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Molecular and in vitro biochemical assessment of chemosensory protein 10 from brown planthopper Nilaparvata lugens at acidic pH

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Journal of Integrative Agriculture 2022年第3期21卷 781-796页

作者： muhammad Irfan WARIS Aneela YOUNAS rana muhammad kaleem ullah Fatima RASOOL muhammad Muzammal ADEEL WANG Man-qun College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070P.R.China National Center for Bioinformatics Quaid-i-Azam UniversityIslamaba 45320Pakistan College of Informatics Huazhong Agricultural UniversityWuhan 430070P.R.China

Chemosensory proteins(CSPs)are important molecular components of the insect olfactory system,which are involved in capturing,binding,and transporting hydrophobic odour molecules across the sensillum in sensillar lymph in regulating insect *** protein family(CSPs)is also involved in many other systems that are not linked to olfactory receptors in olfactory *** brown planthopper(BPH)is a monophagous pest of rice that causes damage by sucking phloem sap and transmitting a number of diseases caused by *** this study,fluorescence competitive binding assay and fluorescence quenching assay at acidic p H were performed as well as homology modelling to describe the binding affinity of Nlug *** competitive binding assay(FCBA)demonstrated that Nlug CSP10 bound strongly to nonadecane,farnesene,and 2-tridecanone at acidic p *** results of FCBA indicated that Nlug CSP10 bound different ligands at the physiological p H(5.0)of the bulk sensillum *** quenching assay demonstrated that Nlug CSP10 generated a stable complex with 2-tridecanone,while two ligands nonadecane and farnesene collided due to molecular *** interaction of selected ligands with the modelled structure of Nlug CSP10 was also analyzed,which found the key amino acids(Gln23,Gln24,Gln25,Asn27,Met33,Ser34,Ile35,Tyr36,Asn42,Met43,Val45,Asn46,Asn93,Arg96,Ala97,Lys99,and Ala100)in Nlug CSP10 that were involved in binding of volatile *** present study contributes to the binding profile of Nlug CSP10 that promotes the development of behaviorally active ligands based on BPH olfactory system.

关键词： insect olfaction chemosensory protein Nilaparvata lugens fluorescence competitive binding assay fluorescence quenching assay molecular docking

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The role of chemosensory protein 10 in the detection of behaviorally active compounds in brown planthopper, Nilaparvata lugens

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Insect Science 2020年第3期27卷 531-544页

作者： muhammad Irfan Waris Aneela Younas muhammad Muzammal Adeel Shuang-Gang Duan Sundas rana Quershi rana muhammad kaleem ullah Man-Qun Wang Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina College of informatics Huazhong Agricultural UniversityWuhanChina

Chemosensory proteins(CSPs)play important roles in insects’chemoreception,although their specific functional roles have not been fully *** this study,we conducted the developmental expression patterns and competitive binding assay as well as knock‐down assay by RNA interference both in vitro and in vivo to reveal the function of NlugCSP10 from the brown planthopper(BPH),Nilaparvata lugens(Stål),a major pest in rice *** results showed that NlugCSP10 messenger RNA was significantly higher in males than in females and correlated to gender,development and wing *** fluorescence binding assays revealed that NlugCSP10 exhibited the highest binding affinity with cis‐3‐hexenyl acetate,eicosane,and(+)‐β‐*** assay revealed that eicosane displayed attractant activity,while cis‐3‐hexenyl acetate,similar to(+)‐β‐pinene significantly repelled *** *** of NlugCSP10,which is responsible for cis‐3‐hexenyl acetate binding,significantly disrupted cis‐3‐hexenyl acetate ***,findings of the present study showed that NlugCSP10 could selectively interrelate with numerous volatiles emitted from host plants and these ligands could be designated to develop slow‐release mediators that attract/repel *** and subsequently improve the exploration of plans to control this insect pest.

