检索结果-南通市图书馆

Using single cell transcriptomics to study the complexity of human retina

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2020年第11期15卷 2045-2046页

作者： Daniel Urrutia-Cabrera raymond Ching-Bong wong Centre for Eye Research Australia Department of Surgery(Ophthalmology)University of MelbourneMelbourneAustralia

The human retina is a specialized multilayered structure composed of numerous cell *** process of vision relies on a robust network integrated by rod photoreceptors,cone photoreceptors,bipolar cells,horizontal cells,amacrine cells and retinal ganglion cells,which detect,process and relay the visual information to the ***,structural and metabolic support is provided by Muller glia,retinal astrocytes and *** 200 genes have been implicated in inherited retinal diseases(RetNet:https://***/retnet/).However,in many cases,the retinal cell types that express these disease-associated genes remain to be *** complexity of the human retina represents a major challenge for the molecular profiling of all retinal cell *** previous studies utilised bulk RNA-seq to profile the whole human adult retina,which only analysed the averaged gene expression levels across all retinal cell *** such,knowledge of the transcriptome profile in specific cell types within the retina would help us to unravel the heterogeneity of retinal cells,advance understanding of the pathogenesis of inherited retinal diseases,and to develop gene therapies that could improve treatment options.

关键词： diseases specialized retina

Neuroregeneration using in vivo cellular reprogramming

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2017年第7期12卷 1073-1074页

作者： Tu Nguyen raymond Ching-Bong wong Centre for Eye Research Australia East Melbourne VIC Australia Ophthalmology Department of Surgery University of MelbourneMelbourne VIC Australia

Cellular reprogramming is an innovative technology used to artificlally convert a mature cell type into a different cell type by molecular＇manipulation. The general concept of cellular reprogramming is to use master transcription factors to override the endogenous transcriptome profile of a given cell type with the transcriptome profile of the target cell type, thereby altering the cellular function and identity.

关键词： in on cell gene Neuroregeneration using in vivo cellular reprogramming into

An efficient method for privacy preserving location queries

在线全文

学校读者我要写书评

暂无评论

Frontiers of Computer Science 2012年第4期6卷 409-420页

作者： Yubao LIU Xiuwei CHEN Zhan LI Zhijie LI raymond Chi-Wing wong Department of Computer Science Sun Yat-Sen UniversityGuangzhou 510006China Department of Computer Science Jinan UniversityGuangzhou 510632China Department of Computer Science and Engineering The Hong Kong University of Science and TechnologyHong KongChina

Recently, the issue of privacy preserving loca- tion queries has attracted much research. However, there are few works focusing on the tradeoff between location privacy preservation and location query information collection. To tackle this kind of tradeoff, we propose the privacy persevering location query （PLQ）, an efficient privacy pre-serving location query processing framework. This frame- work can enable the location-based query without revealing user location information. The framework can also facilitate location-based service providers to collect some information about the location based query, which is useful in practice. PLQ consists of three key components, namely, the location anonymizer at the client side, the privacy query processor at the server side, and an additional trusted third party connect- ing the client and server. The location anonymizer blurs the user location into a cloaked area based on a map-hierarchy. The map-hierarchy contains accurate regions that are parti- tioned according to real landforms. The privacy query pro- cessor deals with the requested nearest-neighbor （NN） loca- tion based query. A new convex hull of polygon （CHP） algo- rithm is proposed for nearest-neighbor queries using a poly- gon cloaked area. The experimental results show that our al- gorithms can efficiently process location based queries.

关键词： location based service (LBS) privacy preserva- tion nearest-neighbor (NN) location query

Optimal location query based on k nearest neighbours

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Computer Science 2021年第2期15卷 105-117页

作者： Yubao LIU Zitong CHEN Ada Wai-Chee FU raymond Chi-Wing wong Genan DAI School of Data and Computer Science Sun Yat-Sen UniversityGuangzhou510006China Guangdong Key Laboratory of Big Data Analysis and Processing Guangzhou510006China Department of Computer Science and Engineering Chinese University of Hong KongHong Kong999077China Department of Computer Science and Engineering The Hong Kong University of Science and TechnologyHong Kong999077China

