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Autologous transplantation of bone marrow mononuclear cells improved heart function after myocardial infarction

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Acta Pharmacologica Sinica 2004年第7期25卷 30-40页

作者： Guo-sheng LIN1, Jing-jun Lü, Xue-jun JIANG, Xiao-yan LI, Geng-shan LI Department of Cardiology, renmin Hospitalof Wuhan University, Wuhan 43.060, china Department of Cardiology Renmin Hospital of Wuhan University Wuhan 430060 Chin

AIM: To investigate whether autologous transplantation of adult stem cells could improve post-infarcted heart function. METHODS: Bone marrow mononuclear cells (MNCs) were isolated from adult rabbits’ tibias after coronary ligation. These cells were exposed to 5-azacytidine 10 μmol/L for 24 h on the third day of culture. After being labeled with bromodeoxyuridine (BrdU), the cells were auto-transplanted into bordering zone of the infarcted area at 2 weeks after injury. The animals were killed at 3.days, 2 weeks, 1 month, and 2 months after transplantation, respectively. The left ventricular functions, capillary density, and cardiac nerve density were measured and the differentiation of the engrafted cells was determined by immunostaining. RESULTS: BrdU-labeled MNCs were well aligned with the host cardiomyocytes. Parts of them were incorporated into capillary and arteriolar vessel walls. In addition to inducing angiogenic ligands (basic fibroblast growth factor, vascular endothelial growth factor) and imflammation cytokines (interleukin 1-β) during the early period of MNCs implantation, MNCs induced 2.0-fold increase in capillary density as well. Moreover, GAP43.positive and TH-positive nerve density were markedly higher in the MNCs-treated groups than that in the non-treated hearts. Left ventricular ejection fraction, LV+dp/dtmax, and LV-dp/dtmax were 47 %, 67 %, and 55 % in MNCs-treated heart respectively, which was higher than that of the control heart, whereas left ventricular end-diastolic volume, left ventricular end-diastolic diameter, and left ventricular end-diastolic pressure were 45 %, 22 %, and 50 % respectively in MNCs-treated heart, which was lower than that of the control heart at 2 months after cell transplantation. CONCLUSION: Autologous transplantation of MNCs induced angiogenesis and nerve sprouting and improved left ventricular diastolic function.

关键词： autologous transplantation bone marrow cells stem cell left ventricular function angiogenesis nerve regeneration myocardial infarction

Reliability Growth Test Planning and Verification of Commercial Vehicles

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Automotive Innovation 2019年第4期2卷 328-337页

作者： Liu Jicheng Huang Liyan Zhou renmin Markus Volmer Beiqi Foton Motor Co. Ltd.Beijing 102206China

Reliability and durability are two important technical indicators in automobile research and development.A research-and-design and testing organization can increase inherent quality attributes by adopting a systematic approach based on statistical tools and clearly defined *** process affects the design phase,validation through testing,and quality assurance in *** the basis of reliability growth theory and the Duane model,this study established an estimation method for the definition of the target mileage and specific test cycles in reliability growth testing.A construction method for defin-ing test conditions was proposed that adopts the theory of the design of *** simulation was conducted under a variety of typical test conditions including differing operation times,loads,and logistics modes to predict customer use and detect *** cases were then analyzed in *** the same time,a reliability growth prediction model was established on the basis of the initial test data and used for test process tracking and risk control.

关键词： Reliability Duane model Reliability growth prediction model Failure rate

A Microenvironment, Rather than Chemical, Initiates the Cardiomyogenic Differentiation of Marrow Stromal Cells

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Wuhan University Journal of Natural Sciences 2004年第4期9卷 513-521页

作者： LIU Wei-xin 1,2 , SONG Jian 1 , WAN Yu 1, PAN Guo-dong 1 , LIU Yu 1 , CHENG Bang-chang 2 ,CHEN Xi-chang 1 1. School of Medicine, Wuhan University, Wuhan 43.071, Hubei, china 2. renmin Hospital, Wuhan University, Wuhan 43.060, Hubei, china Wuhan University School of Medicine Wuhan Hubei China (GRID:grid.49470.3e) (ISNI:***) Wuhan University Renmin Hospital Wuhan Hubei China (GRID:grid.49470.3e) (ISNI:***) Wuhan University School of Medicine Wuhan Hubei China (GRID:grid.49470.3e) (ISNI:***) Wuhan University Renmin Hospital Wuhan Hubei China (GRID:grid.49470.3e) (ISNI:***)

