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preoperative biliary drainage in patients with hilar cholangiocarcinoma undergoing major hepatectomy

World Journal of Gastroenterology 2013年第46期19卷 8731-8739页

作者： Jun-Jie Xiong quentin M Nunes Wei Huang Samir pathak Ai-Lin Wei Chun-Lu Tan Xu-Bao Liu Department of Hepato-Biliary-pancreatic Surgery West China HospitalSichuan University NIHR Liverpool pancreas Biomedical Research Unit Department of Molecular and Clinical Cancer MedicineRoyal Liverpool University HospitalUniversity of Liverpool Sichuan provincial pancreatitis Center Department of Integrated Traditional Chinese and Western MedicineWest China HospitalSichuan University

AIM:To investigate the effect of preoperative biliary drainage(pBD)in jaundiced patients with hilar cholangiocarcinoma(HCCA)undergoing major liver ***:An observational study was carried out by reviewing a prospectively maintained database of HCCA patients who underwent major liver resection for curative therapy from January 2002 to December *** were divided into two groups based on whether pBD was performed:a drained group and an undrained *** baseline characteristics,preoperative factors,perioperative and short-term postoperative outcomes were compared between the two *** factors for postoperative complications were also analyzed by logistic regression test with calculating OR and 95%***:In total,78 jaundiced patients with HCCA underwent major liver resection:32 had pBD prior to operation while 46 did not have *** two groups were comparable with respect to age,sex,body mass index and ***,there was no significant difference in the total bilirubin(TBIL)levels between the drained group and the undrained group at admission(294.2±135.7 vs 254.0±63.5,p=0.126).pBD significantly improved liver function,reducing not only the bilirubin levels but also other liver *** preoperative TBIL level was significantly lower in the drained group as compared to the undrained group(108.1±60.6 vs 265.7±69.1,p=0.000).The rate of overall postoperative complications(53.1%vs 58.7%,p=0.626),reoperation rate(6.3%vs 6.5%,p=1.000),postoperative hospital stay(16.5 vs 15.0,p=0.221)and mortality(9.4%vs 4.3%,p=0.673)were similar between the two *** addition,there was no significant difference in infectious complications(40.6%vs 23.9%,p=0.116)and noninfectious complications(31.3%vs 47.8%,p=0.143)between the two *** and multivariate analyses revealed that preoperative TBIL>170μmol/L(OR=13.690,95%CI:1.275-147.028,p=0.031),Bismuth-Corlette classification(OR=0.013,95%CI:0.001-0.166,p=0.001)and extended

关键词： Obstructive jaundice Hilar cholangiocar-cinoma preoperative biliary drainage Major hepatec-tomy Surgical outcome

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Nuclear Aurora kinase A triggers programmed death-ligand 1-mediated immune suppression by activating MYC transcription in triple-negative breast cancer

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Cancer Communications 2021年第9期41卷 851-866页

作者： Shulan Sun Wei Zhou Xiaoxi Li Fei peng Min Yan Yajing Zhan Fan An Xiaoyan Li Yunyong Liu quentin Liu Haozhe piao Central Laboratory Cancer Hospital of China Medical UniversityDalian Medical University Clinical Oncology CollegeLiaoning Cancer Hospital&InstituteShenyangLiaoning 110042P.R.China Institute of Cancer Stem Cell Dalian Medical UniversityDalianLiaoning 116044P.R.China State Key Laboratory of Oncology in South China Sun Yat-sen University Cancer CenterGuangzhouGuangdong 510060P.R.China Department of pathology Cancer Hospital of China Medical UniversityLiaoning Cancer Hospital and instituteShenyangLiaoning 110042P.R.China Department of Cancer prevention and Treatment Cancer Hospital of China Medical UniversityLiaoning Cancer Hospital&InstituteShenyangLiaoning 110042P.R.China Department of Neurosurgery Cancer Hospital of China Medical UniversityLiaoning Cancer Hospital&InstituteShenyangLiaoning 110042P.R.China

