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Heterologous expression of bacteriocin E-760 in Chlamydomonas reinhardtii and functional analysis

Phyton-International Journal of Experimental Botany 2019年第1期88卷 25-35页

作者： Quezada-Rivera JJ re soria-guerra FS Pérez-Juárez L Martínez-González SE Valdés-Rodríguez NL Vasco-Méndez JF Morales-Domínguez Departamento de Química Universidad Autónoma de AguascalientesUniversidad No.940Ciudad UniversitariaC.P.20131AguascalientesAgs.México Facultad de Ciencias Químicas Universidad Autónoma de San Luis PotosíZona Universitaria PonienteC.P.78290San Luis PotosíS.L.P.México Departamento de Biotecnología y Bioquímica Centro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalCinvestav-Unidad Irapuato.Km.9.6 Libramiento Norte Carr.Irapuato-León 36824 IrapuatoGto.México

The use of antimicrobial peptides(AMPs)synthesized by bacteria(bacteriocins)is an alternative for combating multidrug resistant bacterial strains and their production by recombinant route is a viable option for their mass *** bacteriocin E-760 isolated from the genus Enterococcus *** been shown to possess inhibitory activity against Gram-negative and Gram-positive *** this study,the expression of a chimeric protein coding for E-760 in the nucleus of *** was evaluated,as well as,its antibacterial *** synthetic gene E-760S was inserted into the genome of *** using Agrobacterium tumefaciens.A transgenic line was identified in TAP medium with hygromycin and also by *** increment in the culture medium temperature of the transgenic strain at 35°C for 10 minutes,increased the production level of the recombinant protein from 0.14(Noninduced culture,NIC)to 0.36%(Induced culture,IC)of total soluble proteins(TSP);this was quantified by an ELISA *** E-760 possesses activity against Staphylococcus aureus in 0.34 U log,Streptococcus agalactiae in 0.48 U log,Enterococcus faecium in 0.36 U log,Pseudomonas aeruginosa in 2 U log and for Klebsiella pneumoniae,the activity was 0.07 U *** results demonstrate that the nucleus transformation of *** can function as a stable expression platform for the production of the synthetic gene E-760 and it can potentially be used as an antibacterial agent.

关键词： Heterologous expression Antibacterial activity Bacteriocin E-760 Chlamydomonas reinhardtii Log inactivation Nuclear transformation

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Integrating Levels of Hierarchical Organization in Porous Organic Molecular Materials

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Nano-Micro Letters 2024年第5期16卷 134-153页

作者： Jesus Ferrando‑soria Antonio Fernandez Instituto de Ciencia Molecular(ICMol) Universidad de Valencia46980 ValenciaSpain School of Science Loughborough UniversityLoughborough LE113TUUK

Porous organic molecular materials(POMMs)are an emergent class of molecular-based materials characterized by the formation of extended porous frameworks,mainly held by non-covalent *** represent a variety of chemical families,such as hydrogen-bonded organic frameworks,porous organic salts,porous organic cages,C-H···πmicroporous crystals,supramolecular organic frameworks,π-organic frameworks,halogen-bonded organic framework,and intrinsically porous molecular *** some porous materials such as zeolites and metal organic frameworks,the integration of multiscale has been adopted to build materials with multifunctionality and optimized ***,considering the significant role of hierarchy in porous materials and the growing importance of POMMs in the realm of synthetic porous materials,we consider it appropriate to dedicate for the first time a critical review covering both ***,we will provide a summary of literature examples showcasing hierarchical POMMs,with a focus on their main synthetic approaches,applications,and the advantages brought forth by introducing hierarchy.

关键词： Porous organic molecular materials Hierarchy Hydrogen-bonded organic frameworks Porous cages Fullerene

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Benzyl Amino Purine and Gibberellic Acid Coupled to Nitrogen-Limited Stress Induce Fatty Acids,Biomass Accumulation,and Gene Expression in Scenedesmus Obliquus

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Phyton-International Journal of Experimental Botany 2021年第2期90卷 515-531页

作者： Hans Christian Correa-Aguado Gloria Viviana Cerrillo-Rojas Alejandro Rocha-Uribe Ruth Elena soria-guerra JoséFrancisco Morales-Domínguez Departamento de Química Centro de Ciencias BásicasUniversidad Autónoma de AguascalientesAguascalientes20131México Facultad de Ciencias Químicas Universidad Autónoma de San Luis PotosíSan Luis Potosí78210México Instituto Politécnico Nacional Unidad Profesional Interdisciplinaria de Ingeniería Campus ZacatecasBlvd.del Bote 202Cerro del Gato Ejido La EscondidaCol.Ciudad AdministrativaZacatecasZAC 98160Mexico

