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Optimization of high throughput spectral flow cytometry for immune cell profiling in mouse liver

Liver Research 2023年第3期7卷 263-271页

作者： Grayson W.Way Hongkun Lu Xuan Wang Derrick Zhao Carmen Camarena Devanand Sarkar rebecca K.martin Huiping Zhou C.Kenneth and Dianne Wright Center for Clinical and Translational Research Virginia Commonwealth UniversityRichmondVAUSA Department of Microbiology and Immunology Virginia Commonwealth University School of MedicineRichmondVAUSA Department of Human and Molecular Genetics Massey Cancer CenterVCU Institute of Molecular Medicine(VIMM)Virginia Commonwealth UniversityRichmondVAUSA Massey Cancer Center Virginia Commonwealth UniversityRichmondVAUSA VCU Institute of Molecular Medicine Virginia Commonwealth UniversityRichmondVAUSA Central Virginia Veterans Healthcare System RichmondVAUSA

The liver plays an important role in both metabolism and *** of the hepatic immune microenvironment is closely associated with various liver *** gain a better understanding of how different types of immune cells contribute to the progression of liver diseases,it is crucial to thoroughly characterize hepatic immune *** direct digestion of liver tissue is a relatively simple method for isolating immune cells,it often induces excessive hepatocyte death,which causes a release of intracellular components that leads to the activation of stress responses and injury in the surrounding *** injury can lead to excessive death in the hepatic immune cells,making isolation and accurate characterization of the immune profile challenging,especially in diseased *** method described here addresses these challenges by utilizing Phosphate buffered saline(PBS)and digestion buffer perfusions to eliminate contaminating blood cells,ensure a pure hepatic immune population,and minimize hepatic immune cell *** ex vivo digestion of the liver enables the isolation of the immune cells from the hepatic tissues and the generation of a single-cell suspension that can be stained for spectral flow *** enhance intracellular cytokine detection and maintain signaling under different physiological and pathological conditions,this protocol uses an in vivo administration of Brefeldin A,a less toxic inhibitor of cytokine *** in vivo administration of Brefeldin A allows for a more accurate representation of the immune cell function and cytokine expression compared to the traditionally used ex vivo Brefeldin A administration.A comprehensive spectral flow cytometry panel,comprising extracellular and intracellular staining,is used for deep immunophenotyping and immune cell effector function *** this protocol is specifically designed for liver digestion of Mdr2 knockout mice(a model for primary sclerosing cholangitis)and f

关键词： metabolism flow utilizing

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Manipulation of photosensory and circadian signaling restricts phenotypic plasticity in response to changing environmental conditions in Arabidopsis

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Molecular Plant 2024年第9期17卷 1458-1471页

作者： martin William Battle Scott Fraser Ewing Cathryn Dickson Joseph Obaje Kristen N.Edgeworth rebecca Bindbeutel Rea LAntoniou-Kourounioti Dmitri A.Nusinow Matthew Alan Jones Plant Science Group School of Molecular BiosciencesUniversity of GlasgowGlasgow G128QQUK Danforth Plant Science Center St.LouisMO 63132USA Department of Biological and Biomedical Sciences Washington University in St.LouisSt.LouisMO 63130USA Present address:School of Biological and Environmental Sciences Liverpool John Moores UniversityLiverpool L22QPUK

Plants exploit phenotypic plasticity to adapt their growth and development to prevailing environmental *** of light and temperature signals is aided by the circadian system,which provides a temporal *** plasticity provides a selective and competitive advantage in nature but is obstructive during large-scale,intensive agricultural practices since economically important traits(including vegetative growth and flowering time)can vary widely depending on local environmental *** prevents accurate prediction of harvesting times and produces a variable *** this study,we sought to restrict phenotypic plasticity and circadian regulation by manipulating signaling systems that govern plants'responses to environmental *** modeling of plant growth and develop-ment predicted reduced plant responses to changing environments when circadian and light signaling pathways were *** tested this prediction by utilizing a constitutively active allele of the plant photoreceptor phytochrome B,along with disruption of the circadian systemvia mutation of *** found that these manipulations produced plants that are less responsive to light and temper-ature cues and thus fail to anticipate *** engineered plants have uniform vegetative growth and flowering time,demonstrating how phenotypic plasticity can be limited while maintaining plant *** has significant implications for future agriculture in both open fields and controlled environments.

