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Repeated-batch Cultivation of Encapsulated Monascus purpureus by Polyelectrolyte Complex for Natural Pigment Production

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Chinese Journal of Chemical Engineering 2010年第6期18卷 1013-1017页

作者： LIU Jiefeng ren yiran YAO Shanjing Department of Chemical and Biological Engineering Zhejiang University Hangzhou 310027 China Department of Biotechnology and Food Engineering Maoming College Maoming 525000 China

In general,productions of natural pigment in submerged microorganism culture were much less than that in solid-state fermentation,because the solid-state culture can provide a support carrier for the mycelium. To improve natural pigment production,the cultivation of Monascus purpureus in submerged encapsulated cell was investigated. Monascus purpureus immobilized in polyelectrolyte complex(PEC) microcapsules,which were pre-pared by sodium cellulose sulphate(NaCS) and poly-dimethyl-diallyl-ammonium chloride(PDMDAAC),was a good substitute for submerged cell culture because it mimicked the solid-state environment. The repeated-batch process with encapsulated cells was studied in flasks and a bubble column. The results indicated that the bubble column was more suitable for the encapsulation culture than the shaking flasks because of its good mass transfer performance and minor shear stress on cells. Owing to the protection of the microcapsule's membrane,Monascus purpureus in microcapsules increased approximately three times over that in free cell culture with negligible cell leakage to the medium. The pigment production in the bubble column finally reached 3.82(OD500) ,which was two times higher than in free cell culture. In addition,the duration of each batch was shortened to 15% of that in free cell culture.

关键词： microencapsulation Monascus purpureus natural pigment polyelectrolyte complex NaCS-PDMDAAC capsule cell immobilization

A gated strategy stabilizes room-temperature phosphorescence

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聚集体(英文)

聚集体(英文) 2023年第4期4卷 224-229页

作者： Kaizhi Gu Zhengong Meng Xing Wang Liu Yue Wu Xin Qi yiran ren Zhen-Qiang Yu Ben Zhong Tang College of Chemistry and Environmental Engineering Shenzhen UniversityShenzhenChina Institute for Advanced Study Central South UniversityChangshaChina School of Science and Engineering Shenzhen Institute of Aggregate Science and TechnologyThe Chinese University of Hong KongShenzhenChina

Room-temperature phosphorescence(RTP)of purely organic materials is easily quenched with unexpected purposes because the excited triplet state is extremely susceptible to external *** to stabilize the RTP property of purely organic luminogens is still challenging and considered as the bottleneck in the further advancement of the bottom-up ***,we describe a gated strategy that can effectively harness RTP by employing complexation/dissociation with *** to the order-disorder transition orientation of intermolecular packing,the RTP of organic molecules 2,4,6-tris(4′-bromo-[1,1′-biphenyl]-4-yl)-1,3,5-triazine(Br-TRZ)will easily vanish upon mechanical ***,by enhancing its intramolec-ular charge transfer effect,the protonated Br-TRZ stubbornly possesses an obvious RTP under external grinding,whatever in the ordered or disordered intermolec-ular arrangement ***,the“Lock”gate of RTP was achieved in the protonated Br-TRZ *** with theoretical calculation analy-sis,the enhanced charge transfer effect can narrow the energy gap singlet-tripletsignificantly,and stabilize the RTP property of triazine derivative ***-thermore,the locked RTP can be tuned via proton and counterions repeatedly and show excellent *** gated RTP concept provides an effective strategy for stabilizing the RTP emission of purely organic systems.

