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Journal of Biophysical Chemistry 2011年第3期2卷 222-225页

作者： renner de souza leite Wilson Franco Heloisa Sobreiro Selistre-de-Araujo Unidade Academica de Saude do Centro de Educacao e Saude Universidade Federal de Campina GrandeCuiteParaíbaBrazil departamento de Ciencias Fisiologicas Universidade Federal de Sao CarlosSao CarlosBrazil

ACLMT is a Lys49-phospholipase A2 myotoxin isolated from the venom of the Agkistrodon contortrix laticinctus snake. This study investigated the mechanisms involved in effect of ACLMT on membrane water permeability by examining the role of extracellular calcium and strontium in this effect. Water flow across the membrane was gravimetrically measured in bladder sac preparations. The decrease in extracellular calcium promoted a higher response of epithelium to ACLMT, suggesting that the extracellular calcium protects the membrane from the action of the toxin. No alteration in the effect of the toxin on water transport was observed when calcium was replaced by strontium, indicating that this effect is independent of its enzymatic activity. These findings may bring an important contribution towards the comprehension of the mechanisms involved in the effect of Lys49-phospholipase A2 myotoxins on water permeability of epithelial membranes, with implications for the understanding of renal toxicity.

关键词： Epithelial Tissue Membrane Permeability Myotoxin Lys49-Phospholipase A_(2) Toad Bladder Water Transport

Evolutionary fingerprint, phylogenetic and forest structure of tropical montane Atlantic cloud forests along an elevation gradient

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Journal of Mountain Science 2024年第4期21卷 1259-1271页

作者： Ravi Fernandes MARIANO Vanessa leite REZENde Cléber Rodrigo de souza Patrícia Vieira POMPEU Rubens Manoel dos SANTOS Carolina Njaime MENdeS Aloysio souza de MOURA Felipe Santana MACHADO Warley Augusto Caldas CARVALHO Marco Aurélio leite FONTES department of Forest Sciences Federal University of LavrasLavras 37200-900Brazil 2Environmental defence Association of Minas Gerais Belo Horizonte 30360-110Brazil department of Ecology and Conservation Federal University of LavrasLavras 37200-900Brazil department of Forest Sciences State University of Mato Grosso do SulAquidauana 79200-000Brazil Agência Zetta Federal University of LavrasLavras 37200-900Brazil. department of Natural Sciences Federal University of São João del-ReiSão João del-Rei 36307-352Brazil.

Environmental conditions can change markedly over geographical distances along elevation gradients,making them natural laboratories to study the processes that structure *** work aimed to assess the influences of elevation on Tropical Montane Cloud Forest plant communities in the Brazilian Atlantic Forest,a historically neglected *** evaluated the phylogenetic structure,forest structure(tree basal area and tree density)and species richness along an elevation gradient,as well as the evolutionary fingerprints of elevation-success on phylogenetic lineages from the tree *** do so,we assessed nine communities along an elevation gradient from 1210 to 2310 m *** large elevation *** relationships between elevation and phylogenetic structure,forest structure and species richness were investigated through Linear *** occurrence of evolutionary fingerprint on phylogenetic lineages was investigated by quantifying the extent of phylogenetic signal of elevation-success using a genus-level molecular *** results showed decreased species richness at higher elevations and independence between forest structure,phylogenetic structure and *** also verified that there is a phylogenetic signal associated with elevation-success by *** concluded that the elevation is associated with species richness and the occurrence of phylogenetic lineages in the tree communities evaluated in Mantiqueira *** the other hand,elevation is not associated with forest structure or phylogenetic ***,closely related taxa tend to have their higher ecological success in similar ***,we highlight the fragility of the tropical montane cloud forests in the Mantiqueira Range in face of environmental changes(*** warming)due to the occurrence of exclusive phylogenetic lineages evolutionarily adapted to environmental conditions(*** temperature)associated with each elevation range.

