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Journal of Bionic Engineering 2021年第1期18卷 103-117页

作者： Shailendra Singh Shera rathindra mohan banik Bioprocess Technology Laboratory Sschool of Biochemical Engineeringndian Institute of Technology(Banaras Hindu University)Varanasi 221005India Department of Biotechnology Faculty of Engineering&TechnologyRAMA Universit Uittar PradeshKanpur 209217India

Skin acts as protective barrier against a number of factors such as dust,opportunistic microbial and viral infections,regulates body temperature and waste *** cell population plays an important role in devclopment of skin architecturc.A scaffold having capability to support and enhance fibroblast growth is a viable option for wound dressing material which can shorten the time for wound to *** this work,Silk Fibroin(SF)and Xanthan(Xa)were blended in three ratios 80 SF:20 Xa(SFX82),60 SF:40 Xa(SFX64),and 50SF:50 Xa(SFX55)to create SF/Xa *** and other physicochemical properties of SF/Xa scaffold are functions of blending ratios and blend with the ratio 80 SF:20 Xa has the highest *** properties of SF/Xa blends are a function of miscibility with SFX82 having superior thermal propertis of all fabricated *** porosity of SF/Xa scaffolds is in the range of 67%to 50%,with pore size of 58.1 um-45.5 um,water uptake capacity of 92%-86%,and surface roughness of 49.95 nm-385 ***82 shows highest growth rate of L929 fibroblast cells indicating its superiority over other scaffolds for providing biological cues for the growth and proliferation of fibroblastic cells in natural ***82 scaffold is found to be most suitable for fibroblastic cells thereby enhancing the tissue regeneration at wound site.

关键词： blends miscibility FTIR imaging Atomic Force Microscopy(AFM) cytocompatibility wound healing

Invitro Biodegradability of Silk Fibroin/Xanthan Biopolymeric Composite Scaffolds

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Journal of Bionic Engineering 2019年第2期16卷 299-310页

作者： Shailendra Singh Shera Shraddha Sahu rathindra mohan banik Bioprocess Technology Laboratory School of Biochemical Engineering Indian Institute of TechnologyBanaras Hindu UniversityVaranasi 221005India

Silk fibroin/xanthan scaffolds were prepared by blending silk fibroin and xanthan in the ratios 80SF:20Xa(SFX82),60SF:40Xa(SFX64),and 50SF:50Xa(SFX55)using freeze drying ***-vitro degradation behavior of the prepared scaffolds was studied for 37 days in phosphate buffer *** degradation rate was the function of silk fibroin,xanthan and^-crystallite contents in the silk fibroin/xanthan ***82 degraded extremely slowly whereas SFX55 showed faster degradation *** xanthan was the main contributor of weight ***82 and SFX64 exhibited surface degradation whereas SFX55 showed bulk degradation which indicated that higher silk fibroin ratios favor surface *** to bulk degradation,SFX55 showed maximum surface roughness among the composite *** FTIR spectrum revealed total loss of xanthan from the composites after *** broad and low-intensity peaks in the FTIR spectrum of composite scaffolds con finned reduction in 0-sheet crystallite content during *** analysis also confirmed reduction in 0-sheet crystals and revealed that degraded composite scaffold had predominantly amorphous *** degraded scaffold showed higher porous structure than the non-degraded *** in vitro degradability testing gives a good approximation of degradation of scaffold in vivo and helps in designing a robust biopolymeric composite scaffold for tissue engineering.

关键词： biopolymer composites surface roughness biopolymer degradation surface degradation tissue engineering

Artificial Neural Network Modeling to Predict the Non-Linearity in Reaction Conditions of Cholesterol Oxidase from <i>Streptomyces olivaceus</i><i>MTCC</i>6820

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Journal of Biosciences and Medicines 2019年第4期7卷 14-24页

作者： Shraddha Sahu Shailendra Singh Shera rathindra mohan banik Bioprocess Technology Laboratory School of Biochemical Engineering Indian Institute of Technology (Banaras Hindu University) Varanasi India

Cholesterol oxidase (COX) is widely used enzyme for total cholesterol estimation in human serum and for the fabrication of electro-chemical biosensors. COX is also used for the bioconversion of cholesterol;for the production of precursors of steroidal drugs and hormones. Enzyme activity depends decisively on defined conditions with respect to pH, temperature, ionic strength of the buffer, substrate concentration, enzyme concentration, reaction time. Standardization of these parameters is desirable to attain optimum activity of the enzyme. The present work aims to build a neural network model using five input parameters (pH, cholesterol concentration, 4-aminoantipyrine concentration, crude COX volume and horseradish peroxidase) and one output i.e., COX activity (U/ml) as a response. A feed forward back propagation neural network with Levenberg-Marquardt training algorithm was used to train the network. The network performance was assessed in terms of regression (R2), Mean Squared Error (MSE) and Mean Absolute Percentage Error (MAPE). A network topology of 5-10-1 was found to be optimum. The MSE, MAPE and R2 values of the neural model were 0.0075%, 0.12% and 0.9792% respectively. The maximum predicted activity of COX was 1.073 U/ml, which was close to the experimental value i.e., 1.1 U/ml at simulated optimum assay conditions. MSE and MAPE depicted the precision in the prediction efficiency of the developed ANN model. Higher R2 value showed a good correlation between the experimental and ANN predicted values. This proved the robustness of the ANN model to predict similar type of system (COX from other Streptomyces sp.) within the limits of the trained data set. The COX activity was enhanced by 1.71 folds after optimization of the reaction conditions.

关键词： Cholesterol Oxidase Artificial Neural Network Optimization Streptomyces olivaceus Prediction

Identification of circulating CD90^+ CD73^+ cells in cirrhosis of liver

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World Journal of Stem Cells 2011年第7期3卷 63-69页

作者： Mitnala Sasikala Pugazhelthi Surya Gaddipati Radhika Pondugala Pavan Kumar Mekala Subba Rao rathindra mohan Mukherjee Padaki Nagaraja Rao D Nageshwar Reddy Institute of Basic Sciences and TranslationalResearch Asian Health Care Foundation Asian Institute of Gastroenterology

AIM:To identify circulating CD90 + CD73 + CD45 cells and evaluate their in vitro proliferating ***:Patients with cirrhosis(n=43),and healthy volunteers(n=40)were recruited to the *** cells were isolated and cultured from the peripheral blood of controls and cirrhosis ***-like cells that appeared in cultures were analyzed for morphological features,enumerated by flow cytometry and confirmed by immunocytochemistry(ICC).Colony forming efficiency(CFE)of these cells was assessed and expressed as a ***:In comparison to healthy volunteers,cells obtained from cirrhotic patients showed a significantincrease(P<0.001)in the percentage of CD90+CD73+ CD45 cells in *** cells also showed 10 fold increases in *** cytometry and ICC confirmed that the proliferating cells expressed CD90 + CD73 + in the cultures from cirrhosis ***:These results indicate the presence of circulating CD90 + CD73 + CD45 cells in patients with liver cirrhosis that have the potential to proliferate at a higher rate.

关键词： CD90+ CD73+ CD45 cells Liver cirrhosis Proliferation Colony forming efficiency