关键词： gated effect grinding molecular packing protonation room-temperature phosphorescence

Microscopic and spectroscopic analysis of atmospheric iron-containing single particles in Lhasa,Tibet

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Journal of Environmental Sciences 2024年第7期141卷 40-50页

作者： Junyu Chen Daobin Zhang yiran Fu Jinhu Wang Xiaomei Cui Ci-ren Qu-zong Qiangying Zhang Chan Jin Bu Duo School of Ecology and Environment Tibet UniversityLhasa 850000China Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China Institute of Tibetan Plateau Research Chinese Academy of SciencesBeijing 100101China

The Tibetan Plateau,known as the“Third Pole”,is currently in a state of perturbation caused by intensified human *** this study,56 samples were obtained at the five sampling sites in typical area of Lhasa city and their physical and chemical properties were investigated by TEM/EDS,STXM,and NEXAFS *** careful examination of 3387single particles,the results showed that Fe should be one of the most frequent metal *** Fe-containing single particles in irregular shape and micrometer size was about7.8%and might be mainly from local ***,the Fe was located on the subsurface of single particles and might be existed in the form of iron ***,the core-shell structure of iron-containing particles were about 38.8%and might be present as single-,dual-or triple-core shell structure and multi-core shell structure with the Fe/Si ratios of 17.5,10.5,2.9 and 1.2,***,iron and manganese were found to coexist with identical distributions in the single particles,which might induce a synergistic effect between iron and manganese in catalytic ***,the solid spherical structure of Fe-containing particles without an external layer were about 53.4%.The elements of Fe and Mn were co-existed,and might be presented as iron oxide-manganese oxide-silica ***,the ferrous and ferric forms of iron might be *** information can be valuable in expanding our understanding of Fe-containing particles in the Tibetan Plateau atmosphere.

关键词： Fe-containing single particle Irregular shape Core-shell structure Solid spherical structure Transmission electron microscopy Synchrotron X-ray microscopy

Regulating photocatalytic overall water splitting of ferroelectric heterostructures by size effect

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Nano Research 2024年第9期17卷 8000-8006页

作者： Zixing Ye Daifu Yu Ruian Zhang Fei Qin yiran Sun Jie Huang Zhanqi Zhou He Tian Gaorong Han Zhaohui ren Gang Liu State Key Laboratory of Silicon and Advanced Semiconductor Materials School of Materials Science and EngineeringZhejiang UniversityHangzhou 310027China Shenyang National Laboratory for Materials Science Institute of Metal ResearchChinese Academy of SciencesShenyang 110016China Center of Electron Microscopy School of Materials Science and EngineeringZhejiang UniversityHangzhou 310027China

In the past decade,ferroelectric materials have been intensively explored as promising *** intriguing ability of ferroelectrics is to directly sperate the photogenerated electrons and holes,which is believed to arise from a spontaneous *** how polarization affects the photocatalytic performance is vital to design high-efficiency *** this work,we report a size effect of ferroelectric polarization on regulating the photocatalytic overall water splitting of SrTiO_(3)/PbTiO_(3)nanoplate heterostructures for the first *** was realized hydrothermally by controlling the thickness and thus spontaneous polarization strength of single-crystal and single-domain PbTiO_(3)nanoplates,which served as the substrate for selective heteroepitaxial growth of SrTiO_(3).An enhancement of 22 times in the photocatalytic overall water splitting performance of the heterostructures has been achieved when the average thickness of the nanoplate increases from 30 to 107 nm.A combined experimental investigation revealed that the incompletely compensated depolarization filed is the dominated driving force for the photogenerated carrier separation within heterostructures,and its increase with the thickness of the nanoplates accounts for the enhancement of photocatalytic ***,the concentration of oxygen vacancies for negative polarization compensation has been found to grow as the thickness of the nanoplates increases,which promotes oxygen evolution reaction and reduces the stoichiometric ratio of H_(2)/O_(2).These findings may provide the opportunity to design and develop high-efficiency ferroelectric photocatalysts.

