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Molecular markers for artemisinin and partner drug resistance in natural Plasmodium falciparum populations following increased insecticide treated net coverage along the slope of mount Cameroon:cross-sectional study

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Infectious Diseases of Poverty 2017年第1期6卷 1205-1214页

作者： Tobias O.Apinjoh regina n.mugri Olivo Miotto Hanesh F.Chi Rolland B.Tata Judith K.Anchang-Kimbi Eleanor M.Fon Delphine A.Tangoh Robert V.nyingchu Christopher Jacob Roberto Amato Abdoulaye Djimde Dominic Kwiatkowski Eric A.Achidi Alfred Amambua-ngwa Department of Biochemistry and Molecular Biology University of BueaBueaCameroon Department of Microbiology and Parasitology University of BueaBueaCameroon Mahidol-Oxford Research Unit Faculty of Tropical MedicineMahidol UniversityBangkokThailand Department of Zoology and Animal Physiology University of BueaBueaCameroon Department of Medical Laboratory Science University of BueaBueaCameroon Wellcome Trust Sanger Institute HinxtonCambridge CB101SAUK Malaria Research and Training Centre BamakoMali MRC Unit FajaraThe Gambia

Background:Drug resistance is one of the greatest challenges of malaria control programmes,with the monitoring of parasite resistance to artemisinins or to Artemisinin Combination Therapy(ACT)partner drugs critical to elimination *** of resistance to a wide panel of antimalarials were assessed in natural parasite populations from southwestern ***:Individuals with asymptomatic parasitaemia or uncomplicated malaria were enrolled through crosssectional surveys from May 2013 to March 2014 along the slope of mount *** falciparum malaria parasitaemic blood,screened by light microscopy,was depleted of leucocytes using CF11 cellulose columns and the parasite genotype ascertained by sequencing on the Illumina HiSeq ***:A total of 259 participants were enrolled in this study from three different *** some alleles associated with drug resistance in pfdhfr,pfmdr1 and pfcrt were highly prevalent,less than 3%of all samples carried mutations in the pfkelch13 gene,none of which were amongst those associated with slow artemisinin parasite clearance rates in Southeast *** most prevalent haplotypes were triple mutants PfdhfrI51R59n108I164(99%),pfcrt-C72V73I74E75T76(47.3%),and single mutants PfdhpsS_(436)G_(437)K_(540)A_(581)A_(613)(69%)and Pfmdr1 n_(86)F)(184)D_(1246)(53.2%).Conclusions:The predominance of the Pf pfcrt CVIET and Pf dhfr IRn triple mutant parasites and absence of pfkelch13 resistance alleles suggest that the amodiaquine and pyrimethamine components of AS-AQ and SP may no longer be effective in their role while chloroquine resistance still persists in southwestern Cameroon.

关键词： Molecular markers Drug resistance Plasmodium falciparum

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Liver Characterization of a Cohort of Alpha-1 Antitrypsin Deficiency Patients with and without Lung Disease

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Journal of Clinical and Translational Hepatology 2024年第10期12卷 845-856页

作者： naweed Mohammad regina Oshins Tongjun Gu Virginia Clark Jorge Lascano naziheh Assarzadegan George Marek Mark Brantly nazli Khodayari Division of Pulmonary Critical Care and Sleep MedicineCollege of MedicineUniversity of FloridaGainesvilleFLUSA Interdisciplinary Center for Biotechnology Research Bioinformatics CoreUniversity of FloridaGainesvilleFLUSA Division of Gastroenterology Hepatology and NutritionCollege of MedicineUniversity of FloridaGainesvilleFLUSA Division of Pathology Immunologyand Laboratory MedicineCollege of MedicineUniversity of FloridaGainesvilleFLUSA Division of Gastroenterology and Hepatology Mayo ClinicRochesterMNUSA

