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Gapless Genome Assembly of ZH8015 and Preliminary Multi-Omics Analysis to Investigate ZH8015's Responses Against Brown Planthopper Infestation

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Rice science 2024年第3期31卷 317-327,I0042-I0045页

作者： LI Dian DUAN Wenjing LIU qun’en WU Weixun ZHAN Xiaodeng SUN Lianping ZHANG Yingxin cheng Shihua State Key Laboratory of Rice Biology and Breeding China National Rice Research InstituteHangzhou 310006China

Accurate genomic information is essential for advancing genetic breeding research in specific rice *** study presented a gapless genome assembly of the indica rice cultivar Zhonghui 8015(ZH8015)using Pac Bio HiFi,Hi-C,and ONT(Oxford Nanopore Technologies)ultra-long sequencing technologies,annotating 43037 gene ***,utilizing this genome along with transcriptomic and metabolomic techniques,we explored ZH8015's response to brown planthopper(BPH)*** transcriptomic sampling indicated significant changes in gene expression levels around 48 h after BPH *** analysis revealed particularly significant alterations in genes related to reactive oxygen species scavenging and cell wall *** results demonstrated marked increases in levels of several monosaccharides,which are components of the cell wall and dramatic changes in flavonoid *** association analysis identified differentially expressed genes associated with key metabolites,shedding light on ZH8015's response to BPH *** summary,this study constructed a reliable genome sequence resource for ZH8015,and the preliminary multi-omics results will guide future insect-resistant breeding research.

关键词： brown planthopper gapless genome genome assembly multi-omics Nilaparvata lugens rice

Catalytic asymmetric(3+3)cycloaddition between different 2-indolylmethanols

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Science China Chemistry 2024年第8期67卷 2629-2636页

作者： Tianzhen Li Sijia Liu Shufang Wu Qi cheng qun Chen Yinchun Jiao Yuchen Zhang Feng Shi School of Petrochemical Engineering School of Materials ScienceEngineeringChangzhou UniversityChangzhou 213164China Research Center of Chiral Functional Heterocycles School of Chemistry and Materials ScienceJiangsu Normal UniversityXuzhou 221116China School of Chemistry and Chemical Engineering Hunan University of Science and TechnologyXiangtan 411201China School of Chemistry and Chemical Engineering Henan Normal UniversityXinxiang 453007China

The catalytic asymmetric(3+3)cycloaddition between different 2-indolylmethanols has been established,in which a series of chiral indole-fused six-membered heterocycles were constructed in high yields with excellent *** work not only has established the first catalytic asymmetric cycloaddition between different 2-indolylmethanols but also provided a powerful strategy for constructing enantioenriched indole-fused six-membered ***,biological evaluation discovered some products with promising antitumor ***,theoretical calculations performed on the reaction pathway and activation mode provide an in-depth understanding of this catalytic asymmetric(3+3)cycloaddition among different 2-indolylmethanols,which will advance the understanding of the chemistry of indolylmethanols.

关键词： asymmetric organocatalysis chiral phosphoric acid cycloaddition enantioselectivity 2-indolylmethanol

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Effect of trichostatin A and paclitaxel on the proliferation and apoptosis of lung adenocarcinoma cells

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Chinese Medical Journal 2013年第1期126卷 129-134页

作者： ZHANG Song ZHANG qun-cheng JIANG Shu-juan Department of Respiratory Diseases Provincial Hospital Affiliatedto Shandong University Jinan Shandong 250021 China

Background Histone deacetylase inhibitors can regulate gene expression through modulation of the degree of acetylation of histone and non-histone, thus affecting cell proliferation, survival and chemosensitivity. Histone deacetylase inhibitors combined with paclitaxel may enhance the inhibitory effect of drugs on lung cancer cells. This study aimed to observe the effect of trichostatin A （TSA）/paclitaxel on the proliferation and apoptosis in human A549 lung adenocarcinoma cells, and to investigate its mechanism. Methods A549 cells were cultured in Dulbecco modified Eagle＇s medium （DMEM） in the presence of paclitaxel and the histone deacetylase inhibitor TSA, and the growth curve was obtained by trypan-blue exclusion assay and cell count. Apoptosis was assessed using Hoechst 33258 staining and flow cytometry analysis, and cell cycle was detected by flow cytometry analysis. The proteins poly ADP-ribose polymerase （PARP）, caspase-3, survivin, and tubulin acetylation were detected by Western blotting. Results A significant reduction of proliferation was observed in A549 lung adenocarcinoma cells treated by paclitaxel or TSA. Combined treatment with TSA/paclitaxel caused the greatest inhibition of cell proliferation. The combined treatment with TSA and paclitaxel induced more severe apoptosis, and significantly more cells were arrested in G2/M phase （P 〈0.05） then with a single drug. Using Western blotting, we demonstrated that treatment with TSNpaclitaxel led to synergistic increase in acetylated tubulin, PARP, caspase-3, and reduced the expression of survivin. Conclusion TSA and paclitaxel have a synergistic activity that can inhibit cell growth and induce apoptosis.

