53,atumorsuppressorandcellulartranscriptionfactorincellularimmortalizationhasbeenwelldocumented[1-4].Variousactivitiesofp53proteininvolveitsfunctionaldomains,viz.,anamino--term... 详细信息

关键词： NIH 3T3 mot-2 p53 inactivation malignant transformation.

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