The need for renewable energy sources makes microalgae an essential feedstock for biofuels *** molecular aspects and the response to nitrogen(N)-limited conditions with a phytohormone stimulus in microalgae have been slightly *** this work,Scenedesmus obliquus was used as a study model to analyze the effect of benzyl amino purine(BAP)and gibberellic acid(GA)coupled to nitrogen limitation on cell growth,biomass and fatty *** selected 10^(-5)M BAP increased the biomass by 1.44-fold,and 10^(-6)M GA by *** total lipids also increased by 2.8 and 1.11-fold,*** 10^(-5)M BAP and 10^(-6)M GA addition to *** cultures at different initial nitrogen percentages(N-0,N-25,and N-50)showed a significant increase in cell growth and biomass productivity compared to the unstimulated *** N-0 and GA N-0 produced the highest lipid yields with 55%and 50%,*** lipid profile analysis revealed an increase,particularly in C18:1 and C16:0 fatty *** expression analysis showed an over-expression of acyl carrying protein(ACP),stearoyl-ACP desaturase(SAD),fatty acid acyl-ACP thioesterase(FATA),and diacylglycerol acyltransferase(DGAT)genes,which were mainly induced by nitrogen ***,BAP and GA produced a significant over-expression on these genes in the N-replete *** study shows that BAP and GA,coupled to N limitation stress,can be used to increase the biomass and lipid production in *** for sustainable biofuels.

关键词： Biodiesel gene expression lipids microalgae nitrogen deficiency phytohormones

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On the Maximal Operator Associated with Certain Rotational Invariant Measures

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Acta Mathematica Sinica,English Series 2010年第6期26卷 993-1004页

作者： Adrián INFANTE Fernando soria Department of Mathematics Universidad Simn Bolívar Department of Mathematics Universidad Autnoma de Madrid

The aim of this work is to investigate the integrability properties of the maximal operator Mu,associated with a non-doubling measure μ defined on Rn. We start by establishing for a wide class of radial and increasing measures μ that Mu is bounded on all the spaces Lu^p（R^n）,P〉***,we show that there is a radial and increasing measure p for which Mμ does not map Lμ^p（R^n） into weak Lμ^p（R^n）,1≤p〈∞.

关键词： maximal operator non-doubling measures

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Heuristics of node selection criteria to assess robustness of world airport network

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Chinese Journal of Aeronautics 2017年第4期30卷 1473-1480页

作者： Manel soria Oriol LORDAN JosMaría SALLAN Department of Physics Aeronautics Engineering Universitat Politecnica de Catalunya-BarcelonaTech Terrassa 08222 Spain Department of Management Universitat Politecnica de Catalunya-BarcelonaTech Terrassa 08222 Spain

The world airport network（WAN） is one of the networked infrastructures that shape today＇s economic and social activity, so its resilience against incidents affecting the WAN is an important problem. In this paper, the robustness of air route networks is extended by defining and testing several heuristics to define selection criteria to detect the critical nodes of the *** addition to heuristics based on genetic algorithms and simulated annealing, custom heuristics based on node damage and node betweenness are defined. The most effective heuristic is a multiattack heuristic combining both custom heuristics. results obtained are of importance not only for advance in the understanding of the structure of complex networks, but also for critical node detection.

关键词： Adaptive strategy Air route networks Complex networks Intentional attacks Network robustness

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In Vitro Propagation, Isolation and Expression Studies of Suaeda edulis Genes Involved in the Osmoprotectants Biosynthesis

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Phyton-International Journal of Experimental Botany 2020年第3期89卷 715-726页

作者： Gloria Viviana Cerrillo-Rojas Mariana Tiscareno-Andrade Ana Erika Ochoa-Alfaro Eugenio Perez-Molphe Balch Ruth Elena soria-guerra Jose Francisco Morales-Dominguez Departamento de Quimica Universidad Autonoma de AguascalientesCentro de Ciencias BasicasCiudad UniversitariaAguascalientes20131Mexico Facultad de Quimica Universidad Autonoma de San Luis PotosiZona UniversitariaSan Luis Potosi78210Mexico

Halophytes are an excellent choice for the study of genes conferring salt tolerance to salt-sensitive plants and,they are suitable for reclamation and remediation of saline *** develop an in vitro plant propagation protocol and studies of genes involved with GB and Pro biosynthesis in Suaeda *** buds were used as explants and cultured in different treatments on Murashige and Skoog(MS)medium supplemented with different concentrations and combinations of plant growth *** highest number of multiple shoots was on MS medium containing 1 mg/L Benzyladenine(BA)and/or 2 g/L activated carbon with 5.5±06 shoots per *** identification and expression analysis of genes involved in glycine betaine(GB)biosynthesis were S-adenosyl-methionine synthetase(SAMS),choline monooxygenase(CMO)and betaine alde-hyde dehydrogenase(BADH),and for proline(Pro)was pyrroline 5-carboxylate synthetase(P5CS).These sequences shared 90–95%of identity with others plant homologous in public *** amino acids sequence analysis showed that all these peptides contain some of the conserved motifs of those kinds of *** qRT-PCR analysis revealed a higher expression of SeBADH,SeCMO,and,SeP5CS genes in the roots and leaves from plants collected in the field in contrast with from in vitro ***,the expression level of SeSAMS was higher only in the leaves of plants collected in the field when com-pared to those cultivated in vitro.

