Vigna radiata var. GM4 Plant Growth Enhancement and Root Colonization by a Multi-Metal-Resistant Plant Growth-Promoting Bacterium Enterobacter sp. C1D in Cr(VI)-Amended Soils

作     者：Gangavarapu SUBRAHMANYAM Rakesh Kumar SHARMA Gattupalli Naresh KUMAR Gattupalli ARCHANA 

作者机构：Department of Microbiology and Biotechnology Centre The Maharaja Sayajirao University of Baroda Department of Biomedical Science Nitte University Center for Science Education and Research (NUCSER) Nitte University Central Muga Eri Research and Training InstituteCentral Silk Board Department of Biochemistry Faculty of Science The Maharaja Sayajirao University of Baroda 

出 版 物：《Pedosphere》 (土壤圈（英文版）)

年 卷 期：2018年第28卷第1期

页      面：144-156页

核心收录：

学科分类：082803[工学-农业生物环境与能源工程] 07[理学] 08[工学] 0828[工学-农业工程] 0818[工学-地质资源与地质工程] 0903[农学-农业资源与环境] 0901[农学-作物学] 0713[理学-生态学] 

基　　金：supported by the Department of Science and Technology (DST)  Government of India (No. SR/S4/ES-21/Baroda Window/P3) 

主　　题：植物生长 农业土壤 var Cr sp 细菌 修改 激光扫描显微镜 

摘      要：Contamination of agricultural soils by heavy metals has become a major concern due to their toxic effects on plant growth,symbiosis and consequently the yields of crops. In the present study, to enhance plant growth in Cr(VI)-amended soils, novel metalresistant plant growth-promoting bacteria(PGPB) were isolated from a soil contaminated with industrial waste effluent. One of the bacterial isolates, identified as Enterobacter sp. C1 D by 16 S r RNA gene sequencing, was found to be multi-metal resistant in nature with excellent plant growth-promoting(PGP) traits. Mung bean(Vigna radiata var. GM4) inoculation with Enterobacter sp.C1 D significantly(P 0.01) increased root and shoot length, shoot and root weight, and chlorophyll content in a range of Cr(VI)treatments. Plant tolerance towards Cr(VI) measured as effective concentration showed higher values with Enterobacter sp. C1 Dtreated plants compared to un-inoculated plants. Root colonization study was also carried out using green fluorescence protein-labeled Enterobacter sp. C1 D under a hydroponic system. Confocal laser scanning microscopy of the plant roots showed heavy bacterial loads on the surface of the plant root specifically at the root tip and the point of root hair/lateral root formation. The results of PGP traits showed that elevated indole acetic acid levels and 1-aminocyclopropane-1-carboxylate deaminase activity enabled Enterobacter sp. C1 D to enhance V. radiata growth in Cr(VI)-amended soils, whereby it significantly increased plant tolerance towards elevated Cr(VI) concentrations.