Background and Aims:Alpha-1 antitrypsin deficiency(AATD)is a genetic disorder characterized by the misfolding and accumulation of the mutant variant of alpha-1 antitrypsin(AAT)within hepatocytes,which limits its access to the circulation and exposes the lungs to protease-mediated tissue *** results in progressive liver disease secondary to AAT polymerization and accumulation,and chronic obstructive pulmonary disease(COPD)due to deficient levels of AAT within the *** goal was to characterize the unique effects of COPD secondary to AATD on liver disease and gene ***:A subcohort of AATD individuals with COPD(n=33)and AATD individuals without COPD(n=14)were evaluated in this study from our previously reported cross-sectional *** used immunohistochemistry to assess the AATD liver phenotype,and RnA sequencing to explore liver *** observed a distinct transcriptomic profile in liver tissues from AATD individuals with COPD compared to those ***:A total of 339 genes were differentially *** pathways related to fibrosis,extracellular matrix remodeling,collagen deposition,hepatocellular damage,and inflammation were significantly upregulated in the livers of AATD individuals with *** analysis also revealed higher levels of fibrosis and hepatocellular damage in these ***:Our data supports a relationship between the development of COPD and liver disease in AATD and introduces genes and pathways that may play a role in AATD liver disease when COPD is *** believe addressing lung impairment and airway inflammation may be an approach to managing AATD-related liver disease.

关键词： Alpha-1 antitrypsin deficiency Chronic obstructive pulmonary disease Liver fibrosis Liver biopsy Liver histology Transcriptomics

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Ca^(2+) binding to the C_(2)E domain of otoferlin is required for hair cell exocytosis and hearing

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Protein & Cell 2024年第4期15卷 305-312页

作者： Han Chen Mehar Monga Qinghua Fang Loujin Slitin Jakob neef Shashank S.Chepurwar regina Célia Mingroni netto Karina Lezirovitz Alfredo Tabith,Jr. Fritz Benseler nils Brose Kathrin Kusch Carolin Wichmann nicola Strenzke Barbara Vona Julia Preobraschenski Tobias Moser Institute for Auditory neuroscience and InnerEarLab University Medical Center Göttingen37075 GöttingenGermany Collaborative Research Center 889 University of Göttingen37075 GöttingenGermany Auditory neuroscience and Synaptic nanophysiology Group Max Planck Institute for Multidisciplinary Sciences37075 GöttingenGermany Göttingen Graduate Center for neurosciences Biophysics and Molecular BiosciencesUniversity of Göttingen37075 GöttingenGermany Biochemistry of Membrane Dynamics Group Institute for Auditory NeuroscienceUniversity Medical Center Göttingen37075 GöttingenGermany Molecular Architecture of Synapses Group Institute for Auditory Neuroscience and InnerEarLabUniversity Medical Center Göttingen37075 GöttingenGermany Center for Biostructural Imaging of neurodegeneration University Medical Center Göttingen37075 GöttingenGermany Auditory Systems Physiology Group Institute for Auditory Neuroscience and InnerEarLabUniversity Medical Center Göttingen37075 GöttingenGermany Departamento de Genética e Biologia Evolutiva Centro de Pesquisas sobre o Genoma Humano e Células-TroncoInstituto de BiociênciasUniversidade de São PauloSão Paulo 05508-220Brazil Laboratório de Otorrinolaringologia/LIM32 Faculdade de MedicinaHospital das ClínicasUniversidade de São PauloSão PauloSP 05508-220Brazil DERDIC Pontifícia Universidade Católica de São PauloSão Paulo 05508-220Brazil Department of Molecular neurobiology Max Planck Institute for Multidisciplinary Sciences37075 GöttingenGermany Multiscale Bioimaging Cluster of Excellence(MBExC) University of Göttingen37075 GöttingenGermany Functional Auditory Genomics Group Auditory Neuroscience and Optogenetics LaboratoryGerman Primate Center37077 GöttingenGermany Hearing Genomics Group Institute for Auditory Neuroscience and InnerEarLabUniversity Medical Center Göttingen37075 GöttingenGermany Institute of Human Genetics University Medical Center Göttingen37075 GöttingenGermany

