检索结果-南通市图书馆

ca^(2+) binding to the c_(2)E domain of otoferlin is required for hair cell exocytosis and hearing

Protein & cell 2024年第4期15卷 305-312页

作者： Han chen Mehar Monga Qinghua Fang Loujin Slitin Jakob Neef Shashank S.chepurwar regina c.lia mingroni netto Karina Lezirovitz Alfredo Tabith,Jr. Fritz Benseler Nils Brose Kathrin Kusch carolin Wichmann Nicola Strenzke Barbara Vona Julia Preobraschenski Tobias Moser Institute for Auditory Neuroscience and InnerEarLab University Medical Center Göttingen37075 GöttingenGermany collaborative Research center 889 University of Göttingen37075 GöttingenGermany Auditory Neuroscience and Synaptic Nanophysiology Group Max Planck Institute for Multidisciplinary Sciences37075 GöttingenGermany Göttingen Graduate center for Neurosciences Biophysics and Molecular BiosciencesUniversity of Göttingen37075 GöttingenGermany Biochemistry of Membrane Dynamics Group Institute for Auditory NeuroscienceUniversity Medical Center Göttingen37075 GöttingenGermany Molecular Architecture of Synapses Group Institute for Auditory Neuroscience and InnerEarLabUniversity Medical Center Göttingen37075 GöttingenGermany center for Biostructural Imaging of Neurodegeneration University Medical Center Göttingen37075 GöttingenGermany Auditory Systems Physiology Group Institute for Auditory Neuroscience and InnerEarLabUniversity Medical Center Göttingen37075 GöttingenGermany Departamento de Genética e Biologia Evolutiva Centro de Pesquisas sobre o Genoma Humano e Células-TroncoInstituto de BiociênciasUniversidade de São PauloSão Paulo 05508-220Brazil Laboratório de Otorrinolaringologia/LIM32 Faculdade de MedicinaHospital das ClínicasUniversidade de São PauloSão PauloSP 05508-220Brazil DERDIc Pontifícia Universidade Católica de São PauloSão Paulo 05508-220Brazil Department of Molecular Neurobiology Max Planck Institute for Multidisciplinary Sciences37075 GöttingenGermany Multiscale Bioimaging cluster of Excellence(MBExc) University of Göttingen37075 GöttingenGermany Functional Auditory Genomics Group Auditory Neuroscience and Optogenetics LaboratoryGerman Primate Center37077 GöttingenGermany Hearing Genomics Group Institute for Auditory Neuroscience and InnerEarLabUniversity Medical Center Göttingen37075 GöttingenGermany Institute of Human Genetics University Medical Center Göttingen37075 GöttingenGermany

Dear Editor,Afferent synapses of cochlear inner hair cells(IHcs)employ a unique molecular machinery(see extended background in Supplementary Materials).Otoferlin is a key player in this machinery and its defects cause human auditory synaptopathy(Moser and Starr,2016).Otoferlin,a tail-anchored(Vogl et al.,2016)multi-c_(2)-domain protein(Fig.1Ai)specific to hair cells(Roux et al.,2006),is a member of the ferlin protein family involved in membrane trafficking and repair that are of major disease relevance(Pangršičet al.,2012),also see Supplementary *** is distributed broadly within IHcs(Fig.2Ai-Aiii;Pangrsic et al.,2010;Roux et al.,2006).

关键词： auditory hair

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Anticancer therapeutic strategies for targeting mutant p53-Y220c

引用

Journal of Biomedical Research 2024年第3期38卷 222-232页

作者： Vitaly chasov Damir Davletshin Elvina Gilyazova regina Mirgayazova Anna Kudriaeva Raniya Khadiullina Youyong Yuan Emil Bulatov Institute of Fundamental Medicine and Biology Kazan Federal UniversityKazan 420008Russia Shemyakin-Ovchinnikov Institute of Bioorganic chemistry Russian Academy of SciencesMoscow 117997Russia Institute of Life Sciences School of MedicineSouth China University of TechnologyGuangzhouGuangdong 510006China