关键词： single-domain nanoplates size effect ferroelectric polarization screening photocatalytic overall water splitting

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苹果MdRGL3a基因的功能分析

园艺学报 2017年第6期44卷 1021-1028页

作者：任怡然赵强由春香郝玉金山东农业大学园艺科学与工程学院作物生物学国家重点实验室国家苹果工程技术研究中心山东泰安271018

为进一步揭示苹果(Malus×domestica)中GA信号转导机制以及MdDELLAs的生理功能,以‘嘎拉’苹果为试材,克隆基因MdRGL3a(GenBank:DQ007887),并通过在拟南芥中过量表达,分析该基因的功能。qRT-PCR结果表明,MdRGL3a在苹果不同组织中都有表... 详细信息

为进一步揭示苹果(Malus×domestica)中GA信号转导机制以及MdDELLAs的生理功能,以‘嘎拉’苹果为试材,克隆基因MdRGL3a(GenBank:DQ007887),并通过在拟南芥中过量表达,分析该基因的功能。qRT-PCR结果表明,MdRGL3a在苹果不同组织中都有表达,其中在花和果实中表达量较高。在拟南芥中异位表达MdRGL3a,表现为根长和下胚轴变短,种子萌发较慢,叶片变小,植株矮化。过表达MdRGL3a拟南芥植株对赤霉素介导的生长反应敏感性降低,证明MdRGL3a是GA信号途径中的一个负调控因子。

关键词：苹果 MdRGL3a DELLA蛋白表达分析功能鉴定

学习共同体视角下高校学习型寝室的创建与实现路径

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科教文汇 2021年第4期 49-50页

作者：任怡然黑龙江大学黑龙江哈尔滨150080

寝室作为高校学生居住休息的场所,占据了大学生在校期间三分之一以上的时间,对青年学生有着重要的影响。在学习共同体视角下,只有充分发挥寝室团体与学生个体的主动性,才能逐渐形成完整的体系,建立起有效运行的机制。把学习共同体理论... 详细信息

寝室作为高校学生居住休息的场所,占据了大学生在校期间三分之一以上的时间,对青年学生有着重要的影响。在学习共同体视角下,只有充分发挥寝室团体与学生个体的主动性,才能逐渐形成完整的体系,建立起有效运行的机制。把学习共同体理论引入学习型寝室的建设中,能够挖掘寝室的学习功能,加强寝室文化建设,促进大学生的全面发展。

关键词：学习共同体学习型寝室高校校风建设

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地保:“胥吏之害”与阿Q精神胜利法的生成

中国现代文学论丛 2023年第4期18卷 187-210页

作者：孙伟任旖然暨南大学文学院

对于阿Q精神胜利法生成的研究,需要突破启蒙和革命等后设话语的束缚,重返阿Q所处的真实历史现场。阿Q三十年人生正处于中国剧烈变动转型期,社会下层豪民猾吏乘机而起称霸乡村,地保利用职务之便成为基层乡村的重要统治力量。地保所从属... 详细信息

对于阿Q精神胜利法生成的研究,需要突破启蒙和革命等后设话语的束缚,重返阿Q所处的真实历史现场。阿Q三十年人生正处于中国剧烈变动转型期,社会下层豪民猾吏乘机而起称霸乡村,地保利用职务之便成为基层乡村的重要统治力量。地保所从属的胥吏阶层是构成中国前现代社会基层治理体系的重要政治力量,不在行政编制内但掌握着管理基层社会的实质性权力,由于官僚体制的缺陷导致他们位卑权重、监管不当而以权谋私、欺压百姓。胥吏阶层之所以能在乡村肆意妄为,与皇、士、军三个群体有着极为密切的关联,皇权的下放造成胥吏阶层拥有制度保障的正当权力,劣绅的合谋致使传统乡村共同体的伦理关怀消失不见,军阀的暴力导致社会秩序的失序和混乱。以地保介入基层乡村权力架构为切入口,在地保所从属的胥吏阶层与皇、士、军三方势力的互动关系中开出阿Q精神胜利法生成的新思路。

关键词：《阿Q正传》精神胜利法地保胥吏

Unraveling a genetic roadmap for improved taste in the domesticated apple

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Molecular Plant 2021年第9期14卷 1454-1471页

