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现代装饰 2023年第4期 54-59页

作者： rafael ponce ortiz Margarita Gorbea Angeles Cesar Avila OscarDiaz Gaspar Abigail Esparza Diego Buenode la Paz Valerio Lopez Acevedo Margarita Gorbea(摄影) Marcos Betanzos(摄影) 范嘉苑(编译) 不详

“环境文化中心”作为墨西哥城Bosquede Chapultepec第二部分的全新文化、景观和环境节点,是由Gabriel Orozco操刀的“查普特佩克自然与文化”总体规划的一部分。该项目方案在墨西哥城政府与联邦政府共同举办的公开竞赛中脱颖而出,得以... 详细信息

“环境文化中心”作为墨西哥城Bosquede Chapultepec第二部分的全新文化、景观和环境节点,是由Gabriel Orozco操刀的“查普特佩克自然与文化”总体规划的一部分。该项目方案在墨西哥城政府与联邦政府共同举办的公开竞赛中脱颖而出,得以落成。环境文化中心是一座被植物花园包围的展馆,展现出自然主义设计与民族植物学相结合的理念。设计参考了墨西哥盆地山谷的多样化生态系统和自然景观,包括温带森林、草原、湿地和植被等,并与查普特佩克现有的植被、地形和设施进行细致整合,由此最大化地增加环境文化中心作为城市森林的巨大潜力。

关键词：墨西哥城设计参考温带森林民族植物学自然景观生态系统自然与文化总体规划

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Arsenic and chromium resistance mechanisms in the Micrococcus luteus group

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Pedosphere 2023年第4期33卷 600-611页

作者： Ivan ARROYO-HERRERA Brenda ROMÁN-ponce rafael BUSTAMANTE-BRITO Joseph GUEVARA-LUNA Erika Yanet TAPIA-GARCÍA Violeta LARIOS-SERRATO Nannan ZHANG Paulina ESTRADA-DE LOS SANTOS En Tao WANG María Soledad VÁSQUEZ-MURRIETA Escuela Nacional de Ciencias Biológicas Instituto Politécnico NacionalCiudad de México 11340(Mexico) Universidad Politécnica del Estado de Morelos Jiutepec 62550(Mexico) Centro de Ciencias Genómicas Universidad Autónoma de MéxicoCuernavaca 62210(Mexico) CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilisation&Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province Chengdu Institute of BiologyChinese Academy of SciencesChengdu 610041(China)

High arsenic(As)and chromium(Cr)concentrations are currently receiving attention because of their negative effects on the environment and human health.Microorganisms inhabiting contaminated environments have developed resistance mechanisms against the toxicity of these pollutants.Indeed,members of the bacterial genus Micrococcus have been isolated from different toxic metal-contaminated environments;however,knowledge concerning its resistance mechanisms to As and Cr toxicity remains limited.Micrococcus luteus strains(An24,Mh,NE2TL6,and NE2TTS4)were isolated from the endosphere and soil of two heavy metal-contaminated sites in Mexico to identify differences in the resistance mechanisms by the M.luteus group.The strains were resistant to As(As^(3+)and As^(5+)),chromate,dichromate,cobalt,copper,nickel,and zinc.Genome analysis indicated that the heavy metal-resistant strains(An24,Mh,NE2TL6,and NE2TTS4)could be assigned to the M.luteus group and had more heavy metal-resistant genes(transporters,chaperones,and enzymes)compared to reference strains of the M.luteus group,M.luteus NCTC 2665^(T)and Micrococcus endophyticus JCM 16951^(T).The resistant bacteria were able to biotransform As^(3+)and As^(5+)through a carbon source-dependent mechanism.The biotransformation of As5+was potentially carried out in the cytoplasm through a thioredoxin-dependent pathway,which may be coupled with biosorption.A qualitative analysis of organic acids(OAs)identified a change in the OA profile of the metal-resistant strains that was As-or Cr-dependent.Our genomic and phenotypic findings suggest that the four M.luteus group strains evaluated in the current study have developed resistance mechanisms that may enable their survival in contaminated sites.

