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Virulence Factors and Biofilm Formation in Vancomycin Resistant Enterococcus faecalis and Enterococcus faecium Isolates in Brazil

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Advances in Microbiology 2023年第6期13卷 299-314页

作者： Bárbara de Azevedo Ramos Marília Manta Manta sivoneide Maria da silva rafael artur queiroz cavalcanti de sá Natália Lira de souza Henrique Douglas Melo Coutinho Márcia Vanusa da silva Túlio Diego da silva Maria Tereza dos santos Correia Maria Betânia Melo de Oliveira Universidade Federal de Pernambuco Pernambuco Brazil Universidade Regional do Cariri Ceará Brazil Instituto de Tecnologia de Pernambuco Pernambuco Brazil

In this work, we evaluated biofilm formation of Vancomycin Resistant of E. faecalis and E. faecium (VRE) in different culture media and adhesion substrate, as well as cellular hydrophobicity and presence of virulence genes. For this, 35 isolates were collected from a public hospital in Recife, Pernambuco, Brazil and identified by the Matrix-Assisted Laser desorption Ionization - Time-of-flight - Mass spectrometry (MALDI-TOF-Ms) technique. Biofilm formation was analyzed by the Crystal Violet (CV) method and fluorescence microscopy, cellular hydrophobicity by hydrocarbon interaction and the presence of gelE, esp and asa1 genes by Polymerase Chain Reaction (PCR). 12 isolates were identified as E. faecalis and 23 as E. faecium. Most were obtained in Coronary Units (40.0%) and Intensive Care Unit (31.4%). E. faecium isolates were more resistant to the antibiotics tested than E. faecalis;however, E. faecalis stood out as a biofilm producer. Regarding the presence and gene frequency, it was observed that gelE (54.3%) and esp (54.3%) were the most prevalent, followed by asa1 (22.9%). When comparing the gene frequency, it was observed that gelE and esp were predominant (48.6% for both species), while asa1 was more frequent in E. faecalis (20.0%). The data presented here are worrying, because they reveal the virulence potential of isolates VRE, which contributes to the dissemination and persistence of these pathogens in the hospital environment.

关键词： Biofilm Cellular Hydrophobicity Gram-Positive Hospital Environment Virulence Genes

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Aquatic Microbiota: A systematic Review

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Advances in Microbiology 2023年第6期13卷 284-298页

作者： Karolayne silva souza Milena Roberta Freire da silva Ilton Palmeira da silva Lívia Caroline Alexandre de Araújo rafael artur de queiroz cavalcanti de sá Fabricio Motteran Bruno Oliveira de Veras Maria Betânia Melo de Oliveira departamento de Bioquímica Universidade Federal de Pernambuco—UFPE Recife Brazil Centro Universitário do River são Francisco—UniRios Paulo Afonso Brazil

Freshwater environments are the main ecosystems subjected to various anthropogenic impacts, which have caused the waterborne transmission of diseases caused mainly by bacteria. The main objective of this work was to carry out a systematic analysis through the literature on the main microorganisms found in impacted aquatic environments and to relate the data with the risks to public health. This review was planned, conducted and reported following the PRIsMA protocol, which was used the PECO strategy. There performed searches for studies using the electronic databases PubMed, Medline and LILACs in september 2021, using the controlled search strategy: “Bacteria” and “Ecosystem” and “Environment” and “Aquatic” and “Impacted. The studies that met the eligibility criteria were considered eligible, with the participation of two independent reviewers. There found 1058 studies, of which were chosen only 31 to compose the results of this systematic review. Because of the results, there observed that the predominant bacterial genera in freshwater are: Pseudomonas, Aeromonas, and Escherichia, with the Asian continent presenting more varieties of bacterial species detected. These microorganisms are responsible for causing diseases that can lead to death, which shows the importance of constantly monitoring these ecosystems.

