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Elite,transformable haploid inducers in maize

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The Crop Journal 2024年第1期12卷 314-319页

作者： Brent Delzer Dawei Liang David Szwerdszarf Isadora Rodriguez Gonzalo Mardones Sivamani Elumalai Francine Johnson Samson Nalapalli rachel Egger Erin Burch Kerry Meier Juan Wei Xiujuan Zhang Huaping Gui Huaibing Jin Huan Guo Kun Yu Yubo Liu Becky Breitinger Ana Poets Jason Nichols Wan shi David Skibbe Qiudeng Que Timothy Kelliher Seeds Development Syngenta SeedsJanesvilleWI 53546USA Seeds Research Syngenta Biotechnology China Co.Ltd.Beijing 102206China Centro de Investigación de Cultivos Arica Syngenta R&DArica 1000000Chile Syngenta S.A. Proyecto la SoledadGraneros 2880000Chile Seeds Research Syngenta Crop Protection LLCRTPNC 27709USA Seeds Development Syngenta SeedsSlaterIA 50244USA

The introduction of alleles into commercial crop breeding pipelines is both time consuming and *** technologies that are disrupting traditional breeding processes are doubled haploid(DH)breeding and genome editing(GE).Recently,these techniques were combined into a GE trait delivery system called HI-Edit(Haploid Inducer-Edit).In HI-Edit,the pollen of a haploid inducer line is reprogrammed to deliver GE traits to any variety,obviating recurrent *** HI-Edit to operate at scale,an efficient transformable HI line is needed,but most maize varieties are recalcitrant to transformation,and haploid inducers are especially difficult to transform given their aberrant reproductive *** marker assisted selection and a three-tiered testing scheme,we report the development of new Iodent and Stiff Stalk maize germplasm that are transformable,have high haploid induction rates,and exhibit a robust,genetically-dominant anthocyanin native trait that may be used for rapid haploid *** show that transformation of these elite‘‘HI-Edit”lines is enhanced using the BABYBOOM and WUSCHEL morphogenetic ***,we evaluate the HI-Edit performance of one of the lines against both Stiff Stalk and non-Stiff Stalk *** strategy and results of this study should facilitate the development of commercially scalable HI-Edit systems in diverse crops.

关键词： Zea mays L Doubled haploids Transformation Genome editing QTL

Constitutive expression of feedback-insensitive cystathionine γ-synthase increases methionine levels in soybean leaves and seeds

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Journal of Integrative Agriculture 2018年第1期17卷 54-62页

作者： YU Yang HOU Wen-sheng Yael Hacham SUN shi WU Cun-xiang Ifat Matityahu SONG shi-kui rachel Amir HAN Tian-fu Key Laboratory of Soybean Biology (Beijing) Ministry of Agriculture/Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China Laboratory of Plant Science Migal Galilee Technology Center Kiryat Shmona 12100 Israel TeI-Hai College Upper Galilee 11016 Israel

Soybean （Glycine max （L.） Merr.） is a major crop that provides plant-origin protein and oil for humans and livestock. Al- though the soybean vegetative tissues and seeds provide a major source of high-quality protein, they suffer from low con- centration of an essential sulfur-containing amino acid, methionine, which significantly limits their nutritional quality. The level of methionine is mainly controlled by the first unique enzyme of methionine synthesis, cystathione y-synthase （CGS）. Aiming to elevate methionine level in vegetative tissues and seeds, we constitutively over-expressed a feedback-insensitive Arabidopsis CGS （AtD-CGS） in soybean cultivars, Zigongdongdou （ZD） and Jilinxiaoli 1 （JX）. The levels of soluble methionine increased remarkably in leaves of transgenic soybeans compared to wild-type plants （6.6- and 7.3-fold in two transgenic ZD lines, and 3.7-fold in one transgenic JX line）. Furthermore, the total methionine contents were significantly increased in seeds of the transgenic ZD lines （1.5- to 4.8-fold increase） and the transgenic JX lines （1.3- to 2.3-fold increase） than in the wild type. The protein contents of the transgenic soybean seeds were significantly elevated compared to the wild type, suggesting that the scarcity of methionine in soybeans may limit protein accumulation in soybean seeds. The increased protein content did not alter the profile of major storage proteins in the seeds. Generally, this study provides a promising strategy to increase the levels of methionine and protein in soybean through the breeding programs.

