In Vitro Propagation, Isolation and Expression Studies of Suaeda edulis Genes Involved in the Osmoprotectants Biosynthesis
作者机构：Departamento de QuimicaUniversidad Autonoma de AguascalientesCentro de Ciencias BasicasCiudad UniversitariaAguascalientes20131Mexico Facultad de QuimicaUniversidad Autonoma de San Luis PotosiZona UniversitariaSan Luis Potosi78210Mexico
出 版 物：《Phyton-International Journal of Experimental Botany》 (国际实验植物学杂志（英文）)
年 卷 期：2020年第89卷第3期
页 面：715-726页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：This research was financially supported by Universidad Autonoma de Aguascalientes(PIBT18-1) Consejo Nacional de Ciencia y Tecnologia(174795)
主 题：Axillary buds halophyte proline glycine betaine salt-resistant
摘 要：Halophytes are an excellent choice for the study of genes conferring salt tolerance to salt-sensitive plants and,they are suitable for reclamation and remediation of saline *** develop an in vitro plant propagation protocol and studies of genes involved with GB and Pro biosynthesis in Suaeda *** buds were used as explants and cultured in different treatments on Murashige and Skoog(MS)medium supplemented with different concentrations and combinations of plant growth *** highest number of multiple shoots was on MS medium containing 1 mg/L Benzyladenine(BA)and/or 2 g/L activated carbon with 5.5±06 shoots per *** identification and expression analysis of genes involved in glycine betaine(GB)biosynthesis were S-adenosyl-methionine synthetase(SAMS),choline monooxygenase(CMO)and betaine alde-hyde dehydrogenase(BADH),and for proline(Pro)was pyrroline 5-carboxylate synthetase(P5CS).These sequences shared 90–95%of identity with others plant homologous in public *** amino acids sequence analysis showed that all these peptides contain some of the conserved motifs of those kinds of *** qRT-PCR analysis revealed a higher expression of SeBADH,SeCMO,and,SeP5CS genes in the roots and leaves from plants collected in the field in contrast with from in vitro ***,the expression level of SeSAMS was higher only in the leaves of plants collected in the field when com-pared to those cultivated in vitro.
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