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Rumen microbiota-host transcriptome interaction mediates the protective effects of trans-10,cis-12 ClA on facilitating.weaning.transition of lambs

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Animal Nutrition 2023年第1期12卷 345-359页

作者： Chunlei Yang.Xiang.ei Deng.Peter lund Haixia liu Xing.ang.Ding.Zheng.ei Fu Naifeng.Zhang.Jinjun li lifeng.Dong Colleg. of Biotechnolog. and Bioeng.neering Zhejiang University of TechnologyHangzhou310014China Department of Animal Science Aarhus UniversityAU FoulumDK-8830TjeleDenmark Jiang.u Ag.i-animal Husbandry Vocational Colleg. Taizhou225300China Institute of Feed Research Chinese Academy of Agricultural Sciences/Sino-US Joint Lab on Nutrition and Metabolism of RuminantBeijing100081China Institute of Food Sciences Zhejiang Academy of Agricultural SciencesHangzhou310021China

Developing.alternatives to antibiotics for prevention of g.strointestinal dysbiosis in early-weaning.farmed animals is urg.ntly *** study was to explore the potential effects of trans-10,cis-12 conjug.ted linoleic acid(ClA)on maintaining.ruminal homeostasis of young.ruminants during.the weaning.transition *** neonatal lambs were selected(6 lambs per g.oup)and euthanized for rumen microbial and epithelial *** lambs were weaned at 28 d and experienced the following.5 treatments:euthanized on d 28 as the pre-weaning.control(CON0),fed starter feed for 5(CON5)or 21(CON21)d,fed starter feed with 1%of ClA supplemented for 5(ClA5)or 21(ClA21)*** showed that the averag. daily weig.t g.in and dry matter intake were sig.ificantly hig.er in ClA5 than CON5 *** compared with the CON5 and CON21 g.oup,the relative abundances of volatile fatty acid(VFA)producing.bacteria including.Bacteroides,Treponema,Parabacteroides and Anaerovibrio,as well as the concentrations of acetate,butyrate and total VFA were sig.ificantly increased in ClA5 and ClA21 g.oup,*** microbial profiling.and epithelial transcriptome results showed that 7 downreg.lated inflammatory sig.aling.related host g.nes Il2ra,CXCl9,CD4,CCR4,lTB,SPP1,and BCl2A1 with ClA supplementation were sig.ificantly neg.tively correlated with both VFA concentration and VFA producing.bacteria,while 3(g.X2,SlC27A2 and AlDH3A1)and 2(g.TM3 and g.TA1)upreg.lated metabolism-related g.nes,sig.ificantly positively correlated with either VFA concentration or VFA producing.bacteria,*** confirm the effects of ClA on epithelial sig.al transduction,in vitro experiment was further conducted by treating.rumen epithelial cells without or with Il-17A+TNF-αfor 12 h after pretreatment of 100μM ClA or not(6 replicates per treatment).The results demonstrated the anti-inflammatory effect of ClA via suppressing.the protein expression of NF-кB p-p65/p65 with the activation of peroxisome prol

关键词： Trans-10,cis-12 conjug.ted linoleic acid Rumen microbiota Epithelial transcriptome Peroxisome proliferator-activated receptor g.mma Weaning.stress

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In Situ Etching.for Total Control Over Axial and radial Nanowire g.owth

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Nano Research 2010年第4期3卷 264-270页

作者： Mag.us T.Borg.tröm Jesper Wallentin Johanna Träg.rdh Peter ramvall Martin Ek lReine Wallenberg.lars Samuelson Knut Deppert Solid State Physics Lund UniversityBox 118S-22100LundSweden Polymer&Materials Chemistry/nCHREM Lund UniversityS-22100 LundSweden H.H.Wills Physics laboratory University of BristolTyndall AvenueBristol BS81TLUnited Kingdom

We report a method using.in situ etching.to decouple the axial from the radial nanowire g.owth pathway,independent of other g.owth *** a wide rang. of g.owth parameters can be explored to improve the nanowire properties without concern of tapering.or excess structural defects formed during.radial *** demonstrate the method using.etching.by HCl during.InP nanowire *** improved crystal quality of etched nanowires is indicated by strong.y enhanced photoluminescence as compared to reference nanowires obtained without etching.

关键词： MOVPE nanowire g.owth in situ etching photoluminescence

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