关键词： Atlantic Forest Cloud forest Conservation Community ecology Montane forests Phylogenetic diversity Phylogenetic signal

The role of extracellular calcium in the effect of a snake venom Lys49-phospholipase A2 on water transport across epithelial membranes

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Multi-objective forest harvesting under sustainable and economic principles

Journal of Biophysical Chemistry（生物物理化学） 2011年第3期

作者： renner de souza leite Wilson Franco Heloísa Sobreiro Selistre-de-Araujo

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林业研究:英文版 2023年第5期34卷 1379-1394页

作者： Talles Hudson souza Lacerda Luciano Cavalcante de Jesus Franca Isáira leite e Lopes Sammilly Lorrayne souza Lacerda Evandro OrfanóFigueiredo Bruno Henrique Groenner Barbosa Carolina souza Jarochinski e Silva Lucas Rezende Gomide Suzano SA Sao JoséDos CamposSao PauloBrazil Institute of Agricultural Sciences Federal University of Uberlandia–UFUMonte CarmeloMinas GeraisBrazil department of Forest Sciences Federal University of Lavras–UFLALavrasMinas GeraisBrazil Brazilian Agricultural Research Corporation–EMBRAPA Rio BrancoAcreBrazil department of Systems and Automation Federal University of Lavras–UFLALavrasMinas GeraisBrazil

Selective logging is well-recognized as an effective practice in sustainable forest ***,the ecological efficiency or resilience of the residual stand is often in *** time depends on operational variables,diversity,and forest *** logging is excellent but is open to *** may be resolved by mathematical programming and this study integrates the economic-ecological aspects in multi-objective function by applying two evolutionary *** function maximizes remaining stand diversity,merchantable logs,and the inverse of distance between trees for harvesting and log landings *** Brazilian rainforest database(566 trees)was used to simulate our 216-ha *** log landing design has a maximum volume limit of 500 *** nondominated sorting genetic algorithm was applied to solve the main optimization *** parallel,a sub-problem(p-facility allocation)was solved for landing allocation by a genetic *** frontier analysis was applied to distinguish the gradientsα-economic,β-ecological,andγ-*** expected,the solutions have high diameter changes in the residual stand(average removal of approximately 16 m^(3) ha^(-1)).All solutions showed a grouping of trees selected for harvesting,although there was no formation of large clearings(percentage of canopy removaldemographic rates of the remaining *** methodology should support projects of reduced impact logging by using spatial-diversity information to guide better practices in tropical forests.

关键词： Amazon rainforest management Computational intelligence Multi-objective functions Evolutionary computing

Ingredients from integral valorization of Isabel grape to formulate goat yogurt with stimulatory effects on probiotics and beneficial impacts on human colonic microbiota in vitro

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Food Science and Human Wellness 2023年第4期12卷 1331-1342页

作者： Francyeli Araújo Silva Rita de Cássia Ramos do Egypto Queiroga Evandro leite de souza Glenise Bierhalz Voss Maria Manuela Estevez Pintado Margarida Angélica da Silva Vasconcelos department of Nutrition Federal University of PernambucoRecife 50670-420Brazil department of Nutrition Health Sciences CenterFederal University of ParaíbaJoão Pessoa 58050-585Brazil Universidade Católica Portuguesa CBQF-Centro de Biotecnologia e Química Fina-Laboratório AssociadoEscola Superior de BiotecnologiaPorto 4169-005Portugal

Isabel grape(IG)products have high contents of phenolic compounds and fiber recognized for their positive impacts on microorganisms associated with health benefits to *** study evaluated the effects of goat yogurts formulated with ingredients from IG integral valorization on the growth and metabolism of different probiotic strains,as well as on the population of selected bacterial groups and metabolic activity of human colonic microbiota in *** yogurts with IG ingredients(IGI)stimulated the growth of tested Lactobacillus and Bifidobacterium probiotic strains during a 48-h cultivation,as well as decreased the pH values and enhanced the organic acid *** yogurts with IGI increased the population of Lactobacillus *** Bifidobacterium *** a 24-h in vitro colonic fermentation.A stable Firmicutes:Bacteroidetes ratio close to 1 was found in media with goat yogurt formulations during the colonic fermentation,being similar to the effect caused by *** yogurt formulations with IGI caused increased production of short-chain fatty acids and sugar consumption during colonic *** yogurts with IGI should be a valuable strategy for development of novel added-value foods with beneficial effects on gut microbiota and human health.