Dear Editor,Afferent synapses of cochlear inner hair cells(IHCs)employ a unique molecular machinery(see extended background in Supplementary Materials).Otoferlin is a key player in this machinery and its defects cause human auditory synaptopathy(Moser and Starr,2016).Otoferlin,a tail-anchored(Vogl et al.,2016)multi-C_(2)-domain protein(Fig.1Ai)specific to hair cells(Roux et al.,2006),is a member of the ferlin protein family involved in membrane trafficking and repair that are of major disease relevance(Pangršičet al.,2012),also see Supplementary *** is distributed broadly within IHCs(Fig.2Ai-Aiii;Pangrsic et al.,2010;Roux et al.,2006).

关键词： auditory hair

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Poly(phenylene oxide) and Renewable Polyamide 11 Blends Compatibilized by Ethylene-n-Octene Copolymer

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Journal of Renewable Materials 2018年第7期6卷 772-783页

作者： regina Jeziorska Agnieszka Abramowicz Agnieszka Szadkowska Anna Pasnik Ewa Spasowka Department of Polymer Technology and Processing Industrial Chemistry Research InstituteRydygiera 801-793 WarsawPoland

Poly(phenylene oxide)/renewable polyamide 11 (PPO/PA11 20/80) blends toughened with glycidylmethacrylate grafted ethylene-n-octene copolymer (GEOC) were prepared in a co-rotating twin-screwextruder. The reaction between GEOC and PPO/PA11 blend was analyzed by gel content tests. Themorphology of PPO/PA11/GEOC blends was observed by scanning electron microscope. The SEMresults showed that PPO formed the continuous phase, though it is a minority component of blends. Withincreasing GEOC content from 5 to 15 wt% the morphology of the blends transformed from droplet-matrix toco-continuous structure, in which both PA11 and PPO phases are continuous. The blend with co-continuousmorphology had better mechanical properties than those with droplet-matrix morphology. The impactstrength of the PPO/PA11/GEOC blends was much higher compared to the one without GEOC as well asPA11 due to the compatibilizing effect, which was also proved by DSC analysis, rheological behavior (MFR,DMTA) and tensile properties.

关键词： Compatibilization elastomer PPO/PA11 blends toughening

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FeedEfficiencyService: An architecture for the comparison of data from multiple studies related to dairy cattle feed efficiency indices

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Information Processing in Agriculture 2022年第3期9卷 378-396页

作者： Heitor Magaldi Linhares regina Braga Wagner Antonio Arbex Mariana Magalhaes Campos Fernanda Campos Jose Maria n.David Victor Stroele Programa de Pos-Graduacao em Ciencia da Computacao Universidade Federal de Juiz de ForaCampus UniversitarioRua JoseLourenco Kelmers/n-Sao PedroJuiz de ForaMinas Gerais 36036-900Brasil Brazilian Agricultural Research Corporation-Embrapa Av.Eugenio do Nascimento610-AeroportoJuiz de Fora-MGMinas Gerais 36038-330Brasil

The increased demand for food worldwide,the reduced land availability for livestock pro-duction,the increasing cost of animal feed and the need for mitigating livestock-related greenhouse gas emissions have driven the search for animal feeding systems that proves more *** tackle this problem,we propose the use of computational support to help researchers compare data on feed efficiency,therefore improving economic and environ-mental *** a solution,we present an integrative architecture capable of combining heterogeneous data from multiple experiments related to dairy cattle feed efficiency *** proposed architecture,called FeedEfficiencyService,classifies animals according to feed efficiency indices and allows visualizations through ontologies and inference *** results obtained from a case study with researchers from the Brazilian Agri-cultural Research Corporation–Dairy Cattle(EMBRAPA)demonstrate that this architecture is a supporting tool in their daily work *** researchers highlighted the importance of the proposed architecture as it allows analyzing animal data,comparing experiments,having reliable data analyses,and standardizing and organizing data from *** novelty of our approach is the use of ontologies and inference engines to enable the discovery of new knowledge and new relationships between data from feed efficiency-related *** store such data,relationships,and analyses of results in an inte-grated *** solution ensures unified access to the processing history and data from diverse experiments,including those conducted at external research centers.