The tumor suppressor p53 is a transcription factor with a powerful antitumor activity that is controlled by its negative regulator murine double minute 2(MDM2,also termed HDM2 in humans)through a feedback *** the same time,TP53 is the most frequently mutated gene in human *** p53 proteins lose wild-type p53 tumor suppression functions but acquire new oncogenic properties,among which are deregulating cell proliferation,increasing chemoresistance,disrupting tissue architecture,and promoting migration,invasion and metastasis as well as several other pro-oncogenic *** oncogenic p53 mutation Y220c creates an extended surface crevice in the DNA-binding domain destabilizing p53 and causing its denaturation and *** cavity accommodates stabilizing small molecules that have therapeutic *** development of suitable small-molecule stabilizers is one of the therapeutic strategies for reactivating the Y220c mutant *** this review,we summarize approaches that target p53-Y220c,including reactivating this mutation with small molecules that bind Y220c to the hydrophobic pocket and developing immunotherapies as the goal for the near future,which target tumor cells that express the p53-Y220c neoantigen.

关键词： p53 Y220c mutation small molecule DNA-binding domain immunotherapy T cell receptor mimic antibody

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

clinicopathological Description of a chronic Lymphocytic Leukemia in a Dog

引用

Open Journal of Veterinary Medicine 2024年第7期14卷 155-162页

作者： Maria clara Boni Raffi Giovanna Valverde Magalhães Barbosa Alana carolina capais Rodrigues Sheila canevese Rahal John P. Kastelic Luíz Guilherme Dercore Benevenuto Iolanda Simões Braga Beatriz Poloni Batista Felipe césar Da Silva Brasileiro Fernanda Barthelson carvalho de Moura Priscylla Tatiana chalfun Guimarães Okamoto regina Kiomi Takahira Alessandra Melchert Noeme Sousa Rocha Department of Veterinary clinics School of Veterinary Medicine and Animal Science So Paulo State UniversityUNESP Botucatu SP Brazil Independent Veterinary Practitioner Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science So Paulo State UniversityUNESP Botucatu SP Brazil Faculty of Veterinary Medicine University of Calgary Calgary Canada

Hematopoietic tumors are associated with diagnostic and therapeutic challenges and a poor prognosis. Furthermore, their pathophysiology in dogs is not well understood. chronic lymphocytic leukemia has been rarely reported in golden retrievers, perhaps due to difficulties in diagnosis and care. An 8-year-old spayed female golden retriever was referred to our hospital with a 2-month history of fatigue, weakness, and selective appetite. The dog was diagnosed with chronic lymphocytic leukemia based on blood smear evaluation. Despite treatment, the dog deteriorated and the owners opted for euthanasia. Histopathological examination revealed atypical lymphocytes in the spleen, extramedullary hematopoiesis in the spleen and liver, bone marrow depletion, moderate neutrophilic myocarditis, and acute renal tubular necrosis. In an immunohistochemical panel, cells in the spleen and bone marrow were positive for anti-cD20, anti-cD45, anti-cD3, anti-IBA-1, anti-Ki67, anti-MUM-1, and anti-PAX-5. In conclusion, the leukemia diagnosis was confirmed through reactive histiocytosis, marked extramedullary hematopoiesis, and other clinical and laboratory findings. Herein, we report a case of chronic lymphocytic leukemia in a female dog with clinical, laboratory, and necroscopy findings.

关键词： canine cancer Neoplasia Therapeutics

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Importance of complementary data to histopathological image analysis of oral leukoplakia and carcinoma using deep neural networks

引用

Intelligent Medicine 2023年第4期3卷 258-266页

作者： Leandro Muniz de Lima Maria clara Falcão Ribeiro de Assis Júlia Pessini Soares Tânia regina Grão-Velloso Liliana Aparecida Pimenta de Barros Danielle Resende camisasca Renato Antonio Krohling Nature-inspired computing Lab Federal University of Espirito SantoVitoriaBrazil Graduate Program in computer Science Federal University of Espirito SantoVitoriaBrazil School of Dentistry Departamento de Clinica OdontologicaFederal University of Espirito SantoVitoriaBrazil Graduate Program in clinical Dentistry Federal University of Espirito SantoVitoriaBrazil