关键词： Axillary buds halophyte proline glycine betaine salt-resistant

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Photodegradation and removal of Diclofenac by the Green Alga Nannochloropsis oculata

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Phyton-International Journal of Experimental Botany 2021年第5期90卷 1519-1533页

作者： Danaé Samara Sánchez-Sandoval Omar González-Ortega Mara Fernanda Navarro-Martínez Juan Marcos Castro-Tapia Ramón Fernando García de la Cruz Ruth Elena soria-guerra Facultad de Ciencias Químicas Universidad Autónoma de San Luis PotosíSan Luis Potosí78210México

The emerging contaminants(ECs)are organic compounds including *** products are consumed in large quantities,favoring their continuous entrance to the environment and affecting the health of living *** is a non-steroidal anti-inflammatory drug with analgesic properties of prolonged *** is the commonest postoperative analgesic,and it is highly consumed without *** recent years,the capability of microalgae to remove pharmaceuticals in water has been tested as a promising *** this work,the removal efficiency of diclofenac(16µg/mL)by the microalga Nannochloropsis oculata CCAP 186/7 was *** major nutrient concentrations in Bold’s Basal Medium were modified(nitrogen and carbon:50 and 100%of their original concentration)to know their effect in the removal of *** degradation by light was also *** maximum removal capacity of diclofenac in the medium was 68.8%.The percentage of diclofenac adsorbed on the cell wall was between 6 and 12.7%.Around 23.6%of diclofenac was degraded by light after 18 *** did not show growth inhibition due to the presence of *** results obtained point to the promissory application of *** as a bioremediation system for diclofenac removal.

关键词： Adsorption emerging contaminants microalgae pharmaceuticals phycoremediation photodegradation

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Influence of wire spacing and plate spacing on electrostatic precipitation of nanoparticles: An approach involving electrostatic shielding and diffusion charging

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Particuology 2023年第9期80卷 127-139页

作者： Felipe de Aquino Lima Vádila Giovana guerra Department of Chemical Engineering Federal University of São CarlosRodovia Washington LuísKm 235C.P.676CEP 13560-970São CarlosSPBrazil

Electrostatic precipitation is a process widely used as gas cleaning device,to removal particles from gas ***,in a conventional and well-sized precipitator,the collection efficiency decreases for ultrafine particles,making it difficult to employ this equipment for controlling nanoparticle *** paper investigates the influence of plate spacing(4 and 6.5 cm)and wire spacing(4,6,and 12 cm)on the electric current and nanoparticle collection efficiency,considering the effect of diffusion charging and electrostatic *** laboratory-scale dry wire-plate electrostatic precipitators with different plate spacings were tested for the collection of nanoparticles(6.15–241.4 nm)at three air velocities(1.9,2.9,and 3.9 cm/s).The results demonstrated the effectiveness of the equipment in removing nanoparticles(99.9%)under the highest electric *** values of the wire spacing led to increases in the current and the collection *** was associated with reduced electrostatic shielding,which is more evident in smaller ducts with a higher density of field *** is expected that the findings should improve knowledge on electrostatic precipitation of nanoparticles,enabling optimization of collection efficiency by considering the effects of geometric parameters.

关键词： Electrostatic precipitation Nanoparticles Collection efficiency Plate spacing Wire spacing

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Excitotoxicity-induced endocytosis as a potential target for stroke neuroprotection

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Neural regeneration research 2021年第2期16卷 300-301页

作者： Margarita Díaz-guerra Instituto de Investigaciones Biomédicas“Alberto Sols” Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid(CSIC-UAM)MadridSpain

Decreased neuronal survival-signaling and brain damage:Stroke is a leading cause of death worldwide,the major cause of adult disability and second of *** spite of the social and economic importance of this disorder,an... 详细信息

关键词： drugs death damage

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Probing the nature of the contact between fine particles by using ultrasound propagation

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Particuology 2011年第6期9卷 659-662页

作者： Antonio Castellanos Xiaoping Jia Carlos soria-Hoyo Jose Manuel Valverde Faculty of Physics University of Seville Avda. Reina Mercedes s/n 41012 Seville Spain LPMDI Universite de Marne-la-Vallee CNRS UMR 8108 Champs sur Marne France

The propagation velocity （Vs） of an ultrasonic signal through a granular material depends on the type of interparticle contact. For noncohesive glass beads, a power law behavior Vs α σc1/6 for consolidation stresses applied （σc） above 1 MPa has been measured in previous work. This equation is compatible with Hertz＇s interaction law between elastic solids. In the present work, we have tested the propagation velocity of ultrasound signals through a sample of fine powder. The tensile strength and compactivity of the powder were previously measured by means of the Seville powder tester （SPT）, indicating plastic deformation of the surface asperities in contact for small to moderate consolidation stresses. However, the measurements of ultrasound propagation at high consolidations presented here are compatible with Hertz＇s law. This finding suggests that for high consolidation stresses, surface asperities are flattened, and it is therefore the elastic deformation of the bulk of the particles that determines the transmission of ultrasonic pulses.

关键词： JFine cohesive powder Ultrasound propagation Interparticle contact

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