关键词： behavioral responses chemosensory proteins expression patterns fluorescence competitive binding assays RNA interference

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中巴经济走廊是巴基斯坦的中国债务陷阱吗？

中巴经济走廊是巴基斯坦的中国债务陷阱吗？

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作者： kaleem ullah KHAN 山东财经大学

学位级别：硕士

中巴经济走廊(CPEC)是中国“一带一路”倡议(BRI)下的旗舰项目,引发了对其经济影响和对巴基斯坦债务可持续性潜在影响的广泛讨论。本研究对中巴经济走廊(CPEC)进行了全面分析,探讨了其多方面的维度,包括基础设施发展、能源项目、经济增... 详细信息

中巴经济走廊(CPEC)是中国“一带一路”倡议(BRI)下的旗舰项目,引发了对其经济影响和对巴基斯坦债务可持续性潜在影响的广泛讨论。本研究对中巴经济走廊(CPEC)进行了全面分析,探讨了其多方面的维度,包括基础设施发展、能源项目、经济增长潜力和地缘政治意义。通过对经验证据、金融机制??和项目成果的审查,该研究批判性地评估了中巴经济走廊(CPEC)作为巴基斯坦潜在“债务陷阱”的普遍说法,为该走廊对巴基斯坦经济的影响提供了细致入微的视角。调查结果显示,中巴经济走廊是巴基斯坦经济转型、加强能源安全、基础设施发展和区域互联互通的重要催化剂。中巴经济走廊(CPEC)下的战略投资具有促进长期经济增长、提高出口竞争力并吸引大量外国直接投资的潜力。然而,这些好处的实现取决于有效的债务管理、经济政策改革和资金来源的多元化。该研究强调了透明财务规划和实施全面改革的重要性,以确保中巴经济走廊的经济红利有助于可持续发展和债务可持续性。与简化的“债务陷阱”叙述相反,本研究认为,通过战略性金融管理和结构性改革,中巴经济走廊可以在增强巴基斯坦经济主权和促进可持续发展方面发挥关键作用。该研究的结论是,尽管面临挑战,中巴经济走廊仍为经济振兴和区域一体化提供了前所未有的机遇,标志着巴中关系进入了新时代,并为“一带一路”的更广泛目标做出了贡献。

关键词：中巴经济走廊(CPEC) 一带一路倡议(BRI) 债务可持续性

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Deep Learning and Machine Learning-Based Model for Conversational Sentiment Classification

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Computers, Materials & Continua 2022年第8期72卷 2323-2339页

作者： Sami ullah muhammad Ramzan Talib Toqir A.rana muhammad Kashif Hanif muhammad Awais Department of Computer Science Government College University Faisalabad38000Pakistan Department of Computer Science and IT The University of Lahore54590Pakistan School of Computer Sciences Universiti Sains Malaysia(USM)11800PenangMalaysia Department of Software Engineering Government College University Faisalabad38000Pakistan

In the current era of the internet,people use online media for conversation,discussion,chatting,and other similar *** of such material where more than one person is involved has a spate challenge as compared to other text analysis *** are several approaches to identify users’emotions fromthe conversational text for the English language,however regional or low resource languages have been *** Urdu language is one of them and despite being used by millions of users across the globe,with the best of our knowledge there exists no work on dialogue analysis in the Urdu ***,in this paper,we have proposed a model which utilizes deep learning and machine learning approaches for the classification of users’emotions from the *** accomplish this task,we have first created a dataset for the Urdu language with the help of existing English language datasets for dialogue *** that,we have preprocessed the data and selected dialogues with common *** the dataset is prepared,we have used different deep learning and machine learning techniques for the classification of *** have tuned the algorithms according to the Urdu language *** experimental evaluation has shown encouraging results with 67%accuracy for the Urdu dialogue datasets,more than 10,000 dialogues are classified into five emotions i.e.,joy,fear,anger,sadness,and *** believe that this is the first effort for emotion detection from the conversational text in the Urdu language domain.

