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The ILux^(®) compared to the mechanical meibomian gland expression for the treatment of moderate and severe meibomian gland dysfunction

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International Journal of Ophthalmology(English edition) 2024年第6期17卷 1042-1048页

作者： María Ximena Núñez Andrea Acosta-Ortega Guillermo raul Vera-Duarte Catalina Gómez-Duarte Unit of Cornea Cataract and Refractive SurgeryVision Sana Research GroupClínica de Oftalmología de CaliFaculty of Medicine Pontificia Universidad Javeriana CaliCali 760031Colombia Department of Ophthalmology Faculty of Medicine Pontificia Universidad Javeriana CaliCali 760031Colombia Department of Ophthalmology Hospital de ClínicasNational University of AsunciónAsunción 111421Paraguay

AIM:To compare the safety and effectiveness of eyelid treatment with the ILux®-MGD Treatment System in one session versus five sessions of mechanical meibomian gland expression(MMGE)in patients with moderate to severe meibomian gland dysfunction(MGD).METHODS:A prospective,randomized,open-label,and controlled clinical trial that compared one session of the ILux®MGD Treatment System versus five sessions of MMGE in both eyes of 130 patients aged≥18y with Ocular Surface Disease Index(OSDI)scores≥13,total meibomian gland scores(MGS)of<15 in the lower eyelid of each eye,and non-invasive tear break-up time(NI-TBUT)<10s,who were randomized 1:1 to ILux®or MMGE.RESULTS:The mean age was 58±17.49y.Baseline total MGS scores in both treatment groups were comparable.During follow-up,there were significant differences in total MGS and per sector with P<0.001.Multivariate analysis was performed using generalized estimating equations corresponding to the generalized linear model for repeated means to determine the treatment relationship with total MGS,NIBUT,and OSDI.There was a significant difference between ILux®and MMGE(P<0.001)at follow-up from the first to the twelfth month in MGS,NI-BUT,and OSDI scores.No adverse events were reported.CONCLUSION:ILux®treatment compared to MMGE significantly improves symptoms and signs in patients with moderate to severe MGD for one year without adverse events.

关键词： meibomian gland dysfunction ILux® ocular surface dry eye

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Does the provision of live black soldier fly and yellow mealworm larvae improve Muscovy duck welfare?

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Journal of Animal Science and Biotechnology 2024年第1期15卷 442-455页

作者： Marta Gariglio Sihem Dabbou Manuela Renna Ilaria Biasato Sara Bellezza Oddon Marco Meneguz raul Daniel Miazzo Stefania Bergagna Elena Colombino Elisabetta Macchi Achille Schiavone Department of Veterinary Sciences University of TurinLargo Paolo Braccini 2GrugliascoTorino(TO)10095Italy Center Agriculture Food Environment(C3A) University of TrentoVia Edmund Mach 1San Michele All’AdigeTrento(TN)38098Italy Department of Agricultural Forest and Food SciencesUniversity of TurinLargo Paolo Braccini 2GrugliascoTorino(TO)10095Italy BEF Biosystems s.r.l Via Tancredi Canonico 18/CTorino(TO)10156Italy Unidad de Investigacion Aviar Produccion AvicolaFac.Agr.y Vet.Universidad Nacional de Rio CuartoCordobaArgentina Veterinary Medical Research Institute for Piemonte Liguria and Valle d’AostaVia Bologna 148Torino(TO)10154Italy