关键词： Dairy Cattle

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Model CAnVAS： Its Application for the Promotion of Regional Economies Under the Case Study of Buffalo Milk Cheese

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Journal of Modern Accounting and Auditing 2018年第4期14卷 174-188页

作者： Martine Joseé Pozo regina Durán national University of Formosa (UnaF) Formosa Argentina University of the Cuenca del Plata (UCP) Corrientes Argentina national University of the South(UnS) Buenos AiresArgentina

The present work proposes a Model CAnVAS （Business Model Canvas）（Osterwalder, 2011） applied to the production and marketing activity of buffalo milk cheese directed to a select market, as a “delicatessen” product. It is evaluated as an alternative to promote the breeding of buffalo cattle, increasing sustainable productivity on lands that are not suitable for other types of activity, in order to promote the economic and social development of subtropical regions and regional economies, emphasizing the strategic study of costs. It is based on a case study of an “entrepreneur” of an agro-food startup located in the province of Formosa （Argentina）. For this purpose, 10 interviews were conducted with customers from the sales office of Santa úrsula S.R.L., inquiring about the perception of the most relevant attributes of the product, arriving at the conclusion that this activity is viable both from a technical and economic perspective.

关键词： CAnVAS buffalo milk cheeses cost strategies regional economies sustainable productivity

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Effects of chronic and subtoxic chlorobenzenes on adrenocorticotrophic hormone release

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Journal of Environmental Sciences 2015年第8期27卷 165-170页

作者： Zsolt Molnár regina Pálf?ldi Anna László Marianna Radács Krisztián Sepp Péter Hausinger László Tiszlavicz Zsuzsanna Valkusz Márta Gálfi Institute of Applied Science Department of Environmental Biology and Education Gyula Juhász Faculty of Education University of Szeged Department of Pulmonology Faculty of Medicine University of Szeged Department of Medical Physics and Informatics Faculty of Medicine University of Szeged Endocrine Unit of First Department of Internal Medicine Faculty of Medicine University of Szeged Invasive Cardiology Department Second Department of Internal Medicine and Cardiology Faculty of Medicine University of Szeged Department of Pathology Faculty of Medicine University of Szeged

Many environmental chemicals and pesticides have been found to alter neuroendocrine communication in exposed biological objects. The environmental loads have primary and secondary effects that can alter the homeostatic regulation potential. Since it is difficult to avoid human exposition, a potentially important area of research to develop in vivo and in vitro experimental models. In this context, the primary aim of this study was to demonstrate the effects of chlorobenzenes on adrenocorticotrophic hormone（ACTH） release. In our experimental study, male Wistar rats were exposed to 0.1, 1.0 and 10 μg/b.w.（body weight） kg of 1,2,4-trichlorobenzene and hexachlorobenzene（Cl B） mix via gastric tube for 30, 60 or 90 days. At the endpoints of the experiment blood samples were taken and animals were decapitated. Primary,monolayer adenohypophysis cell cultures were prepared by enzymatic and mechanical digestion. The ACTH hormone content in serum and supernatant media was measured by immuno-chemiluminescence assay. The Mg2＋-dependent ATPase activity was determined by modified method of Martin and Dotty. Significant differences were detected in the hormone release between the control and treated groups. The hormone release was enhanced characteristically in exposed groups depending upon the dose and duration of exposure. The Mg2＋-ATPase activity enhanced after chronic and subtoxic Cl B exposition. Light microscopy revealed that the adenohypophysis seemed to be more abundant. Results indicate that Wistar rats exposed to subtoxic Cl B have direct and indirect effects on hypothalamus–hypophysis–adrenal axis.

