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Cytogenetical Changes among Polyembryonic(PEm)and Non-PEm Maize Plants

Phyton-International Journal of Experimental Botany 2021年第1期90卷 87-97页

作者： A.A.román-Calzoncit F.ramírez-Godina J.Sánchez-Laureano A.C.Flores-Gallegos J.Espinoza-Velázquez r.rodríguez-herrera School of Chemistry Universidad Autónoma de Coahuila.Blvd.V.Carranza e Ing.JoséCárdenas V.s/n.Col.República Ote.C.P.25280.SaltilloCoahuilaMéxico Plant Breeding Department Universidad Autónoma Agraria Antonio NarroBlvd.Antonio Narro 1923BuenavistaSaltilloMéxico

Polyembryony in maize(PEm)contributes to improving the nutritional properties of the grain,as well as an increase in yield,since it generates multiple plants per seed,opening the possibility of developing new ***,it is unknown whether polyembryony in maize is the product of chromosomal *** on the above,in this research a cytogenetic study was proposed to verify if chromosomal abnormalities are related to the maize *** a meiotic study,maize genotypes with variable proportions of polyembryony(PEm),from the UA-IMM-BAP population and non-PEm(monoembryonic)maize were used,while for a mitosis analysis,30 families of maternal half-siblings(MHS)from two different populations with high polyembryony,denominated as BHP(brachytic,high polyembryony)and NHP(normal height,high polyembryony)were *** these genotypes were planted in the Buenavista Agricultural Experimental Station-UAAAN at Saltillo,Coahuila,*** frequency of PEm was estimated in all *** was found that the polyembryony occurs at frequencies from 34 to 66%in the D-02,BHP group,and from 13 to 21%in the segregating groups of the G3-0202 and *** squash technique was used for both cytogenetic *** meiotic irregularities in maize chromosomes were detected,such as irregular association of 10%of chromosomes in metaphase 1,and agglomeration of chromatin in 100%of the *** addition,pollen viability was estimated by the staining technique with 1%acetocarmine dye,and it was found that the polyembryonic ones have pollen viability of 100%.In meiosis in prophase,I sub-phase diakinesis,ten pairs of bivalents were confirmed,confirming 2n=20,both in non-PEm and PEm maize,corroborating mating,and balanced segregation,without the presence of univalent or *** the chromosomal count carried out in cells in mitosis,only in the BHP-200 polyembryonic family was registered two out of twenty triploid *** condition of ploidy is atypical in

关键词： Zea mays irregular meiosis mitosis chromosomes chromatin polyploidy

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Microwave-ultrasound assisted extraction of red corn pigments and their effect on chemical composition and tecno-functional properties

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Food Bioscience 2022年第6期50卷 1111-1119页

作者： J.D.Garcia-Ortiz A.C.Flores-Gallegos J.A.Ascacio-Valdés C.M.López-Badillo S.D.Nery-Flores S.C.Esparza-González r.rodríguez-herrera School of Chemistry Autonomous University of CoahuilaCampus SaltilloSaltilloCoahuila25280Mexico School of Dentistry Autonomous University of CoahuilaCampus SaltilloSaltilloCoahuila25125Mexico

Currently, there is a growing interest in obtaining natural pigments, without presenting a risk to health, as well as to the matrix and food safety. The objective of this study was to characterize grains with different shades of red color (High pigmentation maize = rA, Medium pigmentation maize = rM, and Low pigmentation maize = rB, reddish maize with white segments = VO and white maize control = SC). The physical properties (kernel weight, length, width, thickness, and grain and flour color), chemical composition (moisture, fat, protein, fiber, starch, ash, and minerals), and techno-functional properties (capacity of absorption of organic molecules (OMAC), swelling (CH), oil retention (CAAC), and water absorption (WAS)) variables were measured. Afterward, rM was selected, and a pigment extraction (EAUM) was carried out using microwave-ultrasound equipment with different ratios of ethanol (0, 30, and 70%) and citric acid ratios (0 and 1%). The color and properties of the solid (starch, protein, fat, and their techno-functional properties) were determined in the aqueous phase and were compared with the properties prior to EAUM. The results indicated that the red grains have higher starch content (57.6%) and reddish colors, in addition, VO presented higher moisture (10.4%), fat (6.7%) and fiber (10.0%). The colors of the pigments obtained with EtOH (30 and 70%) show shades red and yellow. Finally, the EAUM decreased the fat content up to 57%, increased WAS and CAAC in the residual flour and generated changes in OMAC and CH, increasing in aqueous medium and reducing with EtOH.

