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BIOCELL 2022年第9期46卷 2133-2144页

作者： ZHENKUI CUI GUIQIN SUN quanzhi zhao College of Agronomy Henan Agricultural UniversityZhengzhou450002China Jiangxi Agricultural Engineering Vocational College Zhangshu331200China

Cotton is widely distributed worldwide,and improving the quality of its fiber is one of the most important tasks in cotton *** fibers are primarily composed of cellulose,which is synthesized by CesA complexes(CSCs).However,the functions of CesA genes in cotton fiber development have not been comprehensively *** this study,the cotton transcriptome and metabolome were used to investigate the function of CesA genes in fiber ***,321 metabolites were obtained,84 of which were associated with the corresponding ***,a target gene named Gh_A08G144300,one of the CesA gene family members,was closely correlated with the development of cotton *** target CesA gene Gh_A08G144300 was analysed to determine its specific function in cotton fiber ***-level gene expression of Gh_A08G144300 was found at different fiber development stages by RNA-seq analysis,and the silencing of Gh_A08G144300 visibly inhibited the growth of cotton fibers,showing that it is critical for their *** study provides an important reference for research on the gene function of Gh_A08G144300 and the regulatory mechanism of fiber development in cotton.

关键词： Transcriptome Metabolome CesA Cotton fiber VIGS

Co-overexpression of genes for nitrogen transport, assimilation, and utilization boosts rice grain yield and nitrogen use efficiency

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The Crop Journal 2023年第3期11卷 785-799页

作者： Jie Luo Junnan Hang Bilong Wu Xilin Wei quanzhi zhao Zhongming Fang Institute of Rice Industry Technology Research Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education)College of Agricultural SciencesGuizhou UniversityGuiyang 550025GuizhouChina Center of Applied Biotechnology Wuhan University of BioengineeringWuhan 430415HubeiChina

Nitrogen(N)fertilization is necessary for obtaining high rice *** excessive N fertilizer reduces rice plant N efficiency and causes negative effects such as environmental *** this study,we assembled key genes involved in different nodes of N pathways to boost nitrate and ammonium uptake and assimilation,and to strengthen amino acid utilization to increase grain yield and nitrogen use efficiency(NUE)in *** combinations OsNPF8.9a×OsNR2,OsAMT1;2×OsGS1;2×OsAS1,and OsGS2×OsAS2×OsANT3 optimized nitrate assimilation,ammonium conversion,and N reutilization,*** co-overexpressing rice lines obtained by co-transformation,the tiller number,biomass,and grain yield per plant of the OsAMT1;2×OsGS1;2×OsAS1-overexpressing line exceeded those of wild-type ZH11,the OsNPF8.9a×OsNR2×OsGS1;2×OsAS1-overexpressing line,and the OsGS2×OsAS2×OsANT3-overexpressing *** glutamine synthase activity,free amino acids,and nitrogen utilization efficiency(NUt E)of the OsAMT1;2×OsGS1;2×OsAS1-overexpressing line exceeded those of ZH11 and other lines that combined key genes.N influx efficiency was increased in the OsAMT1;2×OsGS1;2×OsAS1-overexpressing line and OsNPF8.9a×OsNR2×OsGS1;2×OsAS1-overexpressing line under a low ammonium and a low nitrate treatment,*** propose that combining overexpression of OsAMT1;2,OsGS1;2,and OsAS1 is a promising breeding strategy for systematically increasing rice grain yield and NUE by focusing on key nodes in the N pathway.

关键词： Nitrogen Rice tillering Grain yield Nitrogen use efficiency Co-transformation

Comparison of CWSI and T_(s)-T_(a)-VIs in moisture monitoring of dryland crops(sorghum and maize)based on UAV remote sensing

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Journal of Integrative Agriculture 2024年第7期23卷 2458-2475页

作者： Hui Chen Hongxing Chen Song Zhang Shengxi Chen Fulang Cen quanzhi zhao Xiaoyun Huang Tengbing He Zhenran Gao College of Agriculture Guizhou UniversityGuiyang 550025China Institute of New Rural Development Guizhou UniversityGuiyang 550025China Institute of Rice Industry Technology Research College of Agricultural SciencesGuizhou UniversityGuiyang 550025China

