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Insect resistance management in Bacillus thuringiensis cotton by MGPS(multiple genes pyramiding and silencing)

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Journal of Cotton Research 2020年第4期3卷 311-323页

作者： ZAFAR Muhammad Mubashar RAZZAQ Abdul FAROOQ Muhammad Awais REHMAN Abdul FIRDOUS Hina SHAKEEL Amir MO Huijuan ren maozhi Institute of Cotton Research Chinese Academy of Agricultural SciencesAnyang 455000HenanChina Department of Plant Breeding and Genetics University of Agriculture FaisalabadFaisalabad 38000Pakistan Zhengzhou Research Base State Key Laboratory of Cotton BiologyZhengzhou UniversityZhengzhou 450000China Department of Plant Pathology University of Agriculture FaisalabadFaisalabadPakistan.

The introduction of Bacillus thuringiensis(Bt)cotton has reduced the burden of pests without harming the environment and human ***,the efficacy of Bt cotton has decreased due to field-evolved resistance in insect pests over *** this review,we have discussed various factors that facilitate the evolution of resistance in cotton ***,different strategies like pyramided cotton expressing two or more distinct Bt toxin genes,refuge strategy,releasing of sterile insects,and gene silencing by RNAi are being used to control insect *** cotton has shown resistance against different cotton *** multiple genes pyramiding and silencing(MGPS)approach has been proposed for the management of cotton *** genome information of cotton pests is necessary for the development of MGPS-based *** expression cassettes against various essential genes involved in defense,detoxification,digestion,and development of cotton pests will successfully obtain favorable agronomic characters for crop protection and *** MGPS involves the construction of transformable artificial chromosomes,that can express multiple distinct Bt toxins and RNAi to knockdown various essential target genes to control *** evolution of resistance in cotton pests will be delayed or blocked by the synergistic action of high dose of Bt toxins and RNAi as well as compliance of refuge requirement.

关键词： Bt cotton RNAi Multiple genes pyramiding and silencing(MGPS) Bt resistance

Genetic variability for yield and fiber related traits in genetically modified cotton

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Journal of Cotton Research 2021年第3期4卷 219-228页

作者： SAHAR Adeela ZAFAR Muhammad Mubashar RAZZAQ Abdul MANAN Abdul HAROON Muhammad SAJID Sunaina REHMAN Abdul MO Huijuan ASHRAF Muhammad ren maozhi SHAKEEL Amir YUAN Youlu Department of Plant Breeding and Genetics University of AgricultureFaisalabadPakistan Institute of Cotton Research Chinese Academy of Agricultural SciencesAnyang 455000China Institute of Molecular Biology and Biotechnology The University of Lahore-PakistanLahorePakistan National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhanChina Zhengzhou Research Base State Key Laboratory of Cotton BiologyZhengzhou UniversityZhengzhouChina.

Background:Cotton(Gossypium hirsutum L.)is grown for fiber and oil purposes in tropical and sub-tropical areas of the *** is the 4th largest producer of *** has a significant contribution in the GDP of ***,the present study was performed to assess the genetic variations and genetic diversity of yield and fiber quality traits in cotton and to analyze the associations present among ***:Analysis of variance exhibited significant variation for all studied traits except total number of nodes and the height to node *** phenotypic coefficient of variation was higher than the genotypic coefficient of variation for all studied *** height,monopodial branches,total number of bolls,lint index,seed index,and seed cotton yield displayed high heritabilities in a broad sense with maximum genetic *** analysis revealed that seed cotton yield had a significant positive association with plant height,the nu mber of monopodial branches,the nu mber of sympodia I branches,ginning outturn(GOT),the number of bolls,seed per boll,seed index,uniformity index,the number of sympodial branches,reflectance,and seed index at the genotypic level while a significant positive relationship was observed with plant height,the number of sympodial branches,boll number,and *** height,monopodial branches,GOT,boll weight,seeds per boll,and short fiber index exerted direct positive effects on seed cotton *** first 6 principal component analysis(PCs)out of the total fourteen PCs displayed eigenvalues(>1)and had maximum share to total variability(82.79%).The attributes that had maximum share to total divergence in eluded plant height,uniformity index,the nu mber of sympodial branches,seed per boll,GOT,seed cotton yield,and short fiber ***:The genotype AA-802,IUB-13,FH-159,FH-458,and CIM-595 were genetically diverse for most of the yield and fiber quality traits and could be utilized for the selection of better perform

关键词： Upland cotton Fiber trait Cotton yield Genetic variability Yield components

Study on Expression and Influencing Factor of Bt Insecticidal Protein in Transgenic Insect-resistant Cotton

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Study on Expression and Influencing Factor of Bt Insecticida...

