Cytogenetical Changes among Polyembryonic(PEm)and Non-PEm Maize Plants
作者机构：School of ChemistryUniversidad Autónoma de Coahuila.Blvd.V.Carranza e Ing.JoséCárdenas V.s/n.Col.República Ote.C.P.25280.SaltilloCoahuilaMéxico Plant Breeding DepartmentUniversidad Autónoma Agraria Antonio NarroBlvd.Antonio Narro 1923BuenavistaSaltilloMéxico
出 版 物：《Phyton-International Journal of Experimental Botany》 (国际实验植物学杂志（英文）)
年 卷 期：2021年第90卷第1期
页 面：87-97页
核心收录：
基 金：This work was financed through the Project“Identification and sequencing of DNA regions that encode for maize polyembryony.”FON.SEC.SEP-CONACYT-BASIC SCIENCE CB-2015-256081-Z-3061.AARC appreciates the financial support from this project for her BSc studies
主 题：Zea mays irregular meiosis mitosis chromosomes chromatin polyploidy
摘 要：Polyembryony in maize(PEm)contributes to improving the nutritional properties of the grain,as well as an increase in yield,since it generates multiple plants per seed,opening the possibility of developing new ***,it is unknown whether polyembryony in maize is the product of chromosomal *** on the above,in this research a cytogenetic study was proposed to verify if chromosomal abnormalities are related to the maize *** a meiotic study,maize genotypes with variable proportions of polyembryony(PEm),from the UA-IMM-BAP population and non-PEm(monoembryonic)maize were used,while for a mitosis analysis,30 families of maternal half-siblings(MHS)from two different populations with high polyembryony,denominated as BHP(brachytic,high polyembryony)and NHP(normal height,high polyembryony)were *** these genotypes were planted in the Buenavista Agricultural Experimental Station-UAAAN at Saltillo,Coahuila,*** frequency of PEm was estimated in all *** was found that the polyembryony occurs at frequencies from 34 to 66%in the D-02,BHP group,and from 13 to 21%in the segregating groups of the G3-0202 and *** squash technique was used for both cytogenetic *** meiotic irregularities in maize chromosomes were detected,such as irregular association of 10%of chromosomes in metaphase 1,and agglomeration of chromatin in 100%of the *** addition,pollen viability was estimated by the staining technique with 1%acetocarmine dye,and it was found that the polyembryonic ones have pollen viability of 100%.In meiosis in prophase,I sub-phase diakinesis,ten pairs of bivalents were confirmed,confirming 2n=20,both in non-PEm and PEm maize,corroborating mating,and balanced segregation,without the presence of univalent or *** the chromosomal count carried out in cells in mitosis,only in the BHP-200 polyembryonic family was registered two out of twenty triploid *** condition of ploidy is atypical in
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