Background The provision of environmental enrichments to Muscovy ducks could reduce the expression of the aggressive behaviors.The aim of the present study was to evaluate the effects of black soldier fly(BSF)and yellow mealworm(YM)live larva provision on Muscovy duck performance,excreta corticosterone metabolites(ECM),behavior,and blood parameters.Methods A total of 1263-day-old female Muscovy ducklings were allotted to 18 pens(6 replicates/treatment,7 birds/pen)and assigned to 3 experimental treatments:a control group fed commercial feed,and two experimental treatments fed commercial feed plus the 5%(based on the expected daily feed intake,as fed basis)of BSF and YM live larvae(BSF and YM groups,respectively).A two-phase feeding program was applied:starter(from 3 to 31 days of age)and grower-finisher(from 32 to 55 days of age).The live weight,average daily gain,average daily feed intake,and feed conversion ratio were calculated.Larva consumption times were collected,and video recordings were performed during 3 periods(P)each day:the hour before(P1),during(P2),and after(P3)the larva administration.ECM were evaluated at 3,31,and 55-day-old.Finally,the total red and white blood cell counts,serum proteins,lipids,and liver and renal function serum enzymes were evaluated on 12 birds/treatment.Results The experimental treatment did not affect the growth performance of the birds(P>0.05).Larva consumption times were always similar between the two insect species,except at 14–18 days of age,were BSF larvae were consumed faster than YM larvae(PP3;P<0.05).Finally,the provision of live BSF and YM larvae significantly reduced the ECM at 55 days of age and the heterophil to lymphocyte ratio(P<0.05).Conclusions Live BSF and YM larva supplementation in Muscovy duck improves duck welfare,without

关键词： Behavior Cairina moschata Environmental enrichment Hermetia illucens Poultry Tenebrio molitor Welfare

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Outcomes and efficacy of magnetic resonance imaging-compatible sacral nerve stimulator for management of fecal incontinence: A multi-institutional study

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World Journal of Radiology 2024年第2期16卷 32-39页

作者： Binit Katuwal Amy Thorsen Kunal Kochar Ryba Bhullar Ray King Ernesto raul Drelichman Vijay K Mittal Jasneet Singh Bhullar Department of Surgery Ascension Providence HospitalMichigan State University College of Human MedicineSouthfieldMI 48075United States Department of Colon and Rectal surgery Alina Health Abbott Northwestern HospitalMinneapolisMN 55407United States Colon and Rectal Surgery Advocate Illinois MasonicPark RidgeIL 60068United States Volunteer Student Department of ResearchAscension Providence HospitalSouthfieldMI 48075United States Colon and Rectal Surgery University of WisconsinMadisonWI 53715United States General and Colorectal Surgery Ascension Providence HospitalMichigan State University College of Human MedicineSouthfieldMI 48075United States Department of Surgery Department of Medical EducationAscension Providence HospitalMichigan State University College of Human MedicineSouthfieldMI 48075United States

BACKGROUND Fecal incontinence(FI)is an involuntary passage of fecal matter which can have a significant impact on a patient’s quality of life.Many modalities of treatment exist for FI.Sacral nerve stimulation is a well-established treatment for FI.Given the increased need of magnetic resonance imaging(MRI)for diagnostics,the In-terStim which was previously used in sacral nerve stimulation was limited by MRI incompatibility.Medtronic MRI-compatible InterStim was approved by the United States Food and Drug Administration in August 2020 and has been widely used.AIM To evaluate the efficacy,outcomes and complications of the MRI-compatible InterStim.METHODS Data of patients who underwent MRI-compatible Medtronic InterStim placement at UPMC Williamsport,University of Minnesota,Advocate Lutheran General Hospital,and University of Wisconsin-Madison was pooled and analyzed.Patient demographics,clinical features,surgical techniques,complications,and outcomes were analyzed.Strengthening the Reporting of Observational studies in Epidemiology(STROBE)cross-sectional reporting guidelines were used.RESULTS Seventy-three patients had the InterStim implanted.The mean age was 63.29±12.2 years.Fifty-seven(78.1%)patients were females and forty-two(57.5%)patients had diabetes.In addition to incontinence,overlapping symptoms included diarrhea(23.3%),fecal urgency(58.9%),and urinary incontinence(28.8%).Fifteen(20.5%)patients underwent Peripheral Nerve Evaluation before proceeding to definite implant placement.Thirty-two(43.8%)patients underwent rechargeable InterStim placement.Three(4.1%)patients needed removal of the implant.Migration of the external lead connection was observed in 7(9.6%)patients after the stage I procedure.The explanation for one patient was due to infection.Seven(9.6%)patients had other complications like nerve pain,hematoma,infection,lead fracture,and bleeding.The mean follow-up was 6.62±3.5 mo.Sixty-eight(93.2%)patients reported significant improvement of symptoms on follow-up evaluation.CONCLUSION This study show