Optimal location query in road networks is a basic operation in the location intelligence *** a set of clients and servers on a road network,the purpose of optimal location query is to obtain a location for a new server,so that a certain objective function calculated based on the locations of clients and servers is *** works assume no labels for servers and that a client only visits the nearest *** assumptions are not realistic and it renders the existing work not useful in many *** this paper,we relax these assumptions and consider the k nearest neighbours(KNN)of *** introduce the problem of KNN-based optimal location query(KOLQ)which considers the k nearest servers of clients and labeled *** also introduce a variant problem called relocation KOLQ(RKOLQ)which aims at relocating an existing server to an optimal *** main analysis algorithms are proposed for these *** experiments on the real road networks illustrate the efficiency of our proposed solutions.

关键词： optimal location query k nearest neighbours road network

Hollow silica reinforced magnesium nanocomposites with enhanced mechanical and biological properties with computational modeling analysis for mandibular reconstruction

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Oral Science 2020年第4期12卷 298-308页

作者： Somasundaram Prasadh Vyasaraj Manakari Gururaj Parande raymond Chung Wen wong Manoj Gupta Faculty of Dentistry National University of Singapore9 Lower Kent Ridge RoadSingaporeSingapore Department of Mechanical Engineering National University of Singapore9 Engineering Drive 1SingaporeSingapore

The present study investigates Mg-SiO2 nanocomposites as biodegradable implants for orthopedic and maxillofacial *** effect of presence and progressive addition of hollow silica nanoparticles(0.5,1,and 1.5)vol.%on the microstructural,mechanical,degradation,and biocompatibility response of pure Mg were *** suggest that the increased addition of hollow silica nanoparticles resulted in a progressive increase in yield strength and ultimate compressive strength with Mg-1.5 vol.%SiO2 exhibiting superior *** response of Mg-SiO2 nanocomposites under the influence of Hanks’balanced salt solution revealed that the synthesized composites revealed lower corrosion rates,indicating rapid dynamic passivation when compared with pure ***,cell adhesion and proliferation of osteoblast cells were noticeably higher than pure Mg with the addition of 1 vol.%SiO2 *** biocompatibility and the in vitro biodegradation of the Mg-SiO2 nanocomposites were influenced by the SiO2 content in pure Mg with Mg-0.5 vol.%SiO2 nanocomposite exhibiting the best corrosion resistance and biocompatibility when compared with other *** in mechanical,corrosion,and biocompatibility characteristics of Mg-SiO2 nanocomposites developed in this study are also compared with properties of other metallic biomaterials used in alloplastic mandibular reconstruction in a computational model.

关键词： corrosion magnesium properties

Molecular Advances in Severe Acute Respiratory Syndrome-associated Coronavirus (SARS-CoV)

在线全文

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2003年第4期1卷 247-262页

作者： raymond Kin Hi Hui raymond Tsz Yeung wong Chi Wai Yip Frederick Chi Ching Leung Department of Zoology The University of Hong Kong Hong Kong SAR China.Department of Zoology The University of Hong Kong Hong Kong SAR China.Department of Zoology The University of Hong Kong Hong Kong SAR China.Department of Zoology The University of Hong Kong Hong Kong SAR China.

The sudden outbreak of severe acute respiratory syndrome (SARS) in 2002 prompted the establishment of a global scientific network subsuming most of the traditional rivalries in the competitive field of virology. Within months of the SARS outbreak, collaborative work revealed the identity of the disastrous pathogen as SARS-associated coronavirus (SARS-CoV). However, although the rapid identifi-

关键词： severe acute respiratory syndrome SARS-CoV genome phylogenetics human leukocyte antigen (HLA) system molecular epidemiology

Using transcription factors for direct reprogramming of neurons in vitro

在线全文

学校读者我要写书评

暂无评论

World Journal of Stem Cells 2019年第7期11卷 431-444页

作者： Layal El Wazan Daniel Urrutia-Cabrera raymond Ching-Bong wong Cellular Reprogramming Unit Centre for Eye Research AustraliaMelbourne 3004Australia Department of Surgery University of MelbourneMelbourne 3002Australia Ophthalmology Shenzhen Eye HospitalShenzhen 518040Guangdong ProvinceChina