关键词： soybean (Glycine max (L.) Merr.) constitutive expression cystathionine y-synthase (CGS) methionine contentstorage protein

Recent advances in biodegradation controls over Mg alloys for bone fracture management: A review

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Journal of Materials Science & Technology 2019年第4期35卷 535-544页

作者： Ming-shi Song Rong-Chang Zeng Yun-Fei Ding rachel W.Li Mark Easton Ivan Cole Nick Birbilis Xiao-Bo Chen School of Engineering RMIT University Department of Materials Science and Engineering Monash University College of Materials Science and Engineering Shandong University of Science and Technology School of Mechanical Engineering Huaihai Institute of Technology The Medical School The Australian National University

Magnesium(Mg) alloys possess comparable physical and mechanical properties to bone, making them an outstanding candidate of implant materials for bone fracture treatment. In addition to the excellent biocompatibility, and bioactivity, the engagement of Mg alloys is key for a number of biological functionalities in the human body. The unique biodegradation nature of Mg alloy implants implies that it may not require a secondary removal procedure when the expected supporting tasks accomplish, as they may simply and safely "disappear" over time. Nonetheless, the demonstrated drawback of potentially rapid degradation, is an issue that must be addressed appropriately for Mg implants and is consequently given unique attention in this review article. Herein, the critical criteria and the state-of-the-art strategies for controlling the degradation process of Mg alloys are reported. Furthermore, future developments of biodegradable Mg and its alloys systems with satisfactory specifications for clinical trials and deployment,are discussed. This review aims to provide information to materials scientists and clinical practitioners in the context of developing practical biodegradable Mg alloys.

关键词： Magnesium alloys Biodegradation Corrosion mechanisms Cytocompatibility

Cataclysmic variables based on the stellar spectral survey LAMOST DR3

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Research in Astronomy and Astrophysics 2018年第6期18卷 67-88页

作者： Xianming L.Han Li-Yun Zhang Jian-Rong shi Qing-Feng Pi Hong-Peng Lu Li-Bo Zhao rachel K.Terheide Lin-Yang Jiang College of Physics/Department of Physics and Astronomy & Guizhou Provincial Key Laboratory of Public Big Data Guizhou University Dept.of Physics and Astronomy Butler University Key Laboratory for the Structure and Evolution of Celestial Objects Chinese Academy of Sciences National Astronomical Observatories Chinese Academy of Sciences Department of Astronomy Beijing Normal University

Big data in the form of stellar spectra from the spectroscopic survey associated with the Large Sky Area Multi-object Fiber Spectroscopic Telescope(LAMOST) are important for studying properties of cataclysmic variables(CVs). By cross matching the catalogs of CVs compiled with LAMOST DR3,acquired from October 2011 to July 2015, we obtained the first spectroscopic catalog for CVs observed by LAMOST with high signal to noise ratio, above 8. By integrating line profiles, their equivalent widths(EWs) of the Hα, Hβ, Hγ and Hδ, as well as He I 5876 and 6678 A? lines, were calculated. There were74 stellar spectra from 48 known CVs and three spectra from three new CV candidates. At the same time, we also collected their previously published EWs. Thirty-three objects had repeated spectra and30 stars showed spectral variability in the Hα line. Moreover, we carried out photometric follow-up studies for five CVs(UU Aqr, TT Tri, PX And, BP Lyn and RW Tri). We obtained nine new light curves and revised their linear ephemerides. For RW Tri, there is a possible oscillation with an amplitude of0.0031(2) days and a period of 47.6±0.4 years, which might be caused by a third body(brown dwarf)or magnetic activity cycle.