关键词： Fecal incontinence Sacral nerve stimulation InterStim Magnetic resonance imaging Sacral neuromodulation

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Association between Helicobacter pylori infection,mismatch repair,HER2 and tumor-infiltrating lymphocytes in gastric cancer

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World Journal of Gastrointestinal Oncology 2024年第6期16卷 2487-2503页

作者： Carlos A Castaneda Miluska Castillo Luis A Bernabe Joselyn Sanchez Matteo Fassan Katherine Tello Ignacio Ivan Wistuba Ivan Chavez Passiuri Eloy Ruiz Juvenal Sanchez Fernando Barreda Daniel Valdivia Yaqueline Bazan Milagros Abad-Licham Claudio Mengoa Hugo Fuentes Paola Montenegro Ebert Poquioma raul Alatrista Claudio J Flores Luis Taxa Faculty of Health Sciences Universidad Cientifica del SurLima 15038Peru GECO PERU Grupo de Estudios Clinicos Oncologicos del PeruLima 15038Peru Department of Research Instituto Nacional de Enfermedades NeoplasicasLima 15038Peru Faculty of Human Medicine Universidad Ricardo PalmaLima 15039Peru Department of Medicine Surgical Pathology&Cytopathology UnitUniversity of PaduaPadua 35121Italy Department of Translational Molecular Pathology The University of Texas MD Anderson Cancer CenterHoustonTX 77030United States Department of Abdominal Surgery Instituto Nacional de Enfermedades NeoplasicasLima 15038Peru Department of Pathology Instituto Nacional de Enfermedades NeoplasicasLima 15038Peru Department of Medical Specialties Instituto Nacional de Enfermedades NeoplasicasLima 15038Peru Department of Pathology Instituto Regional de Enfermedades Neoplasicas del NorteTrujillo 13001Peru Faculty of Human Medicine Universidad Privada Antenor OrregoTrujillo 13008Peru Department of Surgery Instituto Regional de Enfermedades Neoplasicas del SurArequipa 04002Peru Department of Medical Oncology Instituto Nacional de Enfermedades NeoplasicasLima 15038Peru Department of Epidemiology Instituto Nacional de Enfermedades NeoplasicasLima 15038Peru Unidad de Investigación Básica y Traslacional Oncosalud-AUNALima 15038Peru Faculty of Medicine Universidad San Martin de PorresLima 15008Peru

BACKGROUND The influence of Helicobacter-pylori(H.pylori)infection and the characteristics of gastric cancer(GC)on tumor-infiltrating lymphocyte(TIL)levels has not been extensively studied.Analysis of infiltrating-immune-cell subtypes as well as survival is necessary to obtain comprehensive information.AIM To determine the rates of deficient mismatch-repair(dMMR),HER2-status and H.pylori infection and their association with TIL levels in GC.METHODS Samples from 503 resected GC tumors were included and TIL levels were evaluated following the international-TILs-working-group recommendations with assessment of the intratumoral(IT),stromal(ST)and invasive-border(IB)compartments.The density of CD3,CD8 and CD163 immune cells,and dMMR and HER2-status were determined by immunohistochemistry(IHC).H.pylori infection was evaluated by routine histology and quantitative PCR(qPCR)in a subset of samples.RESULTS dMMR was found in 34.4%,HER2+in 5%and H.pylori-positive in 55.7%of samples.High IT-TIL was associated with grade-3(P=0.038),while ST-TIL with grade-1(P<0.001),intestinal-histology(P<0.001)and no-recurrence(P=0.003).dMMR was associated with high TIL levels in the ST(P=0.019)and IB(P=0.01)compartments,and STCD3(P=0.049)and ST-CD8(P=0.05)densities.HER2-was associated with high IT-CD8(P=0.009).H.pylorinegative was associated with high IT-TIL levels(P=0.009)when assessed by routine-histology,and with high TIL levels in the 3 compartments(P=0.002-0.047)and CD8 density in the IT and ST compartments(P=0.001)when assessed by qPCR.A longer overall survival was associated with low IT-CD163(P=0.003)and CD8/CD3(P=0.001 in IT and P=0.002 in ST)and high IT-CD3(P=0.021),ST-CD3(P=0.003)and CD3/CD163(P=0.002).CONCLUSION TIL levels were related to dMMR and H.pylori-negativity.Low CD8/CD3 and high CD163/CD3 were associated with lower recurrence and longer survival.

