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HIV-1 DNA levels in peripheral blood mononuclear cells of patients with HIV-related non-Hodgkin’s lymphoma

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Chinese Medical Journal 2023年第22期136卷 2741-2743页

作者： Zhenyan Wang Jingna Xun Zichen Song Yinzhong Shen Li Liu Jun Chen Tangkai Qi Jianjun Sun Wei Song Yang Tang Shuibao Xu Junyang Yang Bihe Zhao renfang zhang Department of Infection and Immunity Shanghai Public Health Clinical CenterFudan UniversityShanghai 201508China

To the Editor:Human immunodeficiency virus(HIV)patients have an increased risk of non-Hodgkin’s lymphoma(NHL),even in the era of antiretroviral therapy(ART).[1]The specific mechanisms of HIVrelated NHL(HIV-NHL)remain unclear.It is currently thought that HIV causes lymphoma primarily through chronic immune activation and an increased risk of infection with oncoviruses.In addition,HIV may have some other mechanisms that directly contribute to the occurrence of lymphoma.[2]Even with sustained ART,HIV-DNA remains in infected cells,creating a persistent reservoir of HIV infection.It is not known whether HIV reservoirs are involved in the development of HIV-NHL.In the present study,we detected HIV-1 DNA levels in peripheral blood mononuclear cells(PBMCs)and plasma inflammatory cytokines levels in HIV-NHL,and then evaluated their effects on HIV-NHL development and prognosis.

关键词： lymphoma patients sustained

Long-term case-fatality rate of nontuberculous mycobacterial disease in people living with HIV

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Infectious Diseases of Poverty 2022年第1期11卷 47-55页

作者： Jingjing Hu Ling Gu Yueming Shao renfang zhang Tangkai Qi Jianjun Sun Zhenyan Wang Wei Song Yang Tang Jiangrong Wang Shuibao Xu Junyang Yang Yinzhong Shen Li Liu Jun Chen Hongzhou Lu School of Public Health Bengbu Medical CollegeBengbu233000AnhuiChina Department of Infectious Diseases and Immunology Shanghai Public Health Clinical CenterFudan University2901 Caolang RoadJinshan DistrictShanghai201508China Shenzhen Third People’s Hospital The Second Affiliated Hospital of Southern University of Science and TechnologyShenzhen518000China

Background:Few data are available regarding the long-term case-fatality rate(CFR)among people living with HIV(PLWH)with nontuberculous mycobacteria(NTM)disease.The aim of this study is to analyze the long-term CFR in patients with NTM disease and to identify risk factors for their death.Methods:A retrospective cohort study of 379 cases of microbiologically confirmed NTM disease in PLWH was conducted from January 1,2012,to December 31,2020,in Shanghai,China.We used Kaplan-Meier survival analysis and the log-rank test to compare the long-term CFR in patients with disseminated NTM(DNTM)and localized NTM disease.Univariate Cox proportional hazards regression analysis and a stepwise Cox proportional hazards regression model were used to estimate the predictors of long-term CFR.Results:The cohort was followed up for a median of 26 months.The total CFR was 15.7%by one year and increased to 22.6%at 5 years after the diagnosis of NTM disease.The 5-year CFR of PLWH with DNTM was significantly higher than that of PLWH with localized NTM(26.7%vs 19.6%for DNTM and localized NTM disease,respectively).Older age[hazard ratio(HR)=1.04,95%confidence interval(CI):1.02-1.06,P<0.001],comorbidity(HR=2.05,95%CI:1.21-3.49,P<0.01),DNTM(HR=2.08,95%CI:1.17-3.68,P<0.05),and HIV viral load(HR=1.32,95%CI:1.12-1.55,P<0.001)were all independent risk factors for long-term CFR.In the subgroup analysis,time to culture positivity was negatively correlated with CFR in patients with DNTM(HR=0.90,95%CI:0.82-0.98,P<0.05).Conclusions:NTM was associated with a high long-term CFR in PLWH.Further approaches to prevent NTM disease in PLWH are urgently needed.