关键词： circadian developmental plasticity phenotypic plasticity external coincidence light temperature

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Role of adipocytes in hypertension

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World Journal of Hypertension 2016年第2期6卷 66-75页

作者： rebecca martin Joseph I Shapiro Joan C. Edwards School of Medicine Marshall University

Although it has known for some time that obesity is associated with salt sensitivity and hypertension, recent data suggests that the adipocyte may actually be the proximate cause of this physiological changes. In the following review, the data demonstrating this association as well as the potentially operative pathophysiological mechanisms are reviewed and discussed.

关键词： Hypertension Oxidant stress Natriuresis Heme oxygenase Nitric oxide Renal function Obesity

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新天鹅护理休闲区,本特利,澳大利亚

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世界建筑 2024年第6期 66-71页

作者：无 Adrian Iredale Finn Pedersen Martyn Hook Tom See Hoo rebecca Angus Mary McAree rebecca Hawkett Catherine Dupuy Gian Tonossi Jordan Blagaich Jake Gethin Daniel martin Peter Bennetts(摄影) 伊代尔-佩德森-霍克建筑事务所不详

新天鹅护理休闲区旨在通过减少隔离、鼓励安全的活动和社交互动来改善居民的健康和福祉,并欢迎朋友、家人和周边居民的到来。Iph建筑事务所挑战了之前的总体规划,将车辆和道路从中心空间移除,取而代之的是一条宽阔、笔直且清静的人行林... 详细信息

新天鹅护理休闲区旨在通过减少隔离、鼓励安全的活动和社交互动来改善居民的健康和福祉,并欢迎朋友、家人和周边居民的到来。Iph建筑事务所挑战了之前的总体规划,将车辆和道路从中心空间移除,取而代之的是一条宽阔、笔直且清静的人行林荫道。林荫大道从天鹅护理中心的主要入口街道(阿迪苑)延伸至新的休闲区,并继续通向到远处的老年人关怀设施。一条蜿蜒曲折的小路与林荫大道相接,方便人们慢步闲逛。随着总体规划逐渐深化,这些小路将继续延伸,为老年痴呆症患者提供安全的活动空间。

关键词：护理中心建筑事务所主要入口林荫大道本特利休闲区蜿蜒曲折林荫道

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Pollinators exert positive selection on flower size on urban,but not on rural Scotch broom(Cytisus scoparius ***)

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Journal of Plant Ecology 2018年第3期11卷 493-501页

作者： Robert Frederick Bode rebecca Tong Department of Natural Sciences Saint Martin’s University405 Old Main5000 Abbey WayLaceyWA 98503USA

Aims Adaptive evolution of invasive species is both particularly exciting for the evolutionary biologist and worrisome for those interested in controlling or halting *** species often have a dis-tinct timeline and well-recorded population *** invaders encounter new environments,they undergo rapid adaptive *** aim in this study was to measure variation of floral size in the invasive shrub Cytisus scoparius(Scotch broom)and measure natural selection by pollinators on that *** research has found that this invasive plant is pollinator limited in Washington State and that declines in pollinator populations can contribute to local extinction in another invaded range(New Zealand).This plant is pollinated by both native and introduced species of bees,represent-ing a broad range of pollinator *** scoparius has a flower structure that is highly conducive to studies on pollinator choice,even in the absence of direct pollinator *** We surveyed urban and rural sites in and around the city of Olympia in Washington *** banner width,we were able to show that flower size varies substantially between plants but mini-mally within *** measuring the proportion of flowers that were‘tripped’,we could determine pollinator visitation rates and thus determine the level of selection due to pollinator choice *** Findings We found that *** is under natural selection by pollinators for increased flower ***,such positive natural selection was only seen in urban populations although it was consistent across two flowering *** populations of Scotch broom do not appear to be under selection on flower *** natural selection by pollinators on broom flowers could result in adaptive evolution into a new pollination niche by an invading species.A higher level of variation in broom flowers seen here than seen in previous works in native regions suggests that *** may be high

关键词： invasive evolution pollinator selection flower

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Chemokine receptor 8 expression may be linked to disease severity and elevated interleukin 6 secretion in acute pancreatitis

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World Journal of Gastrointestinal Pathophysiology 2021年第6期12卷 115-133页