关键词： Lymphocytes Macrophages Gastric cancer Helicobacter pylori HER2 Mismatch repair

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Nodal involvement and p16-staining in upper alveolar ridge and hard palate cancer

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Journal of Cancer Metastasis and Treatment 2018年第1期4卷 186-198页

作者： Edgar Salas Pedro Sanchez Juan Postigo Carlos A.Castaneda Miluska Castillo Valeria Villegas Luis Cano Sandro Casavilca Luis A.Bernabe Carolina Belmar Maria R.Villa-Robles raul mantilla Henry Guerra Head&Neck Surgery Department Instituto Nacional de Enfermedades NeoplasicasLima 15038Peru Research Department Instituto Nacional de Enfermedades NeoplasicasLima 15038Peru Faculty of Medicine Universidad Peruana San Juan BautistaLima 15067Peru Pathology Department Instituto Nacional de Enfermedades NeoplasicasLima 15038Peru

Aim:Upper alveolar ridge and hard palate squamous cancer is an infrequent malignancy.We evaluated factors associated with neck involvement and with p16-staining.Methods:Head and neck squamous-cell carcinoma(SCC)patients who went to Head and Neck Department between 1997 and 2011 were screened,and 73 resected upper alveolar ridge and 5 hard palate SCC were selected.Tumors with available tissue were stained with p16 immunohistochemistry.Results:Median age was 64.4 years,55.1%were female,and 73.1%were in clinical stage IV.Neck dissections were performed in 24 and pathologically confirmed node metastases were found in 19(24.3%).Cervical recurrence was found in 18 patients(23.1%)and was associated with histological grade(P=0.037).Three(7.3%)of 41 lesions were positive for p16 and tended to be younger(P=0.067).Lymphovascular invasion was associated with shorter disease-free survival(DFS)(P=0.026)and overall survival(OS)(P=0.021).Larger cT(P=0.019),perineural invasion(P=0.039)and neck dissection(P=0.010)were associated with shorter OS.Neck node involvement tended to have shorter DFS(31%vs.48.7%,P=0.278)and OS(25.1%vs.48.5%,P=0.340),and neck recurrence tended to have shorter OS(9.3%vs.52.3%,P=0.064).Conclusion:Neck involvement and recurrence are frequent in this location.P16-positive cases were present in 7.3%and tended to be associated with younger age.

关键词： P16 head neck maxilla prognosis survival

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Development and screening of(Ca-P-Si-F)-PEO coatings for biodegradability control of Mg-Zn-Ca alloys

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Journal of Magnesium and Alloys 2022年第8期10卷 2220-2237页

作者： Lara Moreno Marta Mohedano raul Arrabal Endzhe Matykina Departamento de Ingeniería Química y de Materiales Facultad de Ciencias QuímicasUniversidad Complutense de Madrid28040 MadridSpain

