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生物质资源与工程(英文) 2023年第2期8卷 108-124页

作者： Akansha Shrivastava Mamta Pal Rakesh kumar SHARMA Department of Biosciences Manipal University JaipurJaipur 303007India Sardar Swaran Singh National Institute of Bio-Energy Jalandhar 144601India

Yeast has been used as a cell factory for thousands of years to produce a wide variety of com-plex biofuels,bioproducts,biochemicals,food ingredients,and pharmaceuticals.For a variety of biotechnological production hosts,a few specific genera of yeast have proven themselves.Rapid developments in metabolic engineering and synthetic biology provide a workable long-term supply solution for these substances.In this review,we have covered recent advances in the design of yeast cell factories for the synthesis of terpenoids,alkaloids,phenylpropanoids,and other natural chemicals,primarily focusing on Pichia species.Cutting-edge solutions involving genetic and process engineering have also been discussed.Overall,the review summarized recent advancements and challenges in synthetic and systems biology,as well as initiatives in metabolic engineering aimed at commercializing non-conventional yeasts like Pichia.The processes used in non-traditional yeasts to produce enzymes,therapeutic proteins,lipids,and metabolic products for industrial applications were thoroughly elaborated.

关键词： Natural product Pichia Non-conventional yeast Biosynthesis System biology Synthetic biology

Vigna radiata var. GM4 Plant Growth Enhancement and Root Colonization by a Multi-Metal-Resistant Plant Growth-Promoting Bacterium Enterobacter sp. C1D in Cr(VI)-Amended Soils

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Pedosphere 2018年第1期28卷 144-156页

作者： Gangavarapu SUBRAHMANYAM Rakesh kumar SHARMA Gattupalli Naresh kumar Gattupalli ARCHANA Department of Microbiology and Biotechnology Centre The Maharaja Sayajirao University of Baroda Department of Biomedical Science Nitte University Center for Science Education and Research (NUCSER) Nitte University Central Muga Eri Research and Training Institute Central Silk Board Department of Biochemistry Faculty of Science The Maharaja Sayajirao University of Baroda

Contamination of agricultural soils by heavy metals has become a major concern due to their toxic effects on plant growth,symbiosis and consequently the yields of crops. In the present study, to enhance plant growth in Cr(VI)-amended soils, novel metalresistant plant growth-promoting bacteria(PGPB) were isolated from a soil contaminated with industrial waste effluent. One of the bacterial isolates, identified as Enterobacter sp. C1 D by 16 S r RNA gene sequencing, was found to be multi-metal resistant in nature with excellent plant growth-promoting(PGP) traits. Mung bean(Vigna radiata var. GM4) inoculation with Enterobacter sp.C1 D significantly(P < 0.01) increased root and shoot length, shoot and root weight, and chlorophyll content in a range of Cr(VI)treatments. Plant tolerance towards Cr(VI) measured as effective concentration showed higher values with Enterobacter sp. C1 Dtreated plants compared to un-inoculated plants. Root colonization study was also carried out using green fluorescence protein-labeled Enterobacter sp. C1 D under a hydroponic system. Confocal laser scanning microscopy of the plant roots showed heavy bacterial loads on the surface of the plant root specifically at the root tip and the point of root hair/lateral root formation. The results of PGP traits showed that elevated indole acetic acid levels and 1-aminocyclopropane-1-carboxylate deaminase activity enabled Enterobacter sp. C1 D to enhance V. radiata growth in Cr(VI)-amended soils, whereby it significantly increased plant tolerance towards elevated Cr(VI) concentrations.

关键词：植物生长农业土壤 var Cr sp 细菌修改激光扫描显微镜

Strategies to mitigate the adverse effect of drought stress on crop plants-influences of soil bacteria:A review

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Pedosphere 2021年第3期31卷 496-509页

作者： Priyanka CHANDRA Amoolya WUNNAVA Pooja VERMA Amaresh CHANDRA Rakesh kumar SHARMA Indian Council of Agricultural Research(ICAR) Central Soil Salinity Research InstituteKarnalHaryana 132001India Department of Biosciences Manipal University JaipurRajasthanJaipur 303007India Indian Council of Agricultural Research(ICAR) Indian Institute of Sugarcane ResearchUttar PradeshLucknow 226002India