作者： Mwangala Nalisa Ekene Emmanuel Nweke martin D Smith Jones Omoshoro-Jones John WS Devar rebecca Metzger Tanya N Augustine Pascaline N Fru Department of Surgery School of Clinical MedicineFaculty of Health SciencesUniversity of the WitwatersrandJohannesburg 2193GautengSouth Africa Department of Surgery Chris Hani Baragwanath Academic HospitalJohannesburg 1864GautengSouth Africa Institut für Immunologie Ludwig-Maximilians-Universität MünchenMünchen 80539Germany School of Anatomical Sciences Faculty of Health ScienceUniversity of the WitwatersrandJohannesburg 2193GautengSouth Africa

BACKGROUND Acute pancreatitis(AP)is an inflammatory disease,which presents with epigastric pain and is clinically diagnosed by amylase and lipase three times the upper limit of *** 2012 Atlanta classification stratifies the severity of AP as one of three risk categories namely,mild AP(MAP),moderately severe AP(MSAP),and severe AP(SAP).Challenges in stratifying AP upon diagnosis suggest that a better understanding of the underlying complex pathophysiology may be *** To identify the role of the chemokine receptor 8(CCR8),expressed by T-helper type-2 Lymphocytes and peritoneal macrophages,and its possible association to Interleukin(IL)-6 and AP *** This study was a prospective case-control study.A total of 40 patients were recruited from the Chris Hani Baragwanath Academic Hospital and the Charlotte Maxeke Johannesburg Academic *** were performed on 29 patients(14 MAP,11 MSAP,and 4 SAP)and 6 healthy controls as part of a preliminary study.A total of 12 mL of blood samples were collected at Day(D)1,3,5,and 7 post epigastric *** multiplex immunoassay panels,real-time polymerase chain reaction(qRT-PCR)arrays,and multicolour flow cytometry analysis,immune response-related proteins,genes,and cells were profiled *** Prism^(TM) software and fold change(FC)analysis was used to determine differences between the groups.P<0.05 was considered *** The concentration of IL-6 was significantly different at D3 post epigastric pain in both the MAP group and MSAP group with P=0.001 and P=0.013 respectively,in a multiplex *** a FC of 2 was applied to identify differentially expressed genes using RT2 Profiler,CCR8 was shown to increase steadily with disease severity from MAP(1.33),MSAP(38.28)to SAP(1172.45)median *** verification studies using RT-PCR showed fold change increases of CCR8 in MSAP and SAP ranging from 1000 to 1000000 times when represented as Log10,compared to healthy co

关键词： Acute Pancreatitis Severity Stratification,Interleukin-6 Chemokine Receptor 8 Lymphocytes Monocytes

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Tropical forest canopies and their relationships with climate and disturbance： results from a global dataset of consistent field-based measurements

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Forest Ecosystems 2018年第1期5卷 77-90页

作者： marion pfeifer alemu gonsamo william woodgate luis cayuela andrew r.marshall alicia ledo timothy c.e.paine rob marchant andrew burt kim calders colin courtney-mustaphi aida cuni-sanchez nicolas j.deere dereje denu jose gonzalez de tanago robin hayward alvaro lau manuel j.macía pieter i.olivier petri pellikka hamidu seki deo shirima rebecca trevithick beatrice wedeux charlotte wheeler pantaleo k.t.munishi thomas martin abdul mustari philip j.platts school of natural and environmental sciences newcastle universityUpon TyneNewcastle NE1 7RUUK department of geography and planning university of torontoONCanada land and water commonwealth scientific and industrial research organisationYarralumlaACTAustralia school of mathematical and geospatial sciences rmit universityGPO Box 2476VMelbourneVIC 3001Australia department of biology geologyphysics and inorganic chemistryuniversidad rey juan carlosE-28933 MostolesMadridSpain tropical forest and peopleresearch centre university of the sunshine coastsippy DownsAustralia environment department university of yorkYorkUK flamingo land ltd. MaltonUK the institute of biological and environmental sciences environmental modelling groupuniversity of aberdeenAberdeenUK biological and environmental sciences university of stirlingStirling FK9 4L AUK york institute for tropical ecosystems environment departmentuniversity of yorkYork YO10 5NGUK department of geography university college londonGower StreetLondon WC1E 6BTUK earth observation climate and optical groupnational physical laboratoryHampton RoadTeddingtonMiddlesex TW11 0LWUK department of archaeology and ancient history uppsala universitetP.O.Box 256-751 05 UppsalaSESweden center for macroecology evolution and climateuniversity of copenhagenUniversitetsparken 152100 CopenhagenDKDenmark durrell institute of conservation and ecology(dice) school of anthropology and conservationuniversity of kentCanterbury CT2 7NRUK department of biology college of natural sciencesjimma universityJimmaEthiopia wageningen university&research laboratory of geo-information science and remote sensingDroevendaalsesteeg 36708 PB Wageningenthe Netherlands center for international forestry research(cifor) Situ GedeSindang BarangBogor 16680Indonesia departamento de biología área de botánicauniversidad autónoma de madridcalle Darwin 2-28049 MadridESSpain conservation ecology research unit university of pretoriaHatfieldPretoriaSouth Af