Background:Increasing studies have reported that oncogenes regulate components of the immune system,suggesting that this is a mechanism for *** kinase A(AURKA),a serine/threonine kinase,is involved in cell mitosis and is essential for tumor cell proliferation,metastasis,and drug ***,the mechanism by which AURKA is involved in immune response regulation is ***,this study aimed to investigate the role of AURKA in immune regulation in triple-negative breast cancer(TNBC).Methods:peripheral blood mononuclear cells(pBMCs)were co-cultured with TNBC *** xCELLigence Real-Time Cell Analyzer-Mp system was used to detect the killing efficiency of immune cells on TNBC *** expression of immune effector molecules was tested by quantitative real-time polymerase chain reaction(qRT-pCR)to evaluate immune ***,to validate AURKA-regulated immune response in vivo,4T1 murine breast cancer cell line with AURKA overexpression or downregulation was engrafted into BALB/c *** distribution and proportion of immune cells in tumors were further evaluated by immunohistochemistry and flow ***:Downregulation of AURKA in TNBC cells increased immune response by activating CD8^(+)T cell proliferation and *** rather than cytoplasmic AURKA-derived programmed death-ligand 1(pD-L1)expression was independent of its kinase *** investigations showed that nuclear AURKA increased pD-L1 expression via an MYC-dependent ***-L1 overexpression mostly reversed AURKA silencing-induced expression of immune effector molecules,including interleukin-(IL-2),interferon-γ(IFN-γ),and ***,AURKA expression was negatively correlated with the enrichment and activity of tumor-infiltrating CD8^(+)T cells in 4T1 engrafted BALB/c mouse ***:Nuclear AURKA elevated pD-L1 expression via an MYCdependent pathway and contributed to immune evasion in *** targeting nuclear AU

关键词： Aurora kinase A immune evasion immunotherapy MYC programmed death-ligand 1 triplenegative breast cancer

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Effectiveness of Combined Biochar and Lignite with poultry Litter on Soil Carbon Sequestration and Soil Health

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Open Journal of Soil Science 2023年第2期13卷 124-149页

作者： Ardeshir Adeli John p. Brooks Dana Miles Todd Mlsna Read quentin Johnie N. Jenkins USDA-ARS Mississippi USA Mississippi State University Mississippi USA USDA-ARS-SEA Genetics and Sustainable Agricultural Research Unit Mississippi USA

Healthy soils are important to ensure satisfactory crop growth and yield. poultry litter (pL), as an organic fertilizer, has proven to supply the soil with essential macro and micronutrients, enhance soil fertility, and improve crop productivity. Integrating this treatment has the potential to improve soil physical and biological properties by increasing soil carbon, C. However, rapid decomposition and mineralization of pL, particularly in the hot and humid southeastern U.S., resulted in losing C and reduced its effect on soil health. Biochar and lignite have been proposed to stabilize and mitigate C loss through application of fresh manure. However, their combined effects with pL on C sequestration and soil health components are limited. A field experiment was conducted on Leeper silty clay loam soil from 2017 to 2020 to evaluate the combined effect on soil properties when applying biochar and lignite with pL to cotton (Gossypium hirsutum L.). The experimental design was a randomized complete block involving nine treatments replicated three times. Treatments included pL and inorganic nitrogen, N, fertilizer with or without biochar and lignite, and an unfertilized control. Application rates were 6.7 Mgkg⋅ha−1p> for pL, 6.7 Mgkg⋅ha−1p>p> for biochar and lignite and 134 kg⋅ha−1p>p>p> for inorganic N fertilizer. Integration of pL and inorganic fertilizer with biochar and lignite, resulted in greater soil infiltration, aggregate stability, plant available water, reduced bulk density and penetration resistance as compared to the sole applications of pL and inorganic fertilizer.