Functional analysis of a reproductive organ predominant expressing promoter in cotton plants

中国作物学会2013年学术年会

作者： SUN Guoqing ZHANG Rui WANG Yuan ren maozhi MENG Zhigang ZHOU Tao GUO Sandui Biotechnology Research Institute National Key Facility of Crop Gene Resources and Genetic ImprovementChinese Academy of Agricultural Sciences

Expression of foreign gene in plants is affected by many factors,such as the insertion site,copy numbers,the external environment et *** applications,however,the popular topic is whether the expression of foreign genes can achieve the desired *** artificially modified foreign genes of CrylAb/Ac and CpTI were transferred to cotton that is the most important cash crop in China,and were commercialization and industrialization of

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Science China(Life Sciences) 2005年第5期48卷 452-459页

作者： ren maozhi1,3 , CHEN Quanjia2 , LI Li1, ZHANG Rui1 & GUO Sandui1 1. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China 2. Department of Agronomy, Xinjiang Agricultural University, Urumchi 830052, China 3. College of Life Science and Technology, Shanghai Jiao Tong University, Shanghai 200030, China 1. Biotechnology Research Institute Chinese Academy of Agricultural Sciences 100081 Beijing China 2. College of Life Science and Technology Shanghai Jiao Tong University 200030 Shanghai China 3. Department of Agronomy Xinjiang Agricultural University 830052 Urumchi China

Transgenic Bt insect-resistant cotton plants have high insect resistance in the early stage of development, but relatively low resistance in the late stage. Substituting a reproductive organ-specific promoter for the CaMV35S promoter presently being used could be an ideal solu-tion. For the first time, the promoter sequence of ADP-ribosylation factor 1 (arf1) gene was iso-lated from Gossypium hirsutumY18 by means of inverse PCR. The sequencing result discovered the unique structure of the arf1 promoter, including four promoter-specific elements, the initiator, TATA box, CAAT box and GC box, and also an intron in 5′-untranslation region. Four plant ex-pression vectors were constructed for functional analysis of the promoter. Based on the pBI121 plant expression vector, four truncated arf1 promoters took the place of the CaMV35S promoter. These vectors were different only in their promoter regions. They were introduced into cotton plants via pollen tube pathway. Histochemical GUS staining and fluorescence quantitative analyses were performed to examine the expression patterns of the GUS gene driven by the 4 arf1 truncated promoters in transgenic cotton plants respectively. The results showed that the arf1 promoter was a typical reproductive organ-specific promoter. Hopefully, the arf1 promoter can be a regulatory element for designing cotton reproductive organs with desired characteris-tics.

关键词： cotton plant, arf1 promoter, reproductive organ, preferential expression, functional identification.

“四性”原则在“园林花卉学”课程中的应用探索

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南方农业 2021年第13期15卷 79-81页

作者：董攀任茂智重庆大学生命科学学院重庆401331

在综合性大学探索开设非限制性选修课程“园林花卉学”,有利于提高学生的文化素养和科学素质。为了不断提高“园林花卉学”教学质量和效果,结合多年来在教学内容和设计上进行的一系列改革与实践,探讨了“四性”(观赏性、实用性、科学性... 详细信息

在综合性大学探索开设非限制性选修课程“园林花卉学”,有利于提高学生的文化素养和科学素质。为了不断提高“园林花卉学”教学质量和效果,结合多年来在教学内容和设计上进行的一系列改革与实践,探讨了“四性”(观赏性、实用性、科学性、趣味性)原则在“园林花卉学”课程建设中的具体应用。

关键词：园林花卉学教学改革四性非限制性选修课

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月球生命保障前沿基地探究

前瞻科技 2024年第1期3卷 137-145页

作者：谢更新程浩王莹任茂智重庆大学教育部深空探测联合研究中心重庆400044 中国农业科学院都市农业研究所国家成都农业科技中心成都610213

月球是人类进行深空探测和太空探索的前哨站。在月球建立生命保障前沿基地可为人类移居月球提供食物保障。文章介绍了近年来国内外主要的探月工程,系统总结了人类在空间站、月球、生命保障系统等发展太空农业的现状。围绕月球生命保障... 详细信息

月球是人类进行深空探测和太空探索的前哨站。在月球建立生命保障前沿基地可为人类移居月球提供食物保障。文章介绍了近年来国内外主要的探月工程,系统总结了人类在空间站、月球、生命保障系统等发展太空农业的现状。围绕月球生命保障前沿基地发展面临的挑战,文章提出了发掘及利用月球南极资源、创制适于月球生长的功能植物品种、建立基于月球熔岩洞穴的生命保障前沿基地等建议,以期为中国太空农业发展提供参考。