Drought stress affects plant growth and causes significant issues in meeting global demand for food crops and fodder.Drought can cause physiological,physicochemical,and morphological changes in plants,which negatively affects plant growth and productivity.To combat this under the increasing global threat of water shortage and rapid population expansion,it is crucial to develop strategies to meet global food demands.Plant growth-promoting rhizobacteria(PGPR)may provide a safe solution to enhancing crop yields through various mechanisms.These soil bacteria can provide drought tolerance to crop plants,allowing them to survive and thrive in water-scarce conditions.Productions of phytohormones,free radical-scavenging enzymes,and stress-combating enzymes that can increase tolerance to drought-induced stress are key features of plant-associated microbial communities.This review summarizes the beneficial properties of microbes that help plants tolerate water scarcity and highlights the bacterial mechanisms that enhance drought tolerance in plants.

关键词： abiotic stress agricultural yield drought tolerance food crops microbe PGPR rhizobactoria water deficit

STABILITY AND BIFURCATION ANALYSIS OF A DELAYED INNOVATION DIFFUSION MODEL

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Acta Mathematica Scientia 2018年第2期38卷 709-732页

作者： rakesh kumar Anuj kumar sharma Kulbhushan A GNIHOTRI Department of Applied Sciences S.B.S. State Technical Campus Ferozepur Punjab 152004 India Research Scholar with I.K.G. Punjab Technical University Kapurthala Punjab 144603 India Department of Mathematics L.R.D.A. V. College Jagraon Ludhiana Punjab 142026 India

In this article, a nonlinear mathematical model for innovation diffusion with stage structure which incorporates the evaluation stage （time delay） is proposed. The model is analyzed by considering the effects of external as well as internal influences and other demographic processes such as emigration, intrinsic growth rate, death rate, etc. The asymptotical stability of the various equilibria is investigated. By analyzing the exponential characteristic equation with delay-dependent coefficients obtained through the variational matrix, it is found that Hopf bifurcation occurs when the evaluation period （time delay, T） passes through a critical value. Applying the normal form theory and the center manifold argument, we de- rive the explicit formulas determining the properties of the bifurcating periodic solutions. To illustrate our theoretical analysis, some numerical simulations are also included.

关键词： Innovation diffusion model stability analysis Hopf-bifurcation normal form theory center manifold theorem

DYNAMICAL BEHAVIOR OF AN INNOVATION DIFFUSION MODEL WITH INTRA-SPECIFIC COMPETITION BETWEEN COMPETING ADOPTERS

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Acta Mathematica Scientia 2022年第1期42卷 364-386页

作者： rakesh kumar Anuj kumar sharma Govind Prasad SAHU Department of Applied Sciences Shaheed Bhagat Singh State UniversityFerozepurPunjab 152004India Department of Mathematics L.R.D.A.V.CollegeJagraonLudhianaPunjab 142026India Center for Basic Sciences Pt Ravishankar Shukla UniversityRaipur(Chhattisgarh).India

In this paper,we proposed an innovation diffusion model with three compartments to investigate the diffusion of an innovation(product)in a particular region.The model exhibits two equilibria,namely,the adopter-free and an interior equilibrium.The existence and local stability of the adopter-free and interior equilibria are explored in terms of the effective Basic Influence Number(BIN)R_(A).It is investigated that the adopter free steady-state is stable if R_(A)<1.By consideringτ(the adoption experience of the adopters)as the bifurcation parameter,we have been able to obtain the critical value ofτresponsible for the periodic solutions due to Hopf bifurcation.The direction and stability analysis of bifurcating periodic solutions has been performed by using the arguments of normal form theory and the center manifold theorem.Exhaustive numerical simulations in the support of analytical results have been presented.

关键词： intra-specific competition basic influence number local stability Hopf-bifurcation normal form theory center manifold theorem

MOLECULAR DYNAMICS SIMULATION OF POLYMER/CARBON NANOTUBE COMPOSITES

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Acta Mechanica Solida Sinica 2015年第4期28卷 409-419页

作者： Sumit sharma rakesh Chandra Pramod kumar Navin kumar Department of Mechanical Engineering Dr.B.R.Ambedkar National Institute of Technology School of Mechanical Materials & Energy Engineering (SMMEE) Indian Institute of Technology

In this paper, we present classical molecular dynamics （MD） simulations of model polymer/CNT composites constructed by embedding a single wall （10,10） CNT into two different amorphous polymer matrices： poly（methyl methacrylate） and poly{（m-phenylene-vinylene）-co- [（2,5-dioctoxy-p-phenylene） vinylene]}, respectively, with different volume fractions. The simulation results support the idea that it is possible to use CNTs to mechanically reinforce an appropri- ate polymer matrix, especially in the longitudinal direction of the nanotube. The comparison of the simulation results with the macroscopic rule-of-mixtures for composite systems showed that for strong interfacial interactions, there can be large deviations of the results from the rule-of- mixtures. In order to verify this study, results obtained have been compared with those given by Elliott and Han （2007）.