关键词： Natural colorant Zea mays Green extraction Proximal analysis NIr Agronomic properties

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Non-human primate pluripotent stem cells for the preclinical testing of regenerative therapies

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Neural regeneration research 2022年第9期17卷 1867-1874页

作者： Ignacio rodríguez-Polo rüdiger Behr research Platform Degenerative Diseases German Primate Center-Leibniz Institute for Primate ResearchGöttingenGermany German Center for Cardiovascular research(DZHK) Partner Site GöttingenGöttingenGermany

Non-human primates play a key role in the preclinical validation of pluripotent stem cell-based cell replacement *** stem cells used as advanced therapy medical products boost the possibility to regenerate tissues and organs affected by degenerative ***,the methods to derive human induced pluripotent stem cell and embryonic stem cell lines following clinical standards have quickly developed in the last 15 *** the preclinical validation of cell replacement therapies in non-human primates,it is necessary to generate non-human primate pluripotent stem cell with a homologous quality to their human ***,pluripotent stem cell technologies have developed at a slower pace in non-human primates in comparison with human cell *** recent years,however,relevant progress has also been made with non-human primate pluripotent stem *** review provides a systematic overview of the progress and remaining challenges for the generation of non-human primate induced pluripotent stem cells/embryonic stem cells for the preclinical testing and validation of cell replacement *** focus on the critical domains of(1)reprogramming and embryonic stem cell line derivation,(2)cell line maintenance and characterization and,(3)application of non-human primate pluripotent stem cells in the context of selected preclinical studies to treat cardiovascular and neurodegenerative disorders performed in non-human primates.

关键词： embryonic stem cells induced pluripotent stem cells non-human primates pluripotent stem cells preclinical regeneration reprogramming translational research

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Fungal contamination of stored automobile-fuels in a tropical environment

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Journal of Environmental Sciences 2010年第10期22卷 1595-1601页

作者： Carlos E.rodríguez-rodríguez Evelyn rodríguez rigoberto Blanco Ivannia Cordero Daniel Segura research Center in Tropical Diseases and Laboratory of research in Anaerobic Bacteriology Faculty of MicrobiologyUniversity of Costa Rica Department of Chemical Engineering School of EngineeringAutonomous University of Barcelona research Center in Electrochemistry and Chemical Energy University of Costa Rica

Because of the lack of reports, the base levels of microbial contamination on stored fuels are unknown in tropical regions and it is unclear whether these levels have some influence on fuel quality parameters. Therefore, fungal quality in automobile fuels stored across Costa rican territory was evaluated during two years according to the standard ASTM D6974-04. For a total of 96 samples, counts and identification of molds and yeasts were performed on regular gas, premium gas and diesel taken from the bottom and superior part of the container tanks. The highest contamination was found on the bottom of the tanks, where an aqueous phase was usually identified, showing populations over the ones present in the hydrocarbon itself （up to 108 CFU/L）. Diesel was the most contaminated fuel （up to 107 CFO/L）; however, an alteration on the physicochemical parameters was not observed in any kind of fuel. Seventy-five mold strains were isolated, Penicillium sp. being the most common genus （45.8% of the samples）, and ten yeast strains, from the genera Candida sp. and rhodotorula sp. Four of the yeasts were able to grow on diesel as the sole carbon source, at concentrations ranging from 0.5% to 25%. Increasing the frequency of tank cleaning, adding antimicrobial agents and monitoring microbial populations are recommended strategies to improve microbial quality of stored fuels.