关键词： stars: cataclysmic variables stars: spectroscopic stars: variable

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紧急疏散条件下交通控制设施选址研究

交通运输系统工程与信息 2011年第3期11卷 138-143页

作者：张雄飞史其信 HE rachel BAN Jeff 清华大学交通研究所北京100084 美国圣克拉拉大学土木工程系加州95053 美国伦斯勒理工学院土木与环境工程系纽约州12180

针对地震、飓风等自然或者人为灾害条件下,大规模路网疏散的交通管理问题进行了研究.由于紧急疏散条件下,交通控制设施和管理人员有限,很难覆盖交通网络中的所有交通节点,因此,识别出路网中重要的交通节点并加以控制,以达到最优的系统... 详细信息

针对地震、飓风等自然或者人为灾害条件下,大规模路网疏散的交通管理问题进行了研究.由于紧急疏散条件下,交通控制设施和管理人员有限,很难覆盖交通网络中的所有交通节点,因此,识别出路网中重要的交通节点并加以控制,以达到最优的系统状态显得尤为重要.为了解决这一迫切的既理论而又实际的问题,本研究提出了一个混合整数非线性规划(Mixed Integer NonLinear Programming,MINLP)模型,以确定最优的交通控制节点选址,并同时确定这些控制节点的最佳交通控制策略,以最大限度地减少整个交通系统成本.数值算例表明了模型的适用性和合理性,同时得出结论,只要识别出重要的交通节点并加以控制,系统也能接近所有交通节点都控制时的理想最优状态.

关键词：交通工程用户均衡系统最优非线性规划紧急疏散

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齐口裂腹鱼幼鱼疲劳后游泳特性恢复状况研究

水生生物学报 2013年第6期37卷 993-998页

作者：蔡露刘国勇 rachel Taupier 涂志英石小涛黄应平三峡大学三峡库区生态环境教育部工程研究中心宜昌443002 三峡地区地质灾害与生态环境湖北省协同创新中心宜昌443002 School of Natural Sciences and Mathematics Ferrum College Ferrum VA 24088 USA

以中国地质公园(神农架)野生动物繁育基地提供的齐口裂腹鱼(Schizothorax prenanti)幼鱼(体长为14.0—17.5 cm,体重为39.6—65.6 g)为研究对象,利用自制鱼类游泳特性(Swimming performance)研究装置,运用递增流速法(Stepped velocity te... 详细信息

以中国地质公园(神农架)野生动物繁育基地提供的齐口裂腹鱼(Schizothorax prenanti)幼鱼(体长为14.0—17.5 cm,体重为39.6—65.6 g)为研究对象,利用自制鱼类游泳特性(Swimming performance)研究装置,运用递增流速法(Stepped velocity test),研究了鱼类疲劳后20d和40d的游泳特性恢复状况。结果表明:(1)在20d内齐口裂腹鱼临界游泳速度(Critical swimming speed,Ucrit)相比于初次测试(20d前)有所降低,20d内的恢复状况较差;而在40d后,临界游泳速度已经基本恢复到最初状况,40d内的恢复状况较好。(2)疲劳后鱼类游泳效率显著下降,并且在40d后无法恢复正常。虽然40d后速度指数低于20d后速度指数,40d后游泳效率高于20d后游泳效率,但是相对于初次测试,40d后游泳效率恢复效果并不明显。(3)齐口裂腹鱼在疲劳后40d内,恢复能力(Recovery capability)无法恢复到正常状况,但由于3次测试中疲劳后过量耗氧(Excess post-exercise oxygen cost,EPOC)波动并不大,说明疲劳对其恢复能力影响并不大。(4)20d后和40d后无氧呼吸时间均较第一次测试时提前,说明疲劳损伤对其有氧呼吸能力产生了持续影响。(5)疲劳对鱼类生态行为(Ecological behavior)影响不明显。研究有利于为鱼道建设提供基础资料,对生物多样性保护具有深远意义。