关键词： Goat dairy product Grape Beneficial microorganisms Gut microbiota Modulatory effects

Role of lysophosphatidic acid and its receptors in health and disease:novel therapeutic strategies

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Signal Transduction and Targeted Therapy 2021年第3期6卷 630-647页

作者： Luiz Henrique Medeiros Geraldo Tania Cristina leite de Sampaio Spohr Rackele Ferreira do Amaral Anna Carolina Carvalho da Fonseca Celina Garcia Fabio de Almeida Mendes Catarina Freitas Marcos Fabio dosSantos Flavia Regina souza Lima Institute de Ciencias BiomedicasUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil Universite de Paris PARCCINSERMF-75015 ParisFrance Institute Estadual do Cerebro Paulo Niemeyer-Secretaria de Estado de Saude Rio de JaneiroBrazil Instituto de Saiide de Nova Friburgo Universidade Federal Fluminense28625-650 Nova FriburgoBrazil

Lysophosphatidic acid(LPA)is an abundant,bioactive phospholipid,with multiple functions both in development and in pathological ***,we review the literature about the differential signaling of LPA through its specific receptors,which makes this lipid a versatile signaling *** differential signaling is important for understanding how this molecule can have such diverse effects during central nervous system development and angiogenesis;and also,how it can act as a powerful mediator of pathological conditions,such as neuropathic pain,neurodegenerative diseases,and cancer ***,we review the preclinical and clinical uses of Autotaxin,LPA,and its receptors as therapeutic targets,approaching the most recent data of promising molecules modulating both LPA production and *** review aims to summarize the most update knowledge about the mechanisms of LPA production and signaling in order to understand its biological functions in the central nervous system both in health and disease.

关键词： therapeutic clinical diseases

Small-scale edaphic heterogeneity as a floristic-structural complexity driver in Seasonally Dry Tropical Forests tree communities

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Journal of Forestry Research 2020年第6期31卷 2347-2357页

作者： Cle´ber Rodrigo de souza Jean Daniel Morel Alisson Borges Miranda Santos Wilder Bento da Silva Vinı´cius Andrade Maia Polyanne Aparecida Coelho Vanessa leite Rezende Rubens Manoel dos Santos Forest Sciences departament Federal University of LavrasCx.P.3037Lavras 37200-000Brazil Biology department Federal University of LavrasCx.P.3037Lavras 37200-000Brazil

Our work aimed to test the hypothesis that soil microscale heterogeneity act as a community ecological driver,increasing diversity and promoting structural shifts on the Seasonally Dry Tropical Forest(SDTF)tree *** evaluated the relationship between microscale edaphic variations and floristic–structural patterns of tree communities in a SDTF fragment located in the southern end of the Brazilian Caatinga *** and soil data were obtained through 27 sample units of 400 m2(20 m 920 m),within each one we measured and identified at species level all arboreal individuals with Circumference at the Breast Height greater or equal to 10 cm,and also collected the soil *** the data we evaluated soil variation influence on the tree community structural and floristic patterns trough generalized linear *** explained the small-scale structural and floristic variations,contributing significantly to biomass,sprouting and to floristic relationships between sample *** was also observed a possible relation of the result with the Caatinga domain biogeographic history,due the presence of Sedimentary Caatinga species,which are not expected for the study *** plays an important role in driving small-scale complexity and diversity of SDTF,but we also suggest that Caatinga biogeographic events have influence on the high heterogeneity patterns.