关键词： fuel microbial contamination biodegradation molds yeasts

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Origin and evolution of the genera Lonsdaleia and Actinocyathus:Insights for the Mississippian palaeogeography from the western Palaeotethys

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Journal of Palaeogeography 2023年第2期12卷 296-310页

作者： Isabel rodríguez-Castro Ian D.Somerville Sergio rodríguez Departamento de Geodinamica Estratigrafía y PaleontologíaUniversidad Complutense de Madrid28040MadridSpain UCD School of Earth Sciences University College DublinBelfieldDublin 4Ireland Instituto de Geociencias(CSIC UCM)Ciudad Universitaria28040MadridSpain

representatives of the subfamily Lonsdaleiinae Chapman,1893 are common in the Mississippian of the western Palaeotethys.A general analysis of the origin,distribution and evolution of that subfamily has been *** most probable hypothesis for the origin of the genus Lonsdaleia McCoy,1849 is to acquire colonialism via the genus Axophyllum Milne Edwards and Haime,*** d'Orbigny,1849 would be a descendant of Lonsdaleia by increasing integration in the *** first occurrences of Lonsdaleia have been recorded in the lower Visean from northern Britain and northern Tianshan Mountains of northwestern China,but the diversification and migration to the whole Palaeotethys only happened in the late *** hypotheses are proposed on that *** Serpukhovian was also a period of migrations and diversification for these *** Lonsdaleia and Actinocyathus have been recorded in Bashkirian refuges,the Sverdrup Basin in northern Laurasia and the Tindouf Basin in northern Africa,*** division of the western Palaeotethys into six subprovinces based on the distribution of corals is proposed.

关键词： Carboniferous Palaeotethys Biogeography Evolution rugosa

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Drying Kinetics and Energy Consumption in Vacuum Drying Process with Microwave and radiant Heating

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Chinese Journal of Chemical Engineering 2004年第6期12卷 809-813页

作者： M.Kamel J.I.Lombrana C.de Elvira r.rodríguez Department of Chemical Engineering Facultad de Ciencias Universidad del Pais Vasco. Apdo. 644 48080 Bilbao SpainDepartment of Chemical Engineering Facultad de Ciencias Universidad del Pais Vasco. Apdo. 644 48080 Bilbao SpainInstituto del Frio (CSIC) Ciudad Universitaria. 28040 Madrid SpainDepartment of Chemical Engineering Facultad de Ciencias Universidad del Pais Vasco. Apdo. 644 48080 Bilbao Spain

The general objective of this work is to analyze energy input in a vacuum process with the incorporation of microwave heating. Thus, necessary criteria for designing an efficient freeze-drying operation are considered through the analysis of strategies based on the combination of different intensities of radiant and microwave *** other aim of this research topic is to study the kinetics of drying in relation to mass transfer *** freeze-drying strategies using both heating systems were used. Consequently, energy input could be related to diffusivity coefficients, temperature and pressure profiles during dehydration of the product and analyzed in comparison to a conventional freeze-drying process.

关键词： vacuum microwave drying diffusivity constant energy

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Complexity of adaptive testing in scenarios defined extensionally

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Frontiers of Computer Science 2023年第3期17卷 11-28页

作者： Ismael rodrÍguez David rUBIO Fernando rUBIO Dpto.Sistemas Informáticos y Computación Facultad de InformáticaUniversidad Complutense de MadridMadrid 28040Spain Instituto de Tecnologías del Conocimiento Universidad Complutense de MadridMadrid 28040Spain Instituto de Biomecánica de Valencia.Universitat Politècnica de València València 46022Spain

In this paper,we consider a testing setting where the set of possible definitions of the Implementation Under Test(IUT),as well as the behavior of each of these definitions in all possible interactions,are extensionally defined,i.e.,on an element-by-element and case-by-case *** this setting,the problem of finding the minimum testing strategy such that collected observations will necessarily let us decide whether the IUT is correct or not(i.e.,whether it necessarily belongs to the set of possible correct definitions or not)is studied in four possible problem variants:with or without non-determinism;and with or without more than one possible definition in the sets of possible correct and incorrect *** computational complexity of these variants is studied,and properties such as PSPACE-completeness and Log-APX-hardness are identified.

关键词： formal testing adaptive testing computational complexity PSPACE-completeness approximation hardness

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Observation of performance measures of STEMI in elderly after implementation of an updated protocol:results from a single center without coronary interventions

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Journal of Geriatric Cardiology 2022年第11期19卷 930-936页

作者： Miguel rodríguez-ramos General Hospital Camilo Cienfuegos Bartolome Maso and Mirto Streets Sancti SpiritusCuba

As life expectancy increases,heart conditions,and especially acute coronary syndromes(ACS),are becoming a greater health issue.[1-3]Accordingly,the number of elderly patients in registries of myocardial infarction,is increasingly growing.[4,5]However,elderly patients are less likely to be treated with invasive strategy compared to younger patients,despite their intrinsic greater risk.[6,7]Several reports have described how quality initiatives improve treatment of ST elevation myocardial infarction(STEMI).