Background： Canopy structure, defined by leaf area index （LAI）, fractional vegetation cover （FCover） and fraction of absorbed photosynthetically active radiation （fAPAR）, regulates a wide range of forest functions and ecosystem services. Spatially consistent field-measurements of canopy structure are however lacking, particularly for the tropics. Methods： Here, we introduce the Global LAI database： a global dataset of field-based canopy structure measurements spanning tropical forests in four continents （Africa, Asia, Australia and the Americas）. We use these measurements to test for climate dependencies within and across continents, and to test for the potential of anthropogenic disturbance and forest protection to modulate those dependences. Results： Using data collected from 887 tropical forest plots, we show that maximum water deficit, defined across the most arid months of the year, is an important predictor of canopy structure, with all three canopy attributes declining significantly with increasing water deficit. Canopy attributes also increase with minimum temperature, and with the protection of forests according to both active （within protected areas） and passive measures （through topography）. Once protection and continent effects are accounted for, other anthropogenic measures （e.g. human population） do not improve the model. Conclusions： We conclude that canopy structure in the tropics is primarily a consequence of forest adaptation to the maximum water deficits historically experienced within a given region. Climate change, and in particular changes in drought regimes may thus affect forest structure and function, but forest protection may offer some resilience against this effect.

关键词： Leaf area index Fractional vegetation cover Fraction of absorbed photosynthetically active radiation,Human population pressure Protected areas Drought Climate change

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A solution scan of societal options to reduce transmission and spread of respiratory viruses:SARS-CoV-2 as a case study

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Journal of Biosafety and Biosecurity 2021年第2期3卷 84-90页

作者： William J.Sutherland Nigel G.Taylor David C.Aldridge Philip martin Catherine Rhodes Gorm Shackelford Simon Beard Haydn Belfield Andrew J.Bladon Cameron Brick Alec P.Christie Andrew P.Dobson Harriet Downey Amelia S.C.Hood Fangyuan Hua Alice C.Hughes rebecca M.Jarvis Douglas MacFarlane William H.Morgan Anne-ChristineMupepele Stefan J.Marciniak Cassidy Nelson SeánÓhÉigeartaigh Clarissa Rios Rojas Katherine A.Sainsbury rebecca K.Smith Lalitha S.Sundaram Ann Thornton John Watkins Thomas B.White Kate Willott Silviu O.Petrovan BioRISC(Biosecurity Research Initiative at St Catharine’s) St Catharine’s CollegeCambridge CB21RLUK Department of Zoology University of CambridgeThe David Attenborough BuildingPembroke StreetCambridge CB23QZUK Tour du Valat Research Institute for the Conservation of Mediterranean Wetlands13200 ArlesFrance Centre for the Study of Existential Risk University of CambridgeCB21SBUK Department of Social Psychology University of AmsterdamNieuwe Achtergracht 129 B1018 WT AmsterdamNetherlands Department of Ecology and Evolutionary Biology 117 Eno HallPrinceton UniversityNJ 08544USA Institute of Ecology College of Urban and Environmental SciencesPeking UniversityBeijing 100871PR China Centre for Integrative Conservation Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesXishuangbannaYunnan 666303PR China Te Kura Putaiao-School of Science Auckland University of TechnologyAucklandNew Zealand School of Psychological Sciences University of Western AustraliaPerth35 Stirling HwyCrawley WA 6009Australia Nature Conservation and Landscape Ecology Biometry and Environmental System AnalysisUniversity of FreiburgGermany Cambridge Institute for Medical Research University of CambridgeCambridge Biomedical CampusThe Keith Peters BuildingHills RoadCambridge CB20XYUK Future of Humanity Institute University of OxfordOxford OX11PTUK Faculty of Kinesiology Sportand RecreationUniversity of Alberta2-130V Van Vliet 21 Complex(University Hall)EdmontonAlberta T6G 2H9Canada Division of Population Medicine School of MedicineCardiff UniversityCardiff CF144YSUK