关键词： Soil Health Lignite Biochar poultry Litter

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The suppression of cervical cancer ferroptosis by macrophages:The attenuation of ALOX15 in cancer cells by macrophages-derived exosomes

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Acta pharmaceutica Sinica B 2023年第6期13卷 2645-2662页

作者： Yanlin Luo Yibing Chen Huan Jin Benxin Hou Hongsheng Li Xiang Li Lingfeng Liu Yuan Zhou Yonghua Li Yong Sang Song quentin Liu Zhengzhi Zou Department of Gynecologic Oncology the Affiliated Cancer Hospital of Zhengzhou University&Henan Cancer HospitalZhengzhou 450001China MOE Key Laboratory of Laser Life Science&Institute of Laser Life Science Guangdong Provincial Key Laboratory of Laser Life ScienceCollege of BiophotonicsSouth China Normal UniversityGuangzhou 510631China Institute of Clinical pharmacology School of Basic Medical ScienceAcademy of Medical ScienceZhengzhou UniversityZhengzhou 450001China Genetic and prenatal Diagnosis Center Department of Gynecology and ObstetricsFirst Affiliated HospitalZhengzhou UniversityZhengzhou 450001China Department of General Surgery the Third People's Hospital of Hainan ProvinceSanya 572000China Department of Breast Surgery Affiliated Cancer Hospital&Institute of Guangzhou Medical UniversityGuangzhou 510095China Department of pathophysiology School of Basic Medical SciencesZhengzhou UniversityZhengzhou 450001China Department of Obstetrics and Gynecology Cancer Research InstituteCollege of MedicineSeoul National UniversitySeoul 03080South Korea State Key Laboratory of Oncology in South China Cancer CenterSun Yat-sen UniversityGuangzhou 510631China Guangzhou Key Laboratory of Spectral Analysis and Functional probes College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China

Induction of cancer cell ferroptosis has been proposed as a potential treatment in several cancer ***-associated macrophages(TAMs)play a key role in promoting tumor malignant progression and therapy ***,the roles and mechanisms of TAMs in regulating tumor ferroptosis is still unexplored and remains *** study shows ferroptosis inducers has shown therapeutic outcomes in cervical cancer in vitro and in *** have been found to suppress cervical cancer cells ***,macrophage-derived miRNA-660-5p packaged into exosomes are transported into cancer *** cancer cells,miRNA-660-5p attenuates ALOX15 expression to inhibit ***,the upregulation of miRNA-660-5p in macrophages depends on autocrine IL4/IL13-activated STAT6 ***,in clinical cervical cancer cases,ALOX15 is negatively associated with macrophages infiltration,which also raises the possibility that macrophages reduce ALOX15 levels in cervical ***,both univariate and multivariate Cox analyses show ALOX15 expression is independent prognostic factor and positively associated with good prognosis in cervical ***,this study reveals the potential utility of targeting TAMs in ferroptosis-based treatment and ALOX15 as prognosis indicators for cervical cancer.

关键词： Cervical cancer Tumor-associated macrophage ALOX15 Ferroptosis Exosome miRNA-660-5p Macrophages infiltration STAT6

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prophylactic intra-peritoneal drain placement following pancreaticoduodenectomy:A systematic review and metaanalysis

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World Journal of Gastroenterology 2015年第8期21卷 2510-2521页

作者： Yi-Chao Wang p.ter Szatmary Jing-Qiang Zhu Jun-Jie Xiong Wei Huang Ilias Gomatos quentin M Nunes Robert Sutton Xu-Bao Liu Department of Thyroid and Breast Surgery West China Hospital Sichuan University NIHR Liverpool pancreas Biomedical Research Unit Royal Liverpool University Hospital University of Liverpool Department of pancreatic Surgery West China Hospital Sichuan University

AIM:To conduct a meta-analysis comparing outcomes after pancreaticoduodenectomy(pD)with or without prophylactic ***:Relevant comparative randomized and nonrandomized studies were systemically searched based on specific inclusion and exclusion *** outcomes were compared between patients with and those without routine *** odds ratios(OR)with 95%CI were calculated using either fixed effects or random effects ***:One randomized controlled trial and four non-randomized comparative studies recruiting 1728patients were *** without prophylactic drainage after pD had significantly higher mortality(OR=2.32,95%CI:1.11-4.85;p=0.02),despite the fact that they were associated with fewer overall complications(OR=0.62,95%CI:0.48-0.82;p=0.00),major complications(OR=0.75,95%CI:0.60-0.93;p=0.01)and readmissions(OR=0.77,95%CI:0.60-0.98;p=0.04).There were no significant differences in the rates of pancreatic fistula,intraabdominal abscesses,postpancreatectomy hemorrhage,biliary fistula,delayed gastric emptying,reoperation or radiologic-guided drains between the two ***:Indiscriminate abandonment of intraabdominal drainage following pD is associated with greater mortality,but lower complication *** randomized trials should compare routine vs selective drainage.

