Bacille-Calmette-Guerin modulates human macrophage and dendritic cell response to SARS-CoV-2 S-glycoprotein
作者机构：Paul L.Foster School of MedicineTexas Tech University Health Sciences CenterEl PasoTX 79905USA Dr Kiran C.Patel College of Allopathic MedicineNova Southeastern UniversityFort LauderdaleFL 33328USA
出 版 物：《Infectious Medicine》 (感染医学（英文）)
年 卷 期：2023年第2卷第3期
页 面：241-245页
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
主 题：BCG SARS-CoV-2 Dendritic cell Macrophage
摘 要：Background:Given that epidemiological evidence suggests a potential protective role for Bacille-Calmette-Guerin against COVID-19,we aimed to explore whether pre-exposure of human monocyte-derived macrophages and dendritic cells to BCG could modulate their response to SARS-CoV-2 ***:Dual THP-1 cells containing 2 reporter plasmids for transcription factors NF-κB,and IRF were differ-entiated into macrophages over 3 days using phorbol 12-myristate 13-acetate,or into dendritic cells over 6 days using commercial monocyte-dencritic cell differentiation media and matured with recombinant tumor necrosis factor-α.Cells were exposed to BCG for 24 h and then stimulated with SARS-CoV-2 S-glycoprotein for 24 ***:Pre-exposure of human macrophages and DCs to BCG increased IRF and NF-kb activation in response to the SARS-CoV-2 ***:Our results showed that pre-exposure of both types of cells to BCG exhibited an increase in inflam-matory transcription factors upon stimulation with ***-induced trained immunity may be an important tool for reducing susceptibility to SARS-CoV-2 infection and severity of *** findings help in the design of future BCG-based therapeutic approaches in the treatment of diseases caused by viral infections.
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