关键词： HIV/AIDS Nontuberculous mycobacteria Case-fatality rate Risk factor

蛋白质组学分析揭示水稻地上部对缺铁和高铁胁迫的响应

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土壤学报 2024年第1期61卷 118-128页

作者：张欣王若男沈仁芳兰平土壤与农业可持续国家重点实验室(中国科学院南京土壤研究所) 南京210008 中国科学院大学北京100049

铁在矿质土壤中含量丰富,但在中性和碱性土壤中大多以不易被植物吸收利用的氧化物或氢氧化物形式存在;稻田土壤在淹水条件时氧化还原电位较低,大量铁以易被植物吸收利用的亚铁形式存在。土壤中铁的生物有效性过低或过高均会导致植物的... 详细信息

铁在矿质土壤中含量丰富,但在中性和碱性土壤中大多以不易被植物吸收利用的氧化物或氢氧化物形式存在;稻田土壤在淹水条件时氧化还原电位较低,大量铁以易被植物吸收利用的亚铁形式存在。土壤中铁的生物有效性过低或过高均会导致植物的生长发育受阻。本研究对缺铁(0μmol·L^(-1))、铁充足(40μmol·L^(-1))和高铁(350和500μmol·L^(-1))条件生长的水稻地上部进行了非标记蛋白质组学分析。结果显示,与铁充足条件相比,缺铁和两种浓度的高铁胁迫水稻中分别有130、157和118个蛋白质的丰度发生显著变化。基因本体富集分析显示,缺铁和高铁胁迫下的差异蛋白在初级代谢过程、有机氮化合物代谢过程、蛋白质代谢过程和细胞成分组织或生物发生等生物学过程均显著富集;差异蛋白还参与核糖体、光合作用和氧化磷酸化等代谢途径。缺铁胁迫显著影响参与苯丙烷类物质和辅助因子生物合成的蛋白质丰度,而高铁胁迫则引起氨基酸生物合成过程的蛋白质丰度发生显著变化。本研究发掘到一系列可用于水稻铁高效育种工作的候选蛋白,还发现了一些功能未知的差异蛋白可作为后续水稻铁胁迫响应的研究目标,同时为理解植物应对铁胁迫的完整响应网络提供了补充信息。

关键词：铁胁迫蛋白质组光合作用核糖体氧化胁迫氨基酸的生物合成

Antiretroviral therapy-naïve people living with HIV tend to have more severe symptoms of COVID-19

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Chinese Medical Journal 2023年第22期136卷 2753-2755页

作者： Jinfeng Sun Rui Jiang Yueming Shao Jingjing Hu Zhihang Zheng Luling Wu Li Liu Junyang Yang Yinzhong Shen renfang zhang Tangkai Qi Jianjun Sun Zhenyan Wang Yang Tang Wei Song Shuibao Xu Bihe Zhao Jun Chen Department of Infection and Immunology Shanghai Public Health Clinical CenterFudan UniversityShanghai 201508China

To the Editor:Coronavirus disease 2019(COVID-19),instigated by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),has manifested as a formidable global pandemic,precipitating significant economic implications and leading to extensive mortalities.Simultaneously,the acquired immune deficiency syndrome(AIDS)epidemic caused by the human immunodeficiency virus(HIV)persists as a salient global health exigency.Cumulatively by the termination of 2022,AIDS-related pathologies accounted for approximately 40.4 million fatalities,with an extant global HIV population of roughly 39 million people living with HIV(PLWH),as a potentially immunocompromised population,have received limited attention in the realm of COVID-19 research.

关键词： acute termination living

Artificial intelligence-assisted niacin skin flush screening in early psychosis identification and prediction

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General Psychiatry 2022年第2期35卷 76-78页

作者： Tao Chen Haichun Liu renfang Tian Ranpiao Gan Wenzuo Xu Tianhong zhang Jijun Wang Department of Economics University of WaterlooWaterlooOntarioCanada Depatment of Statistics and Actuarial Science(Cross Appointed) University of Waterloo WaterlooOntarioCanada Big Data Research Lab Universty of WaterlooWaterlooOntarioCanada Shanghai Niacin Tech Co. LtdShanghal China Labor and Worklife Program Harvard UniversityCambridgeMassachusettsUSA Department of Automation Shanghai Jao Tong UniversityShanghaiChina Ningbo Artificial Intelligent Institute Shanghai Jiao Tong UniversityNingboChina School of Management Economicsand MathematicsKing's University CollegeLondon0ntarioCanada Shanghai Intelligent Psychological Evaluaton and Intervention Engineering Technology Research Center Shanghai Key Laboratory of Psychotic DisordersShanghai Mental Health CenterShanghai Jiao Tong University School of MedicineShanghaiChina Bio-X Institutes Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders(Ministry of Education)ShanghaiChina CAS Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of SciencesShanghaiChina Institute of Psychology and Behavioral Science Shanghai Jiao Tong UniversiyShanghaiChina

Schizophrenia is a devastating mental disorder affecting 20 million people worldwide.Early diagnosis is crucial for disease management and improvement in prognosis,and diagnostic biomarkerscan serveasobjective indicators for the early screening of the disease.Based on the observation of diminished flush responses to niacin in patients with schizophrenia Horrobin proposed anoninvasive niacin skin flush screening for schizophrenia.