关键词： Water Microorganisms Pathogenicity Monitoring

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Biotechnological Potential of Carotenoids Produced by Extremophilic Microorganisms and Application Prospects for the Cosmetics Industry

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Advances in Microbiology 2020年第8期10卷 397-410页

作者： Tayane de Cássia Dias Mendes-silva Rosileide Fontenele da silva Andrade Marcio Akio Ootani Paulo Vitor Dias Mendes rafael artur de queiroz cavalcanti de sá Milena Roberta Freire da silva Karolayne silva souza Maria Tereza dos santos Correia Márcia Vanusa da silva Maria Betânia Melo de Oliveira department of Biochemistry Federal University of Pernambuco Recife Brazil National Program Postdoctoral CAPEs (PNPD) Catholic University of Pernambuco Recife Brazil Bioprocess Laboratory Center of Strategic Technologies Northeast Recife Brazil design department Federal University of Pernambuco Recife Brazil Nucleus of Bioprospecting and Conservation of the Caatinga Semiarid National Institute/Ministry of Science Technology Innovations and Communications Campina Grande Brazil

Carotenoids are isoprenoid pigments used in food, chemical, textile, pharmaceutical, and cosmetic industries. They act not only as dyes and provitamins A but also have antioxidants, photoprotective, antimicrobial pr... 详细信息

style="text-align:justify;"> Carotenoids are isoprenoid pigments used in food, chemical, textile, pharmaceutical, and cosmetic industries. They act not only as dyes and provitamins A but also have antioxidants, photoprotective, antimicrobial properties, among others. This class of pigment can be obtained traditionally by plants or chemical synthesis, but they have some disadvantages. In recent years, search for alternative sources has been an important strategy for the carotenoid industries. Microbial synthesis is an alternative that has shown good yields, speed, and reduced production costs. Hostile environments, such as the Caatinga domain, represent an interesting source of microorganisms that produce biomolecules, especially carotenoids, because of oxidative stress caused by sunlight. Thus, this region has been attracting the attention of the scientific community and industry for the use of these organisms in the production of carotenoids and applications in cosmetic products;since these compounds have interesting antioxidant and photoprotective properties. In this review, general characteristics of carotenoids, sources of production, industrial applicability, and commercialization will be discussed, as well as perspectives on the production of carotenoids from microorganisms isolated from the Caatinga and their application in anti-UV products.span> span>

关键词： Natural Pigment Microbial Production Hostile Environments Antioxidant Photoprotection

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Performance of a serological IgM and IgG qualitative test for COVID-19 diagnosis:An experimental study in Brazil

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World Journal of Experimental Medicine 2022年第5期12卷 100-103页

作者： Fabrício Freire de Melo Lilian Martins Oliveira Diniz JoséNélio Januário João Fernando Gonçalves Ferreira rafael santos Dantas Miranda Dórea Breno Bittencourt de Brito Hanna santos Marques Fabian Fellipe Bueno Lemos Marcel silva Luz samuel Luca Rocha Pinheiro Dulciene Maria de Magalhães queiroz Instituto Multidisciplinar em saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil departamento de Pediatria Universidade Federal de Minas GeraisBelo Horizonte 31270-901Brazil Núcleo de Ações e Pesquisa em Apoio Diagnóstico Universidade Federal de Minas GeraisBelo Horizonte 31270-901Brazil Laboratory of Research in Bacteriology Universidade Federal de Minas GeraisBelo Horizonte 31270-901Brazil Laboratory of Bacteriology Faculdade de MedicinaUniversidade Federal de Minas GeraisBelo Horizonte 31270-901Brazil Campus Vitória da Conquista Universidade Estadual do Sudoeste da BahiaVitória da Conquista 45029-094Brazil

Qualitative antibody tests are an easy,point-of-care diagnostic method that is useful in diagnosing coronavirus disease 2019,especially in situations where reverse transcription-polymerase chain reaction is negative.However,some factors are able to affect its sensitivity and accuracy,which may contribute to these tests not being used as a first-line diagnostic tool.

关键词： serological test IgM IgG COVID-19 Diagnosis Antibody

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Epidemiological and clinical characteristics of COVID-19 in a Brazilian public hospital

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World Journal of Clinical Cases 2023年第8期11卷 1761-1770页

作者： Fillipe Dantas Pinheiro Luana Weber Lopes rafael santos Dantas Miranda Dórea Glauber Rocha Lima Araújo Filipe Antônio França da silva Breno Bittencourt de Brito Maria Luísa Cordeiro santos Geovani Moreno santos Júnior Maria Teresa Araújo de Lorenzo Barcia Renata de Amorim Marques AndréBezerra Botelho Anna Carolina saúde Dantas Davi Tanajura Costa Adriano Fernandes Teixeira Cláudio Lima souza Lucas Miranda Marques Guilherme Barretos Campos Márcio Vasconcelos Oliveira Dulciene Maria de Magalhães queiroz Fabrício Freire de Melo Instituto Multidisciplinar em saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil Hospital Geral de Vitória da Conquista Vitória da Conquista 45030-160BahiaBrazil departamento de Ciências da saúde Universidade Estadual do Sudoeste da Bahia(UESB)Vitória da Conquista 45083-900BahiaBrazil Faculdade de Medicina Universidade Federal de Minas GeraisBelo Horizonte 31270-901Minas GeraisBrazil

