A Resource for Inactivation of MicroRNAs Using Short Tandem Target Mimic Technology in Model and Crop Plants

作     者：Ting Peng Mengmeng Qiao Haiping Liu Sachin Teotia Zhanhui Zhang Yafan Zhao Bobo Wang Dongjie Zhao Lina Shi Cui Zhang Brandon Le Kestrel Rogers Chathura Gunasekara Haitang Duan Yiyou Gu Lei Tian Jinfu Nie Jian Qi Fanrong Meng Lan Huang Qinghui Chen Zhenlin Wang Jinshan Tang Xiaoqing Tang Ting Lan Xuemei Chen Hairong Wei Quanzhi Zhao Guiliang Tang 

作者机构：Collaborative Innovation Center of Henan Grain CropsHenan Agricultural UniversityZhengzhou 450002China College of Information and Electrical EngineeringChina Agricultural UniversityBeijing 100083China Department of Kinesiology and Integrative PhysiologyLife Science and Technology institutedMichigan Technological UniversityHoughtonMI 49931USA School of TechnologyMichigan Technological UniversityHoughtonMI 49931USA Guangdong Provincial Key Laboratory for Plant EpigeneticsCollege of Life Sciences and OceanographyShenzhen UniversityShenzhen 518060P.R.China Beijing Advanced Innovation Center for Tree Breeding by Molecular DesignBeijing Forestry UniversityBeijing 100083P.R.China Key Laboratory of Plant Stress BiologyState Key Laboratory of Cotton BiologySchool of Life SciencesHenan UniversityKaifeng 475004China Department of BiotechnologySharda UniversityGreater Noida201306India Key Laboratory of Rice Biology in Henan ProvinceHenan Agricultural UniversityZhengzhou 450002China Department of Biological SciencesUfe Science and Technology InstituteMichigan Technological UniversityHoughtonMI 49931USA Department of Botany and Plant SciencesInstitute of Integrative Genome BiologyUniversity of CaliforniaRiversideCA 92521USA School of Forest Resources and Environmental ScienceLife Science and Technology InstituteMichigan Technological UniversityHoughtonMI 49931USA Department of Computer ScienceMichigan Technological UniversityHoughtonMI 49931USA Anhui Province Key Laboratory of Medical Physics and TechnologyCenter of Medical Physics and TechnologyHefei Institutes of Physical ScienceChinese Academy of SciencesHefeiAnhuiChina Hefei Cancer HospitalChinese Academy of SciencesHefeiAnhuiChina Guangzhou Institute of Biomedicine and HealthChinese Academy of SciencesGuangzhouChina 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2018年第11卷第11期

页      面：1400-1417页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 0901[农学-作物学] 0902[农学-园艺学] 

基　　金：the National Science Foundation,USA (IOS-1048216 and IOS-1340001) the National Natural Science Foundation of China (31571679,31501292,31871554) the Major Science and Technology Project of Henan Province (141100110600) the Support Plan of Science and Technology Innovation Team in Universities of Henan Province (171RTSTHN015),and the Key Scientific Research Project in Universities of Henan Province (16A210009).G.T.is also supported by the Guangdong Innovation Research Team Fund (2014ZT058078)and the 111 Project (D16014)to Henan University.S.T.was supported by a post-doctoral fellowship from Henan Agricultural University.F.M.was a visiting scholar supported by the China Scholarship Council (CSC).T.P.,Z.Z.,L.S.,and L.T.were visiting PhD students supported by scholarships from Henan Agricultural University 

主　　题：short tandem target mimic (STTM) miRNA RNA-seq Arabidopsis crop 

摘      要：microRNAs (miRNAs)are endogenous small non-coding RNAs that bind to mRNAs and target them for cleavage and/or translational repression,leading to gene *** previously developed short tandem target mimic (STTM)technology to deactivate endogenous miRNAs in ***,we created hundreds of STTMs that target both conserved and species-specific miRNAs in Arabidopsis,tomato,rice,and maize,providing a resource for the functional interrogation of *** not only revealed the functions of several miRNAs in plant development,but also demonstrated that tissue-specific inactivation of a few miRNAs in rice leads to an increase in grain size without adversely affecting overall plant growth and ***-seq and small RNAseq analyses of STTM156/157 and STTM165/166 transgenic plants revealed the roles of these miRNAs in plant hormone biosynthesis and activation,secondary metabolism,and ion-channel activity-associated electrophysiology,demonstrating that STTM technology is an effective approach for studying miRNA *** facilitate the study and application of STTM transgenic plants and to provide a useful platform for storing and sharing of information about miRNA-regulated gene networks,we have established an online Genome Browser (https://***/***) to display the transcriptomic and miRNAomic changes in STTMinduced miRNA knockdown plants.