关键词： Chlorobenzenes Subtoxic Adrenocorticotrophic hormone release Mg2+ -dependent ATPase

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Phosphate-activated glutaminase activity is enhanced in brain, intestine and kidneys of rats following portacaval anastomosis

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World Journal of Gastroenterology 2006年第15期12卷 2406-2411页

作者： Manuel Romero-Gómez María Jover Daniel Díaz-Gómez Laura Collantes de Terán regina Rodrigo Inés Camacho Miriam Echevarría Vicente Felipo Juan D Bautista Unit for the Clinical Management of Digestive Diseases Hospital Universitario de Valme SevillaSpain Department of Biochemistry Bromatology and Toxicology Faculty of PharmacyUniversity of SevilleSpain Laboratory of neurobiology Fundación Valenciana de Investigaciones Biomédicas ValenciaSpain Biochemistry Unit Hospital Universitario de Valme SevillaSpain Instituto de Investigaciones Biomédicas Departament of Physiology University of SevilleSpain Laboratory of neurobiology Fundación Valenciana de Investigaciones Biomédicas ValenciaSpain

AIM： To assess whether portacaval anastomosis （PCA） in rats affects the protein expression and/or activity of glutaminase in kidneys, intestines and in three brain areas of cortex, basal ganglia and cerebellum and to explain the neurological alterations found in hepatic encephalopathy （HE）. METHODS： Sixteen male Wistar rats weighing 250-350 g were grouped into sham-operation control （n=8） or portacaval shunt （n = 8）. Twenty-eight days after the procedure, the animals were sacrificed. The duodenum, kidney and brain were removed, homogenised and mitochondria were isolated. Ammonia was measured in brain and blood. Phosphate-activated glutaminase （PAG） activity was determined by measuring ammonia production following incubation for one hour at 37 ℃ with O-phthalaldehyde （OPA） and specific activity expressed in units per gram of protein （pkat/g of protein）. Protein expression was measured by immunoblotting. RESULTS： Duodenal and kidney PAG activities together with protein content were significantly higher in PCA group than in control or sham-operated rats （duodenum PAG activity was 976.95±268.87μkat/g of protein in PCA rats vs 429.19±126.92.μkat/g of protein in shamoperated rats; kidneys PAG activity was 1259.18±228.79 μkat/g protein in PCA rats vs 669.67±400.8 μkat/g of protein in controls, P〈0.05; duodenal protein content： 173% in PCA vs sham-operated rats; in kidneys the content of protein was 152% in PCA vs sham-operated rats）. PAG activity and protein expression in PCA rats were higher in cortex and basal ganglia than those in shamoperated rats （cortex： 6646.6 ±1870.4 μkat/g of protein vs 3573.8± 2037.4 μkat/g of protein in control rats, P〈 0.01; basal ganglia, PAG activity was 3657.3± 1469.6 μkat/g of protein in PCA rats vs 2271.2±384 μkat/g of protein in sham operated rats, P〈0.05; In the cerebellum, the PAG activity was 2471.6±701.4 μkat/g of protein vs 1452.9 ±567.8 μkat/g of protein in the PCA and sham rats, respectively, P〈0.05; content of protein： cerebral co

关键词： Hyperammonaemia Minimal hepaticencephalopathy Glutamine Protein content Shamoperated

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Vitamin D deficiency,functional status,and balance in older adults with osteoarthritis

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World Journal of Clinical Cases 2021年第31期9卷 9491-9499页