关键词： coronary STEMI Observation

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Is the kafirin profile capable of modulating the ileal digestibility of amino acids in a soybean meal-sorghum diet fed to pigs?

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Animal Nutrition 2019年第2期5卷 124-129页

作者： Víctor A.Balderrama-Pérez JoséG.Gómez-Soto Tércia C.reis de Souza Ericka r.rodríguez Gerardo Mariscal-Landín Facultad de Estudios Superiores Cuatitlán Universidad National Autónoma de MéxicoCuautitlan54714Mexico Facultad de Cientias Naturales Universidad Autónoma de QuerétaroQuerétaro76230Mexico Centro Nacional de Investigación Disciplinaria en Fisiología y Mejoramiento Animal Instituto Nacional de Investigaciones Forestales Agrícolas y PecuariasAjuchitilan76380Mexico

The effects of kafirins on protein and amino acid ileal digestibility have not been evaluated in vivo in *** aim of this study was to determine the effects of protein profile on apparent ileal digestibility(AID)of amino acids. We used a sorghum hybrid with low tannin content(rid was harvested from 2 different plots with different kafirin profile. Sorghum with greater content of total kafirins had less content of y-and al-kafirins and higher content of β-and a2-kafirins than that with lower content of total kafirins. Two sorghum-soybean meal(SBM) diets were formulated: 1) low kafirin(LK) content(32.2 g/kg) and 2) high kafirin(HK) content(48.1 g/kg). A control diet(maize-SBM) and a reference SBM-diet were also prepared. The reference diet was fed to all pigs following the experimental period and was used to estimate the AID of cereals by the difference method. 'T' cannulas were fixed in the distal ileum of 18 barrows(6 by treatment), divided into 2 groups of 9 pigs. The pigs were fed 2.5 times their maintenance requirement of digestible energy(110 kcal/kg BW00.75). The AID of dry matter,protein, amino acids, and energy of the experimental diets was measured;the AID of cereals(maize, LK sorghum and HK sorghum) was estimated by the difference method. The maize-SBM diet was more digestible than the sorghum-SBM diets, only with respect to valine(P r than that in sorghum-SBM diets. The changes in kafirin profile between the diets only affected the AID of threonine(P reased by 9.5 percentage units in LK diet compared with HK diet. regarding the AID of cereals, maize exhibited greater AID than sorghum, with respect to valine(P rine(P rison of sorghum with LK and HK content showed that the AID of threonine and serine increased by 50.5(P rcentage units(P r, respec:tively. The higher content of γ-kafirins in L

关键词： Amino acids Ileal digestibility Kafirin Pig Sorghum

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The adaptive value of grass traits in response to grazing

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Journal of Plant Ecology 2018年第2期11卷 248-255页

作者： Valerie Cayssials Claudia rodríguez Instituto de Ecología y Ciencias Ambientales Facultad de CienciasUniversidad de la RepúblicaIguá4225CP 11400MontevideoUruguay

Aims Grazing is associated with several plant traits that may confer resist-ance to ***,cross-species analyses do not allow for the differentiation between adaptive evolution and common *** this study,we evaluated the effect of grazing on 5 mor-phological traits in 41 native grasses growing in natural grasslands of Uruguay and investigated whether such effects are independent of *** We used data of grass species from 17 paired,grazed and ungrazed plots located in different regions of natural grass-lands of *** each species,we calculated the Grazing response index(Gri)and estimated the culm length,blade length,blade width,blade length/width ratio and caryopsis *** values were calculated as the mean of the maximum and minimum values reported in a public *** assessed the relationship between the Gri and the morphologi-cal traits using cross-species correlations,and we re-examined the correlations using phylogenetically controlled comparative *** Findings Culm length and blade length were significantly correlated with the *** with higher culms and longer blades diminished their cover under *** association remained significant after statistical control of phylogenetic relatedness among *** contrast,blade width,blade length/width ratio and caryopsis length did not show any significant relationship with the *** studies in temperate grasslands recognized that several plant traits respond to grazing but were rarely evaluated in a phylogenetic *** results are consistent with the idea that grazing is a selective force with a clear effect on the evolution of grass stature,selecting smaller plants with shorter blades.

关键词： grasslands plant height leaf length PiCs Uruguay

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