关键词：齐口裂腹鱼游泳特性疲劳恢复状况临界游泳速度生态行为

Isolated sacral injuries: Postoperative length of stay, complications, and readmission

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World Journal of Orthopedics 2015年第8期6卷 629-635页

作者： Vasanth Sathiyakumar Hanyuan shi rachel V Thakore Young M Lee David Joyce Jesse Ehrenfeld William T Obremskey Manish K Sethi the Vanderbilt Orthopaedic Institute Center for Health Policy Medical Center East

AIM: To investigate inpatient length of stay(LOS), complication rates, and readmission rates for sacral fracture patients based on operative ***: All patients who presented to a large tertiary care center with isolated sacral fractures in an 11-year period were included in a retrospective chart review. Operative approach(open reduction internal fixation vs percutaneous) was noted, as well as age, gender, race, and American Society of Anesthesiologists' score. Complications included infection, nonunion and malunion, deep venous thrombosis, and hardware problems; 90-d readmissions were broken down into infection, surgical revision of the sacral fracture, and medical complications. LOS was collected for the initial admission and readmission visits if applicable. Fisher's exact and non-parametric t-tests(Mann-Whitney U tests) were employed to compare LOS, complications, and readmissions between open and percutaneous ***: Ninety-four patients with isolated sacral fractures were identified: 31(30.4%) who underwentopen reduction and internal fixation(ORIF) vs 63(67.0%) who underwent percutaneous fixation. There was a significant difference in LOS based on operative approach: 9.1 d for ORIF patients vs 6.1 d for percutaneous patients(P = 0.043), amounting to a difference in cost of $13590. Ten patients in the study developed complications, with no significant difference in complication rates or reasons for complications between the two groups(19.4% for ORIF patients vs 6.3% for percutaneous patients). Eight patients were readmitted, with no significant difference in readmission rates or reasons for readmission between the two groups(9.5% percutaneous vs 6.5% ORIF).CONCLUSION: There is a significant difference in LOS based on operative approach for sacral fracture patients. Given similar complications and readmission rates, we recommend a percutaneous approach.

关键词： Sacral fractures Open reduction and internal fixation Percutaneous complications Readmissions Length of stay

Reduced cardiac output is associated with decreased mitochondrial efficiency in the non-ischemic ventricular wall of the acute myocardial-infarcted dog

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Cell Research 2006年第3期16卷 297-305页

作者： Zakaria A Almsherqi Malgorzata B Slocinska Yuru Deng Department of Physiology,National University of Singapore,Block MD9,2 Medical Drive,Singapore 117597,Singapore Craig S McLachlan Peter Mossop Department of Medical Imaging,St Vincents Hospital,Melbourne,Fitzroy VIC 3065,Australia Francis E Sluse rachel Navet Laboratory of Bioenergetics,Institute of Chemistry B6,University of Liége,Start Tilman,B-4000 Liége,Belgium Nikolai Kocherginsky Iouri Kostetski Biophysics Laboratory,Division of Bioengineering,National University of Singapore,Singapore 117576,Singapore Dong-Yun shi Shan-Lin Liu Department of Biochemistry,Shanghai Medical College of Fudan University,Free Radical Regulation Research Center of Fudan University,Shanghai 200032,China Department of Physiology National University of Singapore Singapore Singapore Department of Medical Imaging St Vincents Hospital Melbourne Australia Laboratory of Bioenergetics Institute of Chemistry B6 University of Liége Start Tilman Liége Belgium Division of Bioengineering Biophysics Laboratory National University of Singapore Singapore Singapore Department of Biochemistry Shanghai Medical College of Fudan University Free Radical Regulation Research Center of Fudan University Shanghai China