Background Oral cancer is one of the most common types of cancer in men causing mortality if not *** recent years,computer-aided diagnosis(cAD)using artificial intelligence techniques,in particular,deepneural networks have been investigated and several approaches have been proposed to deal with the automateddetection of various pathologies using digital *** studies indicate that the fusion of images with thepatient’s clinical information is important for the final clinical *** such dataset does not yet exist fororal cancer,as far as the authors are aware,a new dataset was collected consisting of histopathological images,demographic and clinical *** study evaluated the importance of complementary data to histopathologicalimage analysis of oral leukoplakia and carcinoma for *** A new dataset(NDB-UFES)was collected from 2011 to 2021 consisting of histopathological imagesand *** 237 samples were curated and analyzed by oral pathologists generating the gold standardfor ***-of-the-art image fusion architectures and complementary data(concatenation,MutualAttention,MetaBlock and MetaNet)using the latest deep learning backbones were investigated for 4 distincttasks to identify oral squamous cell carcinoma,leukoplakia with dysplasia and leukoplakia without *** evaluate them using balanced accuracy,precision,recall and area under the ROc curve *** Experimental results indicate that the best models present balanced accuracy of 83.24%using images,demographic and clinical information with MetaBlock fusion and ResNetV2 *** represents an improvement in performance of 30.68%(19.54 pp)in the task to differentiate samples diagnosed with oral squamous cellcarcinoma and leukoplakia with or without *** This study indicates that cured demographic and clinical data may positively influence the performance of artificial intelligence models in automated classification of

关键词： Leukoplakia Squamous carcinoma Pathology Deep learning Transfer learning

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Extract of codiaeum luzonicum Merr. overcomes multidrug resistance in human colon cancer cells by modulating P-glycoprotein

引用

Asian Pacific Journal of Tropical Biomedicine 2022年第9期12卷 400-410页

作者： regina Joyce E.Ferrer Marc Justin c.Ong Sonia D.Jacinto Institute of Biology University of the Philippines DilimanQuezon CityMetro ManilaPhilippines

Objective:To investigate anti-multidrug resistance(MDR)activity and safety of the bioactive fraction(cL11)from codiaeum luzonicum crude leaf ***:cytotoxic activity of cL11 against MDR and nonresistant colon cancer cells was assessed using MTT *** of cell death was investigated by annexin V-propidium iodide staining,TUNEL,and Jc-1 *** examine mechanism of action,the effect on the expression and function of the MDR-implicated protein P-glycoprotein was tested using Western blotting and calcein assay,***:cL11 had an Ec50 of 0.18,1.03 and 38.52µg/mL against HcT-15,HcT-15/Dox and HcT116,*** was mediated by inhibition of P-glycoprotein function and *** mode of cell death involved mitochondrial membrane depolarization and was mostly non-apoptotic at Ec50 concentrations against HcT-15 and HcT-15/***:Fraction cL11 of codiaeum luzonicum induces nonapoptotic cell death in MDR cancer cells by overcoming MDR through inhibition of P-glycoprotein expression and function.

关键词： Natural product Leaf extract codiaeum luzonicum MDR cancer P-gp inhibitor colorectal cancer

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bacille-calmette-Guerin modulates human macrophage and dendritic cell response to SARS-coV-2 S-glycoprotein

Infectious Medicine

引用

Infectious Medicine 2023年第3期2卷 241-245页

作者： regina c.Ambe Shubhang Bhalla Alejandra Alvarado Jose Barragan Jorge cervantes Paul L.Foster School of Medicine Texas Tech University Health Sciences CenterEl PasoTX 79905USA Dr Kiran c.Patel college of Allopathic Medicine Nova Southeastern UniversityFort LauderdaleFL 33328USA

Background:Given that epidemiological evidence suggests a potential protective role for Bacille-calmette-Guerin against cOVID-19,we aimed to explore whether pre-exposure of human monocyte-derived macrophages and dendritic cells to BcG could modulate their response to SARS-coV-2 ***:Dual THP-1 cells containing 2 reporter plasmids for transcription factors NF-κB,and IRF were differ-entiated into macrophages over 3 days using phorbol 12-myristate 13-acetate,or into dendritic cells over 6 days using commercial monocyte-dencritic cell differentiation media and matured with recombinant tumor necrosis factor-α.cells were exposed to BcG for 24 h and then stimulated with SARS-coV-2 S-glycoprotein for 24 ***:Pre-exposure of human macrophages and Dcs to BcG increased IRF and NF-kb activation in response to the SARS-coV-2 ***:Our results showed that pre-exposure of both types of cells to BcG exhibited an increase in inflam-matory transcription factors upon stimulation with ***-induced trained immunity may be an important tool for reducing susceptibility to SARS-coV-2 infection and severity of *** findings help in the design of future BcG-based therapeutic approaches in the treatment of diseases caused by viral infections.