关键词： Caatinga domain Edaphic variables Sprouting Sedimentary Caatinga

Association of TNFA(-308G/A),IFNG(+874 A/T)and IL-10(-819 C/T)polymorphisms with protection and susceptibility to dengue in Brazilian population

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Asian Pacific Journal of Tropical Medicine 2017年第11期10卷 1065-1071页

作者： Ana Caroline Melo dos Santos Edilson leite de Moura Jean Moises Ferreira Alexandre Wendell Araujo de Moura Ailson Darlan Sales Ferreira Rubens Pereira Bezerra Diego de Siqueira Figueiredo Karol Fireman de Farias Tiago Gomes de Andrade Elaine Virginia Martins de souza Figueiredo Molecular Biology and Gene Expression Laboratory Federal University of AlagoasCampus ArapiracaAv Manoel Severino BarbosaBom SucessoArapiracaAL57309-005Brazil Faculty of Medicine Federal University of AlagoasCampus AC SimoesTabuleiro dos MartinsMaceioAlagoasBrazil

Objective: To evaluate gene polymorphisms and their association with susceptibility to ***: A retrospective case-control study was performed with 262 subjects,comprising 78 dengue fever(DF) patients, 49 dengue hemorrhagic fever(DHF) patients and 135 healthy controls. Genotypic and allelic profiles were identified using polymerase chain reaction based in real time and amplification-refractory mutation ***: We observed a protective association of IL-10(-819 C/T) C allele(P = 0.028,OR = 0.56, CI = 0.34–0.91) against DHF, while the C/T(P = 0.047, OR = 2.10,CI = 1.01–4.38) and T/T(P = 0.008, OR = 3.82, CI = 1.38–10.59) genotypes were associated with DHF and DF, respectively. The dominant model TNFA-308 GA + AA(P = 0.043, OR = 0.45, CI = 0.20–1.00) genotypes were found to have protective effect against dengue infection. A protective association among the IFNG(+874 A/T) A/T genotype against DF(P = 0.02, OR = 0.46, CI = 0.24–0.89) and DHF(P = 0.034,OR = 0.43, CI = 0.19–0.95) was observed. When the studied single-nucleotide polymorphism was analyzed in combination, the combination GTA(P = 0.022, OR = 2.95,CI = 1.18–7.41) was statistically significantly associated with susceptibility to DF and the combination GCT(P = 0.035, OR = 0.28, CI = 0.08–0.90) with protection against the development of ***: This research identifies the association of the IFNG(+874 A/T), TNFA(-308 G/A), IL-10(-819 C/T) genotypes as a factor for protection, susceptibility and severity to dengue.

关键词： Immune system IFNG TNFA IL-10 dengue Polymorphism

Helicobacter pylori and EBV in gastric carcinomas:Methylation status and microsatellite instability

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World Journal of Gastroenterology 2010年第3期16卷 312-319页

作者： Adriana Camargo Ferrasi Nídia Alice Pinheiro Silvia Helena Barem Rabenhorst Otávia Luisa Caballero Maria Aparecida Marchesan Rodrigues Fabrício de Carvalho Celso Vieira de souza leite Marcia Valéria Pitombeira Ferreira Marcos Aurélio Pessoa Barros Maria Inês de Moura Campos Pardini Blood Transfusion Center Botucatu Medical SchoolUNESP-Sao Paulo State University department of Pathology-Federal University of Ceara Ludwig Institute of Cancer Research department of Pathology Medical SchoolUNESP-Sao Paulo State University department of Gastroenterology Surgery Medical SchoolUNESP-Sao Paulo State University