Monitoring agricultural drought using remote sensing data is crucial for precision irrigation in modern *** unmanned aerial vehicle(UAV)remote sensing,we explored the applicability of an empirical crop water stress index(CWSI)based on canopy temperature and three-dimensional drought indices(TDDI)constructed from surface temperature(T_(s)),air temperature(T_(a))and five vegetation indices(VIs)for monitoring the moisture status of dryland *** machine learning algorithms(random forest regression(RFR),support vector regression,and partial least squares regression)were used to compare the performance of the drought indices for vegetation moisture content(VMC)estimation in sorghum and *** main results of the study were as follows:(1)Comparative analysis of the drought indices revealed that T_(s)-T_(a)-normalized difference vegetation index(TDDIn)and T_(s)-T_(a)-enhanced vegetation index(TDDIe)were more strongly correlated with VMC compared with the other *** indices exhibited varying sensitivities to VMC under different irrigation regimes;the strongest correlation observed was for the TDDIe index with maize under the fully irrigated treatment(r=-0.93).(2)Regarding spatial and temporal characteristics,the TDDIn,TDDIe and CWSI indices showed minimal differences Over the experimental period,with coefficients of variation were 0.25,0.18 and 0.24,*** three indices were capable of effectively characterizing the moisture distribution in dryland maize and sorghum crops,but the TDDI indices more accurately monitored the spatial distribution of crop moisture after a rainfall or irrigation event.(3)For prediction of the moisture content of single crops,RFR models based on TDDIn and TDDIe estimated VMC most accurately(R^(2)>0.7),and the TDDIn-based model predicted VMC with the highest accuracy when considering multiple-crop samples,with R^(2)and RMSE of 0.62 and 14.26%,***,TDDI proved more effective than the CWSI in e

关键词： maize sorghum T_(s)-T_(a)-VIs CWSI UAV machine learning crop moisture monitoring

The miR167-OsARF12 module regulates rice grain filling and grain size downstream of miR159

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Plant Communications 2023年第5期4卷 246-262页

作者： Yafan zhao Xiaofan Zhang Yuan Cheng Xiangxiang Du Sachin Teotia Chunbo Miao Huwei Sun Guoqiang Fan Guiliang Tang Hongwei Xue quanzhi zhao Ting Peng Collaborative Innovation Center of Henan Grain Crops Henan Agricultural UniversityZhengzhou 450046China Key Laboratory of Rice Biology in Henan Province Henan Agricultural UniversityZhengzhou 450046China Henan Engineering Laboratory of Rice Henan Agricultural UniversityZhengzhou 450002China Joint Center for Single Cell Biology/School of Agriculture and Biology Shanghai Jiao Tong UniversityShanghai 200240China Department of Biotechnology Sharda UniversityGreater Noida 201306India Institute of Paulownia Henan Agricultural UniversityZhengzhou 450046China Department of Biological Sciences Michigan Technological UniversityHoughtonMI 49931USA College of Agriculture Guizhou UniversityGuiyang 550025China

Grain weight and quality are always determined by grain *** microRNAs have drawn attention as key targets for regulation of grain size and ***,the mechanisms that underlie grain size regulation remain largely unclear because of the complex networks that control this *** earlier studies demonstrated that suppressed expression of miR167(STTM/MIM167)substantially increased grain *** a field test,the yield increased up to 12.90%-21.94% because of a significantly enhanced grain filling ***,biochemical and genetic analyses revealed the regulatory effects of miR159 on miR167 *** analysis indicated that OsARF12 is the major mediator by which miR167 regulates rice grain *** of OsARF12 produced grain weight and grain filling phenotypes resembling those of STTM/MIM167 *** in-depth analysis,we found that OsARF12 activates OsCDKF;2 expression by directly binding to the TGTCGG motif in its promoter *** cytometry analysis of young panicles from OsARF12-overexpressing plants and examination of cell number in cdkf;2 mutants verified that OsARF12 positively regulates grain filling and grain size by targeting OsCDKF;***,RNA sequencing results suggested that the miR167-OsARF12 module is involved in the cell development process and hormone ***12-overexpressing plants and cdkf;2 mutants exhibited enhanced and reduced sensitivity to exogenous auxin and brassinosteroid(BR)treatment,confirming that targeting of OsCDKF;2 by OsARF12 mediates auxin and BR *** results reveal that the miR167-OsARF12 module works downstream of miR159 to regulate rice grain filling and grain size via OsCDKF;2 by controlling cell division and mediating auxin and BR signals.