关键词： pancreaticoduodenectomy Drain Metaanalysis Morbidi

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Synergistic rare-earth yttrium single atoms and copper phosphide nanoparticles for high-selectivity ammonia electrosynthesis

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Rare Metals 2024年第11期43卷 5792-5801页

作者： Yi-Meng Cai Yang-Hua Li Yi Xiao quentin Meyer Qian Sun Wan-Jing Lai Shu-Wen Zhao Jun Li Lin-Jie Zhang Han Wang Zhang Lin Jun Luo Li-Li Han School of Materials Science and Engineering Institute for New Energy Materials and Low-Carbon TechnologiesTianjin University of TechnologyTianjin 300384China State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou 350002China School of Chemistry The University of New South WalesSydneyNSW 2052Australia School of Metallurgy and Environment Central South UniversityChangsha 410083China Chinese National Engineering Research Center for Control&Treatment of Heavy Metal pollution Changsha 410083China ShenSi Lab Shenzhen Institute for Advanced StudyUniversity of Electronic Science and Technology of ChinaShenzhen 518110China

Electrochemical nitrate reduction to NH_(3)holds a great promise for N-upcycling in nature,while its sluggish reaction kinetics involved in both the stepwise deoxygenation and hydrogenation processes necessitates the development of bespoke catalysts with multi-site ***,we report a hybrid catalyst composed of rare-earth(RE)yttrium(Y)single atoms and copper phosphide(Cu_(3)p)nanoparticles loaded on N,p-doped carbon(Y_(SA)-Cu_(3)p/CNp)through a chelating and pyrolysis *** to a synergistic contribution of Y single atoms and Cu_(3)p nanoparticles,Y_(SA)-Cu_(3)p/CNp achieves an impressive NH_(3)Faradaic efficiency(FE)of 92%at-0.5V(***)and the highest NH_(3)yield rate of11.4 mg·h^(-1)·cm^(-2)at-0.6 V(***)in an alkaline media,which surpass most of the reported *** intricate reaction pathway has been explored by online differential electrochemical mass spectrometry(DEMS),and the synergistic effect between Y single atoms and Cu_(3)p nanoparticles has been studied by in situ synchrotron X-ray absorption ***,density-functional theory(DFT)calculations unveil that the high-efficiency nitrate reduction on Y_(SA)-Cu_(3)p/CNp is attributed to a reduced energy barrier of the rate-determining deoxygenation step coupled with the enhanced stabilization of active hydrogen favorable for the hydrogenation steps,thereby boosting the overall reaction *** addition,a prototype Zn-nitrate battery utilizing Y_(SA)-Cu_(3)p/CNp as the cathode is *** work not only elucidates the mechanism behind the enhanced catalytic performance but also paves the way for the future development of highefficiency electrocatalysts through dual-site engineering.

关键词： Rare-earth single atom Cu_(3)p nanoparticle Synergistic electrocatalysis Nitrate reduction reaction Zn-nitrate battery

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The effect of the capitation policy withdrawal on maternal health service provision in Ashanti Region,Ghana:an interrupted time series analysis

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Global Health Research and policy 2022年第1期7卷 131-138页

作者： John Kanyiri Yambah Kofi Akohene Mensah Naasegnibe Kuunibe Kindness Laar Roger Ayimbillah Atinga Millicent Ofori Boateng Daniel Opoku Wilm quentin University Health Services University of EducationWinnebaGhana Department of Health policy Management and EconomicsSchool of Public HealthKwame Nkrumah University of Science and TechnologyKumasiGhana Department of Economics Faculty of Social Science and ArtsSD-Dombo University of Business and Integrated Development StudiesWaGhana Department of public Administration and Health Services Management University of Ghana Business SchoolAccraGhana Department of Commu-nity Health Ensign Global CollegeKpongGhana Department of Health Care Management Technische Universitat BerlinBerlinGermany