关键词： arsenophore biosorption genome analysis heavy metal-resistant strain immobilization organic acid pollutant

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Serum levels of chemokines in adolescents with major depression treated with fluoxetine

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World Journal of Psychiatry 2020年第8期10卷 175-186页

作者： Francisco rafael de la Peña Carlos Cruz-Fuentes Lino Palacios Manuel Iván Girón-Pérez Emilio Medina-Rivero Maria Dolores ponce-Regalado Samantha Alvarez-Herrera Gilberto Pérez-Sánchez Enrique Becerril-Villanueva JoséLuis Maldonado-García María C Jiménez-Martínez Lenin Pavón Adolescent Clinic Clinical ServicesNational Institute of Psychiatry“Ramón de la Fuente”Mexico City 14370Mexico Department of Psychiatric Genetics Clinical Research BranchNational Institute of Psychiatry“Ramón de la Fuente”Mexico City 14370Mexico Universidad Autónoma de Nayarit Laboratorio de InmunotoxicologíaBoulevard Tepic-Xalisco s/nCd de la Cultura Amado NervoTepic 63000Mexico Centro Nayarita de Innovación y Transferencia de Tecnología A.C.Laboratorio Nacional para la Investigación en Inocuidad Alimentaria-Unidad Nayarit Calle Tres s/n.Cd IndustrialTepic 63000NayaritMexico Unidad de Desarrollo e Investigación en Bioprocesos Escuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalUnidad Profesional Lázaro CárdenasMexico City 11340Mexico Departamento de Ciencias de Salud Centro Universitario de los AltosUniversidad de Guadalajara Av Rafael Casillas Aceves No.1200Tepatitlán de MorelosJalisco47610Mexico Laboratory of Psychoimmunology National Institute of Psychiatry“Ramón de la Fuente”Mexico City 14370Mexico Department of Immunology and Research Unit Institute of Ophthalmology“Conde de Valenciana Foundation”Mexico City 06800Mexico Department of Biochemistry Faculty of MedicineNational Autonomous University of MexicoMexico City 04510Mexico

BACKGROUND Major depressive disorder(MDD)is a global health issue that affects 350 million people of all ages.Although between 2%and 5.6%of affected individuals are adolescents,research on young patients is limited.The inflammatory response contributes to the onset of depression,and in adult MDD patients,symptom severity has been linked to chemokine levels.AIM To determine the differences in circulatory levels of chemokines in healthy volunteers(HVs)and adolescents with MDD,and assess the changes induced by fluoxetine consume.METHODS The 22 adolescents with MDD were monitored during the first 8 wk of clinical follow-up and clinical psychiatric evaluation was done using the Hamilton depresión rating scale(HDRS).The serum levels of monocyte chemoattractant protein-1(MCP-1),macrophage inflammatory protein(MIP)-1α,MIP-1β,interleukin(IL)-8,interferon gamma-induced protein(IP)-10,and eotaxin were measured in patients and HVs.RESULTS In all cases,significant differences were detected in circulating chemokine levels between patients before treatment and HVs(P<0.0001).All chemokines decreased at 4 wk,but only MCP-1 and IL-8 significantly differed(P<0.05)between 0 wk and 4 wk.In the patients,all chemokines rose to their initial concentrations by 8 wk vs 0 wk,but only IP-10 did so significantly(P<0.05).All patients experienced a significant decrease in HDRS scores at 4 wk(P<0.0001)and 8 wk(P<0.0001)compared with 0 wk.CONCLUSION Despite the consumption of fluoxetine,patients had significantly higher chemokine levels,even after considering the improvement in HDRS score.The high levels of eotaxin,IP-10,and IL-8 partially explain certain aspects that are affected in MDD such as cognition,memory,and learning.

关键词： Adolescents Major depression Chemokine Fluoxetine Interferon gammainduced protein-10

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