关键词： rice miR167 OsARF12 OsCDKF 2 grainﬁlling cell cycle

Strigolactone and gibberellin signaling coordinately regulate metabolic adaptations to changes in nitrogen availability in rice

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Molecular Plant 2023年第3期16卷 588-598页

作者： Huwei Sun Xiaoli Guo Xiuli Zhu Pengyuan Gu Wei Zhang Wenqing Tao Daojian Wang Yunzhe Wu quanzhi zhao Guohua Xu Xiangdong Fu Yali Zhang National Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization Key Laboratory of Plant Nutrition and Ferilization in Low-Middle Reaches of the Yangtze RiverMinistry of AgricultureNanjing Agricultural UniversityNanjing 210095China Key Laboratory of Rice Biology in Henan Province Collaborative Innovation Center of Henan Grain CropsHenan Agricultural UniversityZhengzhou 450002China state Key Laboratoryof Plant Celland ChromosomeEngineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBejing 100049China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China

Modern semi-dwarf rice varieties of the“Green Revolution”require a high supply of nitrogen(N)fertilizer to produce high yields.A better understanding of the interplay between N metabolism and plant developmental processes is required for improved N-use efficiency and agricultural ***,we show that strigolactones(SLs)modulate root metabolic and developmental adaptations to low N availability for ensuring efficient uptake and translocation of available *** key repressor DWARF 53(D53)of the SL signaling pathway interacts with the transcription factor GROWTH-REGULATING FACTOR 4(GRF4)and prevents GRF4 from binding to its target gene promoters.N limitation induces the accumulation of SLs,which in turn promotes SL-mediated degradation of D53,leading to the release of GRF4 and thus promoting the expression of genes associated with N metabolism.N limitation also induces degradation of the DELLA protein SLENDER RICE 1(SLR1)in an D14-and D53-dependent manner,effectively releasing GRF4 from competitive inhibition caused by ***,our findings reveal a previously unrecognized mechanism underlying SL and gibberellin crosstalk in response to N availability,advancing our understanding of plant growth–metabolic coordination and facilitating the design of the strategies for improving N-use efficiency in high-yield crops.

关键词： GRF4 nitrogen-use efficiency Oryza sativa SLR1 strigolactones

Two splicing variants of amino acid transporter-like 4 (OsATL4) negatively regulate rice tillering and yield by mediating the transport of amino acids

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Crop Journal 2024年第6期12卷 1583-1596页

作者： Wang, Chuanbo Huang, Weiting Miao, Rui Wu, Bowen Wu, Wenhao He, Chongchong Zheng, Chang zhao, quanzhi Fang, Zhongming Institute of Rice Industry Technology Research Key Laboratory of Functional Agriculture of Guizhou Provincial Department of Education Key Laboratory of Molecular Breeding for Grain and Oil Crops in Guizhou Province College of Agricultural Sciences Guizhou University Guizhou Guiyang 550025 China Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education) Guizhou University Guizhou Guiyang 550025 China

Amino acids are the primary form of nitrogen utilization in higher plants, mainly transported by amino acid transporters. In this study, we analyzed the natural variation of amino acid transporter-like 4 (OsATL4) in rice germplasm resources, identified its spatiotemporal expression characteristics, determined its substrate transport, and validated its function using transgenic plants. We found that the promoter sequence of OsATL4 varied across 498 rice varieties. The expression level of OsATL4 was higher in japonica rice, which was negatively correlated with tiller number and grain yield. OsATL4 was highly expressed in the basal part, leaf sheath, stem, and young panicle, with its two splicing variants localized to the cell membrane. OsATL4a (the long splicing variant) had a high affinity for transporting Ser, Leu, Phe, and Thr, while OsATL4b (the short splicing variant) had a high affinity for transporting Ser, Leu, and Phe. Blocking OsATL4 promoted axillary bud outgrowth, rice tillering, and grain yield, whereas overexpression lines exhibited the opposite phenotype. Exogenous application of low concentrations of Ser promoted axillary bud outgrowth in overexpression lines, while high concentrations of Ser inhibited it. Conversely, the mutant lines showed the opposite response. Altered expression of OsATL4 might affect the expression of genes in nitrogen, auxin, and cytokinin pathways. We propose that two splicing variants of OsATL4 negatively regulate rice tillering and yield by mediating the transport of amino acids, making it a significant target for high-yield rice breeding. © 2024 Crop Science Society of China and Institute of Crop Science, CAAS

关键词： Amino acid Rice Splicing variants Tillering Transporter Yield

Natural variation in MORE GRAINS 1 regulates grain number and grain weight in rice

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Journal of Integrative Plant Biology 2024年第7期66卷 1440-1458页