关键词： trichostatin A paclitaxel caspase-3 survivin

Toughening of BMI Based Graphite Laminates by Ex-situ Concept

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材料工程 2006年第5期34卷 41-45页

作者： cheng qun-feng XU Ya-hong YI Xiao-su Department of Polymer Science and Engineering Zhejiang University Hangzhou 310027 China National Key Laboratory of Advanced Composites Beijing Institute of Aeronautical Materials Beijing 100095 China

High-performance bismaleimdes (BMI) matrix composites reinforced by graphite fibers were prepared and toughened with a thermoplastic component (PAEK) by using the Ex-situ concept. Experimental matrix was designed for overall toughening of the base resin, periodically interleaving thermoplastic films into each plies (Ex-situ concept) and for varying the film compositions. The highest impact damage resistance characterized by compression after impact (CAI) was obtained for the laminates toughened on the Ex-situ concept, especially, when two-component cast films of a special ration of PAEK/BMI 60∶40 were interleaved though the thermoplastic concentration for the overall toughening, interleaving the pure thermoplastic films and the two-component films was comparable. There were two peaks found in the DSC trace of the laminates toughened implying a phase separation process occurred. The glass transition temperature of the laminates toughened was slightly reduced due to the low-temperature PAEK. Morphological study revealed a typical granular structure just in the interplay region as a result of spinodal decomposition and coarsening process. This was in agreement of the result of DSC investigation.

关键词： bismaleimdes compression after impact Ex-situ concept

Plants of Leptostrobus Heer （Czekanowskiales） from the Early Cretaceous and Late Triassic of China, with Discussion of the Genus

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Journal of Integrative Plant Biology 2006年第2期48卷 137-147页

作者： Xiu-qun Liu cheng-Sen Li Yu-Fei Wang Laboratory of Systematic and Evolutionary Botany Institute of Botany the Chinese Academy of Sciences Beijing 100093 China

Investigation of the Mesozoic seed plant Leptostrobus Heer from the Yangcaogou Formation of the Late Triassic and the Yixian Formation of the Early Cretaceous, Liaoning Province, China, provides new Insight Into Its general morphology and geographical distribution. The materials of L. cancer from the Yixian Formation described herein are later than all the past findings of this species and add to the record of L. cancer during the Early Cretaceous. Based on well-preserved specimens, the specific diagnosis Is slightly emended and the reconstructlon of L. cancer Is perfected. The materials from the Yangcaogou Formation of the Late Triassic are placed in L. spheericus, in addition, we review the history of investigation of the genus Leptostrobus since its establishment in 1876 and discuss the main characteristics of each species.

关键词： Early Cretaceous Late Triassic Leptostrobus Yangcaogou Formation Yixian Formation.

Influence of grain refinement on the corrosion behavior of metallic materials:A review

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International Journal of Minerals,Metallurgy and Materials 2021年第7期28卷 1112-1126页

作者： Pan-jun Wang Ling-wei Ma Xue-qun cheng Xiao-gang Li Institute of Advanced Materials and Technology University of Science and Technology BeijingBeijing 100083China National Materials Corrosion and Protection Scientific Data Center University of Science and Technology BeijingBeijing 100083China

Grain refinement can strengthen the mechanical properties of materials according to the classical Hall-Petch relationship but does not always result in better corrosion *** the past few decades,various techniques have been dedicated to refining grain,along with relevant studies on corrosion behavior,including general corrosion,pitting corrosion,and stress corrosion ***,the funda-mental consensus on how grain size influences corrosion behavior has not been *** paper reviews existing literature on the beneficial and detrimental effects of grain refinement on corrosion ***,the effects of microstructural changes(i.e.,grain boundary,dislo-cation,texture,residual stress,impurities,and second phase)resulting from grain refinement on corrosion behavior are *** grain re-finement not only has an impact on the corrosion performance,but also results in microstructural changes that have a non-negligible effect on corrosion behavior or even outweigh that of grain *** size is not the only factor affecting the corrosion behavior of metallic ma-terials;thus,the overall influence of microstructures on corrosion behavior should be understood.

