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Pancreatic stellate cell: Pandora's box for pancreatic disease biology

World Journal of Gastroenterology 2017年第3期23卷 382-405页

作者： Ratnakar R Bynigeri Aparna Jakkampudi ramaiah jangala Chivukula Subramanyam Mitnala Sasikala G Venkat Rao D Nageshwar Reddy Rupjyoti Talukdar Institute of Basic Sciences Asian Healthcare FoundationHyderabad 500082India Department of Medical Gastroenterology Asian Institute of GastroenterologyHyderabad 500082India Department of Surgical Gastroenterology Asian Institute of GastroenterologyHyderabad 500082India

Pancreatic stellate cells(PSCs) were identified in the early 1980 s, but received much attention after 1998 when the methods to isolate and culture them from murine and human sources were developed. PSCs contribute to a small proportion of all pancreatic cells under physiological condition, but are essential for maintaining the normal pancreatic architecture. Quiescent PSCs are characterized by the presence of vitamin A laden lipid droplets. Upon PSC activation, these perinuclear lipid droplets disappear from the cytosol, attain a myofibroblast like phenotype and expresses the activation marker, alpha smooth muscle actin. PSCs maintain their activated phenotype via an autocrine loop involving different cytokines and contribute to progressive fibrosis in chronic pancreatitis(CP) and pancreatic ductal adenocarcinoma(PDAC). Several pathways(e.g., JAK-STAT, Smad, Wnt signaling, Hedgehog etc.), transcription factors and mi RNAs have been implicated in the inflammatory and profibrogenic function of PSCs. The role of PSCs goes much beyond fibrosis/desmoplasia in PDAC. It is now shown that PSCs are involved in significant crosstalk between the pancreatic cancer cells and the cancer stroma. These interactions result in tumour progression, metastasis, tumour hypoxia, immune evasion and drug resistance. This is the rationale for therapeutic preclinical and clinical trials that have targeted PSCs and the cancer stroma.

关键词： Pancreatic stellate cells Pancreatic fibrosis Pancreatic cancer stroma Physiological functions Pancreatic stellate cells-cancer-stromal interactions Therapeutic targets

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New chiral reverse phase HPLC method for enantioselective analysis of ketorolac using chiral AGP column

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Journal of Pharmaceutical Analysis 2012年第6期2卷 462-465页

作者： Sunil K. Dubey jangala Hemanth Chiranjeevi Venkatesh K. R.N. Saha S. Pasha Department of Pharmacy Birla Institute of Technology & Science Pilani India Institute of Genomics and Integrative Biology Mall Road Delhi India

A simple, specific, precise, sensitive and rapid reverse phase-HPLC method was developed for determination of ketorolac enantiomers, a potent nonnarcotic analgesic in pharmaceutical formulations. The method was developed on a chiral AGP column. Mobile phase was 0.1 M sodium phosphate buffer （pH 4.5）： lsopropanol （98：2, v/v）, at a flow rate of 1 mL/min with run time of 15 min. Ultraviolet detection was made at 322 nm. The linearity range was 0.02 10 μg/mL for each of the enantiomers. The mobile phase composition was systematically studied to find the optimum chromatographic conditions. Validation of the method under the conditions selected showed that it was selective and precise and that the detector response was linear function of ketorolac.

关键词： R-KetorolacS-Ketorolac ChiralAGP RP-HPLC

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Method Development and Validation for Simultaneous Estimation of Montelukast Sodium and Desloratadine by RP-HPLC

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American Journal of Analytical Chemistry 2015年第8期6卷 651-658页

作者： Bonthu Mohan Gandhi Atmakuri Lakshmana Rao jangala Venkateswara Rao Sri Vasavi Institute of Pharmaceutical Sciences Tadepalligudem India V. V. Institute of Pharmaceutical Sciences Gudlavalleru India Bharat Institute of Pharmacy Mangalpally India

A novel, precise, accurate, rapid and cost effective isocratic reverse-phase high performance liquid chromatographic (RP-HPLC) method was developed, optimized and validated for the simultaneous estimation of Montelukast Sodium (MON) and Desloratadine (DES) in pharmaceutical dosage forms. The drugs were estimated using Hypersil BDS C18 (250 mm × 4.6 mm I.D., 5 μ particle size) column. The mobile phase composed of orthophosphoric acid and water in the ratio of 20:80 v/v, at a flow rate of 1.0 ml/min was used for the separation. Detection was carried out at 280 nm. The linearity range obtained was 10 - 30 μg/ml for MON and 5 - 15 μg/ml for DES with retention times of 2.929 min and 4.439 min for MON and DES respectively. The correlation coefficient values were found to be 0.999. Precision studies showed % RSD values less than 2% for both the drugs in all the selected concentrations. The percentage recoveries of MON and DES were in the range of 99.59% - 99.82% and 99.60% - 99.80% respectively. The limit of detection (LOD) and limit of quantification (LOQ) were 0.176 μg/ml, 0.587 μg/ml for MON and 0.087 μg/ml, 0.292 μg/ml for DES respectively. The method was validated as per the International Conference on Harmonization (ICH) guidelines. The proposed validated method was successfully used for the quantitative analysis of commercially available tablet dosage forms.

关键词： Montelukast Sodium Desloratadine HPLC Validation

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