作者： Yingchun Han Qianfeng Hu Nuo Gong Huimin Yan Najeeb Ullah Khan Yanxiu Du Hongzheng Sun quanzhi zhao Wanxi Peng Zichao Li Zhanying Zhang Junzhou Li Henan Key Laboratory of Rice Molecular Breeding and High Efﬁciency Production College of AgronomyHenan Agricultural UniversityZhengzhou 450046China Beijing Key Laboratory of Crop Genetic Improvement College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China Rice Industrial Technology Research Institute Guizhou UniversityGuiyang 550025China School of Forestry Henan Agricultural UniversityZhengzhou 450046China

Grain yield is determined mainly by grain number and grain *** this study,we identified and characterized MORE GRAINS1(MOG1),a gene associated with grain number and grain weight in rice(Oryza sativa L.),through map-based *** of MOG1 increased grain yield by 18.6%-22.3%under field *** determined that MOG1,a bHLH transcription factor,interacts with OsbHLH107 and directly activates the expression of LONELY GUY(LOG),which encodes a cytokinin-activating enzyme and the cell expansion gene EXPANSIN-LIKE1(EXPLA1),positively regulating grain number per panicle and grain *** variations in the promoter and coding regions of MOG1 between Hap-LNW and Hap-HNW alleles resulted in changes in MOG1 expression level and transcriptional activation,leading to functional *** analysis revealed that Hap-HNW,which results in a greater number and heavier grains,has undergone strong selection but has been poorly utilized in modern lowland rice *** summary,the MOG1-OsbHLH107 complex activates LOG and EXPLA1 expression to promote cell expansion and division of young panicles through the cytokinin pathway,thereby increasing grain number and grain *** findings suggest that Hap-HNW could be used in strategies to breed high-yielding temperate japonica lowland rice.

关键词： grain number grain weight MOG1 natural variation rice

A Resource for Inactivation of MicroRNAs Using Short Tandem Target Mimic Technology in Model and Crop Plants

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Molecular Plant 2018年第11期11卷 1400-1417页

作者： Ting Peng Mengmeng Qiao Haiping Liu Sachin Teotia Zhanhui Zhang Yafan zhao Bobo Wang Dongjie zhao Lina Shi Cui Zhang Brandon Le Kestrel Rogers Chathura Gunasekara Haitang Duan Yiyou Gu Lei Tian Jinfu Nie Jian Qi Fanrong Meng Lan Huang Qinghui Chen Zhenlin Wang Jinshan Tang Xiaoqing Tang Ting Lan Xuemei Chen Hairong Wei quanzhi zhao Guiliang Tang Collaborative Innovation Center of Henan Grain Crops Henan Agricultural UniversityZhengzhou 450002China College of Information and Electrical Engineering China Agricultural UniversityBeijing 100083China Department of Kinesiology and Integrative Physiology Life Science and Technology institutedMichigan Technological UniversityHoughtonMI 49931USA School of Technology Michigan Technological UniversityHoughtonMI 49931USA Guangdong Provincial Key Laboratory for Plant Epigenetics College of Life Sciences and OceanographyShenzhen UniversityShenzhen 518060P.R.China Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing Forestry UniversityBeijing 100083P.R.China Key Laboratory of Plant Stress Biology State Key Laboratory of Cotton BiologySchool of Life SciencesHenan UniversityKaifeng 475004China Department of Biotechnology Sharda UniversityGreater Noida201306India Key Laboratory of Rice Biology in Henan Province Henan Agricultural UniversityZhengzhou 450002China Department of Biological Sciences Ufe Science and Technology InstituteMichigan Technological UniversityHoughtonMI 49931USA Department of Botany and Plant Sciences Institute of Integrative Genome BiologyUniversity of CaliforniaRiversideCA 92521USA School of Forest Resources and Environmental Science Life Science and Technology InstituteMichigan Technological UniversityHoughtonMI 49931USA Department of Computer Science Michigan Technological UniversityHoughtonMI 49931USA Anhui Province Key Laboratory of Medical Physics and Technology Center of Medical Physics and TechnologyHefei Institutes of Physical ScienceChinese Academy of SciencesHefeiAnhuiChina Hefei Cancer Hospital Chinese Academy of SciencesHefeiAnhuiChina Guangzhou Institute of Biomedicine and Health Chinese Academy of SciencesGuangzhouChina