关键词： BcG SARS-coV-2 Dendritic cell Macrophage

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

cajal Bodies Are Developmentally Regulated during Pollen Development and Pollen Tube Growth in Arabidopsis thaliana

引用

Molecular Plant 2013年第4期6卷 1355-1357页

作者： regina Scarpin Lorena Sigaut lia Pietrasanta Sheila Mccormick Binglian Zheng Jorge Muschietti Instituto de Ingeniería Genética y Biología Molecular (INGEBI-cONIcET) Vuelta de Obligado 2490 Piso 2 Buenos Aires 1428 Argentina centro de Microscopías Avanzadas Universidad de Buenos Aires 1428 Buenos Aires Argentina Plant Gene Expression center US Department of Agriculture/Agricultural Research Service and Department of Plant and Microbial Biology University of California at Berkeley Albany CA 94710 USA State Key Laboratory of Genetic Engineering and Institute of Plant Biology School of Life Sciences Fudan University Shanghai 200433 China Departamento de Biodiversidad y Biología Experimental Facultad de Ciencias Exactas y Naturales Universidad de Buenos AiresBuenos Aires 1428 Argentina

Dear Editor, cajal bodies （cBs） are sub-nuclear bodies first described in neurons by Ramon y cajal in 1903 and subsequently character- ized in both plant and animals （Gall, 2003）. They appear close to the nucleoli as discrete foci whose size and number vary during the cell cycle （Strzelecka et al., 2010）. They are dynamic structures that move, fuse, and separate, depending on the transcriptional status of the cell （cioce and Lamond, 2005）. cBs are involved in assembly and trafficking of small nucleolar ribonucleopro- teins （snoRNPs） and in assembly and trafficking of spliceoso- mal small nuclear ribonucleoprotein （snRNPs） complexes that are involved in mRNA splicing （Gall, 2003; cioce and Lamond, 2005）. cBs also contain components of the machinery involved in siRNA-mediated silencing and in methylation of repetitive DNA （Li et al., 2006）.

关键词： Developmentally Regulated Arabidopsis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Vitamin D deficiency,functional status,and balance in older adults with osteoarthritis

引用

World Journal of clinical cases 2021年第31期9卷 9491-9499页

作者： cláudia N Montemor Marcos Tadeu P Fernandes Audrey S Marquez regina c.lia Poli-Frederico Rubens Alexandre da Silva Karen B P Fernandes Laboratory of Rehabilitation Research Doctoral Program of Rehabilitation SciencesUniversity Pitágoras Unopar(UNOPAR)Londrina 86041-140ParanaBrazil Department of Anesthesiology Irmandade da Santa Casa de LondrinaLondrina 86010-160ParanaBrazil Health Sciences Research center University Pitágoras Unopar(UNOPAR)Londrina 86041-140ParanaBrazil Doctoral Program of Rehabilitation Sciences University Pitagoras Unopar(UNOPAR)Londrina 86041-140ParanaBrazil Département des Sciences de la Santé Centre intersectoriel en santédurableLaboratoire de recherche BioNRUniversitédu QuébecàChicoutimi(UQAC)SaguenayG7H 2B1QuébecCanada centre intégréde santéet services sociaux du Saguenay-Lac-Saint-Jean(cIUSSS SLSJ) Hôpital de La Baie-Services gériatriques spécialisésSaguenayQuébecG7H 7K9Canada

BAcKGROUND Low vitamin D levels are associated with a more severe case of knee osteoarthritis(OA).However,there are few published reports concerning an association between vitamin D deficiency and functional status of individuals with OA and no reports about postural balance in this *** To analyze the relationship between vitamin D deficiency and severity,functional status,and balance in elderly patients with *** In this cross-sectional study,105 elderly patients with hip and knee OA were *** severity was assessed by the Kellgren-Lawrence *** functional status was assessed with the Lequesne *** balance was assessed using a force platform,and center-of-pressure parameters(velocity at anteroposterior and mediolateral axis)were used as the balance *** 25(OH)vitamin D levels were measured using a chemiluminescence *** Most of the patients(mean age:70.6±6.5 years)were female(n=78,74.3%).In the group with vitamin D deficiency,43 patients(56.6%)had severe OA,while 33 patients(43.4%)had mild or moderate OA(χ^(2) test,P=0.04).Patients with vitamin D deficiency showed a higher Lequesne index score(Mann-Whitney test,P=0.04),indicating a worse functional impairment when compared to individuals with normal vitamin D ***,patients with vitamin D deficiency had worse postural balance according to the Mann-Whitney test(P=0.03).cONcLUSION Vitamin D deficiency is associated with worse severity,functional status,and postural balance in patients with OA.