Cardiogenic shock is the leading cause of death among patients hospitalized with acute myocardial infarction (MI).Understanding the mechanisms for acute pump failure is therefore *** aim of this study is to examine in anacute MI dog model whether mitochondrial bio-energetic function within non-ischemic wall regions are associated withpump *** MI was produced in dogs via ligation of left anterior descending (LAD) coronary artery,thatresulted in an infract size of about 30% of the left ventricular *** of hemodynamic status,mitochondrialfunction,free radical production and mitochondrial uncoupling protein 3 (UCP3) expression were determined over 24h *** measurements revealed a>50% reduction in cardiac output at 24 h post infarction when com-pared to *** samples were obtained from the posterior non-ischemic wall during acute ***/Oratios for isolated mitochondria from non-ischemic myocardium at 6 h and 24 h were decreased when compared to theADP/O ratios within the same samples with and without palmitic acid (PA).GTP inhibition of (PA)-stimulated state 4respiration in isolated mitochondria from the non-ischemic wall increased by 7% and 33% at 6 h and 24 h post-infarctionrespectively when compared to sham and pre-infarction *** would suggest that the mitochondria are uncoupledand this is supported by an associated increase in UCP3 expression observed on western blots from these same *** samples from the coronary sinus measured by electron paramagnetic resonance (EPR) methods showedan increase in reactive oxygen species (ROS) over baseline at 6 h and 24 h *** conclusion,mitochondrialbio-energetic ADP/O ratios as a result of acute infarction are abnormal within the non-ischemic *** ap-pear to be energetically uncoupled and this is associated with declining pump *** radical production may beassociated with the induction of uncoupling prot

关键词： mitochondria uncoupling proteins bioenergetics infarction cardiogenic shock non-ischemic ventricle

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牡蛎礁碳源–汇功能研究进展与展望

渔业科学进展 2022年第5期43卷 115-125页

作者：奉杰张涛马培振白涛徐江玲王海艳宋浩赵伟赵亮杨美洁胡志周骢石璞胡朋朋李海州中国科学院海洋生态与环境科学重点实验室山东青岛266071 中国科学院海洋生物分类与系统演化实验室山东青岛266071 青岛海洋科学与技术试点国家实验室海洋生态与环境科学功能实验室山东青岛266237 中国科学院海洋大科学研究中心山东青岛266071 中国科学院海洋牧场工程实验室山东青岛266071 国家海洋局北海预报中心山东青岛266033 山东富瀚海洋科技有限公司山东海阳265116

针对当前全球气候变暖趋势,中国提出“双碳”目标,体现了我国主动承担应对全球气候变化责任的大国担当。海洋在实现碳中和目标中具有重要作用。牡蛎礁作为全球海岸带典型生态系统,具有巨大碳储量和强大固碳能力。牡蛎礁在生物钙化、呼... 详细信息

针对当前全球气候变暖趋势,中国提出“双碳”目标,体现了我国主动承担应对全球气候变化责任的大国担当。海洋在实现碳中和目标中具有重要作用。牡蛎礁作为全球海岸带典型生态系统,具有巨大碳储量和强大固碳能力。牡蛎礁在生物钙化、呼吸作用等过程中向大气释放二氧化碳,但在生物合成、沉积作用等过程中却可以埋藏大量碳。目前,全球牡蛎礁是大气碳的源还是汇尚不明确。为探究牡蛎礁碳源–汇功能,本文综述了牡蛎礁碳源–汇功能研究现状,分析了影响牡蛎礁碳源–汇功能的关键生态过程,探讨了在不同环境条件下牡蛎礁碳源–汇特征。研究表明,牡蛎礁不仅可以成为大气碳的汇,还可以提高盐沼植被、海藻、海洋动物等生物的碳汇功能。未来应尽快开展牡蛎礁碳汇功能评估技术等研究,形成以提高牡蛎礁碳汇为目的的牡蛎礁保护与修复技术,提升我国海洋生态系统碳汇能力。

关键词：牡蛎礁碳源–汇功能储碳气候变化保护与修复