microRNAs (miRNAs)are endogenous small non-coding RNAs that bind to mRNAs and target them for cleavage and/or translational repression,leading to gene *** previously developed short tandem target mimic (STTM)technology to deactivate endogenous miRNAs in ***,we created hundreds of STTMs that target both conserved and species-specific miRNAs in Arabidopsis,tomato,rice,and maize,providing a resource for the functional interrogation of *** not only revealed the functions of several miRNAs in plant development,but also demonstrated that tissue-specific inactivation of a few miRNAs in rice leads to an increase in grain size without adversely affecting overall plant growth and ***-seq and small RNAseq analyses of STTM156/157 and STTM165/166 transgenic plants revealed the roles of these miRNAs in plant hormone biosynthesis and activation,secondary metabolism,and ion-channel activity-associated electrophysiology,demonstrating that STTM technology is an effective approach for studying miRNA *** facilitate the study and application of STTM transgenic plants and to provide a useful platform for storing and sharing of information about miRNA-regulated gene networks,we have established an online Genome Browser (https://***/***) to display the transcriptomic and miRNAomic changes in STTMinduced miRNA knockdown plants.

关键词： short tandem target mimic (STTM) miRNA RNA-seq Arabidopsis crop

HPLC法测定镉、砷胁迫下水稻根系的植物螯合肽

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环境科学学报 2011年第7期31卷 1550-1555页

作者：张静常青晓孙传范段桂兰赵全志河南农业大学水稻工程技术研究中心河南省粮食作物生理生态与遗传改良重点实验室郑州450002 中国科学院生态环境研究中心土壤环境室北京100085 中国农村技术开发中心北京100045

利用monobromobimane衍生试剂,通过反相高效液相色谱-荧光检测体系对镉、砷胁迫下水稻根系内的植物螯合肽(PCs)等巯基化合物进行形态和含量的分析测定.结果表明,采用乙腈和0.1%三氟乙酸组成的两元梯度流动相,可以将标准样品中的谷胱甘肽... 详细信息

利用monobromobimane衍生试剂,通过反相高效液相色谱-荧光检测体系对镉、砷胁迫下水稻根系内的植物螯合肽(PCs)等巯基化合物进行形态和含量的分析测定.结果表明,采用乙腈和0.1%三氟乙酸组成的两元梯度流动相,可以将标准样品中的谷胱甘肽(GSH)和PCs等巯基化合物很好地分离.利用上述方法,能较好地分离Cd、As处理下溶液培养的水稻幼苗根系内的PCs形态.Cd处理下,根系内合成PC2、PC3、PC4,其中以PC3的含量最大.As处理下,PCs的形态除PC2和PC3外,其它PCn的形态有待进一步定性.

关键词：植物螯合肽非蛋白巯基反相高效液相色谱柱前衍生水稻

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缺磷调控水稻根毛生长发育的机制

河南农业大学学报 2018年第4期52卷 519-525,532页

作者：冯凡孙虎威赵全志河南农业大学农学院河南郑州450002

通过水培试验,研究了正常供磷(300μmol·L^(-1))和缺磷(0μmol·L^(-1))处理下水稻根毛生长、根系分泌物中独脚金内酯含量和根系中生长素水平的变化以及施加外源独脚金内酯和生长素及其抑制剂对水稻根毛生长发育的影响。结果表明,与正... 详细信息

通过水培试验,研究了正常供磷(300μmol·L^(-1))和缺磷(0μmol·L^(-1))处理下水稻根毛生长、根系分泌物中独脚金内酯含量和根系中生长素水平的变化以及施加外源独脚金内酯和生长素及其抑制剂对水稻根毛生长发育的影响。结果表明,与正常供磷处理相比,缺磷胁迫下水稻的根毛和根系分泌物中的独脚金内酯含量显著增加,而根系中的生长素含量则显著降低。在缺磷条件下施加外源独脚金内酯合成抑制剂(Abamine)能够抑制根毛的生长;施加外源生长素(IAA)对根毛的生长发育没有影响;施加Abamine和IAA条件下水稻根毛的生长情况与单独施加Abamine条件下相似,在正常供磷条件下施加外源独脚金内脂(GR24)能够增加根毛数量,施加生长素运输抑制剂NPA根毛数量没有变化,施加GR24和NPA条件下水稻根毛的生长情况与单独施加GR24条件下相似。由此可以推断,缺磷胁迫下独脚金内酯可能作用于生长素的下游来调控水稻根毛的生长。

关键词：水稻根毛生长素独脚金内酯缺磷调控