作者： Liao Liao Weihan Zhang Bo Zhang Ting Fang Xiao-Fei Wang Yarning Cai Collins Ogutu Lei Gao Gang Chen Xiaoqing Nie Jinsheng Xu Quanyan Zhang yiran ren Jianqiang Yu Chukun Wang Cecilia H.Deng Baiquan Ma Beibei Zheng Chun-Xiang You Da-Gang Hu Richard Espley Kui Lin-Wang Jia-Long Yao Andrew C.Allan Awais Khan Schuyler S.Korban Zhangjun Fei Ray Ming Yu-Jin Hao Li Li Yuepeng Han CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture Hubei Hongshan LaboratoryWuhan Botanical GardenThe Innovative Academy of Seed DesignChinese Academy of SciencesWuhan 430074China Agricultural Bioinformatics Key Laboratory of Hubei Province College of InformaticsHuazhong Agricultural UniversityWuhan 430070China Center of Economic Botany Core Botanical GardensChinese Academy of SciencesWuhan 430074China University of Chinese Academy of Sciences 19A YuquanluBeijing 100049China College of Horticulture Science and Engineering Shandong Agricultural UniversityTai-An 271018China Sino-African Joint Research Center Chinese Academy of SciencesWuhan 430074China The New Zealand Institute for Plant&Food Research Limited AucklandNew Zealand School of Biological Sciences University of AucklandPrivate Bag 92019Auckland 1142New Zealand Plant Pathology and Plant-Microbe Biology Section Cornell UniversityGenevaNY 14456USA Department of Natural Resources and Environmental Sciences University of Illinois at Urbana-ChampaignUrbanaIL 61801USA Boyce Thompson Institute Cornell UniversityIthacaNY 14853USA Department of Plant Biology University of Illinois at Urbana-ChampaignUrbanaIL 61801USA

Although taste is an important aspect of fruit quality, an understanding of its genetic control remains elusive in apple and other fruit crops. In this study, we conducted genomic sequence analysis of 497 Malus accessions and revealed erosion of genetic diversity caused by apple breeding and possible independent domestication events of dessert and cider apples. Signatures of selection for fruit acidity and size, but not for fruit sugar content, were detected during the processes of both domestication and improvement. Furthermore, we found that single mutations in major genes affecting fruit taste, including Ma1, MdTDT, and MdSOT2, dramatically decrease malate, citrate, and sorbitol accumulation, respectively, and correspond to important domestication events. Interestingly, Ma1 was identified to have pleiotropic effects on both organic acid content and sugar:acid ratio, suggesting that it plays a vital role in determining fruit taste. Fruit taste is unlikely to have been negatively affected by linkage drag associated with selection for larger fruit that resulted from the pyramiding of multiple genes with minor effects on fruit size. Collectively, our study provides new insights into the genetic basis of fruit quality and its evolutionary roadmap during apple domestication, pinpointing several candidate genes for genetic manipulation of fruit taste in apple.

关键词： apple fruit taste organic acids soluble sugars fruit size domestication

城市生命体的历史与想象——论邱华栋《北京传》

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东吴学术 2022年第6期 94-99页

作者：唐诗人任旖然暨南大学文学院暨南大学文艺理论教研室

作家写城市传已成为一个重要的创作现象,邱华栋的《北京传》是其中的代表性文本。《北京传》的历史架构宏大,是对北京城历史的全景叙述,同时也填充了很多文学想象性内容,是作家个人的城市生活观察记录。邱华栋以非虚构式的文学笔法,创... 详细信息

作家写城市传已成为一个重要的创作现象,邱华栋的《北京传》是其中的代表性文本。《北京传》的历史架构宏大,是对北京城历史的全景叙述,同时也填充了很多文学想象性内容,是作家个人的城市生活观察记录。邱华栋以非虚构式的文学笔法,创造性地讲述了北京城的空间延展与中华文明发展变迁之间的内在关联,真正把一座城市写成了有辉煌历史也有当代活力的生命体。

关键词：邱华栋《北京传》城市生命体城市传