To investigate the potential of cardiomyogenic differentiation of rat bone marrow stromal cells (MSCs), they were exposed to 5-azacytidine tretments (single/repeat) at varying concentrations (3. 5, 10 μmol/L) and the fates of the cells were analyzed by immunocytochemistry, Western blot and the reporter gene of enhanced cyan fluorescent protein (ECFP) under the control of ventricular myosin light chain 2 (MLC2v) promoter. MSCs were also cocultured with cardiomyocytes for periods up to 16 days, the expression of cardiac myosin heavy chain (MHC) and troponin I (Tn I) proteins was analyzed. After the induction with 5-azacytidine, neither spontaneously beating cells nor myotubes were found; MHC and Tn I proteins were also undetectable and no ECFP-positive MSCs were detected. But when cocultured with cardiomyocytes, spontaneously contracting MSCs were observed and cardiac specific proteins could be detected. The results proved that the novel effects of 5-azacytidine on the cardiomyogenic differentiation of MSCs should be questioned and a direct intercellular communication with cardiomyocytes is necessary for MSCs to differentiate into cardiomyocytes. Key words cardiomyocyte - cell differentiation - stem cell CLC number Q 813.5 - R 542.2+2 Foundation item: Supported by the Natural Science Foundation of Hubei Province (2002AB00150)Biography: LIU Wei-xin (1976-), male, Ph. D candidate, research direction: stem cell and cellular biology.

关键词： cardiomyocyte cell differentiation stem cell

3. virtual reconstruction of the Pleistocene cheetah skull from the Tangshan, Nanjing, china

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Progress in Natural Science:Materials International 2007年第1期17卷 74-79页

作者： DONG Wei, HOU Xinwen, LIU Jinyi, FANG Yingsan, JIN Changzhu and ZHU Qizhi ( Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences, Beijing 100044, china State Key Laboratory of Pattern Recognition, Institute of Automation, Chinese Academy of Sciences, Beijing 100080, china Nanjing Provincial Museum, Nanjing 210016, china Radiology Department, {3. Hospital, Peking University, Beijing 100044, china) [a]Institute of Vertebrate Paleontology and Palecanthropology Chinese Academy of Sciences Beijing China [b]State Key Laboratory of Pattern Recognition Institute of Automation Chinese Academy of Sciences Beijing China [c]Nanjing Provincial Museum Nanjing China [d]Radiology Department Renmin Hospital Peking University Beijing China

The development of computer tomography and image processing has made it possible to establish virtual 3. reconstruction and non-invasive dissection of fossil specimens. We used these methods to reconstruct a virtual 3. skull of a Pleistocene cheetah skull from the Tuozi cave, Tangshan, Nanjing, and virtually dissected it for anatomic studies, and measured the volumes of different parts of the endocranium. The endocranium of the cheetah skull has showed that its frontal sinus is beehive-like, the frontal lobe of cerebra is relatively large but the temporal lobe is relatively small, its cerebral sulcus and gyrus are more complicated than those of the domestic cat, similar to those of the domestic dog, but simpler than those of giant panda, pig, cattle and horse. The technology of virtual 3. reconstruction and non-invasive dissection of fossil specimens can extend the morphological study from the exterior to the interior, and it can also help to study fragile specimens and virtually backup rare and precious specimens.

关键词： CT imaging 3. reconstruction cheetah skull early Pleistocene Tangshan Nanjing.

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薇拉·凯瑟作品中的生物共同体意识

外国文学研究 2009年第2期31卷 71-80页

作者：孙宏中国人民大学外国语学院

有的学者指控薇拉.凯瑟带着"对帝国主义的怀旧之情","为征服大平原撰写一个温良的版本,借以标示其价值观"。其实凯瑟向往的是未遭人类骚扰的大自然。她称三角叶杨为"平原上最美的树木",正是在她的竭力劝阻下,人们放弃了砍伐一片三角叶... 详细信息

有的学者指控薇拉.凯瑟带着"对帝国主义的怀旧之情","为征服大平原撰写一个温良的版本,借以标示其价值观"。其实凯瑟向往的是未遭人类骚扰的大自然。她称三角叶杨为"平原上最美的树木",正是在她的竭力劝阻下,人们放弃了砍伐一片三角叶杨树林的企图。在《露西.盖哈特》一书中女主人公被用斧头砍树的声音惊醒,做出同样的反应。在《啊,拓荒者!》中艾弗人道地对待动物,却被视为"疯子"。在《死神来迎接大主教》里,拉图尔主教在和一个那伐鹤人一道旅行中,学会了印第安人途经一处乡间却不留下任何痕迹的传统。凯瑟将艾弗和那伐鹤人刻画成坚守以生物为中心的伦理准则的典范,借这些边缘人物的一言一行批判了以人类为中心的帝国主义,标示出一种充满活力的生物共同体意识。

关键词：薇拉·凯瑟帝国主义生物共同体

EU's Trade Policy on china within the WTO Framework

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china's Foreign Trade 2011年第5期 12-15页