关键词： Dialogue analysis conversational opinion mining sentiment analysis sentiment analysis in Urdu language deep learning machine learning

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Development of mutants with varying flowering times by targeted editing of multiple SVP gene copies in Brassica napus L.

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The Crop Journal 2022年第1期10卷 67-74页

作者： Sunny Ahmar Yungu Zhai Huibin Huang Kaidi Yu muhammad Hafeez ullah Khan muhammad Shahid rana Abdul Samad Shahid ullah Khan Olalekan Amoo Chuchuan Fan Yongming Zhou National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina

Manipulation of flowering time to develop cultivars with desired maturity dates is fundamental in plant *** is desirable to generate polyploid rapeseed(Brassica napus L.)germplasm with varying flowering time controlled by a few *** the present study,Bna SVP,a rapeseed homolog of the Arabidopsis SVP(Short Vegetative Phase)gene,was characterized and a set of mutants was developed using a CRISPR/Cas9-based gene-editing tool.A single construct targeting multiple sites was successfully applied to precisely mutate four copies of Bna *** induced mutations in these copies were stably transmitted to subsequent *** mutants with loss-of-function alleles and free transgenic elements were generated across the four Bna SVP *** mutant T_(1)lines tested in two environments(summer and winter growing seasons)showed early-flowering *** decrease in flowering time was correlated with the number of mutated Bna SVP *** quadruple mutants showed the shortest flowering time,with a mean decrease of 40.6%–50.7%in length relative to the wild type under the two growth *** study demonstrates the quantitative involvement of Bna SVP copies in the regulation of flowering time and provides valuable resources for rapeseed breeding.

关键词： Brassica napus Flowering time BnaSVP Gene editing

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Disintegration of uncertainties associated with real-time multi-satellite precipitation products in diverse topographic and climatic area in Pakistan

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Journal of Mountain Science 2021年第3期18卷 716-734页

作者： muhammad MASOOD Ghulam NABI muhammad BABUR Aftab Hussain AZHAR muhammad kaleem ullah Centre of Excellence in Water Resources Engineering University of Engineering&Technology GT-RoadLahore 54890Pakistan Civil Engineering Department University of Central PunjabLahore 54782Pakistan Directorate of Hydrology Pakistan Water&Power Development Authority(WAPDA)MDO-WAPDAMangla Dam10200Pakistan Civil Engineering Department University of LahoreLahore 54000Pakistan

Satellite-based Precipitation Estimates(SPEs)have gained importance due to enhanced spatial and temporal resolution,particularly in Indus basin,where raingauge network has fewer observation stations and drainage area is laying in many *** of SPEs is based on indirect mechanism,therefore,assessment and correction of associated uncertainties is *** the present study,disintegration of uncertainties associated with four prominent real time SPEs,IMERG,TMPA,CMORPH and PERSIANN has been conducted at grid level,regional scale,and summarized in terms of regions as well as whole study area *** bias has been disintegrated into hit,missed,false biases,and Root Mean Square Error(RMSE)into systematic and random errors.A comparison among gauge-and satellite-based precipitation estimates at annual scale,showed promising result,encouraging use of real time SPEs in the study *** grid basis,at daily scale,from box plots,the median values of total bias(-0.5 to 0.5 mm)of the used SPEs were also encouraging although some under/over estimations were noted in terms of hit bias(-0.15 to 0.05 mm/day).Relatively higher values of missed(0.3 to 0.5 mm/day)and false(0.5 to 0.7 mm/day)biases were *** detected average daily RMSE,systematic errors,and random errors were also comparatively ***-scale spatial distribution of uncertainties revealed lower values of uncertainties in plain areas,depicting the better performance of satellite-based products in these ***,in areas of high altitude(>4000 m),due to complex topography and climatic conditions(orographic precipitation and glaciated peaks)higher values of biases and errors were *** barriers and point scale gauge data could also be a cause of poor performance of SPEs in these areas,where precipitation is more on ridges and less in valleys where gauge stations are usually *** system’s size and intensity can also be a reason of higher biases,because M

