Association between Helicobacter pylori infection,mismatch repair,HER2 and tumor-infiltrating lymphocytes in gastric cancer
作者机构：Faculty of Health SciencesUniversidad Cientifica del SurLima 15038Peru GECO PERUGrupo de Estudios Clinicos Oncologicos del PeruLima 15038Peru Department of ResearchInstituto Nacional de Enfermedades NeoplasicasLima 15038Peru Faculty of Human MedicineUniversidad Ricardo PalmaLima 15039Peru Department of MedicineSurgical Pathology&Cytopathology UnitUniversity of PaduaPadua 35121Italy Department of Translational Molecular PathologyThe University of Texas MD Anderson Cancer CenterHoustonTX 77030United States Department of Abdominal SurgeryInstituto Nacional de Enfermedades NeoplasicasLima 15038Peru Department of PathologyInstituto Nacional de Enfermedades NeoplasicasLima 15038Peru Department of Medical SpecialtiesInstituto Nacional de Enfermedades NeoplasicasLima 15038Peru Department of PathologyInstituto Regional de Enfermedades Neoplasicas del NorteTrujillo 13001Peru Faculty of Human MedicineUniversidad Privada Antenor OrregoTrujillo 13008Peru Department of SurgeryInstituto Regional de Enfermedades Neoplasicas del SurArequipa 04002Peru Department of Medical OncologyInstituto Nacional de Enfermedades NeoplasicasLima 15038Peru Department of EpidemiologyInstituto Nacional de Enfermedades NeoplasicasLima 15038Peru Unidad de Investigación Básica y TraslacionalOncosalud-AUNALima 15038Peru Faculty of MedicineUniversidad San Martin de PorresLima 15008Peru
出 版 物：《World Journal of Gastrointestinal Oncology》 (世界胃肠肿瘤学杂志（英文版）（电子版）)
年 卷 期：2024年第16卷第6期
页 面：2487-2503页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：Supported by Ministerio de la Produccion de Peru,No.317-PNICP-EC-2014,and No.430-PNICP-PIAP-2014 Consejo Nacional de Ciencia Tecnologia e Innovacion Tecnologica,No.196-2015-FONDECYT,No.197-2015-FONDECYT,and No.204-2015-FONDECYT.
主 题：Lymphocytes Macrophages Gastric cancer Helicobacter pylori HER2 Mismatch repair
摘 要：BACKGROUND The influence of Helicobacter-pylori(H.pylori)infection and the characteristics of gastric cancer(GC)on tumor-infiltrating lymphocyte(TIL)levels has not been extensively studied.Analysis of infiltrating-immune-cell subtypes as well as survival is necessary to obtain comprehensive information.AIM To determine the rates of deficient mismatch-repair(dMMR),HER2-status and H.pylori infection and their association with TIL levels in GC.METHODS Samples from 503 resected GC tumors were included and TIL levels were evaluated following the international-TILs-working-group recommendations with assessment of the intratumoral(IT),stromal(ST)and invasive-border(IB)compartments.The density of CD3,CD8 and CD163 immune cells,and dMMR and HER2-status were determined by immunohistochemistry(IHC).H.pylori infection was evaluated by routine histology and quantitative PCR(qPCR)in a subset of samples.RESULTS dMMR was found in 34.4%,HER2+in 5%and H.pylori-positive in 55.7%of samples.High IT-TIL was associated with grade-3(P=0.038),while ST-TIL with grade-1(P0.001),intestinal-histology(P0.001)and no-recurrence(P=0.003).dMMR was associated with high TIL levels in the ST(P=0.019)and IB(P=0.01)compartments,and STCD3(P=0.049)and ST-CD8(P=0.05)densities.HER2-was associated with high IT-CD8(P=0.009).H.pylorinegative was associated with high IT-TIL levels(P=0.009)when assessed by routine-histology,and with high TIL levels in the 3 compartments(P=0.002-0.047)and CD8 density in the IT and ST compartments(P=0.001)when assessed by qPCR.A longer overall survival was associated with low IT-CD163(P=0.003)and CD8/CD3(P=0.001 in IT and P=0.002 in ST)and high IT-CD3(P=0.021),ST-CD3(P=0.003)and CD3/CD163(P=0.002).CONCLUSION TIL levels were related to dMMR and H.pylori-negativity.Low CD8/CD3 and high CD163/CD3 were associated with lower recurrence and longer survival.
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