BACKGROUND The coronavirus disease 2019(COVID-19)pandemic has become a major health concern worldwide.In that context,the understanding of epidemiological and clinical features associated with the disease and its severity is crucial for the establishment of strategies aimed at disease control and remedy.AIM To describe epidemiological features,signs,symptoms,and laboratory findings among severely ill COVID-19 patients from an inten-sive care unit in northeastern Brazil as well as to evaluate predictor factors for disease outcomes.METHODs This is a prospective single-center study that evaluated 115 patients admitted to the intensive care unit in a northeastern Brazilian hospital.REsULTs The patients had a median age of 65.60±15.78 years.Dyspnea was the most frequent symptom,affecting 73.9%of the patients,followed by cough(54.7%).Fever was reported in approximately one-third of patients and myalgia in 20.8%of the patients.At least two comorbidities were found in 41.7%of the patients,and hypertension was the most prevalent(57.3%).In addition,having two or more comorbidities was a predictor of mortality,and lower platelet count was positively associated with death.Nausea and vomiting were two symptoms that were predictors of death,and the presence of a cough was a protective factor.CONCLUsION This is the first report of a negative correlation between cough and death in severely ill severe acute respiratory syndrome coronavirus 2-infected individuals.The associations between comorbidities,advanced age,and low platelet count and the outcomes of the infection were similar to the results of previous studies,highlighting the relevance of these features.

关键词： COVID-19 Epidemiology symptoms Comorbidities Laboratory parameters

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Lafoensia pacari alleviates intestinal damage by modulating cyclooxygenase-2:In silico and in vivo evaluation in a colitis model

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World Journal of Gastroenterology 2023年第17期29卷 2628-2641页

作者： Gabrielle Caroline Peiter Thayene Kamyli Moesch queiroz Edson Luiz Michalkiewicz Jr Raphael Henrique Chappuis Jennefer sousa Luz Luiz Henrique Casagrande Piovezani Cleison Ferreira silva Matheus Nozomi Tsutumi Augusto Fernandes Chaves rafael Messias Luiz Cinthia Façanha Wendel Ana Carla Zarpelon-schutz Kádima Nayara Teixeira Programa Multicêntrico de Pós-graduação em Biquímica e Biologia Molecular-setor Palotina Universidade Federal do ParanáPalotina 85.950-000ParanáBrazil Programa de Pós-graduação em Biotecnologia-setor Palotina Universidade Federal do ParanáPalotina 85.950-000ParanáBrazil Campus Toledo Universidade Federal do ParanáToledo 85.919-899ParanáBrazil

BACKGROUND Inflammatory bowel diseases(IBD)are a worldwide health problem and mainly affect young people,consequently affecting the workforce.Available treatments are often associated with side effects,and new therapeutic options are needed.For centuries,plants have represented important substrates in the field of drug development.Lafoensia pacari(L.pacari)is a plant whose pharmaceutical potential has been described,and may have biological activity relevant to the treatment of IBD symptoms.AIM To investigate the activity of keto-alcoholic extracts of L.pacari with respect to ameliorating the inflammatory and nociceptive symptoms of acute experimental colitis in mice.METHODs Keto-alcoholic extracts of L.pacari leaves and bark were administered to male andfemale swiss mice weighing 25 g to 30 g(n=8 male mice and n=8 female mice).The effect of these extracts was observed in an acetic acid-induced acute experimental model of colitis with regard to antinociception/analgesia and inflammatory tissue damage.Recorded macroscopic indices included the Wallace score and the colon weight obtained using a precision scale.Mechanical hyperalgesia was determined using an electronic analgesimeter.Behavior related to overt pain was determined by quantifying the number of writhing instances within 20 min of administration of acetic acid.Molecular docking was performed using human and murine cyclooxygenase-2(COX-2)with 3 flavonoids(ellagic acid,kaempferol,and quercetin)on the AutoDock Vina software.Analysis of variance followed by Tukey’s posttest was used with Psignificance.REsULTs In this murine model of colitis,administration of extracts from L.pacari ameliorated acetic acidinduced writhing and colitis-associated inflammatory pain.These improvements may be attributable to the reduction in edema,inflammation(e.g.,ulcers,hyperemia,and bowel wall damage),and the intensity of abdominal hyperalgesia.The keto-alcoholic extracts of L.pacari leaves and bark administered at a dose of either 100 mg/kg or 300 mg/kg significa

