Effects of chronic and subtoxic chlorobenzenes on adrenocorticotrophic hormone release

作     者：Zsolt Molnár Regina Pálf?ldi Anna László Marianna Radács Krisztián Sepp Péter Hausinger László Tiszlavicz Zsuzsanna Valkusz Márta Gálfi 

作者机构：Institute of Applied Science Department of Environmental Biology and Education Gyula Juhász Faculty of Education University of Szeged Department of Pulmonology Faculty of Medicine University of Szeged Department of Medical Physics and Informatics Faculty of Medicine University of Szeged Endocrine Unit of First Department of Internal Medicine Faculty of Medicine University of Szeged Invasive Cardiology Department Second Department of Internal Medicine and Cardiology Faculty of Medicine University of Szeged Department of Pathology Faculty of Medicine University of Szeged 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2015年第27卷第8期

页      面：165-170页

核心收录：

学科分类：082803[工学-农业生物环境与能源工程] 08[工学] 0828[工学-农业工程] 

基　　金：supported by TáMOP - 4.2.2.A-11/1/KONV - 2012-0047 supported by the European Union and the State of Hungary co-financed by the European Social Fund in the framework of TáMOP 4.2.4.A/2-11-1-2012-0001 ‘ National Excellence Program ’ supported by TáMOP - 4.1.1.C-12/1/KONV - 2012-0012 

主　　题：Chlorobenzenes Subtoxic Adrenocorticotrophic hormone release Mg2+  dependent ATPase 

摘      要：Many environmental chemicals and pesticides have been found to alter neuroendocrine communication in exposed biological objects. The environmental loads have primary and secondary effects that can alter the homeostatic regulation potential. Since it is difficult to avoid human exposition, a potentially important area of research to develop in vivo and in vitro experimental models. In this context, the primary aim of this study was to demonstrate the effects of chlorobenzenes on adrenocorticotrophic hormone（ACTH） release. In our experimental study, male Wistar rats were exposed to 0.1, 1.0 and 10 μg/b.w.（body weight） kg of 1,2,4-trichlorobenzene and hexachlorobenzene（Cl B） mix via gastric tube for 30, 60 or 90 days. At the endpoints of the experiment blood samples were taken and animals were decapitated. Primary,monolayer adenohypophysis cell cultures were prepared by enzymatic and mechanical digestion. The ACTH hormone content in serum and supernatant media was measured by immuno-chemiluminescence assay. The Mg2＋-dependent ATPase activity was determined by modified method of Martin and Dotty. Significant differences were detected in the hormone release between the control and treated groups. The hormone release was enhanced characteristically in exposed groups depending upon the dose and duration of exposure. The Mg2＋-ATPase activity enhanced after chronic and subtoxic Cl B exposition. Light microscopy revealed that the adenohypophysis seemed to be more abundant. Results indicate that Wistar rats exposed to subtoxic Cl B have direct and indirect effects on hypothalamus–hypophysis–adrenal axis.