关键词： Satellite precipitation Real-time Error characterization IMERG Indus Basin

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Study on the synthesis of spin labeled poly(styrene-co-maleic acid)s and their segmental motion

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Magnetic Resonance Letters 2022年第2期2卷 80-90,I0002页

作者： kaleem-Ur-Rahman Naveed Li Wang Haojie Yu Qian Zhang Wei Xiong Raja Summe ullah Ahsan Nazir muhammad Usman Shah Fahad Amin Khan Md Alim Uddin Di Shen State Key Laboratory of Chemical Engineering College of Chemical&Biological EngineeringZhejiang UniversityHangzhou310027China The First Affiliated Hospital College of MedicineZhejiang UniversityHangzhou310003China

Study of the segmental mobility of polymer chains is important when the polymer is used as drag reduction agents of crude *** spin resonance(ESR)spectroscopy can provide important information about segmental dynamics of polymer chains,which is related to their *** this article,we employed an amphiphilic polymer to study the effect of hydrophilic/hydrophobic balance of the polymers on the segmental motion of polymer ***(styrene-co-maleic acid)(PSMA)was spin labeled with 4-amino TEMPO radicals by increasing the concentration of radical moiety on the polymer *** PSMA and spin labeled-PSMAs(SL-PSMAs)were characterized by nuclear magnetic resonance(NMR),Fourier transform infrared(FT-IR)spectroscopy,Cyclic voltammetry(CV)and ESR ***-polymer complexes(IPCs)of SL-PSMA-2 were prepared by employing polyethylene glycols(PEGs)of varying molecular *** results showed that the increased hydrophobic interactions of nitroxide radicals on the SLPSMAs’chains reduced the rotational mobility of spin labels and the random coil-toglobular transition of polymer chains occurred at higher pH value for SL-PSMAs,which showed a slow motion component in the ESR spectra of ***,by increasing the molecular weight of PEGs in IPCs the complexation was increased,which also reduced the rotational motion of spin labels due to interpolymer hydrogen bonding causing a slow motion component in the ESR spectra.©2021 The Authors.

关键词： Electron spin resonance(ESR) Spin labeling Nitroxide radicals poly(styrene-co-maleic acid)s Segmental motion

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First COVID-19 related death in Pakistan in a patient with a travel history in Saudi Arabia

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Asian Pacific Journal of Tropical Medicine 2020年第8期13卷 375-377页

作者： Rooh ullah muhammad Suleman rana Mehmood Qadir muhammad Usman Department of Microbiology Quaid-i-Azam UniversityIslamabadPakistan Department of Virology National Institute of HealthIslamabadPakistan Atta ur Rahman School of Applied Biosciences National University of Science and TechnologyIslamabadPakistan

Rationale: Severe acute respiratory syndrome coronavirus-2(SARS-CoV-2) has been recognized as highly pathogenic. The current pandemic of SARS-CoV-2 has been spread globally and infected more than 200 countries. Patient concerns: We report the first confirmed fatal case of COVID-19 in Pakistan. A 50-year-old man returned from Saudi Arabia on March 09, 2020 and presented with cough, fever, malaise, poor appetite and difficulty in breathing to the Pulmonologist at District Headquarter Hospital Mardan. Diagnosis: The patient was initially diagnosed as COVID-19 suspected case. A oropharyngeal swab sample was positive by realtime RT-PCR tests. Lessons: This report highlights the importance of close coordination between clinicians and public health authorities as well as the importance of early laboratory-based confirmation of COVID-19 cases.