关键词： diagnosis prognosis prediction

Pectin Methylesterases Enhance Root Cell Wall Phosphorus Remobilization in Rice

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Rice science 2022年第2期29卷 179-188页

作者： WU Qi TAO Ye zhang Xiaolong DONG Xiaoying XIA Jixing SHEN renfang ZHU Xiaofang State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources College of Life Science and TechnologyGuangxi UniversityNanning 530004China

Pectin contributes greatly to cell wall phosphorus(P)remobilization.However,it is currently unclear whether the methylesterification degree of the pectin,which is related to the activity of pectin methylesterases(PMEs),is also involved in this process.Here,we demonstrated that elevated PME activity can facilitate the remobilization of P deposited in the cell wall.P-deficient conditions resulted in the reduction of root cell wall P content.This reduction was more pronounced in Nipponbare than in Kasalath,in company with a significant increment of the PME activity,indicating a possible relationship between elevated PME activity and cell wall P remobilization.This hypothesis was supported by in vitro experiments,as pectin with lower methylesterification degree had higher ability to release inorganic P(Pi)from insoluble FePO_(4).Furthermore,among the 35 OsPME members in rice,only the expression of OsPME14 showed a relationship with PME activity.In addition,transgenic rice lines overexpressing OsPME14 had increased PME activity,released more P from the root cell wall,and more resistant to P deficiency.In conclusion,PMEs enhance P remobilization in P-starved rice by increasing PME activity in Nipponbare,which in turn helps to remobilize P from the cell wall,and thus makes more available P.

关键词： cell wall phosphorus pectin methylesterase remobilization rice

Super-enhancer receives signals from the extracellular matrix to induce PD-L1-mediated immune evasion via integrin/ BRAF/TAK1/ERK/ETV4 signaling

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Cancer Biology & Medicine 2022年第5期19卷 669-684页

作者： Panpan Ma Xinxin Jin Zhiwei Fan Zhou Wang Suhui Yue Changyue Wu Shiyin Chen Yuanyuan Wu Miaomiao Chen Donghua Gu Siliang zhang renfang Mao Yihui Fan Laboratory of Medical Science School of MedicineNantong UniversityNantong 226001China Department of Pathogenic Biology School of MedicineNantong UniversityNantong 226001China Department of Clinical Laboratory Yancheng No.1 People’s HospitalYancheng 224005China School of Life Sciences Nantong UniversityNantong 226001China Department of Dermatology Affiliated Hospital of Nantong UniversityNantong UniversityNantong 226001China The Department of Urology the Second Affiliated Hospital of Nantong UniversityNantong UniversityNantong 226001China The Department of Radiotherapy Oncology Harbin Medical University Cancer HospitalHarbin 150086China Department of Pathophysiology School of MedicineNantong UniversityNantong 226001China

Objective:PD-L1 and PD-L2 expression levels determine immune evasion and the therapeutic efficacy of immune checkpoint blockade.The factors that drive inducible PD-L1 expression have been extensively studied,but mechanisms that result in constitutive PD-L1 expression in cancer cells are largely unknown.Methods:DNA elements were deleted in cells by CRISPR/Cas9-mediated knockout.Protein function was inhibited by chemical inhibitors.Protein levels were examined by Western blot,mRNA levels were examined by real-time RT-PCR,and surface protein expression was determined by cellular immunofluorescence and flow cytometry.Immune evasion was examined by in vitro T cell-mediated killing.Results:We determined the core regions(chr9:5,496,378–5,499,663)of a previously identified PD-L1L2-super-enhancer(SE).Through systematic analysis,we found that the E26 transformation-specific(ETS)variant transcription factor(ETV4)bound to this core DNA region but not to DNA surrounding PD-L1L2SE.Genetic knockout of ETV4 dramatically reduced the expressions of both PD-L1 and PD-L2.ETV4 transcription was dependent on ERK activation,and BRAF/TAK1-induced ERK activation was dependent on extracellular signaling fromαvβ3 integrin,which profoundly affected ETV4 transcription and PD-L1/L2 expression.Genetic silencing or pharmacological inhibition of components of the PD-L1L2-SE-associated pathway rendered cancer cells susceptible to T cell-mediated killing.Conclusions:We identified a pathway originating from the extracellular matrix that signaled via integrin/BRAF/TAK1/ERK/ETV4 to PD-L1L2-SE to induce PD-L1-mediated immune evasion.These results provided new insights into PD-L1L2-SE activation and pathways associated with immune checkpoint regulation in cancer.