关键词： Antinociceptive activity Anti-inflammatory activity Cyclooxygenase-2 Flavonoids Inflammatory bowel disease Lafoensia pacari

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Correction to“Performance of a serological IgM and IgG qualitative test for COVID-19 diagnosis:An experimental study in Brazil”

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World Journal of Experimental Medicine 2023年第3期13卷 47-49页

作者： Fabrício Freire de Melo Lilian Martins Oliveira Diniz JoséNélio Januário João Fernando Gonçalves Ferreira rafael santos Dantas Miranda Dórea Breno Bittencourt de Brito Hanna santos Marques Fabian Fellipe Bueno Lemos Marcel silva Luz samuel Luca Rocha Pinheiro Gifone Aguiar Rocha Dulciene Maria de Magalhães queiroz Instituto Multidisciplinar em saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil departamento de Pediatria Universidade Federal de Minas GeraisBelo Horizonte 30130-100Minas GeraisBrazil departamento de Clínica Médica Universidade Federal de Minas GeraisBelo Horizonte 30130-100Minas GeraisBrazil Laboratory of Research in Bacteriology Universidade Federal de Minas GeraisBelo Horizonte 30130-100Minas GeraisBrazil Laboratory of Bacteriology Faculdade de MedicinaUniversidade Federal de Minas GeraisBelo Horizonte 30130-100Brazil Campus Vitória da Conquista Universidade Estadual do Sudoeste da BahiaVitória da Conquista 45029-094BahiaBrazil

Correction to“Freire de Melo F,Martins Oliveira Diniz L,Nélio Januário J,Fernando Gonçalves Ferreira J,Dórea RsDM,de Brito BB,Marques Hs,Lemos FFB,silva Luz M,Rocha Pinheiro sL,de Magalhães queiroz DM.Performance of a serological IgM and IgG qualitative test for COVID-19 diagnosis:An experimental study in Brazil.World J Exp Med 2022;12(5):100-103[PMID:36196438 DOI:10.5493/wjem.v12.i5.100]”.In this article,we identified an issue with the“Acknowledgments”section.Here,we then provide a recognition section for our supporting institutions.

关键词： Correction COVID-19 serological testing serologic tests Rapid diagnostic

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Effects of the Orientation of the Mountainside, Altitude and Varieties on the Quality of the Coffee Beverage from the “Matas de Minas” Region, Brazilian southeast

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American Journal of Plant sciences 2016年第8期7卷 1291-1303页

作者： Williams P. M. Ferreira Daniel M. queiroz samuel A. silvac rafael simões Tomaz Paulo C. Corrêa National Coffee Research Center Brazilian Corporation of Agricultural Research—EMBRAPA Vicosa Brazil departament of Agricultural Engineering Federal University of Vicosa—UFV Vicosa Brazil department of Rural Engineering Federal University of Espirito Santo—UFES Alegre Brazil department of Agronomy Engineering Faculdade de Ciencias Agrárias e Tecnológicas (FCAT) Sao Paulo State University—UNESP Dracena Brazil

Based upon qualitative parameters experiments, this study aims to investigate how the elements of the environment, where the coffee is produced, contribute to the final quality of the product. For the analyses, it was used approximately one kilogram of coffee cherry samples collected in 14 municipalities previously chosen on the East side of the Minas Gerais state, Brazil. The coffee cherry samples were collected and analyzed for each of the two varieties in four levels of altitude for each exposure side of the mountain in relation to the sun. The quality of the coffee was evaluated through the analysis of its physical characteristics and sensory analysis, popularly known as "Test of drink or Cupping" carried out by three tasters that belonging to the group of Q-Graders, according to the rules of national and international competitions of the Brazilian Association of special Coffees (BsCA). Were performed analysis by means descriptive statistics, analysis of variance and multivariate analysis, all of them aiming to study the individual sensory characteristics of quality of the coffee beverage from the “Matas de Minas” region. Path coefficient analysis also was carried out for the partition of the phenotypic correlation coefficients into measures of direct and indirect effects, in order to determine the individual sensory characteristics that played a major role in the beverage final score. The results demonstrate that it is not possible to conclusively establish the differences among coffees evaluated with basis on varieties and environmental factors previously cited. It can be concluded that it is not recommended to associate the quality of coffee only to a specific factor whether from the environment or being it a specific of the culture of coffee. However, the cafes that were evaluated had intrinsic quality, which were derived from the specific characteristics of the “Matas de Minas” region where they were grown.