关键词：探月工程月球南极功能植物太空农业

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茉莉酸信号传导在植物抗逆性方面研究进展

中国农业科技导报 2015年第2期17卷 17-24页

作者：宋云李林宣卓凤萍张雪妍任茂智李付广中国农业科学院棉花研究所棉花生物学国家重点实验室河南安阳455000 重庆大学生命科学学院重庆400030

茉莉酸(jasmonic acid,JA)作为一种重要的新型植物内源激素广泛参与调控植物防御反应和形态发育相关的多种生物学过程。近年来,越来越多的研究发现茉莉酸在调控植物发育和渗透胁迫应答等方面发挥着重要作用。从茉莉酸信号传导途径中的... 详细信息

茉莉酸(jasmonic acid,JA)作为一种重要的新型植物内源激素广泛参与调控植物防御反应和形态发育相关的多种生物学过程。近年来,越来越多的研究发现茉莉酸在调控植物发育和渗透胁迫应答等方面发挥着重要作用。从茉莉酸信号传导途径中的关键节点基因对根、气孔的发育以及对光合作用的影响阐述了该信号途径在植物抗逆性方面的最新研究进展,从而为全面认识茉莉酸及其信号途径在植物抗旱、耐盐碱方面所发挥的作用提供参考。

关键词：茉莉酸信号传导抗逆

马铃薯Shaker基因家族全基因组鉴定和分析

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重庆科技学院学报（自然科学版） 2019年第3期21卷 104-108,116页

作者：尹欢卓凤萍邓可宣任茂智重庆大学生命科学学院重庆401331 重庆科技学院重庆401331

钾素营养在植物的生长发育过程中具有重要作用。Shaker家族基因参与植物对低钾环境胁迫的响应过程,可帮助植物从土壤中吸收钾素。将拟南芥Shaker蛋白质序列作为源序列,在马铃薯数据库PGSC中搜索获得22个StShaker基因;通过多序列比对和... 详细信息

钾素营养在植物的生长发育过程中具有重要作用。Shaker家族基因参与植物对低钾环境胁迫的响应过程,可帮助植物从土壤中吸收钾素。将拟南芥Shaker蛋白质序列作为源序列,在马铃薯数据库PGSC中搜索获得22个StShaker基因;通过多序列比对和保守结构域分析,最终从马铃薯基因组中鉴定出9个StShaker基因。通过分析其外显子内含子结构、系统发育树、染色体分布、蛋白质序列等,发现StShaker基因能够分为5组,有4个保守的结构域,所有的StShaker基因在结构域I中都有一个核心的GYGDE基序。低钾胁迫对大多数StShaker基因在转录水平上没有显著影响,StShaker可能是受转录后水平上的调控来影响马铃薯对钾离子的吸收。

关键词： Shaker基因家族马铃薯钾离子吸收表达模式系统发育

分离植物目的基因全长cDNA和启动子的新方法——快速定位转录起始位点(RITIS)

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分子植物育种 2006年第1期4卷 23-28页

作者：冯丽任茂智罗洪发何光华西南大学农学与生命科学学院重庆400716 上海交通大学生命科学与技术学院上海200030

启动子是调控外源基因在植物体内表达的“开关”。随着植物转基因技术的广泛应用,无论是基础研究还是应用研究,人们希望能够充分利用启动子来准确控制外源基因在植物体内的表达,使目的基因的“开”和“关”、表达的“多”和“少”、在... 详细信息

启动子是调控外源基因在植物体内表达的“开关”。随着植物转基因技术的广泛应用,无论是基础研究还是应用研究,人们希望能够充分利用启动子来准确控制外源基因在植物体内的表达,使目的基因的“开”和“关”、表达的“多”和“少”、在“何地”和“何时”表达等,能够听从人的指挥,以实现植物育种的分子设计。因此,快速分离和鉴定植物体内各种特异启动子已经成为植物基因工程研究的热点和难点。本文在互补末端连接反向PCR(CELI-PCR)技术基础上建立起一种快速分离目的基因全长cDNA和启动子序列的新方法。该方法利用CELI-PCR进行染色体连续步移,获取足够长的目的基因及其上游基因组DNA序列,再根据转录起始位点是目的基因转录本和启动子的分界点,其下游转录本中的外显子可通过RT-PCR扩增,而上游启动子序列则不能被RT-PCR扩增这一特点,借助RT-PCR进行cDNA连续步移,直到获得全长cDNA,确定启动子基因5'非翻译区的位置,进而精确定位转录起始位点。从而获取目的基因准确的启动子序列和全长cDNA序列。因此,建立在CELI-PCR基础上的RITIS技术,可绕过繁琐的构建cDNA库和5'-RACE等方法快速分离目的基因全长cDNA和启动子序列。

关键词：启动子全长cDNA 转录起始位点