Cell therapy offers great promises in replacing the neurons lost due to neurodegenerative diseases or ***,a key challenge is the cellular source for transplantation which is often limited by donor *** reprogramming provides an exciting avenue to generate specialized neuron subtypes in vitro,which have the potential to be used for autologous transplantation,as well as generation of patient-specific disease models in the lab for drug discovery and testing gene *** we present a detailed review on transcription factors that promote direct reprogramming of specific neuronal subtypes with particular focus on glutamatergic,GABAergic,dopaminergic,sensory and retinal *** will discuss the developmental role of master transcriptional regulators and specification factors for neuronal subtypes,and summarize their use in promoting direct reprogramming into different neuronal ***,we will discuss up-and-coming technologies that advance the cell reprogramming field,including the use of computational prediction of reprogramming factors,opportunity of cellular reprogramming using small chemicals and microRNA,as well as the exciting potential for applying direct reprogramming in vivo as a novel approach to promote neuro-regeneration within the ***,we will highlight the clinical potential of direct reprogramming and discuss the hurdles that need to be overcome for clinical translation.

关键词： Cell reprogramming Neuronal subtypes Transcription factors Direct reprogramming Glutamatergic neurons GABAergic neurons Retinal neurons

Mesenchymal stromal cell-derived small extracellular vesicles modulate macrophage polarization and enhance angio-osteogenesis to promote bone healing

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genes & Diseases 2022年第4期9卷 841-844页

作者： Shang Jiunn Chuah Chee Weng Yong Kristeen Ye Wen Teo Jacob Ren Jie Chew Yi Ann Cheow Shipin Zhang raymond Chung Wen wong Asher Ah Tong Lim Sai Kiang Lim Wei Seong Toh Faculty of Dentistry National University of Singapore119085Singapore Institute of Molecular and Cell Biology Agency for ScienceTechnology and Research138648Singapore Department of Orthopaedic Surgery Yong Loo Lin School of MedicineNational University of Singapore119288Singapore Department of Biomedical Engineering Faculty of EngineeringNational University of Singapore117583Singapore Tissue Engineering Program Life Sciences InstituteNational University of Singapore117456Singapore Integrative Sciences and Engineering Program NUS Graduate SchoolNational University of Singapore119077Singapore

Mesenchymal stem/stromal cells(MSCs)have demonstrated therapeutic efficacy for bone regeneration in animal and clinical *** MSCs were initially thought to differentiate to various cell types to replace the injured/diseased tissue,it is now accepted that these cells secrete factors to promote tissue repair.1 Among these factors,small extracellular vesicles(sEVs)of size 50–200 nm,which include the exosomes,have been identified as the principal agent mediating the wide-ranging therapeutic efficacy of MSCs.2 Several studies have also reported the therapeutic effects of MSC-sEVs to enhance bone repair in animal models,as recently reviewed.3 However,the cellular processes and mechanisms mediated by MSC-sEVs in bone regeneration remain to be fully elucidated.

关键词： healing clinical macrophage

Impact of Gender,Family Factors and Exploratory Activities on Students’Career and Educational Search Competencies in Shanghai and Hong Kong

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

ECNU Review of Education 2018年第3期1卷 96-115页

作者： Esther Sui-Chu Ho Kwok Wing Sum raymond Sin Kwok wong The Chinese University of Hong Kong University of California Santa Barbara

Purpose-This study examines the career and educational search competencies(CESC),a capability which may be necessary for a successful transition from high school to work or postsecondary education,of students from Shanghai and Hong ***/Approach/Methods-The data for this study was taken from the Main Study of PISA 2012 in Shanghai and Hong *** analysis was used to examine the relative impact of different forms of career and educational exploratory activities on students’***-Results showed a consistent pattern of socio-economic inequality in student’s self-reported CESC in the two Chinese cities,which was largely mediated by the family capital or ***,career and educational exploratory activities initiated by schools,enterprises or the students themselves were found to have significant positive associations with CESC regardless of socioeconomic ***/Value-The paper provides empirical evidence for enhancing students’career search capacity through engaging in career exploration in the face of structural *** light of this,the roles of schools,business sectors,and governments in students’capacity building are discussed.

关键词： Educational and career search educational inequality family capital PISA Shanghai Hong Kong