PEO coatings were developed on MgZnxCayalloys for biomedical applications using different treatment times and a novel transparent electrolyte containing Ca,P,Si and F.Surface characteristics and chemical composition of studied materials were evaluated using scanning electron microscopy,X-ray diffraction and optical metrology.Corrosion investigations were performed for uncoated and coated materials using potentiodynamic polarization,electrochemical impedance spectroscopy and hydrogen evolution measurements in three media(0.9wt.%NaCl,α-MEM and inorganic part ofα-MEM).Findings revealed that Zn-rich intermetallics favour the formation of large voids in the coatings.Ca,P,F and Si are successfully incorporated into the coatings.Coatings consist of crystalline phases(Mg O,Mg F_(2)and Ca,Si-rich compounds)and amorphous material.Aminoacids and organic components ofα-MEM solution accelerate corrosion of the bare substrates but have no significant influence on the corrosion resistance of coated specimens.Therefore,inorganicα-MEM can be considered as an appropriate medium for fast screening of PEO coatings.The relatively small amount of F content in the barrier layer facilitates early failure of the coatings,which show undercoating corrosion.Zn-rich intermetallic particles in the undercoating crevice induce a more aggressive corrosive environment and enhanced micro-galvanic couple effect with the Mg matrix.This leads to faster degradation of the coated alloys compared to the bare substrates.

关键词： Magnesium Biomaterials Plasma electrolytic oxidation Corrosion

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A numerical case study on the long-term seismic assessment of reinforced concrete tunnels in corrosive environments

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Journal of Rock Mechanics and Geotechnical Engineering 2023年第3期15卷 551-572页

作者： Maria Antoniou Antonios Mantakas Nikolaos Nikitas raul Fuentes ETH Zurich ZurichSwitzerland National Technical University of Athens AthensGreece University of Leeds LeedsUK RWTH Aachen University AachenGermany

The paper investigates the long-term seismic behaviour of an underground reinforced concrete(RC)metro tunnel in Santiago,Chile,considering the combined effects of chloride-induced corrosion and cumulative,low-amplitude seismic shaking on the structure’s performance.The soil-tunnel response is evaluated with the aid of transient,nonlinear finite element analysis using a two-dimensional(2D)plane strain numerical model that adopts advanced nonlinear models for the simulation of soil and concrete plasticity and the dynamic stiffness behaviour.The effects of corrosion deterioration are demonstrated in terms of time-dependent loss of rebar area and cover concrete stiffness and strength.The study illustrates the influence of ageing and repeated seismic shaking on lining deformation,crack development,and the modal characteristics of the intact and degrading systems.The results indicate that multiple lowamplitude events drive the non-degrading RC tunnel beyond its elastic regime without significant structural response consequences.A noticeable impact of corrosion deterioration on the structure’s seismic performance is revealed,increasing with the number and intensity of earthquake events.Two different tunnel embedment depths are comparatively assessed.The analyses demonstrate larger coseismic section convergence in the case of the deeper tunnel,yet a less pronounced effect of ageing and successive seismic loading compared to the shallow section,which is evident in the RC lining cracks at the end of shaking.

关键词： Tunnels Reinforcement corrosion Ageing Earthquakes Numerical modelling Long-term performance Concrete cracking behaviour

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Tree ecophysiology in the context of climate change

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Journal of Forestry Research 2023年第1期34卷 1-5页

作者： Yanbo Hu raul Antonio Sperotto Georgios Koubouris Srđan Stojnić Nacer Bellaloui Northeast Forestry University Harbin 150040People’s Republic of China Graduate Program in Biotechnology Life Sciences AreaUniversity of Taquari Valley-UnivatesLajeadoBrazil Graduate Program in Plant Physiology Federal University of PelotasPelotasBrazil Laboratory of Olive Cultivation Institute of Olive TreeSubtropical Crops and ViticultureHellenic Agricultural Organization DIMITRALeoforos Karamanli 16773134 ChaniaCreteGreece Institute of Lowland Forestry and Environment University of Novi SadAntonaČehova 13dNovi SadSerbia Crop Genetics Research Unit Agricultural Research ServiceUSDA141 Experiment Station RoadStonevilleMS 38776USA

Forest structure and function strongly depend on and concurrently influence environmental conditions.Tree performance is generally governed by its genetics and environment;thus,recent hotspots in this field include tree genotype×environment,phenotype×environment,and functional trait×environment interactions.The editorial,review,and 22 original research articles in this Special Issue,"Tree ecophysiology in the context of climate change",highlight ecophysiological phenomena(e.g.,climate hormesis,seed germination,tree mortality),processes(e.g.,tree metabolism,photosynthate allocation,nutrient uptake and transport),indicators(e.g.,carbon sequestration,pollutants),measurements(e.g.,thermal time methods,soil quality indices,vegetation spectral index,and near-infrared leaf reflectance),and modeling(e.g.,climate correlations with tree growth,photo synthetic phenology,hydraulic strategies,OliveCan model)in the context of global climate change.Understanding forest-environment interactions from an ecophysiological perspective as climate changes provides insights into species fitness in suboptimal environments,species competition for limited resources,and phylogenetic divergence or convergence of species,and predicting species distributions.