作者： Ding Kang SCHOOL OF FINANCE,renmin UNIVERSITY OF china SCHOOL OF FINANCE RENMIN UNIVERSITY OF CHINA

china's WTO accession has been ten years,and we have experienced the joy of economic growth and trade disputes, troubles as *** of them made us to rethink the function of the WTO,and how to use the WTO rules to make our interests more secure and *** 2010,china is the world's largest exporter and the world's second largest national *** country now accounts for about 11% of world trade in *** trade with the EU has gone from(?)4 billion in 1978 to(?)3.5 billion in *** EU continues to be china's main export *** 2010 the EU imported goods worth (?)281.9 billion from china. At the same time,china is Europe's fastest growing export *** companies are deeply integrated in Asian production *** than half of china's exports are currently

关键词：世贸组织贸易政策组织框架中国欧盟世界贸易组织 WTO规则贸易争端

Changes of plasma D(-) -lactate, diamine oxidase and endotoxin in patients with liver cirrhosis

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Hepatobiliary & Pancreatic Diseases International 2004年第1期3卷 58-61页

作者： Peng Ruan, Zuo-Jiong Gong and Quan-Rong Zhang Wuhan, china Department of Infectious Diseases, renmin Hospital Wuhan University Medical College, Wuhan 43.060 , china Department of Infectious Diseases Renmin Hospital Wuhan University Jiefang Road Wuhan 430060 China.

BACKGROUND: Plasma D(-)-lactate and diamine oxidase (DAO) can reflect patients' intestinal mucosal condition. We evaluated the changes of plasma D (-)-lactate, DAO and endotoxin activities and their significance in patients with liver cirrhosis. METHODS: Fifty liver cirrhosis patients were enrolled into experimental group and 3. healthy people into control group. The plasma levels of D(-)-lactate, DAO and endo- toxin were detected spectrophotographically. RESULTS: The level of D(-)-lactate was significantly high- er in the experimental group than that in the control group (P<0.01). Significant differences of D (-)-lactate levels were observed in Child-Pugh subgroups of the experimen- tal group (P <0. 01). The level of DAO was significantly higher in the experimental group than that in the control group (P <0.01), but the level of DAO in Child-Pugh sub- group C was significantly lower than that in Child-Pugh subgroup B (P<0.01). The level of endotoxin was signifi- cantly increased in the experimental group except Child Pugh subgroup A (P<0.01). The plasma levels of D(-) lactate, DAO and endotoxin were positively correlated with each other (P<0.01). CONCLUSIONS: The data suggest that both plasma D(-) lactate and DAO activity are sensitive markers for early diagnosis of gut failure and endotoxemia in patients with liver cirrhosis. The impairment of intestinal barrier func- tion may be one of the critical reasons for deterioration of liver cirrhosis.

关键词： liver cirrhosis D(-)-lactate diamine oxidase endotoxin intestinal permeability gut failure

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传统国有企业向现代企业制度的转变

中国人民大学学报 1995年第4期9卷 1-11,125页

作者： The Research Group of Economic Development Report at renmin University of china

政企不分与所有权约束的行政属性所导致的国家在国有资产上的各种权利得不到切实有效的保护是国有产权模糊的集中体现。应循着产权明晰化思路推进企业改革，确立以资产界定权利的规则，根据国有企业不同类型选择不同的改革战略：提供公... 详细信息

政企不分与所有权约束的行政属性所导致的国家在国有资产上的各种权利得不到切实有效的保护是国有产权模糊的集中体现。应循着产权明晰化思路推进企业改革，确立以资产界定权利的规则，根据国有企业不同类型选择不同的改革战略：提供公共产品的国有企业宜选择国有国营模式，垄断性国有企业宜选择国有国控模式，竞争性国有企业宜进行公司化改造，一部分国有中小型企业宜选择民营化战略。竞争性国有企业公司化改造可选择国有资产的四层授权经营模式，其中重要环节是组建控股公司，通过竞价投标方式购买国有股的控股权，准股票是国有资产管理局向控股公司投入资本的凭证，准股息是国家所有权在经济上的实现形式。

关键词：产权国有国控授权经营控股公司准股票

Human Rights of 21st Century From Perspective of Philosophy of Harmony

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The Journal of Human Rights 2003年第5期2卷 37-38页

作者： Zhang Liwen, professor of renmin University of china. renmin University of china

What we call "human rights" literally refers to those rights to which members of the human race are entitled. The concept of value and human rights assume a paramount importance in china when the country is striving to develop by complementing the enforcement of rule by law and the enforcement of rule by virtue. Neither principle can be truly implemented if human rights are not adequately protected. In other words, development and improvement of human rights conditions will guarantee the development and improvement of rule by law and rule by virtue.

关键词： of it In de Human Rights of 21st Century From Perspective of Philosophy of Harmony into for be