Societal biosecurity–measures built into everyday society to minimize risks from pests and diseases–is an important aspect of managing epidemics and *** aimed to identify societal options for reducing the transmission and spread of respiratory *** used SARS-CoV-2(severe acute respiratory syndrome coronavirus 2)as a case study to meet the immediate need to manage the COVID-19 pandemic and eventually transition to more normal societal conditions,and to catalog options for managing similar pandemics in the *** used a‘solution scanning’*** read the literature;consulted psychology,public health,medical,and solution scanning experts;crowd-sourced options using social media;and collated comments on a ***,we present a list of 519 possible measures to reduce SARS-CoV-2 transmission and *** provide a long list of options for policymakers and businesses to consider when designing biosecurity plans to combat SARS-CoV-2 and similar pathogens in the *** also developed an online application to help with this *** encourage testing of actions,documentation of outcomes,revisions to the current list,and the addition of further options.

关键词： Biosecurity Coronavirus COVID-19 Disease control New normal Public health

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Uptake and mitochondrial dysfunction of alpha-synuclein in human astrocytes, cortical neurons and fibroblasts

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Translational Neurodegeneration 2013年第1期2卷 134-142页

作者： Nady Braidy Wei-Ping Gai Ying Hua Xu Perminder Sachdev Gilles J Guillemin Xing-Mai Jiang J William O Ballard martin P Horan Zhi Ming Fang Beng H Chong Daniel Kam Yin Chan Centre for Healthy Brain Ageing School of PsychiatryUniversity of New South WalesSydneyAustralia Aged Care and Rehabilitation Bankstown-Lidcombe HospitalSydneyAustralia Department of Human Physiology and Centre for Neuroscience Flinders University School of MedicineAdelaideAustralia Faculty of Medicine University of New South WalesSydneyAustralia Neuropsychiatric Institute Prince of Wales HospitalSydneyAustralia School of Medical Sciences University of New South WalesSydneyAustralia St Vincent’s Centre for Applied Medical Research SydneyAustralia St George Clinical School University of New South Wales and St George HospitalSydneyAustralia School of Biotechnology and Biomolecular Sciences University of New South WalesSydneyAustralia Aged Care and Rehabilitation Unit Bankstown-Lidcombe HospitalEldridge RoadBankstown 2031NSWAustralia

The accumulation and aggregation of alpha-synuclein(α-syn)in several tissue including the brain is a major pathological hallmark in Parkinson’s disease(PD).In this study,we show that α-syn can be taken up by primary human cortical neurons,astrocytes and skin-derived fibroblasts in *** findings that brain and peripheral cells exposed to α-syn can lead to impaired mitochondrial function,leading to cellular degeneration and cell death,provides additional evidence for the involvement of mitochondrial dysfunction as a mechanism of toxicity of α-syn in human cells.

关键词： Alpha-synuclein Neurons Astrocytes Fibroblasts Mitochondria

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药剂车

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世界建筑导报 2021年第5期36卷 48-48页

作者： Emilie Taylor Welty Nick Jenisch Elizabeth Bateman Jeremy Baudy Anna Deeg Claire Divito rebecca Dunn Adrian Evans Danelle martin Danielle Scheeringa Bhumika Shirole Zach Speroni James Rennert Dana Ridenour 不详

设计如何开启对话,并作为宣传和教育的工具?药剂车,许多移动的装有药剂的车,试图让我们想象没有监狱的景象。美国有220万犯人,约9万人无限期单独监禁。疏离、人性丧失、绝望、迷失方向、偏执、自杀意念,单独监禁带来的无法弥补,近乎毁... 详细信息

设计如何开启对话,并作为宣传和教育的工具?药剂车,许多移动的装有药剂的车,试图让我们想象没有监狱的景象。美国有220万犯人,约9万人无限期单独监禁。疏离、人性丧失、绝望、迷失方向、偏执、自杀意念,单独监禁带来的无法弥补,近乎毁灭的影响远不止这些。囚犯药剂车从受大规模监禁荼毒最深的社区采取植物,转化为药。药剂师根据交谈或书信为全美国单独监禁犯人提供天然药物、茶、酊剂、蒸汽和药膏。

关键词：单独监禁自杀意念天然药物监狱药剂师犯人

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