关键词： Top Quality Coffee Mountain Coffee Coffee Cherry

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The Role of Nitrogen Fertilizers in sugarcane Root Biomass under Field Conditions

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Agricultural sciences 2014年第14期5卷 1527-1538页

作者： rafael Otto Henrique Coutinho Junqueira Franco Carlos Eduardo Faroni André Cesar Vitti Emídio Cantidio Almeida de Oliveira Renata Alcarde sermarini Paulo Cesar Ocheuze Trivelin department of soil science Luiz de Queiroz College of Agriculture University of Sao Paulo Piracicaba Brazil Brazilian Bioethanol science and Technology Laboratory (CTBE) Rua Giuseppe Maximo Scolfaro Campinas Brazil sugarcane Research Center (CTC) Piracicaba Brazil sao Paulo Agency of Agribusiness and Technology (APTA) Piracicaba Brazil Federal Rural University of Pernambuco (UFRPE) Recife Brazil department of Exact sciences Luiz de Queiroz College of Agriculture University of Sao Paulo Piracicaba Brazil Center for Nuclear Energy in Agriculture University of Sao Paulo Piracicaba Brazil

sugarcane is used worldwide for sugar, ethanol and energy production. In Brazil, the shift from burned to unburned harvest systems resulted in increases in nitrogen fertilization rates, which can impact root architecture and biomass. The expectation is also an increase in sugarcane biomass. The study hypothesized that high N rates applied to sugarcane fields increases root growth and N stored in roots, promoting higher biomass and N accumulated in shoots. Two experiments were set up in southeastern Brazil, on a Typic Kandiudox (TK) and Rhodic Eutrudox (RE). Four treatments were studied 1) N application in the plant-cane (0 and 120 kg·ha-1 N) and 2) N application in the ratoon (0 and 150 kg·ha-1 N). The shoot biomass and the root density (by the core method up to 0.6 m) were evaluated over the first ratoon crop cycle, and the N content in those compartments was also examined. There was no carry over effect on N applied at planting in root and shoot biomass in the ratoon crop cycle. At the RE site, the ratoon N fertilization increased root density in the superficial soil layer (0 - 0.2 m) and close to the plants (ss, and biomass and N accumulated in shoot was limited in both sites. Increasing N rates in unburned sugarcane fields do not consistently increases root and shoot biomass under Brazilian field conditions.

关键词： Root system Ratoon shoot Root: shoot Ratio

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Use of radiation in strains of saccharomyces cerevisiae:A new technique for industrial applications

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Advances in Bioscience and Biotechnology 2013年第3期4卷 346-351页

作者： rafael O.Andrette Joao D.T.Arruda-Neto Thiago O.Basso Luiz Carlos Basso Erika Cavalcante-silva Maria C.Bittencourt-Oliveira Godofredo C.Genofre Physics Institute University of Sao PauloSao PauloBrazil CEPEsq/UniItalo-Italy-Brazilian University Center Sao PauloBrazil Inter-Unities Graduation Program on Biotechnology University of Sao PauloSao PauloBrazil department of Biological sciences Luiz de Queiroz College of Agriculture/ESALQUniversity of Sao PauloPiracicabaBrazil Laboratory of Cyanobacteria ESALQUniversity of Sao PauloSao PauloBrazil Institute of Biosciences University of Sao PauloSao PauloBrazil

During the industrial fermentation process in the production of fuel ethanol, yeasts are subject to several stressing conditions. The survival and the permanence of strains introduced in the process correlate with the capability of these yeasts in resisting to physical and chemical stresses, as well as their recovering ability to compete with contaminating micro-organisms commonly present in this industrial process. We aim at the selection of saccharomyces cere visiae strains having this capability and ability. In this sense, cultivations of strains with industrial interest were irradiated with gammas ray at a wide dose interval. Growing curves for the strains were analyzed by means of their relative growth, a new concept here introduced, which allows a better understanding of the growing and recovering processes following radiative stress. It was found that gamma radiation could be used as an alternative method to quantify growing capabilities of s. cerevisiae strains under stressing conditions. It was also shown that this radiological method could be utilized as an additional procedure to select best robust industrial strains. This radiological method simplifies traditional analysis of strain viability, by avoiding the great number of necessary and consecutive fermentation assays.

关键词： saccharomyces cerevisiae Industrial Yeast Gamma Radiation Evaluation Method Industrial Fermentation Ethanol

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