关键词： nano-structures elasticity mechanical properties computational modeling

Establishment of magneto-dielectric effect and magneto-resistance in composite of PLT and Ba-based U-type hexaferrite

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Journal of Advanced Dielectrics 2023年第5期13卷 42-47页

作者： Kanika Aggarwal Madan sharma Shubhpreet Kaur Vishal Arora Indu sharma rakesh kumar Anupinder Singh Department of Physics Sant Longowal Institute of Engineering and Technology Longowal 148106PunjabIndia Multifunctional Materials Laboratory Department of PhysicsGuru Nanak Dev University Amritsar 143005PunjabIndia Department of Physics and Electronics Hindu College Amritsar 143001PunjabIndia Department of Physics Career Point UniversityHamirpur 176041 Himachal PradeshIndia Ludhiana Group of Colleges Affiliated by IKGPTU Kapurthala 140407 PunjabIndia

We are examining the behavior of a composite made of ferroelectrics with strong dielectric properties and ferrites with magnetic properties due to the rising need for materials for multipurpose devices.Many application avenues will be favored by the pairing of these materials’individual qualities with one another.Perovskite and U-type hexaferrite composite materials have been chosen for this study based on their magnetic and dielectric properties.The composites,(1−x)PLT−x(Ba1−3xNd2x)4Co2Fe36O60 where x=0.52,0.54,0.56,0.58 are prepared by solid state reaction method and phase formation was examined.Consideration has been given to the magnetic,dielectric,and magneto-dielectric properties.This work has established the link between the electric and magnetic domains while applying electric and magnetic forces to the materials.The magneto-dielectric research revealed magneto-electric coupling in all of the samples.However,the sample shows the maximum coupling for x=0.54 with MDR%values of 93.17%,which might be because this sample appears to have a high magneto-resistance(161%).

关键词： Perovskite U-type hexaferrite magneto-dielectric effect magneto-resistance response.

Defect induced room-temperature ferromagnetism and enhanced photocatalytic activity in Ni-doped ZnO synthesized by electrodeposition

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Chinese Physics B 2020年第10期29卷 566-572页

作者： Deepika Raju kumar Ritesh kumar Kamdeo Prasad Yadav Pratyush Vaibhav Seema sharma rakesh kumar Singh Santosh kumar College o f Commerce Arts and SciencePatna.BiharIndia Jaypee University o f Engineering and Technology GunaMadhya PradeshIndia Anugrah Narayan College PatnaBiharIndia Aryabhatta Knowledge University PatnaBiharIndia

Zn0.90Ni0.10O nanoparticles have been synthesized by single-bath two-electrode electrodeposition at constant voltage. X-ray diffraction, UV vis and photoluminescence studies reveal that a single-phase polycrystalline hcp wurtzite crystal structure of ZnO is evolved. The material consists of a large number of defects such as oxygen vacancy (Ov) and zinc interstitial (Zi). The magnetization study reveals that the sample exhibits room-temperature global ferromagnetism and the ferromagnetic ordering seems to be defect induced via bound magnetic polaron mechanism, and double exchange is also expected to have played role. Interesting optoelectronic properties have been found in the synthesized sample and the material seems to be a potential candidate to be used as a UV sensor. Such a transition metal doped ZnO based dilute magnetic semiconducting system exhibiting room-temperature ferromagnetism is likely to be first of its kind in the sense that such materials have not yet been reported to be synthesized by the simple method of electrodeposition to the best of our knowledge on the basis of ample literature review.