关键词： Tree ecophysiology Climate change Climate hormesis Phenology Thermostability Forest resilience Hydraulics

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As-cast and extruded Mg-Zn-Ca systems for biodegradable implants:Characterization and corrosion behavior

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Journal of Magnesium and Alloys 2023年第3期11卷 1102-1120页

作者： Lara Moreno Endzhe Matykina Kiryl A.Yasakau Carsten Blawert raul Arrabal Marta Mohedano Departamento de Ingeniería Química y de Materiales Facultad de Ciencias QuímicasUniversidad Complutense de Madrid28040 MadridSpain Department of Materials and Ceramic Engineering CICECO-Aveiro institute of materialsUniversity of Aveiro3810-193 AveiroPortugal Institute of Surface Science Helmholtz-Zentrum HereonMax-Planck Strasse 1Geesthacht 21502Germany

The aim of the present study is to evaluate the effect of alloy processing and composition as well as the pH control and testing medium on the in vitro corrosion performance of Mg-Zn-Ca systems for biodegradable implants.The grain size and secondary phases were analyzed by optical microscopy,scanning electron microscopy,transmission electron microscopy,and X-ray diffraction.Scanning kelvin probe force microscopy(SKPFM)was used to analyze the Volta potential values of the second phases.The corrosion performance of the three alloys was evaluated by electrochemical and hydrogen evolution methods inα-MEM with and without organic species(i.***.complete and inorganicα-MEM).Two strategies were followed to evaluate the influence of the pH on the corrosion behavior:daily solution replacement and CO_(2)flow based pH control.For all the materials,the organic medium accelerates the corrosion process.Constant pH maintained by CO_(2)flow through the medium results in considerably higher corrosion rates for all alloys.The impact of pH is lesser on the as-cast alloys due to the barrier effect of the secondary phases,particularly pronounced in the Mg1Zn1Ca alloy which showed the lowest corrosion rate.The wrought Mg0.5Zn0.2Ca alloy that lacks the refined secondary phase network and exhibits high number of twins undergoes accelerated uniform corrosion under constant pH conditions.

关键词： MgZnCa Magnesium Biomaterials Characterization Corrosion

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Designing nanocarriers to overcome the limitations in conventional drug administration for Parkinson’s disease

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Neural Regeneration Research 2022年第8期17卷 1743-1744页

作者： Rafael A.García-Muñoz Joseph McConnell Victoria Morales raul Sanz Department of Chemical and Environmental Technology Rey Juan Carlos UniversityMadridSpain Department of Chemical&Process Engineering University of StrathclydeGlasgowUK

Neurodegenerative diseases(NDs)have become one of the leading causes of death and disability worldwide,and cause enormous pain and suffering for both patients and their families.Some of the most common NDs include Alzheimer’s disease,Parkinson’s disease(PD)and Huntington’s disease,among others(Feng,2020).PD is a widespread neurodegenerative disease that affects more than 10 million people worldwide(No author listed,2021).The direct cause of the disease is unknown,but it is characterized by the selective degeneration of dopaminergic neurons in the midbrain in the substantia nigra.This leads to the depletion of dopamine(3,4-dihydroxyphenethylamine,DA)in the striatum of patients,in addition to the existence of abnormalα-synuclein in nerve cells and the development of toxic protein aggregates in neurons called Lewy bodies,which causes muscle stiffness,slowness of movements and tremors.It is believed that a combination of genetic and environmental factors may be the cause of PD,but the exact reason for the disease is not yet fully understood.

关键词： Huntington degeneration overcome

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