关键词： PD-L1 super-enhancer ETV4 ERK αvβ3 integrin BRAF TAK1

比克恩丙诺片与拉米夫定多替拉韦片对初治艾滋病期患者的疗效及安全性分析

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中华传染病杂志 2024年第3期42卷 147-153页

作者：王江蓉孙建军杨君洋张仁芳刘莉陈军汤阳齐唐凯宋炜王珍燕沈银忠上海市(复旦大学附属)公共卫生临床中心感染与免疫科上海201508

目的评价并比较含整合酶抑制剂的单片制剂方案比克恩丙诺片(B/F/TAF)与拉米夫定多替拉韦片(3TC/DTG)用于初治艾滋病期患者的临床疗效及安全性。方法本研究为一项回顾性队列研究,通过收集2020年10月至2023年7月在上海市(复旦大学附属)公... 详细信息

目的评价并比较含整合酶抑制剂的单片制剂方案比克恩丙诺片(B/F/TAF)与拉米夫定多替拉韦片(3TC/DTG)用于初治艾滋病期患者的临床疗效及安全性。方法本研究为一项回顾性队列研究,通过收集2020年10月至2023年7月在上海市(复旦大学附属)公共卫生临床中心使用B/F/TAF或3TC/DTG作为初始抗反转录病毒治疗方案且持续用药时间≥24周的艾滋病期患者的临床资料,比较B/F/TAF组与3TC/DTG组基线人类免疫缺陷病毒(HIV)-1 RNA,基线和治疗12周的CD4+T淋巴细胞计数,治疗24周的病毒学抑制率和病毒学失败率,以及治疗前后总胆固醇、血肌酐、尿酸等指标变化。统计学分析采用独立样本t检验、校正t检验、曼-惠特尼U检验、威尔科克森符号秩检验、χ^(2)检验。结果189例初治艾滋病期患者中,B/F/TAF组141例,3TC/DTG组48例。B/F/TAF组患者基线HIV-1 RNA为1.77(0.78,4.52)×10^(5)拷贝/mL,3TC/DTG组为0.97(0.24,2.20)×10^(5)拷贝/mL,差异有统计学意义(U=2221.00,P=0.006)。B/F/TAF组治疗24周病毒学抑制率为77.3%(109/141),无病毒学失败的患者;3TC/DTG组治疗24周病毒学抑制率为85.4%(41/48),1例(2.1%)病毒学失败。治疗12周时,B/F/TAF组CD4+T淋巴细胞计数较基线升高30%以上的患者占92.2%(130/141),3TC/DTG组为85.4%(41/48);B/F/TAF组CD4+T淋巴细胞计数较基线升高>100.00/μL的比例为67.4%(95/141),3TC/DTG组为52.1%(25/48);两组间差异均无统计学意义(χ^(2)=1.91、3.61,P=0.167、0.733)。B/F/TAF组和3TC/DTG组患者治疗24周时总胆固醇(W=2036.00,t=-5.42)、血肌酐(W=1098.00、234.00)、尿酸(W=2188.00、299.00)水平均较治疗前升高,轻中度肾功能不全患者比例(χ^(2)=22.29、8.22)均较治疗前增加,差异均有统计学意义(均P<0.01)。结论B/F/TAF与3TC/DTG用于初治艾滋病期患者具有良好的病毒学、免疫学疗效和安全性。

关键词：获得性免疫缺陷综合征安全性有效性比克恩丙诺片拉米夫定多替拉韦片

农田土壤重金属污染现状与安全利用技术研究进展

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土壤 2024年第2期56卷 229-241页

作者：余琼阳李婉怡张宁赵玲王月梅宋家音马文亭张光华董荷玲於维维滕应骆永明沈仁芳台州市农业技术推广中心浙江台州318000 台州市路桥区农(渔)技总站浙江台州318000 中国科学院南京土壤研究所南京210008

重金属污染农田直接影响粮食安全和农业可持续发展。安全利用技术主要通过降低土壤中重金属的生物有效性,以减少作物对重金属的吸收累积和消减重金属进入食物链的风险,其对解决我国重金属污染农田有效利用和保障粮食安全生产具有重要意... 详细信息

重金属污染农田直接影响粮食安全和农业可持续发展。安全利用技术主要通过降低土壤中重金属的生物有效性,以减少作物对重金属的吸收累积和消减重金属进入食物链的风险,其对解决我国重金属污染农田有效利用和保障粮食安全生产具有重要意义。因此,本文首先分析了农田土壤重金属污染成因与现状,然后综述了不同安全利用技术,如农艺调控措施(包括低累积作物品种筛选、水肥管理及间作或轮作耕作方式)、原位钝化改良和叶面阻控技术的研究进展,最后提出了强化重金属污染农田土壤安全利用研究及应用的对策。

关键词：重金属污染现状安全利用农田土壤