关键词： dilute magnetic semiconductors(DMS) bound magnetic polaron photoluminescence ferromagnetism

Low-cost high entropy alloy(HEA)for high-efficiency oxygen evolution reaction(OER)

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Nano Research 2022年第6期15卷 4799-4806页

作者： Lalita sharma Nirmal kumar Katiyar Arko Parui rakesh Das Ritesh kumar Chandra Sekhar Tiwary Abhisek K.Singh Aditi Halder Krishanu Biswas School of Basic Sciences Advanced Materials Research Centre(AMRC)Indian Institute of Technology Mandi(H.P)KamandMandi 175005India Department of Materials Science and Engineering Indian Institute of Technology KanpurKanpur 208016India School of Engineering London South Bank UniversityLondonSE10 AAUK Materials Research Centre Indian Institute of ScienceBangalore 560012India Metallurgical and Materials Engineering Indian Institute of Technology KharagpurKharagpur 382355India

Oxygen evolution reaction(OER)is the key step involved both in water splitting devices and rechargeable metal-air batteries,and hence,there is an urgent need for a stable and low-cost material for efficient OER.In the present investigation,Co-Fe-Ga-NiZn(CFGNZ)high entropy alloy(HEA)has been utilized as a low-cost electrocatalyst for OER.Herein,after cyclic voltammetry activation,CFGNZ-nanoparticles(NPs)are covered with oxidized surface and form high entropy(oxy)hydroxides(HEOs),exhibiting a low overpotential of 370 mV to achieve a current density of 10 mA/cm^(2)with a small Tafel slope of 71 mV/dec.CFGNZ alloy has higher electrochemical stability in comparison to state-of-the art RuO2 electrocatalyst as no degradation has been observed up to 10 h of chronoamperometry.Transmission electron microscopy(TEM)studies after 10 h of long-term chronoamperometry test showed no change in the crystal structure,which confirmed the high stability of CFGNZ.The density functional theory(DFT)based calculations show that the closeness of d(p)-band centers to the Fermi level(EF)plays a major role in determining active sites.This work highlights the tremendous potential of CFGNZ HEA for OER,which is the primary reaction involved in water splitting.

关键词： high entropy alloy cyclic voltammetry activation oxygen evolution reaction electrocatalyst nanocrystalline catalyst

Targeting brain tumors with innovative nanocarriers:bridging the gap through the blood-brain barrier

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Oncology Research 2024年第5期32卷 877-897页

作者： KARAN WADHWA PAYAL CHAUHAN SHOBHIT kumar rakesh PAHWA RAVINDER VERMA RAJAT GOYAL GOVIND SINGH ARCHANA sharma NEHA RAO DEEPAK KAUSHIK Department of Pharmaceutical Sciences Maharshi Dayanand UniversityRohtak124001India Department of Pharmaceutical Technology Meerut Institute of Engineering and Technology(MIET)NH-58Delhi-Roorkee HighwayMeerut250005India Institute of Pharmaceutical Sciences Kurukshetra UniversityKurukshetra136119India Department of Pharmaceutical Sciences Chaudhary Bansi Lal UniversityBhiwani127021India MM College of Pharmacy Maharishi Markandeshwar(Deemed to be University)Mullana-AmbalaHaryana133207India Delhi Pharmaceutical Sciences and Research University(DIPSAR) Delhi Pharmaceutical Sciences and Research UniversityNew Delhi110017India

Background:Glioblastoma multiforme(GBM)is recognized as the most lethal and most highly invasive tumor.The high likelihood of treatment failure arises fromthe presence of the blood-brain barrier(BBB)and stemcells around GBM,which avert the entry of chemotherapeutic drugs into the tumormass.Objective:Recently,several researchers have designed novel nanocarrier systems like liposomes,dendrimers,metallic nanoparticles,nanodiamonds,and nanorobot approaches,allowing drugs to infiltrate the BBB more efficiently,opening up innovative avenues to prevail over therapy problems and radiation therapy.Methods:Relevant literature for this manuscript has been collected from a comprehensive and systematic search of databases,for example,PubMed,Science Direct,Google Scholar,and others,using specific keyword combinations,including“glioblastoma,”“brain tumor,”“nanocarriers,”and several others.Conclusion:This review also provides deep insights into recent advancements in nanocarrier-based formulations and technologies for GBM management.Elucidation of various scientific advances in conjunction with encouraging findings concerning the future perspectives and challenges of nanocarriers for effective brain tumor management has also been discussed.

关键词： Glioblastoma Brain tumor Blood-brain barrier Liposomes Metallic nanoparticles Nanocarriers