作者： Cláudia n Montemor Marcos Tadeu P Fernandes Audrey S Marquez regina Célia Poli-Frederico Rubens Alexandre da Silva Karen B P Fernandes Laboratory of Rehabilitation Research Doctoral Program of Rehabilitation SciencesUniversity Pitágoras Unopar(UNOPAR)Londrina 86041-140ParanaBrazil Department of Anesthesiology Irmandade da Santa Casa de LondrinaLondrina 86010-160ParanaBrazil Health Sciences Research Center University Pitágoras Unopar(UNOPAR)Londrina 86041-140ParanaBrazil Doctoral Program of Rehabilitation Sciences University Pitagoras Unopar(UNOPAR)Londrina 86041-140ParanaBrazil Département des Sciences de la Santé Centre intersectoriel en santédurableLaboratoire de recherche BioNRUniversitédu QuébecàChicoutimi(UQAC)SaguenayG7H 2B1QuébecCanada Centre intégréde santéet services sociaux du Saguenay-Lac-Saint-Jean(CIUSSS SLSJ) Hôpital de La Baie-Services gériatriques spécialisésSaguenayQuébecG7H 7K9Canada

BACKGROUnD Low vitamin D levels are associated with a more severe case of knee osteoarthritis(OA).However,there are few published reports concerning an association between vitamin D deficiency and functional status of individuals with OA and no reports about postural balance in this *** To analyze the relationship between vitamin D deficiency and severity,functional status,and balance in elderly patients with *** In this cross-sectional study,105 elderly patients with hip and knee OA were *** severity was assessed by the Kellgren-Lawrence *** functional status was assessed with the Lequesne *** balance was assessed using a force platform,and center-of-pressure parameters(velocity at anteroposterior and mediolateral axis)were used as the balance *** 25(OH)vitamin D levels were measured using a chemiluminescence *** Most of the patients(mean age:70.6±6.5 years)were female(n=78,74.3%).In the group with vitamin D deficiency,43 patients(56.6%)had severe OA,while 33 patients(43.4%)had mild or moderate OA(χ^(2) test,P=0.04).Patients with vitamin D deficiency showed a higher Lequesne index score(Mann-Whitney test,P=0.04),indicating a worse functional impairment when compared to individuals with normal vitamin D ***,patients with vitamin D deficiency had worse postural balance according to the Mann-Whitney test(P=0.03).COnCLUSIOn Vitamin D deficiency is associated with worse severity,functional status,and postural balance in patients with OA.

关键词： Osteoarthritis Elderly Vitamin D Disability Severity Balance

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Art making and expressive art therapy in adult health and nursing care:A scoping review

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International Journal of nursing Sciences 2021年第1期8卷 102-119,I0007页

作者： Heli Vaartio-Rajalin regina Santamaki-Fischer Pamela Jokisalo Lisbeth Fagerstrom Health Sciences Åbo Akademi UniversityVasaFinland Bachelor of Healthcare/nursing Novia University of Applied SciencesÅboFinland Health Sciences University of South-Eastern NorwayKongsbergNorway

Objectives:While receptive art engagement is known to promote health and wellbeing,active art engagement has not been fully explored in health and nursing *** review is to describe the existing knowledge on art making and expressive art therapy in adult health and nursing care between 2010 and ***:Relevant studies and grey literature were searched and identified between March 17 and April 10,2020 from EBSCO,CInAHL,Medline and ERIC databases and a general Internet *** data charting and extraction,the data(n=42 papers)were summarized and reported in accordance with PRISMA-ScR ***:In the included papers,both art making and expressive art therapy were seen in different health care and nursing contexts:yet not the home care *** emphasis of art activities were group activities for chronically or terminally ill residents,adults aged 65 years or older.A focus on personal narrative was often seen,which may explain why art activities appear to be linked to acknowledging and building new strengths and skills,making meaning of experiences,personal growth,symptom alleviation,and communication;all used to foster collaboration between patients,patients’near-ones and health care ***:Art activities appear to be suitable for every context and can promote personcenteredness and the measurement of nursing outcomes,and they should be considered an essential part of health and nursing care,nursing education and care for health care personnel.

关键词： Adult Aged Art making Art therapy Health care nursing care Person-centered care

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