关键词： Osteoarthritis Elderly Vitamin D Disability Severity Balance

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The role of zooplankton as live feeds on the thyroid hormone profile related to metamorphosis of marine fish larvae coral trout Plectropomus leopardus (Lacepède, 1802)

引用

Aquaculture and Fisheries 2022年第2期7卷 179-184页

作者： regina Melianawati Rarastoeti Pratiwi Nyoman Puniawati Pudji Astuti Institute for Mariculture Research and Fisheries Extension Jl.Singaraja-GilimanukBulelengBali81119Indonesia Faculty of Biology Universitas Gadjah MadaJl.Teknika Selatan Sekip UtaraYogyakarta55164Indonesia Faculty of Veterinary Medicine Universitas Gadjah MadaJl.Fauna 2KarangmalangYogyakarta55164Indonesia

coral trout Plectropomus leopardus is a high-economical value of marine fish ***,the larviculture for this species should be performed in hatchery scale for their *** of the important thing in larval stage is hormonal *** hormone is known plays an important role in the metamorphosis of marine fish *** source of that hormone is assumed derived from zooplankton as their live *** live feeds commonly used for marine fish larvae in hatchery are zooplankton copepods and *** purpose of this research to determine the influence of feeding copepods and rotifers on thyroid hormone profile activity,consisted of triiodothyronine(T3)and thyroxine(T4),related to the metamorphoses of coral trout *** research was conducted using three feeding schemes:copepods(A),copepods-rotifers(B),and rotifers(c).It was done for 50 Days After Hatching(DAH).The results showed that larvae from treatment(A)and(B)achieved the highest level of T3 and T4 earlier at 30 DAH,which indicated that both of those larvae have entered the metamorphosis stage *** the other hand,larvae from treatment(c)achieved it and entered the metamorphosis stage later at 40 *** addition,iodine levels in the copepods were significantly higher than those of in rotifers(Pch,live feeds copepods and rotifers have effect on thyroid hormone *** have better potential in accelerating the metamorphosis achievement of coral trout larvae with the fact that they have higher iodine content.

关键词： coral trout larvae copepods Rotifers Feeding effect Thyroid hormone Metamorphosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Propranolol hydrochloride degradation using La@TiO_(2) functionalized with cMcD

引用

Journal of Rare Earths 2022年第4期40卷 579-585,I0003页

作者： Gustavo Lopes colpani Vitor Follmann Santos Rubieli carla Frezza Zeferino Micheli Zanetti Josiane Maria Muneron de Mello Luciano Luiz Silva Natan Padoin regina de Fatima Peralta Muniz Moreira Márcio Antônio Fiori cíntia Soaresc Post-Graduate Program in Technology and Innovation Management Community University of ChapecóRegionChapecó89809-900Brazil Department of chemical Engineering Community University of ChapecóRegionChapecó89809-900Brazil Laboratory of Materials and Scientific computing(LabMAc) Department of Chemical and Food EngineeringFederal University of Santa CatarinaFlorianópolis88040-900Brazil

Propranolol hydrochloride can be considered a persistent and bioaccumulative pharmaceutical in the *** drug and its by-products are potentially toxic and have adverse effects,since these compounds have been associated with endocrine-disrupting effects,reproductive deficiencies,embryo abnormalities and pericardial ***_(2)–La 0.05%–carboxymethyl-β-cyclodextrin(cMcD)nanoparticles were successfully prepared by a simple two-step method,which consists of sonification and *** characterization analyses reveal that lanthanum is dispersed on the semiconductor surface,probably forming Ti–O–La bonds,which can induce oxygen vacancies and surface defects that effectively restrain the recombination of photogenerated electron/holes *** efficiency of TiO_(2)–La 0.05%–cMcD samples in degradation of propranolol under UV-light irradiation is higher than that of pristine TiO_(2) within 20 min reaction,probably due to complex formation between theβ-blocker and the oligosaccharide,which allows us to propose a photocatalytic mechanism based on the formation of intermediates and competition of these compounds to the radicals and cMcD cavities.

关键词： TiO_(2) Lanthanum cMcD Propranolol Photocatalysis Functionalization

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：