Background:payment methods are known to influence maternal care delivery in health *** suspended a piloted capitation provider payment system after nearly five years of *** study aimed to examine the effects of Ghana’s capitation policy on maternal health care provision as part of lesson learning and bridging this critical literature ***:We used secondary data in the District Health Information Management System-2 and an interrupted time series design to assess changes in level and trend in the provision of ANC4_(+)(visits of pregnant women making at least the fourth antenatal care attendance per month),HB36(number of hemoglobin tests conducted for pregnant women who are at the 36th week of gestation)and vaginal delivery in capitated facilities-CHpS(Community-based Health planning and Services)facilities and ***:The results show that the capitation policy withdrawal was associated with a statistically significant trend increase in the provision of ANC4+in hospitals(coefficient 70.99 ppS ***,the policy withdrawal resulted in contrasting effects in hospitals and CHpS in the trend of provision of Hb36;a statistically significant decline was observed in CHpS(coefficient−7.01,ppitals showed a statistically significant trend increase(coefficient 32.87,ppolicy withdrawal did not affect trends of vaginal delivery rates in both CHpS and ***:The capitation policy in Ghana appeared to have had a differential effect on the provision of maternal services in both CHpS and hospitals;repressing maternal care provision in hospitals and promoting adherence to anemia testing at term for pregnant women in CHpS *** makers and stakeholders should consider the possible detrimental effects on maternal care provision and quality in the design and implementation of per capita primary care systems as they can potentially impact the ac

关键词： Capitation policy Maternal care Anemia tests Delivery Hospitals CHpS

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CRISpR/Cas9 screening identifies a kinetochore-microtubule dependent mechanism for Aurora-A inhibitor resistance in breast cancer

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Cancer Communications 2021年第2期41卷 121-139页

作者： Ailin Chen Shijun Wen Fang Liu Zijian Zhang Meiling Liu Yuanzhong Wu Bin He Min Yan Tiebang Kang Eric W-F Lam Zifeng Wang quentin Liu Sun Yat-sen University Cancer Center State Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhouGuangdong 510060P.R.China Institute of Cancer Stem Cell Dalian Medical UniversityDalianLiaoning 116044P.R.China Department of Surgery and Cancer Imperial College LondonW120NNLondonUK

Background:Overexpression of Aurora-A(AURKA)is a feature of breast cancer and associates with adverse *** selective Aurora-A inhibitor alisertib(MLN8237)has recently demonstrated promising antitumor responses as a single agent in various cancer types but its phase III clinical trial was reported as a failure since MLN8237 did not show an apparent effect in prolonging the survival of ***,identification of potential targets that could enhance the activity of MLN8237 would provide a rationale for drug combination to achieve better therapeutic ***:Here,we conducted a systematic synthetic lethality CRISpR/Cas9 screening of 507 kinases using MLN8237 in breast cancer cells and identified a number of targetable kinases that displayed synthetic lethality interactions with ***,we performed competitive growth assays,colony formation assays,cell viability assays,apoptosis assays,and xenograft murine model to evaluate the synergistic therapeutic effects of Haspin(GSG2)depletion or inhibition with *** mechanistic studies,immunofluorescence was used to detect the state of microtubules and the localization of Aurora-B and mitotic centromere-associated kinesin(MCAK).Results:Among the hits,we observed that Haspin depletion or inhibition marginally inhibited breast cancer cell growth but could substantially enhance the killing effects of *** studies showed that co-treatment with Aurora-A and Haspin inhibitors abolished the recruitment of Aurora-B and mitotic centromere-associated kinesin(MCAK)to centromeres which were associated with excessive microtubule depolymerization,kinetochore-microtubule(KT-MT)attachment failure,and severe mitotic *** further showed that the combination of MLN8237 and the Haspin inhibitor CHR-6494 synergistically reduced breast cancer cell viability and significantly inhibited both in vitro and in vivo tumor ***:These findings establish Haspin as a synthetic lethal targe