AIM:To verify the methylation status of CDH1, DAPK, COX2, hMLH1 and CDKN2A genes and to evaluate their association with Helicobacter pylori (H. pylori)-cagA+ and Epstein Barr virus (EBV) infections in gastric ***: Methylation-specific PCR (MSP) assay was performed in 89 primary gastric carcinomas (intestinal and diffuse types). Microsatellite instability (MSI) analysis was performed using the BAT26 primer set and PCR products were analyzed with the ABI PRISM 3100 Genetic Analyzer using Genescan 3.7 software (Applied Biosystems). detection of H. pylori and genotyping were performed by PCR, using specifi c primers for ureaseC and cagA genes. The presence of EBV was assessed by in situ hybridization. Statistical analyses were performed using the χ2 or Fisher's exact ***: The most frequent hypermethylated gene was COX-2 (63.5%) followed by DAPK (55.7%), CDH1 (51%), CDKN2A (36%) and hMLH1 (30.3%). Intestinal and diffuse adenocarcinomas showed different methylation profiles and there was an association between methylation of E-CDH1 and H. pylori-cagA+ in the intestinal adenocarcinoma type. MSI was correlated with hMLH1 methylation. There was an inverse correlation between DAPK hypermethylation and ***: We found a strong association between CDH1 methylation and H. pylori-cagA+ in intestinal-type gastric cancer, association of MSI and better prognosis and an heterogeneous COX-2 overexpression.

关键词： Gastric cancer Methylation Microsatellite instability Helicobacter pylori Epstein Barr virus

Inter- and intraobserver agreement in computed tomography enterography in inflammatory bowel disease

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World Journal of Gastroenterology 2016年第45期22卷 10002-10008页

作者： Natally Horvat Camila Carlos Tavares Adriana Ribas Andrade Julia Campos Sim?es Cabral Hilton Muniz Le?o Filho Angela Hissae Motoyama Caiado Serli Kiyomi Nakao Ueda André Zonetti de Arruda leite Aytan Miranda Sipahi Manoel de souza Rocha Radiology department Hospital das Clínicas da Faculdade de Medicina da Universidade de S?o Paulo Gastroenterology department Lim 07 Hospital da Clínicas da Faculdade de Medicina da Universidade de S?o Paulo

AIM To evaluate intra- and interobserver agreement in imaging features in inflammatory bowel disease and comparison with fecal calprotectin(FC) *** Our institutional computed tomography enterography(CTE) database was retrospectively queried to identify patients who underwent CTE from January 2014 to June 2015. Patient inclusion criteria were confirmed inflammatory bowel disease(IBD) and FC collected ded abdominal radiologists, with 12 and 3 years of experience analyzed the CTE regarding localization(small bowel, colonic, both, or no disease detected);type of IBD(inflammatory, stenosing, fistulizing, > 1 pattern, or normal); and signs of active disease(present or absent). In 42 of 44 patients evaluated, routine CTE reports were made by one of the readers who reevaluated the CTEs ≥ 6 mo later, to determine the intraobserver agreement. FC was considered a sign of disease activity when it was higher than 250 μg/*** Forty-four patients with IBD(38 with Crohn's disease and 6 with ulcerative colitis) were included. There was a moderate interobserver agreement regarding localization of IBD(k = 0.540), type of disease(k = 0.410) and the presence of active signs in CTE(k = 0.419). There was almost perfect intraobserver agreement regarding localization, type and signs of active disease in IBD. The k values were 0.902, 0.937 and 0.830, respectively. After a consensus between both radiologists regarding inflammatory activity in CTE, we found that 24(85.7%) of 28 patients who were classified with active disease had elevated FC, and six(37.5%) of 16 patients without inflammatory activity in CTE had elevated FC(P = 0.003). The correlation between elevated FC and the presence of active disease in CTE was significant(k = 0.495, P = 0.001).CONCLUSION We found almost perfect intraobserver and moderate interobserve

关键词： Crohn’s disease Ulcerative colitis Computed tomography Fecal calprotectin Inflammatory bowel disease activity