关键词： corrosion behavior grain refinement microstructure high-temperature oxidation

Agronomical selection on loss-of-function of GIGANTEA simultaneously facilitates soybean salt tolerance and early maturity

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Journal of Integrative Plant Biology 2022年第10期64卷 1866-1882页

作者： Lidong Dong Zhihong Hou Haiyang Li Zhaobo Li Chao Fang Lingping Kong Yongli Li Hao Du Tai Li Lingshuang Wang Milan He Xiaohui Zhao qun cheng Fanjiang Kong Baohui Liu Guangdong Key Laboratory of Plant Adaptation and Molecular Design Guangzhou Key Laboratory of Crop Gene EditingInnovative Center of Molecular Genetics and EvolutionSchool of Life SciencesGuangzhou UniversityGuangzhou Higher Education Mega Center230 Waihuanxi RoadGuangzhou 510006China The Innovative Academy of Seed Design Key Laboratory of Soybean Molecular Design BreedingNortheast Institute of Geography and AgroecologyChinese Academy of SciencesHarbin 150081China College of Agriculture Heilongjiang Bayi Agricultural UniversityDaqingChina National Key Laboratory of Crop Genetics and Germplasm Enhancement National Center for Soybean ImprovementJiangsu Collaborative Innovation Center for Modern Crop ProductionNanjing Agricultural UniversityNanjing 210095China College of Agriculture Jilin Agricultural Science and Technology UniversityJilin 132101China

Salt stress and flowering time are major factors limiting geographic adaptation and yield productivity in soybean(Glycine max).Although improving crop salt tolerance and latitude adaptation are essential for efficient agricultural production,whether and how these two traits are integrated remains largely ***,we used a genome-wide association study to identify a major salt-tolerance locus controlled by E2,an ortholog of Arabidopsis thaliana GIGANTEA(GI).Loss of E2 function not only shortened flowering time and maturity,but also enhanced salt-tolerance in soybean.E2 delayed soybean flowering by enhancing the transcription of the core flowering suppressor gene E1,thereby repressing Flowering Locus T(FT)*** E2 knockout mutant e2^(CR) displayed reduced accumulation of reactive oxygen species(ROS)during the response to salt stress by releasing peroxidase,which functions in ROS scavenging to avoid *** and population genetic analyses also suggested that loss-of-function e2 alleles have been artificially selected during breeding for soybean adaptation to high-latitude regions with greater salt *** findings provide insights into the coupled selection for adaptation to both latitude and salt stress in soybean;and offer an ideal target for molecular breeding of early-maturing and salt-tolerant cultivars.

关键词： floweringtime GIGANTEA ROS saltstress soybean

Machining distortion prediction of aerospace monolithic components

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2009年第5期10卷 661-668页

作者： Yun-bo BI qun-lin cheng Hui-yue DONG Ying-lin KE State Key Lab of Fluid Power Transmission and Control Zhejiang University Hangzhou 310027 China Shanghai Spaceflight Precision Machinery Research Institute Shanghai 201600 China

To predict the distortion of aerospace monolithic components, a model is established to simulate the numerical control (NC) milling process using 3D finite element method (FEM). In this model, the cutting layer is simplified firstly. Then, the models of cutting force and cutting temperature are established to gain the cutting loads, which are applied to the mesh model of the part. Finally, a prototype of machining simulation environment is developed to simulate the milling process of a spar. Key factors influencing the distortion, such as initial residual stress, cutting loads, fixture layout, cutting sequence, and tool path are considered all together. The total distortion of the spar is predicted and an experiment is conducted to validate the numerical results. It is found that the maximum discrepancy between the simulation results and experiment values is 19.0%

关键词： Monolithic component Machining distortion Finite element simulation

Extraction of Tungsten and Molybdenum by Various Organic Compounds as Extractants

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Rare Metals 1989年第3期9卷 15-19页

作者： Yuan, qun cheng, Xin Luo, Jiazhen Yuan, chengye Shanghai Inst of Organic Chemistry China

Seventy organic compounds including various organophosphorus esters, amines and oxygen-based ligands were investigated as extractants. The experiment results show that amines are excellent extractants for W and Mo. Their sequence of extraction ability for W is as follows: quarternary amine > tertiary amine > secondary amine > primary amine. Acidic organophosphorus extractants do not extract W, but can extract Mo with high extraction ability from the acidic solution. These extractants could provide a potential process for separating W from Mo.

关键词： Alcohols Applications Amines Applications Molybdenum Ore Treatment Solvent Extraction Phosphorus Compounds Organic Solvents Organic