关键词： alisertib Aurora-A breast cancer CHR-6494 CRISpR/Cas9 screening haspin kinetochoremicrotubule mitotic centromere-associated kinesin synthetic lethal xenograft

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逆高斯—泊松模型的真正作用及其可操作性

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情报科学 1994年第6期15卷 59-67页

作者： quentin L.Burrell Michael R.Fenton 周平

著名学者西奇尔的广义逆高斯—泊松过程分布是在情报计量模型中应用较为广泛的,具有理论研究的吸引力,由于许多情报计量数据集对于零范畴认识不足,影响了建模,文章针对其不足,推导出极大似然估计程序,克服了建模计算上的困难,使逆高斯... 详细信息

著名学者西奇尔的广义逆高斯—泊松过程分布是在情报计量模型中应用较为广泛的,具有理论研究的吸引力,由于许多情报计量数据集对于零范畴认识不足,影响了建模,文章针对其不足,推导出极大似然估计程序,克服了建模计算上的困难,使逆高斯—泊松过程分布和情报计量数据集达到较好的拟合。

关键词：情报计量模型情报学逆G-p模型

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Roux-en-Y versus BillrothⅠreconstruction after distal gastrectomy for gastric cancer:A meta-analysis

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World Journal of Gastroenterology 2013年第7期19卷 1124-1134页

作者： Jun-Jie Xiong Kiran Altaf Muhammad A Javed quentin M Nunes Wei Huang Gang Mai Chun-Lu Tan Rajarshi Mukherjee Robert Sutton Wei-Ming Hu Xu-Bao Liu Department of Hepato-Biliary-pancreatic Surgery West China HospitalSichuan University NIHR Liverpool pancreas Biomedical Research Unit Royal Liverpool University HospitalUniversity of LiverpoolLiverpool L69 3GAMerseyside CountyUnited Kingdom

AIM: To conduct a meta-analysis to compare Roux-en-Y (R-Y) gastrojejunostomy with gastroduodenal Billroth?I?(B-I) anastomosis after distal gastrectomy (DG) for gastric cancer.METHODS: A literature search was performed to identify studies comparing R-Y with B-I?after DG for gastric cancer from January 1990 to November 2012 in Medline, Embase, Science Citation Index Expanded and the Cochrane Central Register of Controlled Trials in The Cochrane Library. pooled odds ratios (OR) or weighted mean differences (WMD) with 95%CI were calculated using either ?xed or random effects model. Operative outcomes such as operation time, intraoperative blood loss and postoperative outcomes such as anastomotic leakage and stricture, bile re?ux, remnant gastritis, re?ux esophagitis, dumping symptoms, delayed gastric emptying and hospital stay were the main outcomes assessed. Meta-analyses were performed using RevMan 5.0 software (Cochrane library).RESULTS: Four randomized controlled trials (RCTs) and 9 non-randomized observational clinical studies (OCS) involving 478 and 1402 patients respectively were included. Meta-analysis of RCTs revealed that R-Y reconstruction was associated with a reduced bile re?ux (OR 0.04, 95%CI: 0.01, 0.14; p p = 0.0001), however needing a longer operation time (WMD 40.02, 95%CI: 13.93, 66.11; p = 0.003). Meta-analysis of OCS also revealed R-Y reconstruction had a lower incidence of bile re?ux (OR 0.21, 95%CI: 0.08, 0.54; p = 0.001), remnant gastritis (OR 0.18, 95%CI: 0.11, 0.29; p phagitis (OR 0.48, 95%CI: 0.26, 0.89; p = 0.02). However, this reconstruction method was found to be associated with a longer operation time (WMD 31.30, 95%CI: 12.99, 49.60; p = 0.0008).CONCLUSION: This systematic review point towards some clinical advantages that are rendered by R-Y compared to B-I?reconstruction post DG. However there is a need for further adequately powered, well-desig

关键词： Gastric cancer Distal gastrectomy Roux-en-Y Billroth I Reconstruction Meta-analysis

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