关键词： COVID-19 SARS-CoV-2 Pakistan

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Influence of rainfall infiltration on the stability of unsaturated coal gangue accumulated slope

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Journal of Mountain Science 2021年第6期18卷 1696-1709页

作者： kaleem ullah Jan KHAN WANG Chang-ming muhammad Wasim Jan KHAN LIANG Zhu LI Shuo LI Bai-long College of Construction Engineering Jilin UniversityChangchun 130021China Department of Management Sciences University College of Zhob(Sub-Campus BUITEMS)Balochistan 85200Pakistan

The slope instability is associated with increasing rate of rainfall infiltration which cause shear strength reduction and suction loss and the slope tend to failure. The influences of rainfall infiltration on the stability of clayey and sandy slopes have been analyzed but the effect of rainfall infiltration on the stability of unsaturated coal gangue accumulated slope was needed to study. Therefore, a coal gangue accumulated slope prone to failure in Fuxin area of Northeast China was considered to evaluate its failure mechanism under different rainfall events. The effects after five different rainfall events on slope stability were physically analyzed, numerically investigated and the results from both uncoupled(hydraulic) and coupled(hydromechanical) responses were compared using finite element analysis. It was observed that the decisive soaking and leaching under different rainfall conditions caused maximum deformation at the crest of slope due to maximum value of permeability coefficient of coal gangue. The critical duration of moderate intensity(147 mm/day) of rainfall for the instability of coal gangue accumulated slope is declared as five days. The results from finite element analysis in this paper further clarifies that increase in duration of rainfall infiltration process cause hysteretic change in positive pore-water pressure causing decrease in factor of safety and increase in deformation. It is concluded that the stability of unsaturated coal gangue accumulated slope is greatly influence by the coupled effect of stress and porewater pressure in comparison of uncoupled(hydraulic) analysis as the obtained factor of safety values after five days of rainfall infiltration were 0.9 and 1.1 respectively.

关键词： Hydro mechanical coupling Factor of safety Ground water table Rainfall infiltration Matric suction Coal gangue

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Identification of 1q21.1 microduplication in a family:A case report

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World Journal of Clinical Cases 2023年第4期11卷 874-882页

作者： Ting-Ting Huang Hai-Feng Xu Shang-Yu Wang Wen-Xin Lin Yie-Hen Tung kaleem ullah Khan Hui-HuiZhang Hu Guo Guo Zheng Gang Zhang Department of Neurology Children’s Hospital of Nanjing Medical UniversityNanjing 210000Jiangsu ProvinceChina Nanjing Medical University Nanjing 210000Jiangsu ProvinceChina Nanjing Xiaozhuang University Experimental Primary School Nanjing 210000Jiangsu ProvinceChina

BACKGROUND Copy number variation(CNV)has become widely recognized in recent years due to the extensive use of gene screening in developmental disorders and epilepsy research.1q21.1 microduplication syndrome is a rare CNV disease that can manifest as multiple congenital developmental disorders,autism spectrum disorders,congenital malformations,and congenital heart defects with genetic *** SUMMARY We reported a pediatric patient with 1q21.1 microduplication syndrome,and carried out a literature review to determine the correlation between 1q21.1microduplication and its *** summarized the patient’s medical history and clinical symptoms,and extracted genomic DNA from the patient,her parents,elder brother,and *** patient was an 8-mo-old girl who was hospitalized for recurrent convulsions over a 2-mo *** exon sequencing and whole genome low-depth sequencing(CNV-seq)were then *** exon sequencing detected a 1.58-Mb duplication in the CHR1:145883867-147465312 region,which was located in the 1q21.1 *** analysis showed that the pathogenetic duplication fragment,which was also detected in her elder brother’s DNA originated from the *** Whole exon sequencing combined with quantitative polymerase chain reaction can provide an accurate molecular diagnosis in children with 1q21.1 microduplication syndrome,which is of great significance for genetic counseling and early intervention.

关键词： 1q21.1 microduplication syndrome Epilepsy Copy number variation Familial Whole exon sequencing Congenital developmental disorders Case report

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